LOCUS       pAAV-hSyn-Flex-mRuby3-2 5287 bp ds-DNA     circular     04-JUN-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            pAAV-hSyn-Flex-mRuby3-2A.gb
FEATURES             Location/Qualifiers
     repeat_unit     1..141
                     /label="left AAV-2ITR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(24..39)
                     /label="DK_AAVTitr-Rev"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="sequence: CGGCCTCAGTGAGCGA"
     primer          68..85
                     /label="77827116_EZ_ITR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="sequence: ctttggtcgcccggcctc"
     promoter        169..624
                     /label="hSyn1(S) Promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          625..649
                     /label="DK_Chronos-5"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="sequence: acgcgtggacggtacccttcaggtc"
     misc_feature    657..690
                     /label="loxP"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    701..734
                     /label="loxP2272"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(726..744)
                     /label="MT_AAV_FLEX_FLIP-5"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="sequence: ggcgcgccaaataacttcg"
     primer          732..760
                     /label="DK_TeTx-5"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="sequence: GAAGTTGTTGATGGTGATCGGCATGAATT"
     misc_feature    741..871
                     /label="DK_TeTox-mRuby3-3"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          741..871
                     /label="DK_Tetox-mRuby3-3"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="sequence: CGCCGCTAGCTTTCAGAATTCTGGACCTGGATTGCTTTCTACATCCCCAGCCAGTTTGAGTAAATCAAAGTTAAGAGTTTGTTTGACAGGAGCGACAATTTTGGATCCCTTATAAAGTTCATCCATACCAC"
     CDS             complement(751..848)
                     /label="Translation 848-751"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="GSKIVAPVKQTLNFDLLKLAGDVESNPGPEF*"
     misc_feature    756..871
                     /label="DK_TeTox-mRuby3-1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(762..848)
                     /label="2A?"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          814..871
                     /label="TRC_mRuby_rev"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="sequence: cgtatagcatacattatacgaagttatggcgcgccCTTATAAAGTTCATCCATACCAC"
     primer          complement(827..848)
                     /label="DK_TeTx-8"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="sequence: GGATCCAAAATTGTCGCTCCTG"
     misc_feature    849..871
                     /label="Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(849..1559)
                     /label="mRuby3"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(849..1559)
                     /label="Translation 1559-849"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMRIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGVVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDIALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVAKYSNLGGGMDELYK"
     misc_feature    complement(1539..1559)
                     /label="Priming site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(1539..1559)
                     /label="DK_mRuby3-3"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="sequence: ATGGTGTCTAAGGGCGAAGAG"
     misc_feature    complement(1539..1688)
                     /label="DK_TeTox-mRuby3-2"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1572..1589
                     /label="77775746_EZ_HDR-HA-IFG1_27"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="sequence: ccaacctggccggccttg"
     primer          1580..1598
                     /label="MT_AAV_FLEX_FLIP-6"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="sequence: gccggccttgataacttcg"
     misc_feature    complement(1590..1623)
                     /label="loxP"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(1634..1667)
                     /label="loxP2272"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1693..2280
                     /label="WPRE"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1726..1749)
                     /label="IS_pFUW-4"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="sequence: GCAACATAGTTAAGAATACCAGTC"
     primer          complement(1738..1764)
                     /label="DK_Chronos_9 74402801"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="sequence: catagcgtaaaaggagcaacatagtta"
     primer          1803..1824
                     /label="75794530_AAVTitr_WPRE-fwd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="sequence: CTTCCCGTATGGCTTTCATTTT"
     primer          2079..2098
                     /label="DK_TeTX-11"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="sequence: CGGGGAAATCATCGTCCTTT"
     misc_feature    complement(2314..2331)
                     /label="BGH_rev_primer"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(2314..2331)
                     /label="IS_BGH_reverse_primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="sequence: tagaaggcacagtcgagg"
     terminator      2317..2520
                     /label="bGH_PA_terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     repeat_unit     2550..2690
                     /label="right AAV-2ITR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2568..2587
                     /label="DK_Tetx-12"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: AGTTGGCCACTCCCTCTCTG"
     rep_origin      2782..3088
                     /label="f1_origin"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(3000..3019)
                     /label="DK_Tetx-15"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="sequence: TCTATCAGGGCGATGGCCCA"
     primer          3074..3093
                     /label="DK_Tetx-13"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="sequence: TCCAAACTGGAACAACACTC"
     misc_feature    3356..3378
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(3486..3505)
                     /label="DK_Tetx-14"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="sequence: CGCGCACATTTCCCCGAAAA"
     promoter        3537..3565
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     gene            3607..4467
                     /label="Ampicillin"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     rep_origin      4622..5241
                     /label="pBR322_origin"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTCTGCA GAGGGCCCTG CGTATGAGTG
      181 CAAGTGGGTT TTAGGACCAG GATGAGGCGG GGTGGGGGTG CCTACCTGAC GACCGACCCC
      241 GACCCACTGG ACAAGCACCC AACCCCCATT CCCCAAATTG CGCATCCCCT ATCAGAGAGG
      301 GGGAGGGGAA ACAGGATGCG GCGAGGCGCG TGCGCACTGC CAGCTTCAGC ACCGCGGACA
      361 GTGCCTTCGC CCCCGCCTGG CGGCGCGCGC CACCGCCGCC TCAGCACTGA AGGCGCGCTG
      421 ACGTCACTCG CCGGTCCCCC GCAAACTCCC CTTCCCGGCC ACCTTGGTCG CGTCCGCGCC
      481 GCCGCCGGCC CAGCCGGACC GCACCACGCG AGGCGCGAGA TAGGGGGGCA CGGGCGCGAC
      541 CATCTGCGCT GCGGCGCCGG CGACTCAGCG CTGCCTCAGT CTGCGGTGGG CAGCGGAGGA
      601 GTCGTGTCGT GCCTGAGAGC GCAGACGCGT GGACGGTACC CTTCAGGTCG ACGTCGATAA
      661 CTTCGTATAG CATACATTAT ACGAAGTTAT CCGAATCGCA ATAACTTCGT ATAGGATACT
      721 TTATACGAAG TTATTTGGCG CGCCGCTAGC TTTCAGAATT CTGGACCTGG ATTGCTTTCT
      781 ACATCCCCAG CCAGTTTGAG TAAATCAAAG TTAAGAGTTT GTTTGACAGG AGCGACAATT
      841 TTGGATCCCT TATAAAGTTC ATCCATACCA CCACCAAGAT TGGAGTACTT GGCAACTGCG
      901 ACTTCGCGTT GTACTACGTA CGTTTCATTG TCACTTTCCT CTATCCTTTC CAGGCGGTGA
      961 TCAACGGCAT GGACACCGGG CATCTTGATG TTCCCGACGG TCTTTTTTGA CCTGTAAGTT
     1021 GTTACGAAAT TGCAATGCAG ATGGCCACCA CCATCAACTT TCAGTGCAAT ATCAGTGTAT
     1081 CCCCTCAGAC CACCATCTGC TGGATACATC ATCTCTGTAT TAGGCTCCCA ACCCTTGGTC
     1141 TTCTTCTGCA TCACGGGACC ATTGGAGGGA AAGTTTACCC CTCTGACTTT GACGTTGTAA
     1201 ACGAGTTCGC CATCCTCAAG GCTGGTGTCC TGCGTGACGG TGACGACTCC ACCATCTTCG
     1261 TATCTCGTAA CTCTTTCCCA AGTAAAACCC TCAGGAAAGG ACTGTTTAAA GAAATCAGGA
     1321 ATGTCTGCCG GGTACTTGAT AAAAGTACGG CTGCCATACA TGAACGACGT GGCAAGAATG
     1381 TCAAAGGCAA ATGGCAGGGG TCCTCCCTCG ATGACTTTGA TCCTCATGGT TTGCACTCCC
     1441 TCGTACGGCC TGCCTTCTCC TTCACCTGTG CATTTGAATT GGTGGCCGTT GACCGAACCT
     1501 TCCATGACCA CCTTCATACG CATATTTTCC TTGATCAGCT CTTCGCCCTT AGACACCATG
     1561 GTGGCGGCGC GCCAACCTGG CCGGCCTTGA TAACTTCGTA TAATGTATGC TATACGAAGT
     1621 TATCCGAATC GCAATAACTT CGTATAAAGT ATCCTATACG AAGTTATCGA AGATCATCAA
     1681 GCTTATCGAT AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA
     1741 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT
     1801 TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA
     1861 TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC
     1921 AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT
     1981 CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG
     2041 GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC
     2101 TTGGCTGCTC GCCTATGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC
     2161 TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT
     2221 TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCA
     2281 TCGATACCGA GCGCTGCTCG AGAGATCGAT CTGCCTCGAC TGTGCCTTCT AGTTGCCAGC
     2341 CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG
     2401 TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC
     2461 TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG
     2521 CTGGGGACAC GTGCGGACCG AGCGGCCGCA GGAACCCCTA GTGATGGAGT TGGCCACTCC
     2581 CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA AAGGTCGCCC GACGCCCGGG
     2641 CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC TGCCTGCAGG GGCGCCTGAT
     2701 GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATACGTC AAAGCAACCA
     2761 TAGTACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG
     2821 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC
     2881 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA
     2941 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT TGGGTGATGG TTCACGTAGT
     3001 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT
     3061 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGGCTA TTCTTTTGAT
     3121 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA
     3181 TTTAACGCGA ATTTTAACAA AATATTAACG TTTACAATTT TATGGTGCAC TCTCAGTACA
     3241 ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGACGCG
     3301 CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG
     3361 AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGACG AAAGGGCCTC
     3421 GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT
     3481 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA
     3541 AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG
     3601 AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC
     3661 CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG
     3721 GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT
     3781 CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA
     3841 TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT
     3901 GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA
     3961 GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA
     4021 ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT
     4081 CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC
     4141 ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT
     4201 CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT
     4261 CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT
     4321 GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT
     4381 ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA
     4441 GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG
     4501 ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT
     4561 CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA
     4621 AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA
     4681 AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT
     4741 CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG
     4801 TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC
     4861 CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA
     4921 CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC
     4981 AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC
     5041 GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA
     5101 GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG
     5161 TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA
     5221 TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT
     5281 CACATGT
//