LOCUS       Exported               11314 bp DNA     circular SYN 07-JUN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11314)
  AUTHORS   Jared Toettcher
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2023 from SnapGene 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1403..1810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     source          1403..1810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     source          1403..1810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     source          1403..1810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    550..729
                     /gene="cPPT"
                     /label=cPPT
     promoter        762..1255
                     /gene="pSFFV"
                     /label=SFFV
                     /note="PROMOTER "
     misc_feature    1334..2389
                     /label=Gal4LOV(SK22)-VP64
                     /label=Gal4VP64
     CDS             1334..2197
                     /codon_start=1
                     /label=Gal4D4S22
                     /translation="KLLSSIEQACDICRLKKLKCSASLERIEKNFVITDPRLPDNPIIF
                     ASDSFLQLTEYSREEILGRNCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGK
                     KFWNLFHLQPMRDQKGDVQYFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAASCS
                     KEKPKCAKCLKNNWECRYSPKTKRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMIL
                     KMDSLQDIKALLTGLFVQDNVNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKG
                     QRQLTVS"
     misc_feature    1334..1396
                     /label=Gal4(1-22)
     misc_feature    1397..1402
                     /label=Linker
     misc_feature    1403..1810
                     /label=AsLOV2(408-543)
     misc_feature    1811..1822
                     /label=Linker
     misc_feature    1823..2197
                     /label=Gal4(23-147)
     CDS             2237..2389
                     /codon_start=1
                     /label=VP64
                     /translation="ALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFD
                     LDMLGS"
     misc_feature    2409..2982
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2987..3691
                     /label=mCherry
     misc_feature    3755..4346
                     /gene="wPRE"
                     /label=WPRE
     gene            5577..6437
                     /gene="AmpR"
                     /label=bla (AmpR)
                     /note="CDS "
ORIGIN
        1 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
       61 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
      121 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
      181 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
      241 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
      301 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
      361 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
      421 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
      481 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
      541 ctcgacggtc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
      601 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
      661 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
      721 ccagtttgga tcgataagct tgatatcgaa ttcctgcagc cccgataaaa taaaagattt
      781 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggtta tggcaagcta
      841 gctgcagtaa cgccattatt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt
      901 cagatcaagg gcgggtacat gaaaatagct aacgtagggc caaacaggat atctgcggtg
      961 agcagtttcg gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc
     1021 ccgaggccaa gagcagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc
     1081 catcagatgt ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta
     1141 accaatcagc ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag
     1201 agctcacaac ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccggggggga
     1261 tctggagctc tcgagaattc tcacgcgtca agtggagcaa ggcaggtgga cagtggatcc
     1321 ggtcgccacc atgaagctgc tgagcagcat cgagcaggcc tgtgacatct gccggctgaa
     1381 gaaactgaag tgcagtgcat ctttggaacg tatcgaaaag aattttgtca tcacggatcc
     1441 gcgtcttccc gacaatccga ttatcttcgc gtcagactct ttcttacaac tgactgagta
     1501 tagtagagag gagatattgg ggcgtaactg tagatttctt caggggccag aaactgatcg
     1561 ggctaccgtt cgcaagatac gtgacgcaat agacaaccag accgaggtga cggtgcagct
     1621 gattaactac acaaagtctg ggaagaagtt ctggaacctg tttcatttac aacctatgag
     1681 agatcaaaaa ggtgacgttc aatatttcat cggggttcag ttagatggga ctgagcacgt
     1741 gagagatgca gcagaaagag agggtgtaat gcttattaaa aaaacagccg agaatatcga
     1801 cgaagccgct gcgtcatgca gcaaagaaaa gcccaagtgc gccaagtgcc tgaagaacaa
     1861 ctgggagtgc cggtacagcc ccaagaccaa gagaagcccc ctgaccagag cccacctgac
     1921 cgaggtggaa agccggctgg aaagactgga acagctgttt ctgctgatct tcccacgcga
     1981 ggacctggac atgatcctga agatggacag cctgcaggac atcaaggccc tgctgaccgg
     2041 cctgttcgtg caggacaacg tgaacaagga cgccgtgacc gacagactgg ccagcgtgga
     2101 aaccgacatg cccctgaccc tgcggcagca cagaatcagc gccaccagca gcagcgagga
     2161 aagcagcaac aagggccagc ggcagctgac agtgtctgct gctgcaggcg gaagcggagg
     2221 ctctggcgga tctgatgccc tggacgactt cgacctggat atgctgggca gcgacgccct
     2281 ggatgatttt gatctggaca tgctgggatc tgacgctctg gacgatttcg atctcgacat
     2341 gttgggatca gatgcactgg atgactttga cctggacatg ctcggatcat gagtaccaag
     2401 ctgatccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     2461 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     2521 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     2581 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     2641 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     2701 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     2761 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     2821 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     2881 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     2941 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggtga gcaagggcga
     3001 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     3061 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     3121 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     3181 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     3241 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     3301 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     3361 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     3421 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     3481 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     3541 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     3601 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     3661 ctccaccggc ggcatggacg agctgtacaa gtagctgagg ccgcgactct agagtcgacc
     3721 tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat tacaaaattt
     3781 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3841 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3901 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3961 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4021 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4081 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4141 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     4201 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4261 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4321 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc aaggcagctg tagatcttag
     4381 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     4441 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     4501 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     4561 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     4621 agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta
     4681 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat
     4741 atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg
     4801 taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc
     4861 ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac
     4921 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
     4981 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
     5041 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
     5101 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
     5161 atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctagctctg
     5221 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5281 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     5341 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     5401 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     5461 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5521 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5581 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5641 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5701 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5761 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5821 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5881 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5941 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6001 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6061 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6121 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6181 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6241 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6301 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6361 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6421 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6481 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6541 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6601 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6661 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6721 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     6781 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6841 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6901 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6961 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7021 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7081 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7141 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7201 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7261 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7321 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7381 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa
     7441 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7501 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     7561 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     7621 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7681 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7741 aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga
     7801 tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg
     7861 actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt
     7921 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa
     7981 aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga
     8041 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc
     8101 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga
     8161 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt
     8221 catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta
     8281 tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga
     8341 ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt
     8401 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt
     8461 tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca
     8521 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct
     8581 cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg
     8641 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca
     8701 ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta
     8761 ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt
     8821 gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
     8881 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa
     8941 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag
     9001 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt
     9061 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta
     9121 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
     9181 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac
     9241 aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat
     9301 aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct
     9361 caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg
     9421 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     9481 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     9541 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9601 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9661 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
     9721 caccccatac cctattacca ctgccaatta cctagtggtt tcatttactc taaacctgtg
     9781 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
     9841 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
     9901 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     9961 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
    10021 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
    10081 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
    10141 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
    10201 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
    10261 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
    10321 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    10381 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    10441 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
    10501 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
    10561 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
    10621 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
    10681 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
    10741 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
    10801 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
    10861 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
    10921 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
    10981 gcacagcaag cggccggtga tcttcagacc tggacgatat atatgaggga caattggaga
    11041 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
    11101 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
    11161 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
    11221 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
    11281 gcgcaacagc atctgttgca actcacagtc tggg
//
