LOCUS       Exported                8346 bp ds-DNA     circular UNA 05-JUN-2023
DEFINITION  coverage: 258x.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 5, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8346
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     CDS             complement(789..1580)
                     /codon_start=1
                     /label=NPTII_KanR
                     /note="color: #993366"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     promoter        complement(1581..1685)
                     /label=AmpR Promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(1747..1941)
                     /direction=LEFT
                     /label=pVS1 Ori
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /note="color: #ffff00"
     CDS             complement(2007..3074)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /note="color: #993366"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(3509..4138)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /note="color: #993366"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4333..4460
                     /label=RB_Truncated
                     /note="/feature_type=TDNA Border"
                     /note="color: #a6acb3"
     terminator      complement(4506..4757)
                     /label=MAS terminator
                     /label=mas term
                     /note="/vntifkey=43"
                     /note="color: #ffffff; direction: LEFT"
     CDS             complement(4760..6130)
                     /codon_start=1
                     /label=CrGLK
                     /note="color: #993366"
                     /translation="MLAVSPLRSNNNERKKEICEISSSTSFPVVDDFPDFGGANLLDSI
                     DFDELFVGINGEDDVLPDLEMDPHDILAEFSLTESLSPSVSLDHQHHEHSQITSSISAA
                     DYDKLVELEDKANSSSASDDVGSGSSTLTQGEDVVSKSTVLNPSSKEGDKGRKSSSTQS
                     KNSSGKKKVKVDWTPELHRRFVQAVEQLGVDKAVPSRILELMGIDCLTRHNIASHLQKY
                     RSHRKHLLAREAEAANWSQRRQLYGGTAAAAGGGKRDVNPWLAPTMGFPPPMAPAAVPH
                     FRPLHVWGHPSVDQSLMHVWPKHLAPPPRPPTWPPTPTPTPTPPPPPPDPNSFWHSPHQ
                     RVPNPLTPGTPCFPPPLTPARFPTPPVPGIPPPPMYKVDPVAGMGVPAPATGQAVPHAP
                     PLDFHPSTESVDAAIGDVLSKPWLPLPLGLKPPSIDSVLVELQRQGIPKIPPVCTGS"
     CDS             complement(4760..4766)
                     /codon_start=1
                     /label=Stop Codon
                     /note="color: #993366"
                     /translation="FV"
     variation       5657
                     /label=Mutation A>T
                     /label=nonstandard type:  mutation
                     /note="For MoClo domestication"
                     /note="color: #a6acb3"
     5'UTR           complement(6140..6201)
                     /label=TMV Omega
                     /label=Omega leader
                     /note="/vntifkey=52"
                     /note="color: #a6acb3; direction: LEFT"
     promoter        complement(6218..6970)
                     /label=2x CaMV 35S promoter
                     /label=2x35S pro
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    7002..7152
                     /label=LB
                     /note="/feature_type=TDNA Border"
                     /note="color: #a6acb3"
     CDS             complement(join(8068..8346,1..525))
                     /codon_start=1
                     /label=VirGN54D
                     /note="color: #993366"
                     /translation="MIVHPSRENFSSAVNKGSDFRLKGEPLKHVLLVDDDVAMRHLIIE
                     YLTIHAFKVTAVADSTQFTRVLSSATVDVVVVDLDLGREDGLEIVRNLAAKSDIPIIII
                     SGDRLEETDKVVALELGASDFIAKPFSIREFLARIRVALRVRPNVVRSKDRRSFCFTDW
                     TLNLRQRRLMSEAGGEVKLTAGEFNLLLAFLEKPRDVLSREQLLIASRVRDEEVYDRSI
                     DVLILRLRRKLEADPSSPQLIKTARGAGYFFDADVQVSHGGTMAA"
ORIGIN
        1 ttcggacatc aagcgacgtt gcctgagatt aagtgtccag tcagtaaaac aaaaagaccg
       61 tcggtctttg gagcggacaa cgttggggcg cacgcgcaag gcaacccgaa tgcgtgctag
      121 aaactctctg atactgaacg gcttagcgat aaaatcactt gctcctagct cgagtgcaac
      181 aactttatcc gtctcctcaa ggcggtcgcc actgataatt atgattggaa tatcagactt
      241 tgccgccaga ttacgaacga tctcgagccc atcttcacga cctaaatcta gatcaacaac
      301 cacgacatcg accgtcgcgg aagagagtac tcttgtgaac tgggtgctgt cggctaccgc
      361 ggtcactttg aaggcgtgga tcgtaaggta ttcaataata agatgccgca tagcgacgtc
      421 gtcatcgaca agaagaacgt gtttcaacgg ctcacctttc aatctaaaat ctgaaccctt
      481 gttcacagcg cttgagaaat tttcacgtga aggatgtaca atcatctcca gctaaatggg
      541 cagttcgtca gaattgcggc tgaccgcgga tgacaaaaat gcgaaccaag tatttcaatt
      601 ttatgacaaa aattctcaat cgttgttaca agtgaaacgc ttcgaggtta cagctagaac
      661 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
      721 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
      781 gacagctaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
      841 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgagc
      901 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
      961 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
     1021 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
     1081 tctttaaatg gagtgtcctc ttcccagttt tcgcaatcca catcggccag atcgttattc
     1141 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
     1201 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
     1261 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
     1321 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
     1381 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
     1441 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
     1501 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
     1561 gatattctca ttttagccat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1621 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1681 ccgcgcactc ggagccttga tgtgggcgct ttttgcagct gttcggctgt gcgctggcca
     1741 gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag
     1801 gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca
     1861 atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc
     1921 ccaatgtacg tgctatccac aggaaagaag ctttttcgac ctttttcccc tgctagggca
     1981 atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg
     2041 ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac
     2101 tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct
     2161 ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct
     2221 gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa
     2281 tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa
     2341 tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag
     2401 cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc
     2461 gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct
     2521 ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg
     2581 aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga
     2641 tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct
     2701 gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg
     2761 tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc
     2821 acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta
     2881 atcgacgacg caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc
     2941 gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc
     3001 gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg
     3061 gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg
     3121 gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc
     3181 attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta
     3241 aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca
     3301 gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga
     3361 tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc
     3421 aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt
     3481 ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca
     3541 gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg
     3601 cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct
     3661 cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg
     3721 acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct
     3781 ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga
     3841 agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca
     3901 ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc
     3961 cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg
     4021 gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg
     4081 ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc
     4141 gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg
     4201 caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc
     4261 ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg
     4321 cagccgcacg gctggcacat acaaatggac gaacggataa accttttcac gcccttttaa
     4381 atatccgatt attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt
     4441 caaacactga tagtttaaac ggatcacttc gtgtccctcg gtcacatgtg catcctcttg
     4501 cagcggataa tttatttgaa aattcataag aaaagcaaac gttacatgaa ttgatgaaac
     4561 aatacaaaga cagataaagc cacgcacatt taggatattg gccgagatta ctgaatattg
     4621 agtaagatca cggaatttct gacaggagca tgtctacaat tcagcccaaa tggcagttga
     4681 aatactcaaa ccgccccata tgcaggagcg gatcattcat tgtttgtttg gttgcctttg
     4741 ccaacatggg agtccaagct tacgaacccg tacaaactgg tggaattttc ggtattcctt
     4801 gccgctgtag ctccaccagt acactgtcaa tcgacggcgg tttcagccca agaggaagtg
     4861 gcagccaggg tttcgataaa acatctccga tagctgcatc tacgctctcc gtcgaggggt
     4921 gaaagtcaag aggaggtgca tggggtactg cttgtccggt agcaggtgcc ggcacaccca
     4981 tgcctgcgac cgggtctact ttgtacattg gaggtggtgg tattccgggg accggcggtg
     5041 ttggaaatct cgctggtgtt aaaggtggcg gaaagcaagg cgttcctggg gtaagagggt
     5101 ttggtacacg ttggtgaggt gaatgccaaa aggaattagg atctggcggt ggtggtggtg
     5161 gtgttggtgt tggtgttgga gttggtggcc atgttggtgg tcggggcgga ggagctaaat
     5221 gtttaggcca aacgtgcatt aaagattggt cgacagatgg gtgaccccat acatgtaagg
     5281 gtctaaaatg agggacggcc gccggtgcca tgggaggagg aaatcccatg gttggagcaa
     5341 gccatggatt tacatctctc ttccctcctc ctgctgctgc tgcagtgcct ccgtataatt
     5401 gccgcctctg gctccaattt gctgcctcag cttctcttgc tagcaaatgt ttcctgtggg
     5461 atctgtattt ttgaagatgg ctagcaatgt tgtggcgagt aagacaatca attcccataa
     5521 gttcaagaat tcttgaaggg actgccttat ccacacctag ctgctccact gcttgtacaa
     5581 atcttctgtg cagctctggt gtccaatcca ccttcacttt cttcttccca gaagaattct
     5641 ttgattgagt agaagatgac ttccttcctt tatcaccttc tttagaagat ggatttaaaa
     5701 ccgttgattt gctaacaaca tcttctcctt gagttaaagt agatgagcct gaaccgacgt
     5761 cgtctgaagc ggatgatgaa ttagccttat cctctaattc tactagttta tcataatcag
     5821 ccgccgaaat tgaggaggta atttgagaat gttcatgatg ttgatgatca agagaaacag
     5881 atggtgataa tgattcagta agtgagaatt cagcaagaat atcatgagga tccatttcca
     5941 aatctggtaa aacatcatcc tcaccgttaa tcccaacaaa aagttcatca aaatcaatgc
     6001 tatcaagcaa atttgctccc ccaaaatcag gaaaatcatc aacaactgga aaactagtac
     6061 tactactaat ttcacatatc tccttttttc tctcattatt attgctcctc aatggtgaca
     6121 cagcaagcat tgtatcgata attgtaaatg taattgtaat gttgtttgtt gtttgttgtt
     6181 gttggtaatt gttgtaaaaa tgagctctta tactcgagcg tgtcctctcc aaatgaaatg
     6241 aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta
     6301 cgtcagtgga gatgtcacat caatccactt gctttgtaga cgtggttgga acctcttctt
     6361 tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagagagat
     6421 cttgaatgat agcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt
     6481 ctactgtcct ttcgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg
     6541 aaattatcct ttgttgaaaa gtctcaatag ccctttgatc ttctgagact gtatctttga
     6601 catttttgga gtagaccaga gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct
     6661 ttgtagacgt ggttggaacc tcttcttttt ccacgatgct cctcgtgggt gggggtccat
     6721 ctttgggacc actgtcggca gagagatctt gaatgatagc ctttccttta tcgcaatgat
     6781 ggcatttgta ggagccacct tccttttcta ctgtcctttc gatgaagtga cagatagctg
     6841 ggcaatggaa tccgaggagg tttcccgaaa ttatcctttg ttgaaaagtc tcaatagccc
     6901 tttgatcttc tgagactgta tctttgacat ttttggagta gaccagagtg tcgtgctcca
     6961 ccatgttgac ctccggatcc gaattcggca cagagtgttc aaccccagta cattaaaaac
     7021 gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg
     7081 ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca
     7141 ggcagcccat caaccatgac tactgtccgg tccaacagat ccgagcagga accgtaaaaa
     7201 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7261 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7321 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7381 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7441 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7501 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7561 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7621 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     7681 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7741 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7801 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgag atcacgagcc
     7861 agtatcctga cgaatgggtt ctcgtccaac gcccctttat cgacagtatg tgaatttgca
     7921 gtgtggccgc gatcaacgca cactggcctt gggtctgaag ggtgatcttc aaaacgaacc
     7981 cccgtcttgg tggtcagtgt gatgggcaca tcgcttactc ctcagtcacc ctttgagcca
     8041 gataattaag aggcaaatgc aattggctca ggctgccatc gtccccccgt gcgaaacctg
     8101 cacgtccgcg tcaaagaaat aaccggcacc tcttgctgtt tttatcagtt gagggcttga
     8161 cggatccgcc tcaagtttgc ggcgcagcct caaaatgaga acatctatac tcctgtcata
     8221 aacctcctcg tcgcgtactc gactggcaat gagaagttgc tcgcgcgata gaacgtcgcg
     8281 gggtttctct aaaaacgcga ggagaagatt gaactcacct gccgtaagtt tcacctcacc
     8341 gccagc
//
