LOCUS       pHflu4                  5660 bp    DNA     circular UNA 05-JUN-2023
DEFINITION  pSU20-CAH-oriT-mRFP.
ACCESSION   urn.local...2x-gcr1tdr
VERSION     urn.local...2x-gcr1tdr
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     rep_origin      1..845
                     /note="p15A origin of replication"
                     /modified_by="Samir"
                     /label="p15A ori"
     gene            846..1075
                     /note="Lac-operon"
                     /modified_by="Samir"
                     /label="lacZ'"
     Cloning_Site    join(1078..>1098,<2016..2034)
                     /created_by="Samir"
                     /modified_by="Samir"
                     /label="MCS"
     promoter        1099..1133
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /modified_by="Samir"
                     /label="pAnderson"
     regulatory      1155..1166
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /modified_by="Samir"
                     /label="RBS"
     CDS             1173..1850
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /modified_by="Samir"
                     /label="mRFP"
     gene            1173..1850
                     /created_by="Samir"
                     /label="mRFP"
     terminator      1887..2015
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /modified_by="Samir"
                     /label="Double Terminator"
     gene            2035..2192
                     /note="Lac-Operon"
                     /modified_by="Samir"
                     /label="lacZ'"
     Synthetic       2314..4744
                     /created_by="hama-"
                     /Color="#ffff00"
                     /modified_by="hama-"
                     /label="HIS3-CEN6-ARS4-oriT"
     promoter        2442..2603
                     /created_by="Samir"
                     /Transferred_From="cen ars his cassette"
                     /Transferred_Similarity="99.38%"
                     /modified_by="Samir"
                     /label="his3 promoter"
     gene            2630..3292
                     /created_by="Samir"
                     /Transferred_From="cen ars his oriT"
                     /Transferred_Similarity="100.00%"
                     /modified_by="Samir"
                     /label="HIS3"
     Centromere      3453..3570
                     /created_by="Samir"
                     /Transferred_From="cen6"
                     /Transferred_Similarity="100.00%"
                     /modified_by="Samir"
                     /label="CEN6"
     rep_origin      3734..3966
                     /created_by="Samir"
                     /Transferred_From="ars209"
                     /Transferred_Similarity="98.71%"
                     /modified_by="Samir"
                     /label="ARSH4"
     misc_feature    4102..4213
                     /created_by="Samir"
                     /modified_by="Samir"
                     /label="oriT"
                     /note="Geneious type: Origin of transfer"
     misc_feature    4246..4617
                     /created_by="Samir"
                     /Transferred_From="pTA-MOB"
                     /Transferred_Similarity="99.73%"
                     /Color="#973bff"
                     /label="traJ"
     misc_feature    4614..4655
                     /gene="traX"
                     /operon="traJXIH"
                     /function="translational coupling"
                     /codon_start=1
                     /transl_table=11
                     /product="TraX"
                     /protein_id="CAJ85727.1"
                     /db_xref="UniProtKB/TrEMBL:A6H979"
                     /Transferred_Translation="MTFLAAKTAGGCA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /modified_by="hama-"
                     /Color="#973bff"
                     /label="traX"
     CDS             4977..5636
                     /gene="cat"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA23899.1"
                     /db_xref="GOA:P62577"
                     /db_xref="InterPro:IPR001707"
                     /db_xref="InterPro:IPR018372"
                     /db_xref="PDB:1NOC"
                     /db_xref="PDB:1PD5"
                     /db_xref="PDB:1Q23"
                     /db_xref="UniProtKB/Swiss-Prot:P62577"
                     /Transferred_Translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCT
                     YNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHP
                     CYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSAN
                     PWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLN
                     ELQQYCDEWQGGA"
                     /note="Derived using Geneious Prime 2020.2.3 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="Tn9"
                     /Transferred_Similarity="99.85%"
                     /Primary_Match="99.85%; Adjusted-Interval-0:0/0; cat CDS
                     (Tn9)"
                     /note="codon 73 mutated (TTC>TTT) to eliminate EcoRI site
                     (Takeshita et al 1987)"
                     /modified_by="Samir"
                     /label="cat CDS"
     gene            4977..5636
                     /gene="cat"
                     /note="Derived using Geneious Prime 2020.2.3 'Transfer
                     Annotations' based on nucleotide similarity"
                     /Transferred_From="Tn9"
                     /Transferred_Similarity="99.85%"
                     /Primary_Match="99.85%; Adjusted-Interval-0:0/0; cat gene
                     (Tn9)"
                     /modified_by="Samir"
                     /label="cmR"
ORIGIN      
        1 ctagaaatat tttatctgat taataagatg atcttcttga gatcgttttg gtctgcgcgt
       61 aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg
      121 agctaccaac tctttgaacc gaggtaactg gcttggagga gcgcagtcac caaaacttgt
      181 cctttcagtt tagccttaac cggcgcatga cttcaagact aactcctcta aatcaattac
      241 cagtggctgc tgccagtggt gcttttgcat gtctttccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg
      361 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac
      421 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca
      481 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt
      541 cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt tgccgcggcc
      601 ctctcacttc cctgttaagt atcttcctgg catcttccag gaaatctccg ccccgttcgt
      661 aagccatttc cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag
      721 cggaatatat cctgtatcac atattctgct gacgcaccgg tgcagccttt tttctcctgc
      781 cacatgaagc acttcactga caccctcatc agtgccaaca tagtaagcca gtatacactc
      841 cgctagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
      901 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
      961 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     1021 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     1081 cttgcatgcc cctcgaggtt gacagctagc tcagtcctag gtataatgct agctcacaga
     1141 attccatact agagaaagag gagaaatact agatggcttc ctccgaagac gttatcaaag
     1201 agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg
     1261 aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca
     1321 aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca
     1381 aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag
     1441 gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg
     1501 actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc
     1561 cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta
     1621 tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg
     1681 gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc
     1741 cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca
     1801 tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat
     1861 agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1921 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1981 tggctcacct tcgggtgggc ctttctgcgt ttatagggta ccgagctcga attcactggc
     2041 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     2101 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     2161 ccaacagttg cgcagcctga atggcgaatg gcgctgatgt ccggcggtgc ttttgccgtt
     2221 acgcaccacc ccgtcagtag ctgaacagga gggacagctg atagaaacag aagccactgg
     2281 agcacctcaa aaacaccatc atacactaaa tcattaatta ataagagctt ggtgagcgct
     2341 aggagtcact gccaggtatc gtttgaacac ggcattagtc agggaagtca taacacagtc
     2401 ctttcccgca attttctttt tctattactc ttggcctcct ctagtacact ctatattttt
     2461 ttatgcctcg gtaatgattt tcattttttt tttttccacc tagcggatga ctcttttttt
     2521 ttcttagcga ttggcattat cacataatga attatacatt atataaagta atgtgatttc
     2581 ttcgaagaat atactaaaaa atgagcaggc aagataaacg aaggcaaaga tgacagagca
     2641 gaaagcccta gtaaagcgta ttacaaatga aaccaagatt cagattgcga tctctttaaa
     2701 gggtggtccc ctagcgatag agcactcgat cttcccagaa aaagaggcag aagcagtagc
     2761 agaacaggcc acacaatcgc aagtgattaa cgtccacaca ggtatagggt ttctggacca
     2821 tatgatacat gctctggcca agcattccgg ctggtcgcta atcgttgagt gcattggtga
     2881 cttacacata gacgaccatc acaccactga agactgcggg attgctctcg gtcaagcttt
     2941 taaagaggcc ctaggggccg tgcgtggagt aaaaaggttt ggatcaggat ttgcgccttt
     3001 ggatgaggca ctttccagag cggtggtaga tctttcgaac aggccgtacg cagttgtcga
     3061 acttggtttg caaagggaga aagtaggaga tctctcttgc gagatgatcc cgcattttct
     3121 tgaaagcttt gcagaggcta gcagaattac cctccacgtt gattgtctgc gaggcaagaa
     3181 tgatcatcac cgtagtgaga gtgcgttcaa ggctcttgcg gttgccataa gagaagccac
     3241 ctcgcccaat ggtaccaacg atgttccctc caccaaaggt gttcttatgt agtgacaccg
     3301 attatttaaa gctgcagcat acgatatata tacatgtgta tatatgtata cctatgaatg
     3361 tcagtaagta tgtatacgaa cagtatgata ctgaagatga caaggtaatg catcattcta
     3421 tacgtgtcat tctgaacgag gcgcgctttc ctatcacgtg ctataaaaat aattataatt
     3481 taaatttttt aatataaata tataaattaa aaatagaaag taaaaaaaga aattaaagaa
     3541 aaaatagttt ttgttttccg aagatgtaaa tctagagact ctagggggat cgccaacaaa
     3601 tactaccttt tatcttgctc ttcctgctct caggtattaa tgccgaattg tttcatcttg
     3661 tctgtgtaga agaccacaca cgaaaatcct gtgattttac attttactta tcgttaatcg
     3721 aatgtatatc tatttaatct gcttttcttg tctaataaat atatatgtaa agtacgcttt
     3781 ttgttgaaat tttttaaacc tttgtttatt tttttttctt cattccgtaa ctcttctacc
     3841 ttctttattt actttctaaa atccaaatac aaaacataaa aataaataaa cacagagtaa
     3901 attcccaaat tattccatca ttaaaagata cgaggcgcgt gtaagttaca ggcaagcgat
     3961 ccactagtat cttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta
     4021 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct
     4081 tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg
     4141 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg
     4201 cgaggctggc cggctaccgc cggcgtaaca gatgagggca agcggatggc tgatgaaacc
     4261 aagccaacca ggaagggcag cccacctatc aaggtgtact gccttccaga cgaacgaaga
     4321 gcgattgagg aaaaggcggc ggcggccggc atgagcctgt cggcctacct gctggccgtc
     4381 ggccagggct acaaaatcac gggcgtcgtg gactatgagc acgtccgcga gctggcccgc
     4441 atcaatggcg acctgggccg cctgggcggc ctgctgaaac tctggctcac cgacgacccg
     4501 cgcacggcgc ggttcggtga tgccacgatc ctcgccctgc tggcgaagat cgaagagaag
     4561 caggacgagc ttggcaaggt catgatgggc gtggtccgcc cgagggcaga gccatgactt
     4621 ttttagccgc taaaacggcc ggggggtgcg cgtgattgcc aagcacgtcc ccatgcgctc
     4681 catcaagaag aggcacttcg agctgtaagt acatcaccga cgagcaaggc aagacgatcg
     4741 agctagcatc acccgacgca ctttgcgccg aataaatacc tgtgacggaa gatcacttcg
     4801 cagaataaat aaatcctggt gtccctgttg ataccgggaa gccctgggcc aacttttggc
     4861 gaaaatgaga cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat
     4921 cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg
     4981 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     5041 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     5101 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     5161 ttcttgcccg cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc
     5221 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     5281 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     5341 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     5401 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     5461 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     5521 aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc ttccatgtcg
     5581 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt
     5641 tttaaggcag ttattggtgc
//
