LOCUS       Exported                8789 bp DNA     circular SYN 07-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Yihao Yang
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2023 from SnapGene 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8789
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     rep_origin      924..1512
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             1695..1787
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    1800..1821
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1836..1866
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1874..1890
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     primer_bind     1898..1914
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1935..1953
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        1981..2207
                     /label=RSV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     LTR             2208..2388
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    2435..2560
                     /label=HIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             2544..2907
                     /codon_start=1
                     /label=HIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.3"
                     /note="/match_length=24.2"
                     /note="/other=CDS"
                     /translation="MGARASVLSGGELDRDGKKFG*GQGERKNIN*NI*YGQAGS*NDS
                     QLILAC*KHQKAVDKYWDSYNHPFRQDQKNLDHYIIQ*QPSIVCIKG*R*KTPRKL*TR
                     *RKSKTKVRPPHSKRPL"
     misc_feature    3053..3286
                     /label=RRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             3471..3515
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3663..3763
                     /codon_start=1
                     /label=Rev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
                     /translation="THLPTPRGPDRPEGIEEEGGERDRDRSIRLVNG"
     misc_feature    3813..3930
                     /label=cPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        4368..4743
                     /label=TRE3G promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.2"
                     /note="/other=promoter"
     protein_bind    4373..4391
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4409..4427
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4445..4463
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4481..4499
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4517..4535
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4553..4571
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4589..4607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4735..4775
                     /label=Fragment.FOR
     misc_feature    4756..5461
                     /label=TOPO Cloning Site
                     /note="TOPO Cloning Site"
     regulatory      4757..4766
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4763..4795
                     /label=Myc Tag
                     /note="Myc Tag (should check sequence verification)"
     CDS             4766..4795
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4784..5461
                     /codon_start=1
                     /label=SOX9_HMGonly_STOP
                     /translation="EEDLNLLDPFMKMTDEQEKGLSGAPSPTMSEDSAGSPCPSGSGSD
                     TENTRPQENTFPKGEPDLKKESEEDKFPVCIREAVSQVLKGYDWTLVPMPVRVNGSSKN
                     KPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGKLWRLLNESEKRPFVEEAER
                     LRVQHKKDHPDYKYQPRRRKSVKNGQAEAEEATEQTHISPNAIFKALQADSPHSSSGMS
                     EVHS"
     misc_feature    4982..5098
                     /label=DIM
     misc_feature    5099..5335
                     /label=HMG
     primer_bind     complement(5443..5485)
                     /label=Fragment.REV
     promoter        5470..5973
                     /label=hPGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=98.6"
                     /note="/other=promoter"
     promoter        5475..5975
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             5983..6585
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFPATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             5989..6585
                     /codon_start=1
                     /label=PuroR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=99.5"
                     /note="/other=CDS"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFPATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    6602..7190
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             complement(7073..7084)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             7200..7286
                     /codon_start=1
                     /label=nef (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=75.9"
                     /note="/match_length=29.9"
                     /note="/other=CDS"
                     /translation="FKTNASVPLRPMTYKAAVDLSHFLKEKGG"
     LTR             7288..7521
                     /label=3' LTR (Delta-U3)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     polyA_signal    7593..7714
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     rep_origin      7754..7889
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        complement(7910..7928)
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             7936..8091
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.7"
                     /note="/other=CDS"
                     /translation="SLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQ
                     LRSLNGE"
     primer_bind     complement(7938..7954)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      8096..8551
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      8096..8524
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        8577..8681
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             join(8682..8789,1..753)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
       61 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
      121 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
      181 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
      241 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
      301 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
      361 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
      421 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
      481 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
      541 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
      601 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
      661 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
      721 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
      781 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
      841 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
      901 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      961 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1021 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1081 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1141 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1201 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1261 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1321 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1381 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1441 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1501 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1561 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1621 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1681 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     1741 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     1801 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     1861 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     1921 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     1981 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     2041 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     2101 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     2161 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     2221 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2281 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2341 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2401 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     2461 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     2521 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     2581 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     2641 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     2701 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     2761 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     2821 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     2881 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     2941 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     3001 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     3061 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     3121 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     3181 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     3241 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     3301 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     3361 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     3421 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     3481 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     3541 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     3601 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     3661 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     3721 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     3781 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     3841 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     3901 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     3961 gtttggttag taccgggccc ggtgctttgc tctgagccag cccaccagtt tggaatgact
     4021 cctttttatg acttgaattt tcaagtataa agtctagtgc taaatttaat ttgaacaact
     4081 gtatagtttt tgctggttgg gggaaggaaa aaaaatggtg gcagtgtttt tttcagaatt
     4141 agaagtgaaa tgaaaacttg ttgtgtgtga ggatttctaa tgacatgtgg tggttgcata
     4201 ctgagtgaag ccggtgagca ttctgccatg tcaccccctc gtgctcagta atgtacttta
     4261 cagaaatcct aaactcaaaa gattgatata aaccatgctt cttgtgtata tccggtctct
     4321 tctctgggta gtctcactca gcctgcattt ctgccatcga tggtaccttt actccctatc
     4381 agtgatagag aacgtatgaa gagtttactc cctatcagtg atagagaacg tatgcagact
     4441 ttactcccta tcagtgatag agaacgtata aggagtttac tccctatcag tgatagagaa
     4501 cgtatgacca gtttactccc tatcagtgat agagaacgta tctacagttt actccctatc
     4561 agtgatagag aacgtatatc cagtttactc cctatcagtg atagagaacg tataagcttt
     4621 aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt agtgaaccgt cagatcgcct
     4681 ggagcaattc cacaacactt ttgtcttata ccaactttcc gtaccacttc ctaccctcgt
     4741 aaagctagca ccggtcgcca ccatggaaca aaaacttatt tctgaagaag atctgaatct
     4801 cctggacccc ttcatgaaga tgaccgacga gcaggagaag ggcctgtctg gcgcccccag
     4861 ccccaccatg tcggaggact cggctggttc gccctgtccc tcgggctccg gctcggacac
     4921 ggagaacacc cggccccagg agaacacctt ccccaagggc gagccggatc tgaagaagga
     4981 gagcgaggaa gataagttcc ccgtgtgcat ccgcgaggcg gtcagccagg tgctgaaggg
     5041 ctacgactgg acgctggtgc ccatgcccgt gcgcgtcaac ggctccagca agaacaagcc
     5101 acacgtcaag cgacccatga acgccttcat ggtgtgggcg caggctgcgc gcaggaagct
     5161 ggcagaccag tacccgcatc tgcacaacgc ggagctcagc aagactctgg gcaagctctg
     5221 gaggctgctg aacgagagcg agaagagacc cttcgtggag gaggcggagc ggctgcgcgt
     5281 gcagcacaag aaagaccacc ccgattacaa gtaccagccc cggcggagga agtcggtgaa
     5341 gaacggacaa gcggaggccg aagaggccac ggaacagact cacatctctc ctaatgctat
     5401 cttcaaggcg ctgcaagccg actccccaca ttcctcctcc ggcatgagtg aggtgcactc
     5461 ctgaacgcgt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
     5521 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
     5581 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
     5641 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
     5701 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg
     5761 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
     5821 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
     5881 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
     5941 ccctcgttga ccgaatcacc gacctctctc cccgggggat ccatggccac cgagtacaag
     6001 cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc
     6061 gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg
     6121 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6181 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6241 gcggtgttcg ccgagatcgg ctcgcgcatg gccgagttga gcggttcccg gctggccgcg
     6301 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcccg
     6361 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6421 cccggagtgg aggcggccga gcgcgctggg gtgcccgcct tcctggagac ctccgcgccc
     6481 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6541 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaggatc ctctagatcc
     6601 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6661 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6721 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6781 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6841 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6901 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     6961 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     7021 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7081 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7141 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct
     7201 ttaagaccaa tgcatcggta cctttaagac caatgactta caaggcagct gtagatctta
     7261 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     7321 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7381 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7441 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7501 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     7561 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     7621 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7681 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     7741 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7801 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7861 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     7921 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     7981 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     8041 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     8101 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     8161 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     8221 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     8281 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     8341 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     8401 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     8461 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     8521 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     8581 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8641 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     8701 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     8761 cgctggtgaa agtaaaagat gctgaagat
//
