LOCUS       426601502               3875 bp    DNA     circular SYN 12-Dec-2022
DEFINITION  TEF1pro-BleMX6-TEFterm-codon opt in pUCIDT-AMP
KEYWORDS    .
SOURCE      Integrated DNA Technologies
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="pUCIDT-AMP"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /note="M13 Forward (-41)"
                     /function="Primer"
     primer_bind     379..395
                     /note="M13 Forward (-20)"
                     /function="Primer"
     gene            483..1605
                     /note="TEF1pro-BleMX6-TEFterm-codon opt"
                     /function="Reference Gene"
     primer_bind     complement(1654..1670)
                     /note="M13 Reverse (-27)"
                     /function="Primer"
     primer_bind     complement(1667..1689)
                     /note="M13 Reverse (-48)"
                     /function="Primer"
     rep_origin      2041..2655
                     /note="pMB1 Origin"
     gene            complement(2815..3675)
                     /note="Ampicillin"
                     /function="Drug Resistance"
ORIGIN
         1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
        61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
       121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
       181 accaaatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
       241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcatcgctat
       301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
       361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcaac gcgatgacga tggatagcga
       421 ttcatcgatg agctgacccg atcgccgccg ccggagggtt gcgtttgaga cgggcgacag
       481 atggcgcgcc agatctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat
       541 ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga
       601 ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt
       661 tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg
       721 aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat
       781 aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct
       841 aggatacagt tctcacatca catccgaaca taaacaacca tggctaaact tacaagtgct
       901 gtacctgtat taaccgctag agatgttgca ggtgcagtag agttctggac tgatcgttta
       961 gggtttagta gagattttgt ggaggacgat tttgcaggtg ttgtacgtga tgatgtgacc
      1021 ttgtttatat ctgctgttca agatcaagtc gtaccagata atactttggc ctgggtctgg
      1081 gtacgaggtc ttgatgagtt gtacgccgaa tggtccgagg tagtcagtac caattttaga
      1141 gacgcttcag gtcctgctat gaccgagata ggagagcagc cttgggggag agagtttgct
      1201 cttcgtgatc cagcaggtaa ttgtgtacac ttcgtcgcag aagaacagga ctgatcagta
      1261 ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg
      1321 tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca
      1381 tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa
      1441 tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatcccgag ctcgaattca
      1501 tcgatgatat cagatccact agtggcctat gcggccgcgg atctgccggt ctccctatag
      1561 tgagtcgtat taatttcgat aagccaggtt aacctgcatt aatgaatcag ttctggacca
      1621 gcgagctgtg ctgcgactcg tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
      1681 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
      1741 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
      1801 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      1861 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      1921 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      1981 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      2041 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      2101 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      2161 aagctccctc gtgcgctctc ctgttccgac cctgtcgctt accggatacc tgtccgcctt
      2221 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      2281 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      2341 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      2401 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      2461 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      2521 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      2581 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      2641 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      2701 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
      2761 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
      2821 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
      2881 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
      2941 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
      3001 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
      3061 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
      3121 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
      3181 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
      3241 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
      3301 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
      3361 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
      3421 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
      3481 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
      3541 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
      3601 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
      3661 atgttgaata ctcatactct acctttttca atattattga agcatttatc agggttattg
      3721 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
      3781 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
      3841 ctataaaaat aggcgtatca cgaggccctt tcgtc
//
