LOCUS       Exported               12878 bp ds-DNA     circular SYN 03-JUN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12878)
  AUTHORS   Columbia University
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 5, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12878
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1679..1912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2439..2556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2613..2883
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2916..2954
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3055..3060
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     6702..6740
                     /label=Primer 1
     CDS             6727..6783
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     6760..6816
                     /label=Primer 1 (2)
     primer_bind     complement(6769..6791)
                     /label=Primer 2 (1)
     CDS             6793..7497
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     regulatory      6793
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(7474..7533)
                     /label=Primer 2 (2)
     primer_bind     7501..7535
                     /label=Primer 1 (1)
     misc_feature    7523..8111
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     7523..7558
                     /label=Primer 1
     primer_bind     complement(7530..7567)
                     /label=Primer 2
     CDS             complement(7994..8005)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8114..8130)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8435..8458
                     /label=Sc ORF BC R
     gap             8464..8473
                     /estimated_length=10
     LTR             8682..8862
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8894..9118
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9164..9592
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9606..9935
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     9781..9793
                     /label=Primer 2 (2)
     rep_origin      9786..9921
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9983..10030
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10049..10423
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10553..10674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10723..10739)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10747..10763
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10771..10801)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10816..10837
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(11125..11713)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11884..12744)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12745..12849)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2581 gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     2701 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     2761 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     2821 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2881 agaaaagtga aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct
     2941 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
     3001 ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa ttccgccacc
     3061 atgaagcagc cgatcatggc ggatggcccc cggtgcaaga ggcgcaaaca agccaatccc
     3121 aggaggaaaa acgtggtgaa ctatgacaat gtagtggaca caggttctga aacagatgag
     3181 gaagacaagc ttcatattgc tgaggatgac ggtattgcca accctctgga ccaggagacg
     3241 agtccagcta gtgtgcccaa ccatgagtcc tccccacacg tgagccaagc tctgttgcca
     3301 agagaggaag aggaagatga aataagggag ggtggagtgg aacacccctg gcacaacaac
     3361 gagattctac aagcctctgt agatggtcca gaagaaatga aggaagacta tgacactatg
     3421 gggccagaag ccacgatcca gaccgcaatt aacaatggta cagtgaagaa tgcaaattgc
     3481 acatcagatt ttgaggaata ctttgccaaa agaaaactgg aggaacgcga tggtcatgca
     3541 gtcagcatcg aggagtacct tcagcgcagt gacacagcca ttatttaccc agaagcccct
     3601 gaggagctgt ctcgccttgg cacgccagag gccaatgggc aagaagaaaa tgacctgcca
     3661 cctggaactc cagatgcttt tgcccaactg ctgacctgcc cctactgcga ccggggctac
     3721 aagcgcttga catcactgaa ggagcacatc aagtaccgcc acgagaagaa tgaagagaac
     3781 ttttcctgcc ctctctgtag ctacacgttt gcctaccgca cccagctcga gcggcatatg
     3841 gtgacacaca agccagggac agatcagcac caaatgctaa cccaaggagc aggtaatcgc
     3901 aagttcaaat gcacagagtg tggcaaggcc ttcaaatata aacaccatct gaaagaacac
     3961 ctgcgaattc acagtggtga aaaaccttac gagtgcccaa actgcaagaa acgtttctcc
     4021 cattctggtt cctacagttc gcacatcagc agcaagaaat gtattggttt aatctctgta
     4081 aatggccgaa tgagaaacaa tatcaagacg ggttcttccc ctaattctgt ttcttcttct
     4141 cctactaatt cagccattac ccagttaaga aacaagttgg agaatggaaa accacttagt
     4201 atgtctgaac agacaggctt acttaaaatt aaaacagaac cactagactt caatgactat
     4261 aaagttctta tggctacaca cgggtttagt ggcactagtc cctttatgaa tggtgggctt
     4321 ggagccacca gccctttagg agttcatcca tctgctcaga gtccaatgca gcacttaggt
     4381 gtagggatgg aagccccttt acttgggttt cccaccatga atagtaattt aagtgaggta
     4441 caaaaggttc tacagattgt ggacaatact gtttccaggc aaaaaatgga ctgcaaggct
     4501 gaagaaattt caaagttgaa aggttatcac atgaaggatc catgctctca acctgaggaa
     4561 caaggagtta cttctcctaa tattccgcct gtcggtcttc cggtagtgag tcataatggt
     4621 gccactaaaa gtattattga ctatacgttg gaaaaagtca atgaagccaa agcttgcctc
     4681 cagagcttga ctactgactc aaggagacag atcagtaata taaagaaaga gaagctacgt
     4741 actttaatag atttggtcac tgatgacaaa atgattgaga accacaacat atccactcca
     4801 ttttcatgcc agttctgtaa agaaagtttt cctggcccca tccctttgca tcagcatgaa
     4861 cgttaccttt gtaagatgaa tgaagagatc aaggcggtcc tgcagcctca tgaaaacata
     4921 gtccccaaca aagccggagt ttttgttgat aataaagccc tcctcttgtc atctgtactt
     4981 tctgagaaag gaatgacaag ccccatcaac ccatacaagg accacatgtc tgtactcaaa
     5041 gcatactatg ctatgaacat ggagcccaac tccgatgaac tgctgaaaat ttccattgct
     5101 gtgggccttc ctcaggaatt tgtgaaggaa tggtttgaac aacgaaaagt ctaccagtac
     5161 tcaaattcca ggtccccatc cctggaaaga agctccaagc cgttagctcc caacagtaac
     5221 cctcccacaa aagactcttt attacccagg tctcctgtaa aacctatgga ctccataaca
     5281 tcaccatcta tagcagaact ccacaacagt gttacgaatt gtgatcctcc tctcaggcta
     5341 acaaaacctt cccattttac caatattaaa ccagttgaaa aattggacca ctccaggagt
     5401 aatactcctt ctcccttaaa tctttcctcc acatcttcta aaaactccca cagtagttca
     5461 tacactccaa acagcttctc ttctgaggag ctccaggctg agcctttaga cttgtcatta
     5521 ccaaaacaaa tgaaagaacc caaaagtatt atagccacaa agaacaaaac aaaagctagt
     5581 agcatcagtt tagatcataa cagtgtttct tcctcatctg aaaactcaga tgagcctctg
     5641 aacttgactt ttatcaagaa ggaattttca aattcaaata atctggacaa caaaagcact
     5701 aacccagtgt tcagcatgaa cccatttagt gccaaacctt tatacacagc tcttccacct
     5761 caaagcgcat ttccccctgc tactttcatg ccaccagtcc agaccagtat tcctgggcta
     5821 cgaccatacc caggactgga tcagatgagc ttcctaccac atatggccta cacctaccca
     5881 actggagcag ctacttttgc tgatatgcag caaaggagaa agtaccagcg gaaacaagga
     5941 tttcagggag aattgcttga tggagcacaa gactacatgt caggcctaga tgatatgaca
     6001 gactccgact cctgtctgtc tcgcaaaaag atcaagaaga cagagagtgg catgtatgca
     6061 tgtgacttat gtgacaagac attccagaaa agcagttccc ttctgcgaca taaatacgaa
     6121 cacacaggaa aaagaccaca tcagtgtcag atttgtaaga aagcgtttaa acacaagcac
     6181 caccttatcg agcactcaag gcttcactcg ggcgagaagc cctatcagtg tgataaatgt
     6241 ggcaagcgct tctcacactc gggctcgtac tcgcagcaca tgaatcacag gtattcctac
     6301 tgcaagcggg aggcggagga gcgggaagcg gcggagcgcg aggcgcgcga gaaagggcac
     6361 ttggaaccca ccgagctgct gatgaaccgg gcttacttgc agagcattac ccctcagggg
     6421 tactctgact cggaggagag ggagagtatg ccgagggatg gcgagagcga gaaggagcac
     6481 gagaaagaag gcgaggatgg ctacgggaag ctgggcagac aggatggcga cgaggagttc
     6541 gaggaggaag aggaagaaag tgaaaataaa agtatggata cggatcccga aacgatacga
     6601 gatgaagaag agactggaga tcactccatg gacgatagtt cggaggatgg gaaaatggaa
     6661 accaaatcag accacgagga agacaatatg gaagatggca tgacgcgtga attcgatgga
     6721 tccggggcca ccaacttttc cctgctgaag caggctggcg acgtcgagga gaaccctggc
     6781 cctggctccg gagtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     6841 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     6901 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     6961 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     7021 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     7081 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     7141 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     7201 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     7261 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     7321 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     7381 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     7441 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtga
     7501 atctagaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     7561 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     7621 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     7681 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     7741 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     7801 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7861 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     7921 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7981 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     8041 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     8101 cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag
     8161 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     8221 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     8281 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     8341 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     8401 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaaggc
     8461 tagnnnnnnn nnnggaattc gcccgaacgg gaaagctagc agaactagta ccagttgagc
     8521 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
     8581 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
     8641 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
     8701 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8761 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8821 gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc
     8881 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     8941 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     9001 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     9061 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     9121 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     9181 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     9241 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     9301 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     9361 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     9421 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     9481 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     9541 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     9601 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     9661 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     9721 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     9781 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     9841 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     9901 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     9961 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
    10021 ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct
    10081 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
    10141 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
    10201 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
    10261 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
    10321 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
    10381 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
    10441 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
    10501 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
    10561 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    10621 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10681 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
    10741 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    10801 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    10861 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    10921 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    10981 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    11041 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    11101 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    11161 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    11221 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    11281 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    11341 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    11401 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    11461 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    11521 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    11581 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    11641 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    11701 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    11761 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    11821 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    11881 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    11941 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    12001 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    12061 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    12121 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    12181 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    12241 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    12301 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    12361 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    12421 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    12481 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    12541 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    12601 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    12661 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    12721 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    12781 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    12841 ggttccgcgc acatttcccc gaaaagtgcc acctgacg
//
