LOCUS       Exported               11030 bp ds-DNA     circular SYN 03-JUN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11030)
  AUTHORS   Columbia University
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 5, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11030
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1679..1912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2439..2556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2613..2883
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2916..2954
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3055..3060
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     4836..4892
                     /label=Primer 1
     CDS             4879..4935
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     4912..4968
                     /label=Primer 1 (2)
     primer_bind     complement(4921..4943)
                     /label=Primer 2 (1)
     CDS             4945..5649
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     regulatory      4945
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(5626..5685)
                     /label=Primer 2 (2)
     primer_bind     5653..5687
                     /label=Primer 1 (1)
     misc_feature    5675..6263
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     5675..5710
                     /label=Primer 1
     primer_bind     complement(5682..5719)
                     /label=Primer 2
     CDS             complement(6146..6157)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6266..6282)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6587..6610
                     /label=Sc ORF BC R
     gap             6616..6625
                     /estimated_length=10
     LTR             6834..7014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7046..7270
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7316..7744
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7758..8087
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     7933..7945
                     /label=Primer 2 (2)
     rep_origin      7938..8073
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8135..8182
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8201..8575
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8705..8826
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8875..8891)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8899..8915
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8923..8953)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8968..8989
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9277..9865)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10036..10896)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10897..11001)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2581 gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     2701 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     2761 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     2821 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2881 agaaaagtga aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct
     2941 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
     3001 ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa ttccgccacc
     3061 atgccctgta tccaagccca atatgggaca ccagcaccga gtccgggacc ccgtgaccac
     3121 ctggcaagcg accccctgac ccctgagttc atcaagccca ccatggacct ggccagcccc
     3181 gaggcagccc ccgctgcccc cactgccctg cccagcttca gcaccttcat ggacggctac
     3241 acaggagagt ttgacacctt cctctaccag ctgccaggaa cagtccagcc atgctcctca
     3301 gcctcctcct cggcctcctc cacatcctcg tcctcagcca cctcccctgc ctctgcctcc
     3361 ttcaagttcg aggacttcca ggtgtacggc tgctaccccg gccccctgag cggcccagtg
     3421 gatgaggccc tgtcctccag tggctctgac tactatggca gcccctgctc ggccccgtcg
     3481 ccctccacgc ccagcttcca gccgccccag ctctctccct gggatggctc cttcggccac
     3541 ttctcgccca gccagactta cgaaggcctg cgggcatgga cagagcagct gcccaaagcc
     3601 tctgggcccc cacagcctcc agccttcttt tccttcagtc ctcccaccgg ccccagcccc
     3661 agcctggccc agagccccct gaagttgttc ccctcacagg ccacccacca gctgggggag
     3721 ggagagagct attccatgcc tacggccttc ccaggtttgg cacccacttc tccacacctt
     3781 gagggctcgg ggatactgga tacacccgtg acctcaacca aggcccggag cggggcccca
     3841 ggtggaagtg aaggccgctg tgctgtgtgt ggggacaacg cttcatgcca gcattatggt
     3901 gtccgcacat gtgagggctg caagggcttc ttcaagcgca cagtgcagaa aaacgccaag
     3961 tacatctgcc tggctaacaa ggactgccct gtggacaaga ggcggcgaaa ccgctgccag
     4021 ttctgccgct tccagaagtg cctggcggtg ggcatggtga aggaagttgt ccgaacagac
     4081 agcctgaagg ggcggcgggg ccggctacct tcaaaaccca agcagccccc agatgcctcc
     4141 cctgccaatc tcctcacttc cctggtccgt gcacacctgg actcagggcc cagcactgcc
     4201 aaactggact actccaagtt ccaggagctg gtgctgcccc actttgggaa ggaagatgct
     4261 ggggatgtac agcagttcta cgacctgctc tccggttctc tggaggtcat ccgcaagtgg
     4321 gcggagaaga tccctggctt tgctgagctg tcaccggctg accaggacct gttgctggag
     4381 tcggccttcc tggagctctt catcctccgc ctggcgtaca ggtctaagcc aggcgagggc
     4441 aagctcatct tctgctcagg cctggtgcta caccggctgc agtgtgcccg tggcttcggg
     4501 gactggattg acagtatcct ggccttctca aggtccctgc acagcttgct tgtcgatgtc
     4561 cctgccttcg cctgcctctc tgcccttgtc ctcatcaccg accggcatgg gctgcaggag
     4621 ccgcggcggg tggaggagct gcagaaccgc atcgccagct gcctgaagga gcacgtggca
     4681 gctgtggcgg gcgagcccca gccagccagc tgcctgtcac gtctgttggg caaactgccc
     4741 gagctgcgga ccctgtgcac ccagggcctg cagcgcatct tctacctcaa gctggaggac
     4801 ttggtgcccc ctccacccat cattgacaag atcttcatgg acacgctgcc cttcacgcgt
     4861 gaattcgatg gatccggggc caccaacttt tccctgctga agcaggctgg cgacgtcgag
     4921 gagaaccctg gccctggctc cggagtgagc aagggcgagg aggataacat ggccatcatc
     4981 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     5041 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     5101 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     5161 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     5221 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     5281 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     5341 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     5401 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     5461 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     5521 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     5581 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     5641 ctgtacaagt gaatctagaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg
     5701 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     5761 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     5821 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     5881 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     5941 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     6001 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     6061 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     6121 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     6181 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     6241 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg
     6301 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     6361 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     6421 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     6481 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     6541 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     6601 gtgctacaag gctagnnnnn nnnnncacgg ttgcgcgtcc gtatcaacta gcagaactag
     6661 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     6721 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     6781 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     6841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     7021 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     7081 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     7141 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     7201 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     7261 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     7321 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     7381 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7441 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7501 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     7561 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7621 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     7681 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7741 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     7801 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     7861 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     7921 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7981 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     8041 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     8101 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     8161 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     8221 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     8281 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     8341 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     8401 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     8461 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     8521 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     8581 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8641 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8701 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8761 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8821 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     8881 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8941 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     9001 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9061 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9121 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9181 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9241 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9301 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9361 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9421 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9481 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9541 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9601 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9661 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     9721 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9781 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9841 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9901 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9961 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10021 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10081 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10141 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10201 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10261 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10321 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10381 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10441 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10501 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10561 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10621 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10681 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10741 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10801 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10861 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10921 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10981 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
//
