LOCUS       pAAV-Ef1a-fDIO-mRuby3-2 6832 bp ds-DNA     circular     04-JUN-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            pAAV-Ef1a-fDIO-mRuby3-2A-dCre.gb
FEATURES             Location/Qualifiers
     promoter        249..1419
                     /label="EF1a_promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1247..1267
                     /label="224565890_EZ_Ef1a_Fwd"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="sequence: ggttttatgcgatggagtttc"
     misc_feature    1367..1387
                     /label="EF1a_fwd_primer"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1420..1440
                     /label="222188113_OR_AAV-fDIO_Gibson-7"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="sequence: ggtaccggatcctctagagtc"
     misc_feature    1450..1483
                     /label="FRT"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1534..1567
                     /label="FRT2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(1552..1571)
                     /label="OR_AAV-fDIO_Gibson-1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="sequence: cgccgaagttcctatacctt"
     primer          1561..1594
                     /label="224892691_EZ_fDIO_dCre_AscI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="sequence: gaacttcggcgcgccCTACTTACGGATTCGCCGC"
     misc_feature    1576..1594
                     /label="Primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1576..1962)
                     /label="dCre-NLS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(1639..1657)
                     /label="224892689_EZ_dCre_Fwd"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="sequence: CATGCTTCATCGTCGGTCC"
     primer          complement(1883..1902)
                     /label="OR_AAV-fDIO_Gibson-9"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="sequence: GTCCAATTTACTGACCGTACACCAAAATTTGCCTGCATTACCGGTCGATGCAACGAGTGA"
     primer          complement(1942..1962)
                     /label="OR_Cre-NLS-4"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: gaagtctgctaacatgcggtgacgtcgaggagaatcctggcccaAGCGCTTTCAGAACCcccaagaagaagaggaaggtg"
     CDS             complement(1942..1962)
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="PKKKRKV"
     misc_feature    complement(1978..2040)
                     /label="2A"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(1999..2018)
                     /label="224892686_EZ_2A_Fwd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="sequence: GTCTGCTAACATGCGGTGAC"
     misc_feature    complement(2022..2040)
                     /label="Primer"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2041..2060
                     /label="Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2041..2060
                     /label="IS_mRuby3NLS-15 224724553"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(2041..2060)
                     /label="IS_CreNLS-1 224702016"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2041..2751
                     /label="insert origin p646"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(2041..2751)
                     /label="mRuby3"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMRIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGVVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDIALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVAKYSNLGGGMDELYK"
     misc_feature    complement(2041..2781)
                     /label="from pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             complement(2041..2784)
                     /label="Translation 2784-2041"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MPKKKRKVAAAMVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMRIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGVVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDIALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVAKYSNLGGGMDELYK"
     misc_feature    complement(2731..2751)
                     /label="IS_mRuby3NLS-3"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(2731..2784)
                     /label="IS_mRuby3NLS-14 RE00517611"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(2731..2794)
                     /label="RE00517611_IS_mRuby3NLS-14"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="sequence: cgataagcttgattcgagctagccaccATGCCCAAGAAGAAGAGGAAGGTGGCGGCCGCTATGGTGTCTAAGGGCGAAGAG"
     CDS             complement(2761..2781)
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="PKKKRKV"
     misc_feature    2795..1575
                     /label="Vector (origin: p#574)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(2795..2828)
                     /label="FRT"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(2852..2873)
                     /label="224892692_EZ_fDIO-d/Cre_Seq"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="sequence: gcaccattctaaagaataacag"
     misc_feature    complement(2879..2912)
                     /label="FRT2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2939..3548
                     /label="WPRE"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(3104..3123)
                     /label="75794531_AAVTitr_WPRE-rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="sequence: CGGGCCACAACTCCTCATAA"
     primer          complement(3394..3411)
                     /label="77631386_OR_WPRE_R_Tit"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="sequence: ccgaagggacgtagcaga"
     terminator      3577..4055
                     /label="hGH_PA_terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(4036..4053)
                     /label="224592105_EZ_hGH-PA-Terminator_Rev"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="sequence: ggacagggaagggagcag"
     rep_origin      4327..4633
                     /label="f1_origin"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4901..4923
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     promoter        5082..5110
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     gene            5152..6012
                     /label="Ampicillin"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             5152..6012
                     /label="ORF frame 2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     rep_origin      6167..6786
                     /label="pBR322_origin"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagagtc
     1441 gactccggag aagttcctat tctctagaaa gtataggaac ttcgcagaat ggtagctgga
     1501 ttgtagctgc tattagcaat atgaaacctc ttagaagttc ctattcttca aaaggtatag
     1561 gaacttcggc gcgccCTACT TACGGATTCG CCGCATAACC AGTGAAACAG CATTGCTGTC
     1621 ACTTGGTCGT GGCAGCCCGG ACCGACGATG AAGCATGTTT AGCTGGCCCA AATGTTGCTG
     1681 GATAGTTTTT ACTGCCAGAC CGCGCGCCTG AAGATATAGA AGATAATCGC GAACATCTTC
     1741 AGGTTCTGCG GGAAACCATT TCCGGTTATT CAACTTGCAC CATGCCGCCC ACGACCGGCA
     1801 AACGGACAGA AGCATTTTCC AGGTATGCTC AGAAAACGCC TGGCGATCCC TGAACATGTC
     1861 CATCAGGTTC TTGCGAACCT CATCACTCGT TGCATCGACC GGTAATGCAG GCAAATTTTG
     1921 GTGTACGGTC AGTAAATTGG ACACCTTCCT CTTCTTCTTG GGGGTTCTGA AAGCGCTTGG
     1981 GCCAGGATTC TCCTCGACGT CACCGCATGT TAGCAGACTT CCTCTGCCCT CTCCACTGCC
     2041 CTTGTACAGC TCGTCCATGC CACCACCAAG GTTGCTGTAT TTGGCAACTG CCACTTCTCT
     2101 TTGCACTACG TAGGTTTCAT TGTCACTCTC CTCGATCCTT TCCAGGCGGT GATCAACGGC
     2161 ATGGACACCG GGCATCTTGA TGTTCCCGAC GGTCTTTTTT GACCTGTAAG TTGTCACGAA
     2221 GTTGCAGTGC AGATGGCCAC CACCATCAAC TTTCAGTGCG ATGTCAGTGT ATCCTCTCAG
     2281 ACCACCATCT GCTGGATACA TCATCTCTGT ATTAGGCTCC CAACCCTTGG TCTTCTTCTG
     2341 CATCACGGGA CCATTGGAGG GAAAGTTTAC CCCTCTGACC TTGACGTTGT AGACGAGCTC
     2401 GCCATCCTCA AGGCTGGTGT CCTGCGTGAC GGTGACGACT CCACCATCTT CGTATCTCGT
     2461 AACTCTTTCC CAAGTAAAAC CCTCAGGAAA GGACTGTTTA AAGAAATCAG GGATGTCGGC
     2521 CGGGTACTTG ATAAAGGTAC GGCTGCCATA CATGAACGAC GTGGCAAGAA TGTCAAAGGC
     2581 AAATGGCAGG GGTCCTCCCT CGATGACTTT GATCCTCATG GTTTGCACTC CCTCGTACGG
     2641 TCTGCCTTCT CCTTCACCTG TGCATTTGAA TTGGTGGCCG TTGACCGAAC CTTCCATGAC
     2701 CACCTTCATA CGCATATTTT CCTTGATCAG CTCTTCGCCC TTAGACACCA TAGCGGCCGC
     2761 CACCTTCCTC TTCTTCTTGG GCATggtggc tagcgaagtt cctatacttt ctagagaata
     2821 ggaacttctt gccttaaccc agaaattatc actgttattc tttagaatgg tgcaaagaga
     2881 agttcctata ccttttgaag aataggaact tcgaattcga tatcaagctt atcgataatc
     2941 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     3001 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     3061 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     3121 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     3181 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     3241 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     3301 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     3361 atgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gcccCCAAGG
     3421 AAAGGACGAT GATTTCtcaa tccagcggac cttcctcccc gcggcctgct gccggctctg
     3481 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3541 tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc tgtgacccct
     3601 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa
     3661 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga
     3721 ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct
     3781 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct
     3841 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga
     3901 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc
     3961 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat
     4021 tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta accacgtgcg
     4081 gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     4141 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     4201 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
     4261 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     4321 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4381 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4441 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4501 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     4561 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4621 caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg
     4681 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4741 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     4801 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     4861 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     4921 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     4981 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5041 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5101 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5161 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5221 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5281 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5341 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     5401 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5461 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5521 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5581 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5641 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     5701 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5761 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5821 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5881 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5941 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6001 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6061 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6121 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6181 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6241 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6301 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     6361 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6421 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6481 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6541 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6601 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6661 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6721 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6781 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//