LOCUS       pGL4.11_Nued2_E1_dAP2   8157 bp ds-DNA     circular     04-JUN-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            pGL4.11_Nued2_E1_dAP2.gb
FEATURES             Location/Qualifiers
     primer          complement(26..45)
                     /label="OR_pGL4.24_RVseq-3"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="sequence: gttaacgtagccgctcatga"
     primer          378..397
                     /label="OR_pGL4.24_RVseq-6"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="sequence: tggtgttgtgttcgtggacg"
     primer          complement(485..504)
                     /label="OR_pGL4.24_RVseq-5"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="sequence: agggaagccgtgagaattca"
     CDS             490..609
                     /label="hPEST"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    661..782
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          804..824
                     /label="OR_pGL4.24-1"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="sequence: tttatgtttcaggttcagggg"
     primer          complement(952..971)
                     /label="OR_pGL4.24-2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="sequence: ataagtgcggcgacgatagt"
     rep_origin      complement(1201..1789)
                     /label="ori"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             complement(1989..2780)
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             complement(2781..2849)
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA"
     polyA_signal    2954..3002
                     /label="poly(A) signal"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3016..3107
                     /label="pause site"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          3056..3075
                     /label="Promega_RVprimer3"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="sequence: ctagcaaaataggctgtccc"
     primer          3775..3794
                     /label="OR_pGL4.11_Nued2-1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="sequence: taattcaggcctagctctgg"
     primer          complement(3858..3877)
                     /label="OR_pGL4.11_Nued-Gibson-RV"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="sequence: gtgtgagacactgtttccag"
     PCR_primer      3878..3897
                     /label="138795315 IS_E18-3"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          3878..3897
                     /label="OR_mIgf1_E3_gibson_5"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="sequence: ttcactctacatcccaccca"
     PCR_primer      3878..3897
                     /label="139204309 IS_E18-7"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     PCR_primer      3894..3912
                     /label="138795316 IS_E18-4"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(3943..3966)
                     /label="74471436 OR_mIgf1_E2-16"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="sequence: tggatacctttgctggggctaggg"
     misc_feature    4049..4056
                     /label="Pou3f4 BS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     variation       4061..4067
                     /label="AP-1 BS, Var2-1 and Var1-1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4077..4099
                     /label="SM756.Igf1.g1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4088..4094
                     /label="AP-1 BS, canonical"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4091..4113
                     /label="SM756.Igf1.g2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4185..4192
                     /label="Pou3f4 BS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4213..4220
                     /label="Pou3f4 BS"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4250..4273
                     /label="SM757.Igf1.g1"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(4262..4277)
                     /label="SM757.Igf1.g5"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          4322..4344
                     /label="74471437 OR_mIgf1_E2-17"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="sequence: gcctgctgctaccctattcctga"
     PCR_primer      complement(4504..4523)
                     /label="138795317 IS_E18-5"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(4504..4523)
                     /label="OR_mIgf1_E3_gibson_6"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="sequence: cctcagtggtttggtctgtt"
     misc_feature    4544..4549
                     /label="Multiple cloning site for enhancers"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(4630..4650)
                     /label="OR_pGL4.11_Nued2-2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="sequence: gaggacttatgctatttgcag"
     primer          6959..6979
                     /label="OR_pGL4.11-Gibson-FW"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="sequence: aagcttggcaatccggtactg"
     regulatory      6988..6997
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             6994..486
                     /label="luciferase"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer          complement(7009..7028)
                     /label="OR_pGL4.11-1"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="sequence: gctgggcccttcttaatgtt"
     primer          7476..7495
                     /label="OR_pGL4.24_RVseq-2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="sequence: ccaaagcatgtacaccttcg"
     primer          complement(7580..7599)
                     /label="OR_pGL4.24_RVseq-1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="sequence: ggtactgccactactgttca"
     primer          8022..8041
                     /label="OR_pGL4.24_RVseq-4"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="sequence: agaaacaaccagcgccattc"
ORIGIN
        1 gagctgtgcg tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca
       61 aacgctctca tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag
      121 gacgagcact tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag
      181 gtagccccag ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg
      241 gtcgccggcc tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa
      301 cacggtaaaa ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc
      361 gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc
      421 aagttggacg cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc
      481 gccgtgaatt ctcacggctt ccctcccgag gtggaggagc aggccgccgg caccctgccc
      541 atgagctgcg cccaggagag cggcatggat agacaccctg ctgcttgcgc cagcgccagg
      601 atcaacgtct aaggccgcga ctctagagtc ggggcggccg gccgcttcga gcagacatga
      661 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      721 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      781 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
      841 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg
      901 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
      961 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     1021 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1081 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1141 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1201 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1261 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1321 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1381 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1441 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1501 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1561 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1621 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1681 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1741 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1801 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1861 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1921 atcaatctaa agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac
     1981 tgcagtggtt accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt
     2041 tcgtccatag tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca
     2101 tcaggcccca gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca
     2161 gcaatgaacc agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc
     2221 tccatccagt ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt
     2281 ttccgaagag ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg
     2341 gcttcgttca actctggttc ccagcggtca agccgggtca catgatcacc catattatga
     2401 agaaatgcag tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg
     2461 ttgtcgctca tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga
     2521 tgcttttccg tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga
     2581 ccaagctgct cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg
     2641 aaagtgctca tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta
     2701 ttgagatcca gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact
     2761 ttcaccagcg tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg
     2821 agtgcgacac gaaaatgttg gatgctcata ctcgtccttt ttcaatatta ttgaagcatt
     2881 tatcagggtt actagtacgt ctctcaagga taagtaagta atattaaggt acgggaggta
     2941 ttggacaggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
     3001 tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc
     3061 aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctggc ctaactggcc
     3121 ggtacctgag ctcgctagct tcctggcttg tagtgacctc gtctattgct agaaatcaga
     3181 aggtaccact gagccttcat ttgtttggtg aaatcatgga gttacattaa caagccgttt
     3241 ccccaaacta tggctctcta cccacttgta atacacccgt gcacaggaac attttctgaa
     3301 agtcaatgtt tcctatcact tgctccaaaa ttaaaccaga gatacatcat tcaggaagaa
     3361 gccagatgca ctgaagaaag aaaaacctgc tttatcagaa ggggcaagct tctctcacat
     3421 cccatagcaa tttaaattca tttgccggaa tgttaaatac aaccagtctt ttagttgggt
     3481 cccttcacat aaacaccaag ttagcataaa tgcaaaatag ttcacatgat taaatcttta
     3541 taacaccata atttatctta tgtacacaca cacacacaga gaaagagaga gagagagaga
     3601 gagagagaga tcttttactt gctgatactt ctacattttt tctggagttc tcaactctca
     3661 tcatctctct aaggaagctg gcttgtattg ccccagactc ttagtgtatt tagttctaaa
     3721 ggatagtata cagaataaag ggaaatttta attttgtttt taataggacc tatataattc
     3781 aggcctagct ctggtggaga gaaggggagg agaggtccat tagcatttat tgtgcacctg
     3841 ctttatgcta aacatgtctg gaaacagtgt ctcacacttc actctacatc ccacccactg
     3901 ctcctctcct attcaccctt tcccacaatc tttcccccat ccccctagcc ccagcaaagg
     3961 tatccatcat tggctgaaaa gtatacattt ttcagcatgg agcattactg ctgacaaaaa
     4021 cccactttag gaagtaagat ttcttctctt attcatagaa tgattcatgt cacacacact
     4081 tcttttttga ctcacacggt acgtcacaac aggctttgta aggcttataa ttactgaggc
     4141 tatttttgtg tctctattca ttgctttccc ccacccgcct tctctgcatt aataaccagt
     4201 ccatcccccc cccctcatta ttcagtacgc agcaaaatag aactggtgtc agccaatcAa
     4261 gcctcttgta tggtttttag gcagaagctt aaaaacattg gtccttgtca acagaaatta
     4321 agcctgctgc taccctattc ctgaggtaga tccaggaaaa ctctgaaata gaatgggaac
     4381 agatctcata tctaattaga tcctttctat ctctgagaat tgtattcagt gcaatcatta
     4441 ctgccacctg cttgacagtg tgtacaatac tttgtttaag cttccccact actggatcca
     4501 tttaacagac caaaccactg aggaagcgcg tttaaacgtg tGGcgcgcca gctagtaaca
     4561 gttggcattg ggactagaag gcctcattcc taaaccagca gatttttcca ttgtaccagg
     4621 aaaacaaaac tgcaaatagc ataagtcctc tgattttact gtctccagag aaaatcatat
     4681 cctcaatgcc tgggtctttc catctggaat ttggcaactc tgctgatccc ttctcatagt
     4741 gggaactctt gtaggaagtc taagagcaat tattttgggg atatatgcta tttttatgtc
     4801 ttcttgtaca tcaccttctt tggtcaggag gaaattagaa ttctggaaat acgtttaatt
     4861 attaaaagga caagccttgg cttcaagtag ttgcaaatat gaattattag tccatgtcat
     4921 gaaagggctg gagaactaca aagaaacaat gataatctat ttgtcacacc caattaagat
     4981 ctgccctggc ctgggagtaa aagagcatta aatctgtgag tggtaacttc aagcagcatg
     5041 tctatatttg ggtaattctg aacatgcctg gatttggtac cttacagcat cctctaaaaa
     5101 tgtactactt tagaaagtgt tttgagattt tgttgccttc gtgattgcag tttgtgtgtg
     5161 tgtgtgtgtg tggggggtgt tatttgagct tttgagtttc catgtagcca aagaaagaaa
     5221 gtctaggaag aaatggtaga ctcaaggtca aattcaacaa ggggaaactt ctttgtggtg
     5281 agtctagaca acaaagccat tagcccagtg ttcacccccc tgcctacacc ctaccttttc
     5341 cattgatgaa tattcttacc tgagccatcc tcgcttctga gtcctgctct gggtcctgtg
     5401 gagactgaat atcctgtctg tagtttttaa tccttgaaga gatttttcat gaatagaact
     5461 ttccttataa tttagcaaca ctgtccttga aaacccccac aactatttac ataacagaag
     5521 ctattgtttg aagtgcaaaa caggtcagaa tttggttccc agagattaat cactgcagtc
     5581 tattttgtat tatcataata atttcattta ttttgataca atttgttttc agaatcaacc
     5641 ccagcttctt tgcacaagtg acaaatccat ttgtttctgg tagaatgggt aatgtgatta
     5701 atgaccttgc aacaggattt ctttaaaaag ggggaacttt ttttttttca tttttaattc
     5761 acagtaaacc agctgagtct gttaagtgtg taactgttct gaacttgtaa gccatgggta
     5821 agattttgaa ataccccctc ccaatctttt gcggggtttc tgggtattaa tattaggctt
     5881 tctccaagaa tctgatgata ctagtctcca ggtgtaaagt ggacttgttg gattcccgca
     5941 ttaaaaagaa cggggcccca gtctttgcca tggccccatc atgtttattc cggctggatg
     6001 ttctcatcaa accgaggtca tgcgaaataa ataaggtcat gcctagaagg atattgtccc
     6061 cgcacaagaa ccttcgagtg aactcgtgac tactgcctcc tggtcttctg tgtgggcatc
     6121 cccttctttc ttgcagcatc caggacccac ttctgtccct gctggtctgt ttctttcttt
     6181 ctttctttct ttctttcttt ctttcttgtt ttcttccgtt tttccccttg agttctctgg
     6241 ggttgggcct gggattttgg gtacgcctac agactgtccg cctctgcaat cgaccgtcgt
     6301 gcttcccgcg ccagaacaag gaagcccacg cgagccgcgt aggtggctcg cgtggaagac
     6361 ccgggttcga gtcccgctgg gagaattcct cctaggcaag tggagtcatc tcggtcagga
     6421 tttctcagcc ggaagccgcg ccaacacctc tcatcgtggg cggcaagttg tccccggggc
     6481 ttctttgacc gtgcggggcc agccgacgct ttctctgacc gtttccgaag cccagtttcc
     6541 tttcctaagg agccttgggg atcctgcact cgcgtctctg aaacccaacc cacgacagaa
     6601 cccagccgcc tgggccagct tagcaatgcg cccgcgcccc gccctccgcg tgacgcgaac
     6661 gccggcggac caatcagaga gcgcggcgcg agccccacgc gcctgtggcc ccaccctcca
     6721 agcttaagaa agggcgcgcg ggcagagccc gggccagaac gccgcgcaaa gcggagagcg
     6781 ggtttttgcg acggcaggcg gcgcttgtgc tgccgtttcc gtgctgcacg cggttccctc
     6841 gagtgccgcg ctcggacaga cgtagccggc cgcgaaggcg cccagacggc aaaccagcga
     6901 cccatgctga agtgagcaca caggtcagcg aggatatcaa gatctggcct cggcggccaa
     6961 gcttggcaat ccggtactgt tggtaaagcc accatggaag atgccaaaaa cattaagaag
     7021 ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc
     7081 atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg
     7141 gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc
     7201 tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc
     7261 atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac
     7321 aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc
     7381 aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc
     7441 atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact
     7501 tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac
     7561 aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc
     7621 ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac
     7681 cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg
     7741 ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag
     7801 gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc
     7861 acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg
     7921 cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa
     7981 cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt
     8041 ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc
     8101 gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggc
//