LOCUS       Exported                3613 bp ds-DNA     circular UNA 03-MAR-2009
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 5, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     CDS             215..1225
                     /codon_start=1
                     /label=Sm/Sp no DraIII
                     /note="/vntifkey=4"
                     /note="color: #993366"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     variation       1192
                     /label=G to A
                     /label=nonstandard type:  mutation
                     /note="/vntifkey=62"
                     /note="color: #a6acb3"
     misc_feature    1385..1999
                     /label=rep (pMB1)
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     rep_origin      complement(1984)
                     /direction=LEFT
                     /label=ORI
                     /note="RNaseH cleavage point"
                     /note="/vntifkey=33"
                     /note="color: #ffff00"
     CDS             2233..3597
                     /codon_start=1
                     /label=CrGLK
                     /note="color: #993366"
                     /translation="MLAVSPLRSNNNERKKEICEISSSTSFPVVDDFPDFGGANLLDSI
                     DFDELFVGINGEDDVLPDLEMDPHDILAEFSLTESLSPSVSLDHQHHEHSQITSSISAA
                     DYDKLVELEDKANSSSASDDVGSGSSTLTQGEDVVSKSTVLNPSSKEGDKGRKSSSTQS
                     KNSSGKKKVKVDWTPELHRRFVQAVEQLGVDKAVPSRILELMGIDCLTRHNIASHLQKY
                     RSHRKHLLAREAEAANWSQRRQLYGGTAAAAGGGKRDVNPWLAPTMGFPPPMAPAAVPH
                     FRPLHVWGHPSVDQSLMHVWPKHLAPPPRPPTWPPTPTPTPTPPPPPPDPNSFWHSPHQ
                     RVPNPLTPGTPCFPPPLTPARFPTPPVPGIPPPPMYKVDPVAGMGVPAPATGQAVPHAP
                     PLDFHPSTESVDAAIGDVLSKPWLPLPLGLKPPSIDSVLVELQRQGIPKIPPVCT"
     variation       2706
                     /label=Mutation T>A
                     /label=nonstandard type:  mutation
                     /note="For MoClo domestication"
                     /note="color: #a6acb3"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aaatgcttgc tgtgtcacca ttgaggagca ataataatga gagaaaaaag
     2281 gagatatgtg aaattagtag tagtactagt tttccagttg ttgatgattt tcctgatttt
     2341 gggggagcaa atttgcttga tagcattgat tttgatgaac tttttgttgg gattaacggt
     2401 gaggatgatg ttttaccaga tttggaaatg gatcctcatg atattcttgc tgaattctca
     2461 cttactgaat cattatcacc atctgtttct cttgatcatc aacatcatga acattctcaa
     2521 attacctcct caatttcggc ggctgattat gataaactag tagaattaga ggataaggct
     2581 aattcatcat ccgcttcaga cgacgtcggt tcaggctcat ctactttaac tcaaggagaa
     2641 gatgttgtta gcaaatcaac ggttttaaat ccatcttcta aagaaggtga taaaggaagg
     2701 aagtcatctt ctactcaatc aaagaattct tctgggaaga agaaagtgaa ggtggattgg
     2761 acaccagagc tgcacagaag atttgtacaa gcagtggagc agctaggtgt ggataaggca
     2821 gtcccttcaa gaattcttga acttatggga attgattgtc ttactcgcca caacattgct
     2881 agccatcttc aaaaatacag atcccacagg aaacatttgc tagcaagaga agctgaggca
     2941 gcaaattgga gccagaggcg gcaattatac ggaggcactg cagcagcagc aggaggaggg
     3001 aagagagatg taaatccatg gcttgctcca accatgggat ttcctcctcc catggcaccg
     3061 gcggccgtcc ctcattttag acccttacat gtatggggtc acccatctgt cgaccaatct
     3121 ttaatgcacg tttggcctaa acatttagct cctccgcccc gaccaccaac atggccacca
     3181 actccaacac caacaccaac accaccacca ccaccgccag atcctaattc cttttggcat
     3241 tcacctcacc aacgtgtacc aaaccctctt accccaggaa cgccttgctt tccgccccct
     3301 ttaacaccag cgagatttcc aacaccgccg gtccccggaa taccaccacc tccaatgtac
     3361 aaagtagacc cggtcgcagg catgggtgtg ccggcacctg ctaccggaca agcagtaccc
     3421 catgcacctc ctcttgactt tcacccctcg acggagagcg tagatgcagc tatcggagat
     3481 gttttatcga aaccctggct gccacttcct cttgggctga aaccgccgtc gattgacagt
     3541 gtactggtgg agctacagcg gcaaggaata ccgaaaattc caccagtttg tacgtaagct
     3601 ttgagaccac gaa
//
