LOCUS       pGL4.11_Nued2_E3        8413 bp ds-DNA     circular     04-JUN-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: pGL4.11_Nued2_E3.gb
FEATURES             Location/Qualifiers
     CDS             406..525
                     /label="hPEST"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    577..698
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     rep_origin      complement(1117..1705)
                     /label="ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             complement(1905..2696)
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             complement(2697..2765)
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA"
     polyA_signal    2870..2918
                     /label="poly(A) signal"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2932..3023
                     /label="pause site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     iDNA            3856..4257
                     /label="DNAse1"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    3857..4668
                     /label="mIGF1 Enhancer 7"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3999..4005
                     /label="AP-1 BS, var 2.1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3999..4005
                     /label="AP-1 BS, canonical"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3999..4005
                     /label="AP-1 BS, var 2.1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3999..4005
                     /label="AP-1 BS, canonical"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3999..4005
                     /label="AP-1 BS, canonical"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(3999..4005)
                     /label="AP-1 BS, var 2.1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(3999..4005)
                     /label="AP-1 BS, var 2.1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(4685..4691)
                     /label="AP-1 BS, var 2.3"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    4716..4721
                     /label="Multiple cloning site for enhancers"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     regulatory      7160..7169
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             7166..402
                     /label="luciferase"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 tggctgcaca gcggcgacat cgcctactgg gacgaggacg agcacttctt catcgtggac
       61 cggctgaaga gcctgatcaa atacaagggc taccaggtag ccccagccga actggagagc
      121 atcctgctgc aacaccccaa catcttcgac gccggggtcg ccggcctgcc cgacgacgat
      181 gccggcgagc tgcccgccgc agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag
      241 gagatcgtgg actatgtggc cagccaggtt acaaccgcca agaagctgcg cggtggtgtt
      301 gtgttcgtgg acgaggtgcc taaaggactg accggcaagt tggacgcccg caagatccgc
      361 gagattctca ttaaggccaa gaagggcggc aagatcgccg tgaattctca cggcttccct
      421 cccgaggtgg aggagcaggc cgccggcacc ctgcccatga gctgcgccca ggagagcggc
      481 atggatagac accctgctgc ttgcgccagc gccaggatca acgtctaagg ccgcgactct
      541 agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat gagtttggac
      601 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      661 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      721 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      781 aatgtggtaa aatcgataag gatccgtcga ccgatgccct tgagagcctt caacccagtc
      841 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
      901 atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc
      961 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1021 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1081 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1141 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1201 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1261 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1321 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1381 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1441 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1501 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1561 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1621 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1681 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1741 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1801 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1861 aacttggtct gacagcggcc gcaaatgcta aaccactgca gtggttacca gtgcttgatc
     1921 agtgaggcac cgatctcagc gatctgccta tttcgttcgt ccatagtggc ctgactcccc
     1981 gtcgtgtaga tcactacgat tcgtgagggc ttaccatcag gccccagcgc agcaatgatg
     2041 ccgcgagagc cgcgttcacc ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg
     2101 gccgagcgaa gaagtggtcc tgctactttg tccgcctcca tccagtctat gagctgctgt
     2161 cgtgatgcta gagtaagaag ttcgccagtg agtagtttcc gaagagttgt ggccattgct
     2221 actggcatcg tggtatcacg ctcgtcgttc ggtatggctt cgttcaactc tggttcccag
     2281 cggtcaagcc gggtcacatg atcacccata ttatgaagaa atgcagtcag ctccttaggg
     2341 cctccgatcg ttgtcagaag taagttggcc gcggtgttgt cgctcatggt aatggcagca
     2401 ctacacaatt ctcttaccgt catgccatcc gtaagatgct tttccgtgac cggcgagtac
     2461 tcaaccaagt cgttttgtga gtagtgtata cggcgaccaa gctgctcttg cccggcgtct
     2521 atacgggaca acaccgcgcc acatagcagt actttgaaag tgctcatcat cgggaatcgt
     2581 tcttcggggc ggaaagactc aaggatcttg ccgctattga gatccagttc gatatagccc
     2641 actcttgcac ccagttgatc ttcagcatct tttactttca ccagcgtttc ggggtgtgca
     2701 aaaacaggca agcaaaatgc cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg
     2761 ctcatactcg tcctttttca atattattga agcatttatc agggttacta gtacgtctct
     2821 caaggataag taagtaatat taaggtacgg gaggtattgg acaggccgca ataaaatatc
     2881 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc
     2941 tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag
     3001 tgcaggtgcc agaacatttc tctggcctaa ctggccggta cctgagctcg ctagcttcct
     3061 ggcttgtagt gacctcgtct attgctagaa atcagaaggt accactgagc cttcatttgt
     3121 ttggtgaaat catggagtta cattaacaag ccgtttcccc aaactatggc tctctaccca
     3181 cttgtaatac acccgtgcac aggaacattt tctgaaagtc aatgtttcct atcacttgct
     3241 ccaaaattaa accagagata catcattcag gaagaagcca gatgcactga agaaagaaaa
     3301 acctgcttta tcagaagggg caagcttctc tcacatccca tagcaattta aattcatttg
     3361 ccggaatgtt aaatacaacc agtcttttag ttgggtccct tcacataaac accaagttag
     3421 cataaatgca aaatagttca catgattaaa tctttataac accataattt atcttatgta
     3481 cacacacaca cacagagaaa gagagagaga gagagagaga gagagatctt ttacttgctg
     3541 atacttctac attttttctg gagttctcaa ctctcatcat ctctctaagg aagctggctt
     3601 gtattgcccc agactcttag tgtatttagt tctaaaggat agtatacaga ataaagggaa
     3661 attttaattt tgtttttaat aggacctata taattcaggc ctagctctgg tggagagaag
     3721 gggaggagag gtccattagc atttattgtg cacctgcttt atgctaaaca tgtctggaaa
     3781 cagtgtctca caccataggc agtgagtgtc tagattttag aagtctagaa ctgagccctg
     3841 acttcatata attcatgtgc cctggcctta atcttggcac tttagtagtg ctaaaggtcc
     3901 ccaggccctc ccttagggtc agaacatggt taataagaga cacagggcat gtgattacag
     3961 actagcaaga gtgttacact cacaggtgcc tcagcccatg agtcatggct tgtagccttg
     4021 cattccaaga agttctggat ccctgcagct cagccaattt tggacattaa tgtccccaga
     4081 acaacaagta gatgctgtac tgtggccaag gcaacagcag ctgcttctga tgctaccatg
     4141 aggtaaagaa gggacacaaa actaaattca cctcatataa caaacaaacc agaagaaaaa
     4201 tcaattaaac ctccaatact ttttttattt ttataaccta aaaaactata tatacacctg
     4261 gtaaatatgc aatgtaatac agacaattgt gttttaattt aaattatttc tcacaagagg
     4321 cttttttaaa aagtatattc actaaaatat ttggatcatt gtgaaaactt catttactgc
     4381 ttgaaatatc taaaattctc tacctcgctt ggttgtatgg acacagttct gaattgcatt
     4441 tacctcaaag aaaaatacca gatatgctga tttgaaggaa aagaaaaaaa gcttggcatt
     4501 tgagagaaat aatgaacctc ctcaaagcat tccttttttt ttttttaaag agcttcagaa
     4561 aaatcaacaa gaaagaaagg ctgatatgca gtgcttttaa gcattctgtc agatttgacc
     4621 atgtacagat ccccacatat ccacagagtt gaaacatgtt ctctcaaatt taaagcagac
     4681 tacctgagtg atcaaaNNNN Ngtttaaacg tgtGGcgcgc cagctagtaa cagttggcat
     4741 tgggactaga aggcctcatt cctaaaccag cagatttttc cattgtacca ggaaaacaaa
     4801 actgcaaata gcataagtcc tctgatttta ctgtctccag agaaaatcat atcctcaatg
     4861 cctgggtctt tccatctgga atttggcaac tctgctgatc ccttctcata gtgggaactc
     4921 ttgtaggaag tctaagagca attattttgg ggatatatgc tatttttatg tcttcttgta
     4981 catcaccttc tttggtcagg aggaaattag aattctggaa atacgtttaa ttattaaaag
     5041 gacaagcctt ggcttcaagt agttgcaaat atgaattatt agtccatgtc atgaaagggc
     5101 tggagaacta caaagaaaca atgataatct atttgtcaca cccaattaag atctgccctg
     5161 gcctgggagt aaaagagcat taaatctgtg agtggtaact tcaagcagca tgtctatatt
     5221 tgggtaattc tgaacatgcc tggatttggt accttacagc atcctctaaa aatgtactac
     5281 tttagaaagt gttttgagat tttgttgcct tcgtgattgc agtttgtgtg tgtgtgtgtg
     5341 tgtggggggt gttatttgag cttttgagtt tccatgtagc caaagaaaga aagtctagga
     5401 agaaatggta gactcaaggt caaattcaac aaggggaaac ttctttgtgg tgagtctaga
     5461 caacaaagcc attagcccag tgttcacccc cctgcctaca ccctaccttt tccattgatg
     5521 aatattctta cctgagccat cctcgcttct gagtcctgct ctgggtcctg tggagactga
     5581 atatcctgtc tgtagttttt aatccttgaa gagatttttc atgaatagaa ctttccttat
     5641 aatttagcaa cactgtcctt gaaaaccccc acaactattt acataacaga agctattgtt
     5701 tgaagtgcaa aacaggtcag aatttggttc ccagagatta atcactgcag tctattttgt
     5761 attatcataa taatttcatt tattttgata caatttgttt tcagaatcaa ccccagcttc
     5821 tttgcacaag tgacaaatcc atttgtttct ggtagaatgg gtaatgtgat taatgacctt
     5881 gcaacaggat ttctttaaaa agggggaact tttttttttt catttttaat tcacagtaaa
     5941 ccagctgagt ctgttaagtg tgtaactgtt ctgaacttgt aagccatggg taagattttg
     6001 aaataccccc tcccaatctt ttgcggggtt tctgggtatt aatattaggc tttctccaag
     6061 aatctgatga tactagtctc caggtgtaaa gtggacttgt tggattcccg cattaaaaag
     6121 aacggggccc cagtctttgc catggcccca tcatgtttat tccggctgga tgttctcatc
     6181 aaaccgaggt catgcgaaat aaataaggtc atgcctagaa ggatattgtc cccgcacaag
     6241 aaccttcgag tgaactcgtg actactgcct cctggtcttc tgtgtgggca tccccttctt
     6301 tcttgcagca tccaggaccc acttctgtcc ctgctggtct gtttctttct ttctttcttt
     6361 ctttctttct ttctttcttg ttttcttccg tttttcccct tgagttctct ggggttgggc
     6421 ctgggatttt gggtacgcct acagactgtc cgcctctgca atcgaccgtc gtgcttcccg
     6481 cgccagaaca aggaagccca cgcgagccgc gtaggtggct cgcgtggaag acccgggttc
     6541 gagtcccgct gggagaattc ctcctaggca agtggagtca tctcggtcag gatttctcag
     6601 ccggaagccg cgccaacacc tctcatcgtg ggcggcaagt tgtccccggg gcttctttga
     6661 ccgtgcgggg ccagccgacg ctttctctga ccgtttccga agcccagttt cctttcctaa
     6721 ggagccttgg ggatcctgca ctcgcgtctc tgaaacccaa cccacgacag aacccagccg
     6781 cctgggccag cttagcaatg cgcccgcgcc ccgccctccg cgtgacgcga acgccggcgg
     6841 accaatcaga gagcgcggcg cgagccccac gcgcctgtgg ccccaccctc caagcttaag
     6901 aaagggcgcg cgggcagagc ccgggccaga acgccgcgca aagcggagag cgggtttttg
     6961 cgacggcagg cggcgcttgt gctgccgttt ccgtgctgca cgcggttccc tcgagtgccg
     7021 cgctcggaca gacgtagccg gccgcgaagg cgcccagacg gcaaaccagc gacccatgct
     7081 gaagtgagca cacaggtcag cgaggatatc aagatctggc ctcggcggcc aagcttggca
     7141 atccggtact gttggtaaag ccaccatgga agatgccaaa aacattaaga agggcccagc
     7201 gccattctac ccactcgaag acgggaccgc cggcgagcag ctgcacaaag ccatgaagcg
     7261 ctacgccctg gtgcccggca ccatcgcctt taccgacgca catatcgagg tggacattac
     7321 ctacgccgag tacttcgaga tgagcgttcg gctggcagaa gctatgaagc gctatgggct
     7381 gaatacaaac catcggatcg tggtgtgcag cgagaatagc ttgcagttct tcatgcccgt
     7441 gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct aacgacatct acaacgagcg
     7501 cgagctgctg aacagcatgg gcatcagcca gcccaccgtc gtattcgtga gcaagaaagg
     7561 gctgcaaaag atcctcaacg tgcaaaagaa gctaccgatc atacaaaaga tcatcatcat
     7621 ggatagcaag accgactacc agggcttcca aagcatgtac accttcgtga cttcccattt
     7681 gccacccggc ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg acaaaaccat
     7741 cgccctgatc atgaacagta gtggcagtac cggattgccc aagggcgtag ccctaccgca
     7801 ccgcaccgct tgtgtccgat tcagtcatgc ccgcgacccc atcttcggca accagatcat
     7861 ccccgacacc gctatcctca gcgtggtgcc atttcaccac ggcttcggca tgttcaccac
     7921 gctgggctac ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg aggaggagct
     7981 attcttgcgc agcttgcaag actataagat tcaatctgcc ctgctggtgc ccacactatt
     8041 tagcttcttc gctaagagca ctctcatcga caagtacgac ctaagcaact tgcacgagat
     8101 cgccagcggc ggggcgccgc tcagcaagga ggtaggtgag gccgtggcca aacgcttcca
     8161 cctaccaggc atccgccagg gctacggcct gacagaaaca accagcgcca ttctgatcac
     8221 ccccgaaggg gacgacaagc ctggcgcagt aggcaaggtg gtgcccttct tcgaggctaa
     8281 ggtggtggac ttggacaccg gtaagacact gggtgtgaac cagcgcggcg agctgtgcgt
     8341 ccgtggcccc atgatcatga gcggctacgt taacaacccc gaggctacaa acgctctcat
     8401 cgacaaggac ggc
//