LOCUS       pMRTK-clo5              1615 bp ds-DNA     circular     07-JUN-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(41..143)
                     /label="Cat Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          48..76
                     /label="JSpr017"
                     /note="sequence: TCCTTAGCTCCTGAAAATCTCGATAACTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          48..76
                     /label="JSpr131"
                     /note="sequence: TCCTTAGCTCCTGAAAATCTCGATAACTC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(119..143)
                     /label="JSpr060"
                     /note="sequence: TGATCGGCACGTAAGAGGTTCCAAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(119..143)
                     /label="JSpr391"
                     /note="sequence: TGATCGGCACGTAAGAGGTTCCAAC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(122..143)
                     /label="JSpr101"
                     /note="sequence: cttcacggtctcgATTCCGTTGATCGGCACGTAAGAGGTTCC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(122..147)
                     /label="JSpr406"
                     /note="sequence: cttcacGGTCTCgCTAGTGATCGGCACGTAAGAGGTTCC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(124..144)
                     /label="JSpr070"
                     /note="sequence: GTTGATCGGCACGTAAGAGGTT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(126..143)
                     /label="JSpr094"
                     /note="sequence: tgatcggcacgtaagagg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(126..143)
                     /label="JSpr101"
                     /note="sequence: GTAAGGGGTCTCCTtgatcggcacgtaagagg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    144..147
                     /label="GG Overhang"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(149..154)
                     /label="BsaI Binding Site"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    314..423
                     /label="oriT"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          528..550
                     /label="JSpr448"
                     /note="sequence: cttcacGGTCTCgCTCTggcgtagaggatctgaagatcag"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(536..560)
                     /label="JSpr498"
                     /note="sequence: ggttgaactgctgatcttcagatcc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    537..925
                     /label="R6K gamma oriR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication"
     primer          819..838
                     /label="JSpr446"
                     /note="sequence: taaggtttaacggttgtgga"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          854..878
                     /label="JSpr499"
                     /note="sequence: gtaacgcactgagaagcccttagag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    930..935
                     /label="BsaI Binding Site"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    937..940
                     /label="GG Overhang"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          937..959
                     /label="JSpr454"
                     /note="sequence: cttcacGGTCTCgAGAGtcacactggctcaccttcg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          937..961
                     /label="JSpr463"
                     /note="sequence: tagagtcacactggctcaccttcggg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          940..957
                     /label="JSpr098"
                     /note="sequence: AGTCGAGGTCTCGTGgtcacactggctcacctt"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          940..959
                     /label="JSpr017"
                     /note="sequence: gtcacactggctcaccttcg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          940..959
                     /label="JSpr090"
                     /note="sequence: TGACTCACACTGGCTCACCTTCG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(941..965)
                     /label="JSpr093"
                     /note="sequence: CCCACCCGAAGGTGAGCCAGTGTGA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          943..963
                     /label="JSpr007"
                     /note="sequence: TGACACTGGCTCACCTTCGGGTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    948..975
                     /label="T7Te Terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(963..989)
                     /label="JSpr012"
                     /note="sequence: CTCCTAGGTATAAACGCAGAAAGGCCC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     CDS             complement(999..40)
                     /label="CmR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1007..1028)
                     /label="JSpr074"
                     /note="sequence: CAGTACTGCGATGAGTGGCAGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1007..1028)
                     /label="JSpr428"
                     /note="sequence: CAGTACTGCGATGAGTGGCAGG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1014..1037
                     /label="JSpr028"
                     /note="sequence: ctcatcgcagtactgttgtaattc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1134..1153
                     /label="JSpr009"
                     /note="sequence: GCCCATGGTGAAAACGGGGG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1421..1440)
                     /label="JSpr073"
                     /note="sequence: aggtctcgATTCCGTATGGCAATGAAAG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          1435..1456
                     /label="JSpr074"
                     /note="sequence: tggtctcgGAATTCCGGATGAGCATTCA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1452..1473
                     /label="JSpr015"
                     /note="sequence: attcatcaggcgggcaagaatg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(1612..22)
                     /label="JSpr029"
                     /note="sequence: ggatataccaccgttgatatatccca"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 atatatcaac ggtggtatat ccagtgattt ttttctcCAT tttagcttcc ttagctcctg
       61 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
      121 tggaacctct tacgtgccga tcaAGCGcGA GACCgtcaat tcgaggggga tcaattccgt
      181 tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct
      241 ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc
      301 aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct
      361 tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc
      421 cggctaccgc cggcgtaaca gatgagggca agcggatggc tgatgaaacc aagccaacca
      481 ggaagggcag ccacctatca cggaattgat ccggccacga tgcgtccggc gtagaggatc
      541 tgaagatcag cagttcaacc tgttgatagt acgtactaag ctctcatgtt tcacgtacta
      601 agctctcatg tttaacgtac taagctctca tgtttaacga actaaaccct catggctaac
      661 gtactaagct ctcatggcta acgtactaag ctctcatgtt tcacgtacta agctctcatg
      721 tttgaacaat aaaattaata taaatcagca acttaaatag cctctaaggt tttaagtttt
      781 ataagaaaaa aaagaatata taaggctttt aaagctttta aggtttaacg gttgtggaca
      841 acaagccagg gatgtaacgc actgagaagc ccttagagcc tctcaaagca attttgagtg
      901 acacaggaac acttaacggc tgacatgggG GTCTCgCTCC tcacactggc tcaccttcgg
      961 gtgggccttt ctgcgtttat acctaggagt cAAAAttacg ccccgccctg ccactcatcg
     1021 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
     1081 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     1141 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     1201 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag
     1261 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     1321 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     1381 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat
     1441 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     1501 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     1561 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttggg
//