LOCUS       pHflu3                  6124 bp    DNA     circular UNA 05-JUN-2023
DEFINITION  pSU20-CAH-oriT-kan.
ACCESSION   urn.local...1w-f9qas67
VERSION     urn.local...1w-f9qas67
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     rep_origin      1..845
                     /note="p15A origin of replication"
                     /modified_by="Samir"
                     /modified_by="Samir"
                     /label="p15A ori"
     gene            846..1075
                     /note="Lac-operon"
                     /modified_by="Samir"
                     /label="lacZ'"
     Cloning_Site    join(1078..>1102,<2480..2498)
                     /created_by="Samir"
                     /modified_by="Samir"
                     /modified_by="Samir"
                     /label="MCS"
     CDS             complement(1301..2095)
                     /Genetic_code="Standard"
                     /Reading_frame=1
                     /created_by="ORF Finder"
                     /modified_by="Samir"
                     /label="nptII"
     gene            complement(1301..2095)
                     /created_by="Samir"
                     /modified_by="Samir"
                     /label="kanR"
     gene            2499..2656
                     /note="Lac-Operon"
                     /modified_by="Samir"
                     /modified_by="Samir"
                     /label="lacZ'"
     Synthetic       2778..5208
                     /created_by="hama-"
                     /Color="#ffff00"
                     /modified_by="hama-"
                     /label="HIS3-CEN6-ARS4-oriT"
     promoter        2906..3067
                     /created_by="Samir"
                     /Transferred_From="cen ars his cassette"
                     /Transferred_Similarity="99.38%"
                     /modified_by="Samir"
                     /label="his3 promoter"
     gene            3094..3756
                     /created_by="Samir"
                     /Transferred_From="cen ars his oriT"
                     /Transferred_Similarity="100.00%"
                     /modified_by="Samir"
                     /label="HIS3"
     Centromere      3917..4034
                     /created_by="Samir"
                     /Transferred_From="cen6"
                     /Transferred_Similarity="100.00%"
                     /modified_by="Samir"
                     /label="CEN6"
     rep_origin      4198..4430
                     /created_by="Samir"
                     /Transferred_From="ars209"
                     /Transferred_Similarity="98.71%"
                     /modified_by="Samir"
                     /label="ARSH4"
     misc_feature    4566..4677
                     /created_by="Samir"
                     /modified_by="Samir"
                     /label="oriT"
                     /note="Geneious type: Origin of transfer"
     misc_feature    4710..5081
                     /created_by="Samir"
                     /Transferred_From="pTA-MOB"
                     /Transferred_Similarity="99.73%"
                     /Color="#973bff"
                     /label="traJ"
     misc_feature    5078..5119
                     /gene="traX"
                     /operon="traJXIH"
                     /function="translational coupling"
                     /codon_start=1
                     /transl_table=11
                     /product="TraX"
                     /protein_id="CAJ85727.1"
                     /db_xref="UniProtKB/TrEMBL:A6H979"
                     /Transferred_Translation="MTFLAAKTAGGCA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /modified_by="hama-"
                     /Color="#973bff"
                     /label="traX"
     CDS             5441..6100
                     /gene="cat"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA23899.1"
                     /db_xref="GOA:P62577"
                     /db_xref="InterPro:IPR001707"
                     /db_xref="InterPro:IPR018372"
                     /db_xref="PDB:1NOC"
                     /db_xref="PDB:1PD5"
                     /db_xref="PDB:1Q23"
                     /db_xref="UniProtKB/Swiss-Prot:P62577"
                     /Transferred_Translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCT
                     YNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHP
                     CYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSAN
                     PWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLN
                     ELQQYCDEWQGGA"
                     /note="Derived using Geneious Prime 2020.2.3 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="Tn9"
                     /Transferred_Similarity="99.85%"
                     /Primary_Match="99.85%; Adjusted-Interval-0:0/0; cat CDS
                     (Tn9)"
                     /note="codon 73 mutated (TTC>TTT) to eliminate EcoRI site
                     (Takeshita et al 1987)"
                     /modified_by="Samir"
     gene            5441..6100
                     /gene="cat"
                     /note="Derived using Geneious Prime 2020.2.3 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="Tn9"
                     /Transferred_Similarity="99.85%"
                     /Primary_Match="99.85%; Adjusted-Interval-0:0/0; cat gene
                     (Tn9)"
                     /modified_by="Samir"
                     /label="cmR"
ORIGIN      
        1 ctagaaatat tttatctgat taataagatg atcttcttga gatcgttttg gtctgcgcgt
       61 aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg
      121 agctaccaac tctttgaacc gaggtaactg gcttggagga gcgcagtcac caaaacttgt
      181 cctttcagtt tagccttaac cggcgcatga cttcaagact aactcctcta aatcaattac
      241 cagtggctgc tgccagtggt gcttttgcat gtctttccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg
      361 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac
      421 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca
      481 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt
      541 cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt tgccgcggcc
      601 ctctcacttc cctgttaagt atcttcctgg catcttccag gaaatctccg ccccgttcgt
      661 aagccatttc cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag
      721 cggaatatat cctgtatcac atattctgct gacgcaccgg tgcagccttt tttctcctgc
      781 cacatgaagc acttcactga caccctcatc agtgccaaca tagtaagcca gtatacactc
      841 cgctagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
      901 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
      961 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     1021 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     1081 cttgcatgcc cctcgaggtc gagcgacagg gcgaagcccg tttaaacggg gtgggcgaag
     1141 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt
     1201 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg
     1261 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
     1321 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     1381 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     1441 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     1501 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     1561 gcatccgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     1621 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     1681 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     1741 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     1801 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     1861 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttggagtt
     1921 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     1981 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     2041 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
     2101 acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg
     2161 gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag
     2221 ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa
     2281 gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc cagatagccc
     2341 agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc tacgtgttcc
     2401 gcttccttta gcagcccttg cgccctgagt gcttgcggca gcgtgaagct ttaattaaat
     2461 cttccgctgc ataaccctgg ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt
     2521 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     2581 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     2641 ctgaatggcg aatggcgctg atgtccggcg gtgcttttgc cgttacgcac caccccgtca
     2701 gtagctgaac aggagggaca gctgatagaa acagaagcca ctggagcacc tcaaaaacac
     2761 catcatacac taaatcatta attaataaga gcttggtgag cgctaggagt cactgccagg
     2821 tatcgtttga acacggcatt agtcagggaa gtcataacac agtcctttcc cgcaattttc
     2881 tttttctatt actcttggcc tcctctagta cactctatat ttttttatgc ctcggtaatg
     2941 attttcattt tttttttttc cacctagcgg atgactcttt ttttttctta gcgattggca
     3001 ttatcacata atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta
     3061 aaaaatgagc aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag
     3121 cgtattacaa atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg
     3181 atagagcact cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa
     3241 tcgcaagtga ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg
     3301 gccaagcatt ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac
     3361 catcacacca ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctaggg
     3421 gccgtgcgtg gagtaaaaag gtttggatca ggatttgcgc ctttggatga ggcactttcc
     3481 agagcggtgg tagatctttc gaacaggccg tacgcagttg tcgaacttgg tttgcaaagg
     3541 gagaaagtag gagatctctc ttgcgagatg atcccgcatt ttcttgaaag ctttgcagag
     3601 gctagcagaa ttaccctcca cgttgattgt ctgcgaggca agaatgatca tcaccgtagt
     3661 gagagtgcgt tcaaggctct tgcggttgcc ataagagaag ccacctcgcc caatggtacc
     3721 aacgatgttc cctccaccaa aggtgttctt atgtagtgac accgattatt taaagctgca
     3781 gcatacgata tatatacatg tgtatatatg tatacctatg aatgtcagta agtatgtata
     3841 cgaacagtat gatactgaag atgacaaggt aatgcatcat tctatacgtg tcattctgaa
     3901 cgaggcgcgc tttcctatca cgtgctataa aaataattat aatttaaatt ttttaatata
     3961 aatatataaa ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt
     4021 tccgaagatg taaatctaga gactctaggg ggatcgccaa caaatactac cttttatctt
     4081 gctcttcctg ctctcaggta ttaatgccga attgtttcat cttgtctgtg tagaagacca
     4141 cacacgaaaa tcctgtgatt ttacatttta cttatcgtta atcgaatgta tatctattta
     4201 atctgctttt cttgtctaat aaatatatat gtaaagtacg ctttttgttg aaatttttta
     4261 aacctttgtt tatttttttt tcttcattcc gtaactcttc taccttcttt atttactttc
     4321 taaaatccaa atacaaaaca taaaaataaa taaacacaga gtaaattccc aaattattcc
     4381 atcattaaaa gatacgaggc gcgtgtaagt tacaggcaag cgatccacta gtatcttccg
     4441 ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg
     4501 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc
     4561 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
     4621 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta
     4681 ccgccggcgt aacagatgag ggcaagcgga tggctgatga aaccaagcca accaggaagg
     4741 gcagcccacc tatcaaggtg tactgccttc cagacgaacg aagagcgatt gaggaaaagg
     4801 cggcggcggc cggcatgagc ctgtcggcct acctgctggc cgtcggccag ggctacaaaa
     4861 tcacgggcgt cgtggactat gagcacgtcc gcgagctggc ccgcatcaat ggcgacctgg
     4921 gccgcctggg cggcctgctg aaactctggc tcaccgacga cccgcgcacg gcgcggttcg
     4981 gtgatgccac gatcctcgcc ctgctggcga agatcgaaga gaagcaggac gagcttggca
     5041 aggtcatgat gggcgtggtc cgcccgaggg cagagccatg acttttttag ccgctaaaac
     5101 ggccgggggg tgcgcgtgat tgccaagcac gtccccatgc gctccatcaa gaagaggcac
     5161 ttcgagctgt aagtacatca ccgacgagca aggcaagacg atcgagctag catcacccga
     5221 cgcactttgc gccgaataaa tacctgtgac ggaagatcac ttcgcagaat aaataaatcc
     5281 tggtgtccct gttgataccg ggaagccctg ggccaacttt tggcgaaaat gagacgttga
     5341 tcggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
     5401 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     5461 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     5521 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     5581 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     5641 gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag
     5701 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     5761 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     5821 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     5881 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     5941 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     6001 ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga
     6061 attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg
     6121 gtgc
//
