LOCUS       pGL4.11_Nued2_E1_dAP1-2 8149 bp ds-DNA     circular     04-JUN-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            pGL4.11_Nued2_E1_dAP1-2.gb
FEATURES             Location/Qualifiers
     primer          complement(26..45)
                     /label="OR_pGL4.24_RVseq-3"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="sequence: gttaacgtagccgctcatga"
     primer          378..397
                     /label="OR_pGL4.24_RVseq-6"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="sequence: tggtgttgtgttcgtggacg"
     primer          complement(485..504)
                     /label="OR_pGL4.24_RVseq-5"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="sequence: agggaagccgtgagaattca"
     CDS             490..609
                     /label="hPEST"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    661..782
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          804..824
                     /label="OR_pGL4.24-1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: tttatgtttcaggttcagggg"
     primer          complement(952..971)
                     /label="OR_pGL4.24-2"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="sequence: ataagtgcggcgacgatagt"
     rep_origin      complement(1201..1789)
                     /label="ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(1989..2780)
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             complement(2781..2849)
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA"
     polyA_signal    2954..3002
                     /label="poly(A) signal"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3016..3107
                     /label="pause site"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          3056..3075
                     /label="Promega_RVprimer3"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="sequence: ctagcaaaataggctgtccc"
     primer          3775..3794
                     /label="OR_pGL4.11_Nued2-1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="sequence: taattcaggcctagctctgg"
     primer          complement(3858..3877)
                     /label="OR_pGL4.11_Nued-Gibson-RV"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="sequence: gtgtgagacactgtttccag"
     primer          3878..3897
                     /label="OR_mIgf1_E3_gibson_5"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="sequence: ttcactctacatcccaccca"
     PCR_primer      3878..3897
                     /label="139204309 IS_E18-7"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     PCR_primer      3878..3897
                     /label="138795315 IS_E18-3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     PCR_primer      3894..3912
                     /label="138795316 IS_E18-4"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(3943..3966)
                     /label="74471436 OR_mIgf1_E2-16"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: tggatacctttgctggggctaggg"
     misc_feature    4049..4056
                     /label="Pou3f4 BS"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     variation       4061..4067
                     /label="AP-1 BS, Var2-1 and Var1-1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4077..4092
                     /label="SM756.Igf1.g1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4088..4106
                     /label="SM756.Igf1.g2"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    4178..4185
                     /label="Pou3f4 BS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4206..4213
                     /label="Pou3f4 BS"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4243..4265
                     /label="SM757.Igf1.g1"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(4254..4269)
                     /label="SM757.Igf1.g5"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          4314..4336
                     /label="74471437 OR_mIgf1_E2-17"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="sequence: gcctgctgctaccctattcctga"
     PCR_primer      complement(4496..4515)
                     /label="138795317 IS_E18-5"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(4496..4515)
                     /label="OR_mIgf1_E3_gibson_6"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="sequence: cctcagtggtttggtctgtt"
     misc_feature    4536..4541
                     /label="Multiple cloning site for enhancers"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(4622..4642)
                     /label="OR_pGL4.11_Nued2-2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="sequence: gaggacttatgctatttgcag"
     primer          6951..6971
                     /label="OR_pGL4.11-Gibson-FW"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="sequence: aagcttggcaatccggtactg"
     regulatory      6980..6989
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             6986..486
                     /label="luciferase"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer          complement(7001..7020)
                     /label="OR_pGL4.11-1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="sequence: gctgggcccttcttaatgtt"
     primer          7468..7487
                     /label="OR_pGL4.24_RVseq-2"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="sequence: ccaaagcatgtacaccttcg"
     primer          complement(7572..7591)
                     /label="OR_pGL4.24_RVseq-1"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="sequence: ggtactgccactactgttca"
     primer          8014..8033
                     /label="OR_pGL4.24_RVseq-4"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="sequence: agaaacaaccagcgccattc"
ORIGIN
        1 gagctgtgcg tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca
       61 aacgctctca tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag
      121 gacgagcact tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag
      181 gtagccccag ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg
      241 gtcgccggcc tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa
      301 cacggtaaaa ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc
      361 gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc
      421 aagttggacg cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc
      481 gccgtgaatt ctcacggctt ccctcccgag gtggaggagc aggccgccgg caccctgccc
      541 atgagctgcg cccaggagag cggcatggat agacaccctg ctgcttgcgc cagcgccagg
      601 atcaacgtct aaggccgcga ctctagagtc ggggcggccg gccgcttcga gcagacatga
      661 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      721 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      781 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
      841 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg
      901 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
      961 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     1021 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1081 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1141 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1201 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1261 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1321 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1381 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1441 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1501 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1561 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1621 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1681 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1741 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1801 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1861 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1921 atcaatctaa agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac
     1981 tgcagtggtt accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt
     2041 tcgtccatag tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca
     2101 tcaggcccca gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca
     2161 gcaatgaacc agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc
     2221 tccatccagt ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt
     2281 ttccgaagag ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg
     2341 gcttcgttca actctggttc ccagcggtca agccgggtca catgatcacc catattatga
     2401 agaaatgcag tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg
     2461 ttgtcgctca tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga
     2521 tgcttttccg tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga
     2581 ccaagctgct cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg
     2641 aaagtgctca tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta
     2701 ttgagatcca gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact
     2761 ttcaccagcg tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg
     2821 agtgcgacac gaaaatgttg gatgctcata ctcgtccttt ttcaatatta ttgaagcatt
     2881 tatcagggtt actagtacgt ctctcaagga taagtaagta atattaaggt acgggaggta
     2941 ttggacaggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
     3001 tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc
     3061 aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctggc ctaactggcc
     3121 ggtacctgag ctcgctagct tcctggcttg tagtgacctc gtctattgct agaaatcaga
     3181 aggtaccact gagccttcat ttgtttggtg aaatcatgga gttacattaa caagccgttt
     3241 ccccaaacta tggctctcta cccacttgta atacacccgt gcacaggaac attttctgaa
     3301 agtcaatgtt tcctatcact tgctccaaaa ttaaaccaga gatacatcat tcaggaagaa
     3361 gccagatgca ctgaagaaag aaaaacctgc tttatcagaa ggggcaagct tctctcacat
     3421 cccatagcaa tttaaattca tttgccggaa tgttaaatac aaccagtctt ttagttgggt
     3481 cccttcacat aaacaccaag ttagcataaa tgcaaaatag ttcacatgat taaatcttta
     3541 taacaccata atttatctta tgtacacaca cacacacaga gaaagagaga gagagagaga
     3601 gagagagaga tcttttactt gctgatactt ctacattttt tctggagttc tcaactctca
     3661 tcatctctct aaggaagctg gcttgtattg ccccagactc ttagtgtatt tagttctaaa
     3721 ggatagtata cagaataaag ggaaatttta attttgtttt taataggacc tatataattc
     3781 aggcctagct ctggtggaga gaaggggagg agaggtccat tagcatttat tgtgcacctg
     3841 ctttatgcta aacatgtctg gaaacagtgt ctcacacttc actctacatc ccacccactg
     3901 ctcctctcct attcaccctt tcccacaatc tttcccccat ccccctagcc ccagcaaagg
     3961 tatccatcat tggctgaaaa gtatacattt ttcagcatgg agcattactg ctgacaaaaa
     4021 cccactttag gaagtaagat ttcttctctt attcatagaa tgattcatgt cacacacact
     4081 tctttttcac ggtacgtcac aacaggcttt gtaaggctta taattactga ggctattttt
     4141 gtgtctctat tcattgcttt cccccacccg ccttctctgc attaataacc agtccatccc
     4201 ccccccctca ttattcagta cgcagcaaaa tagaactggt gtcagccaat cagcctcttg
     4261 tatggttttt aggcagaagc ttaaaaacat tggtccttgt caacagaaat taagcctgct
     4321 gctaccctat tcctgaggta gatccaggaa aactctgaaa tagaatggga acagatctca
     4381 tatctaatta gatcctttct atctctgaga attgtattca gtgcaatcat tactgccacc
     4441 tgcttgacag tgtgtacaat actttgttta agcttcccca ctactggatc catttaacag
     4501 accaaaccac tgaggaagcg cgtttaaacg tgtGGcgcgc cagctagtaa cagttggcat
     4561 tgggactaga aggcctcatt cctaaaccag cagatttttc cattgtacca ggaaaacaaa
     4621 actgcaaata gcataagtcc tctgatttta ctgtctccag agaaaatcat atcctcaatg
     4681 cctgggtctt tccatctgga atttggcaac tctgctgatc ccttctcata gtgggaactc
     4741 ttgtaggaag tctaagagca attattttgg ggatatatgc tatttttatg tcttcttgta
     4801 catcaccttc tttggtcagg aggaaattag aattctggaa atacgtttaa ttattaaaag
     4861 gacaagcctt ggcttcaagt agttgcaaat atgaattatt agtccatgtc atgaaagggc
     4921 tggagaacta caaagaaaca atgataatct atttgtcaca cccaattaag atctgccctg
     4981 gcctgggagt aaaagagcat taaatctgtg agtggtaact tcaagcagca tgtctatatt
     5041 tgggtaattc tgaacatgcc tggatttggt accttacagc atcctctaaa aatgtactac
     5101 tttagaaagt gttttgagat tttgttgcct tcgtgattgc agtttgtgtg tgtgtgtgtg
     5161 tgtggggggt gttatttgag cttttgagtt tccatgtagc caaagaaaga aagtctagga
     5221 agaaatggta gactcaaggt caaattcaac aaggggaaac ttctttgtgg tgagtctaga
     5281 caacaaagcc attagcccag tgttcacccc cctgcctaca ccctaccttt tccattgatg
     5341 aatattctta cctgagccat cctcgcttct gagtcctgct ctgggtcctg tggagactga
     5401 atatcctgtc tgtagttttt aatccttgaa gagatttttc atgaatagaa ctttccttat
     5461 aatttagcaa cactgtcctt gaaaaccccc acaactattt acataacaga agctattgtt
     5521 tgaagtgcaa aacaggtcag aatttggttc ccagagatta atcactgcag tctattttgt
     5581 attatcataa taatttcatt tattttgata caatttgttt tcagaatcaa ccccagcttc
     5641 tttgcacaag tgacaaatcc atttgtttct ggtagaatgg gtaatgtgat taatgacctt
     5701 gcaacaggat ttctttaaaa agggggaact tttttttttt catttttaat tcacagtaaa
     5761 ccagctgagt ctgttaagtg tgtaactgtt ctgaacttgt aagccatggg taagattttg
     5821 aaataccccc tcccaatctt ttgcggggtt tctgggtatt aatattaggc tttctccaag
     5881 aatctgatga tactagtctc caggtgtaaa gtggacttgt tggattcccg cattaaaaag
     5941 aacggggccc cagtctttgc catggcccca tcatgtttat tccggctgga tgttctcatc
     6001 aaaccgaggt catgcgaaat aaataaggtc atgcctagaa ggatattgtc cccgcacaag
     6061 aaccttcgag tgaactcgtg actactgcct cctggtcttc tgtgtgggca tccccttctt
     6121 tcttgcagca tccaggaccc acttctgtcc ctgctggtct gtttctttct ttctttcttt
     6181 ctttctttct ttctttcttg ttttcttccg tttttcccct tgagttctct ggggttgggc
     6241 ctgggatttt gggtacgcct acagactgtc cgcctctgca atcgaccgtc gtgcttcccg
     6301 cgccagaaca aggaagccca cgcgagccgc gtaggtggct cgcgtggaag acccgggttc
     6361 gagtcccgct gggagaattc ctcctaggca agtggagtca tctcggtcag gatttctcag
     6421 ccggaagccg cgccaacacc tctcatcgtg ggcggcaagt tgtccccggg gcttctttga
     6481 ccgtgcgggg ccagccgacg ctttctctga ccgtttccga agcccagttt cctttcctaa
     6541 ggagccttgg ggatcctgca ctcgcgtctc tgaaacccaa cccacgacag aacccagccg
     6601 cctgggccag cttagcaatg cgcccgcgcc ccgccctccg cgtgacgcga acgccggcgg
     6661 accaatcaga gagcgcggcg cgagccccac gcgcctgtgg ccccaccctc caagcttaag
     6721 aaagggcgcg cgggcagagc ccgggccaga acgccgcgca aagcggagag cgggtttttg
     6781 cgacggcagg cggcgcttgt gctgccgttt ccgtgctgca cgcggttccc tcgagtgccg
     6841 cgctcggaca gacgtagccg gccgcgaagg cgcccagacg gcaaaccagc gacccatgct
     6901 gaagtgagca cacaggtcag cgaggatatc aagatctggc ctcggcggcc aagcttggca
     6961 atccggtact gttggtaaag ccaccatgga agatgccaaa aacattaaga agggcccagc
     7021 gccattctac ccactcgaag acgggaccgc cggcgagcag ctgcacaaag ccatgaagcg
     7081 ctacgccctg gtgcccggca ccatcgcctt taccgacgca catatcgagg tggacattac
     7141 ctacgccgag tacttcgaga tgagcgttcg gctggcagaa gctatgaagc gctatgggct
     7201 gaatacaaac catcggatcg tggtgtgcag cgagaatagc ttgcagttct tcatgcccgt
     7261 gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct aacgacatct acaacgagcg
     7321 cgagctgctg aacagcatgg gcatcagcca gcccaccgtc gtattcgtga gcaagaaagg
     7381 gctgcaaaag atcctcaacg tgcaaaagaa gctaccgatc atacaaaaga tcatcatcat
     7441 ggatagcaag accgactacc agggcttcca aagcatgtac accttcgtga cttcccattt
     7501 gccacccggc ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg acaaaaccat
     7561 cgccctgatc atgaacagta gtggcagtac cggattgccc aagggcgtag ccctaccgca
     7621 ccgcaccgct tgtgtccgat tcagtcatgc ccgcgacccc atcttcggca accagatcat
     7681 ccccgacacc gctatcctca gcgtggtgcc atttcaccac ggcttcggca tgttcaccac
     7741 gctgggctac ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg aggaggagct
     7801 attcttgcgc agcttgcaag actataagat tcaatctgcc ctgctggtgc ccacactatt
     7861 tagcttcttc gctaagagca ctctcatcga caagtacgac ctaagcaact tgcacgagat
     7921 cgccagcggc ggggcgccgc tcagcaagga ggtaggtgag gccgtggcca aacgcttcca
     7981 cctaccaggc atccgccagg gctacggcct gacagaaaca accagcgcca ttctgatcac
     8041 ccccgaaggg gacgacaagc ctggcgcagt aggcaaggtg gtgcccttct tcgaggctaa
     8101 ggtggtggac ttggacaccg gtaagacact gggtgtgaac cagcgcggc
//