LOCUS       pAAV-Ef1a-fDIO-eGFP-2A- 7448 bp ds-DNA     circular     04-JUN-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE.gb
FEATURES             Location/Qualifiers
     promoter        249..1419
                     /label="EF1a_promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1247..1267
                     /label="224565890_EZ_Ef1a_Fwd"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="sequence: ggttttatgcgatggagtttc"
     misc_feature    1367..1387
                     /label="EF1a_fwd_primer"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1367..1387
                     /label="225790715_EZ_EF1a_fwd_primer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="sequence: tcaagcctcagacagtggttc"
     primer          1420..1440
                     /label="222188113_OR_AAV-fDIO_Gibson-7"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="sequence: ggtaccggatcctctagagtc"
     misc_feature    1450..1483
                     /label="FRT"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1534..1567
                     /label="FRT2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(1552..1571)
                     /label="OR_AAV-fDIO_Gibson-1"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="sequence: cgccgaagttcctatacctt"
     primer          1576..1595
                     /label="OR_Cre-NLS-3"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="sequence: tagaagttcctattcttcaaaaggtataggaacttcggcgcgccCTAATCGCCATCTTCCAGCA"
     primer          1576..1595
                     /label="OR_Cre-NLS-1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="sequence: CTAATCGCCATCTTCCAGCA"
     misc_feature    1576..1596
                     /label="Primer"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(1576..2604)
                     /label="Cre"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDGD*"
     misc_feature    1576..2703
                     /label="insert origin p632"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(1607..1626)
                     /label="delete"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="sequence: CTGGATAGTGAAACAGGGGC"
     primer          complement(1686..1705)
                     /label="225790716_EZ_Cre_Fwd_3"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="sequence: CGCTGGAGTTTCAATACCGG"
     primer          complement(1765..1784)
                     /label="224892688_EZ_Cre_Fwd_2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="sequence: CTAAGGATGACTCTGGTCAG"
     primer          1859..1878
                     /label="IS_CreKazu23"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="sequence: GGCGCGGCAACACCATTTTT"
     primer          2557..2577
                     /label="IS_11642"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="sequence: ATCGACCGGTAATGCAGGCAA"
     primer          complement(2605..2625)
                     /label="OR_Cre-NLS-4"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="sequence: gaagtctgctaacatgcggtgacgtcgaggagaatcctggcccaAGCGCTTTCAGAACCcccaagaagaagaggaaggtg"
     CDS             complement(2605..2625)
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="PKKKRKV"
     misc_feature    complement(2641..2703)
                     /label="2A"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(2685..2703)
                     /label="Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(2704..3420)
                     /label="eGFP"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2746..2767)
                     /label="75611292_IS_EGFP-C"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="sequence: CATGGTCCTGCTGGAGTTCGTG"
     primer          complement(2754..2772)
                     /label="226301567_EZ_eGFP_Fwd_1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="sequence: GATCACATGGTCCTGCTGG"
     primer          complement(2905..2926)
                     /label="75560308_MT_GFP_FWD"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="sequence: CTTCAAGATCCGCCACAACATC"
     primer          3095..3114
                     /label="225564047_EXFP-R_(eGFP_Rev)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="sequence: GTCTTGTAGTTGCCGTCGTC"
     primer          3270..3289
                     /label="75560307_MT_GFP_Rev"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="sequence: tggtgcagatgaacttcagg"
     primer          3370..3387
                     /label="226301568_EZ_eGFP_Rev_1"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="sequence: GACCAGGATGGGCACCAC"
     misc_feature    complement(3433..3466)
                     /label="FRT"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(3490..3511)
                     /label="224892692_EZ_fDIO-d/Cre_Seq"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="sequence: gcaccattctaaagaataacag"
     misc_feature    complement(3517..3550)
                     /label="FRT2"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3577..4164
                     /label="WPRE"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(3742..3761)
                     /label="75794531_AAVTitr_WPRE-rev"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="sequence: CGGGCCACAACTCCTCATAA"
     primer          complement(4032..4049)
                     /label="77631386_OR_WPRE_R_Tit"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="sequence: ccgaagggacgtagcaga"
     terminator      4193..4671
                     /label="hGH_PA_terminator"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(4652..4669)
                     /label="224592105_EZ_hGH-PA-Terminator_Rev"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="sequence: ggacagggaagggagcag"
     rep_origin      4943..5249
                     /label="f1_origin"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    5517..5539
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        5698..5726
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     gene            5768..6628
                     /label="Ampicillin"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             5768..6628
                     /label="ORF frame 2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     rep_origin      6783..7402
                     /label="pBR322_origin"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagagtc
     1441 gactccggag aagttcctat tctctagaaa gtataggaac ttcgcagaat ggtagctgga
     1501 ttgtagctgc tattagcaat atgaaacctc ttagaagttc ctattcttca aaaggtatag
     1561 gaacttcggc gcgccCTAAT CGCCATCTTC CAGCAGGCGC ACCATTGCCC CTGTTTCACT
     1621 ATCCAGGTTA CGGATATAGT TCATGACAAT ATTTACATTG GTCCAGCCAC CAGCTTGCAT
     1681 GATCTCCGGT ATTGAAACTC CAGCGCGGGC CATATCTCGC GCGGCTCCGA CACGGGCACT
     1741 GTGTCCAGAC CAGGCCAGGT ATCTCTGACC AGAGTCATCC TTAGCGCCGT AAATCAATCG
     1801 ATGAGTTGCT TCAAAAATCC CTTCCAGGGC GCGAGTTGAT AGCTGGCTGG TGGCAGATGG
     1861 CGCGGCAACA CCATTTTTTC TGACCCGGCA AAACAGGTAG TTATTCGGAT CATCAGCTAC
     1921 ACCAGAGACG GAAATCCATC GCTCGACCAG TTTAGTTACC CCCAGGCTAA GTGCCTTCTC
     1981 TACACCTGCG GTGCTAACCA GCGTTTTCGT TCTGCCAATA TGGATTAACA TTCTCCCACC
     2041 GTCAGTACGT GAGATATCTT TAACCCTGAT CCTGGCAATT TCGGCTATAC GTAACAGGGT
     2101 GTTATAAGCA ATCCCCAGAA ATGCCAGATT ACGTATATCC TGGCAGCGAT CGCTATTTTC
     2161 CATGAGTGAA CGAACCTGGT CGAAATCAGT GCGTTCGAAC GCTAGAGCCT GTTTTGCACG
     2221 TTCACCGGCA TCAACGTTTT CTTTTCGGAT CCGCCGCATA ACCAGTGAAA CAGCATTGCT
     2281 GTCACTTGGT CGTGGCAGCC CGGACCGACG ATGAAGCATG TTTAGCTGGC CCAAATGTTG
     2341 CTGGATAGTT TTTACTGCCA GACCGCGCGC CTGAAGATAT AGAAGATAAT CGCGAACATC
     2401 TTCAGGTTCT GCGGGAAACC ATTTCCGGTT ATTCAACTTG CACCATGCCG CCCACGACCG
     2461 GCAAACGGAC AGAAGCATTT TCCAGGTATG CTCAGAAAAC GCCTGGCGAT CCCTGAACAT
     2521 GTCCATCAGG TTCTTGCGAA CCTCATCACT CGTTGCATCG ACCGGTAATG CAGGCAAATT
     2581 TTGGTGTACG GTCAGTAAAT TGGACACCTT CCTCTTCTTC TTGGGGGTTC TGAAAGCGCT
     2641 TGGGCCAGGA TTCTCCTCGA CGTCACCGCA TGTTAGCAGA CTTCCTCTGC CCTCTCCACT
     2701 GCCCTTGTAC AGCTCGTCCA TGCCGAGaGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG
     2761 GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGcTCAGG GCGGACTGGG TGcTCAGGTA
     2821 GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC
     2881 GGCGAGCTGC ACGCTGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTCA CCTTGATGCC
     2941 GTTCTTCTGC TTGTCGGCCA TGATATAGAC GTTGTgGCTG TTGTAGTTGT ACTCCAGCTT
     3001 GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC
     3061 CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA
     3121 GAAGATGGTG CGCTCCTGGA CGTAGCCTTc gGGCATGGCG GAcTTGAAgA AgTCGTGCTG
     3181 CTTcATGTGG TCGGGGTAGC GGCTGAAGCA CTGCACGCCG TAGGTCAGGG TGGTCACGAG
     3241 GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATG AACTTCAGGG TCAGCTTGCC
     3301 GTAGGTGGCA TCGCCCTCGC CCTCGCCGGA CACGCTGAAC TTGTGGCCGT TTACGTCGCC
     3361 GTCCAGCTCG ACCAGGATGG GCACCACCCC GGTGAACAGC TCCTCGCCCT TGCTCACCAT
     3421 GGTGGCgcta gcgaagttcc tatactttct agagaatagg aacttcttgc cttaacccag
     3481 aaattatcac tgttattctt tagaatggtg caaagagaag ttcctatacc ttttgaagaa
     3541 taggaacttc gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     3601 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     3661 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     3721 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     3781 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     3841 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     3901 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     3961 gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg
     4021 cgggacgtcc ttctgctacg tcccttcggc cCtcaatcca gcggaccttc ctccccgcgg
     4081 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     4141 ctccctttgg gccgcctccc cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg
     4201 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc
     4261 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat
     4321 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt
     4381 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact
     4441 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga
     4501 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc
     4561 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct
     4621 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgattttg
     4681 taggtaacca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
     4741 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     4801 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
     4861 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     4921 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4981 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5041 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5101 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     5161 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5221 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga
     5281 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5341 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     5401 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     5461 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     5521 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     5581 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     5641 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5701 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5761 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5821 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5881 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5941 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     6001 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     6061 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     6121 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     6181 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     6241 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     6301 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6361 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     6421 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     6481 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     6541 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     6601 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6661 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6721 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6781 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6841 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6901 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     6961 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7021 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7081 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7141 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7201 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7261 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7321 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7381 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7441 tcacatgt
//