LOCUS       pEntr3c_Fray        3943 bp    DNA     circular     22-AUG-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-11906978|
COMMENT     VNTDBDATE|-11906978|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEntr3c_Fray|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     CDS             454..456
                     /vntifkey="4"
                     /label=frame\of\gateway\vectors
     primer_bind     301..320
                     /vntifkey="28"
                     /label=ENTR3C_for
     primer_bind     complement(2295..2318)
                     /vntifkey="28"
                     /label=ENTR_seq_rev
                     /note="suggested reverse sequencing primer by Invitrogen"
     misc_recomb     358..457
                     /invitrogen="2670000"
                     /vntifkey="86"
                     /label=attL1
     misc_recomb     complement(2172..2271)
                     /invitrogen="2680000"
                     /vntifkey="86"
                     /label=attL2
     CDS             2394..3203
                     /invitrogen="1210000"
                     /vntifkey="4"
                     /label=Kan(R)
     rep_origin      3267..3940
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     terminator      complement(106..149)
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     terminator      complement(281..308)
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     CDS             493..2151
                     /gene="fray"
                     /locus_tag="Dmel_CG7693"
                     /gene_synonym="4624; CG7693; Dmel\CG7693; dSTRAD;
                     l(3)07551; lag; STE20-like/SPAK"
                     /EC_number="2.7.11.-"
                     /codon_start=1
                     /product="frayed, isoform C"
                     /protein_id="NP_001138077.1"
                     /db_xref="GI:221379896"
                     /db_xref="FLYBASE:FBgn0023083"
                     /db_xref="GeneID:44233"
                     /vntifkey="4"
                     /label=fray
                     /note="CG7693 gene product from transcript CG7693-RC; CG7693-PC; fray-PC; laggard; Ste20-like protein kinase"
     primer_bind     532..550
                     /vntifkey="28"
                     /label=fray_RNAi_1
     primer_bind     complement(672..690)
                     /vntifkey="28"
                     /label=fray_RNAi_2
     mutation        1045..1047
                     /vntifkey="62"
                     /label=D185A\cat\inactive
     primer_bind     complement(2131..2152)
                     /vntifkey="28"
                     /label=fray_rev_sal
     misc_feature    493..510
                     /vntifkey="21"
                     /label=Fray_for_bam
BASE COUNT     1016 a       943 c      1016 g       968 t 
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctctt taaaggaacc aattcagtcg 
      481 actggatcca ccatgacctc catacccgcc aatctgtcta gcaataatgt tgctggtgca 
      541 gcaaccttac caggagctgc ggcaccgcca gagaaatata catggccgaa ctccaaggac 
      601 gattacgagc tgagggacgt cattggtgtg ggtgccacag ccgttgtcca tggggcttac 
      661 tgtattccaa ggaacgagaa gtgcgccatt aagcgcatca acctggagaa gtggaacaca 
      721 tcgatggacg agctgctcaa ggagattcag gccatgtcct cctgttttca cgagaacgtg 
      781 gtgacctatc acacctcatt tgtggttagg gaagagctct ggttggtgct aagacttcta 
      841 gaaggcggtt ctttgctgga catcatcaag cacaagatgc gaacgtccaa ctgcaaacag 
      901 ggtgtcttcg acgaagccac cattgccacg gtactgaagg aagtcctaaa gggattggaa 
      961 tatttccatt cgaacgggca aatccatcgt gatatcaagg ccggcaatat tttgattggt 
     1021 gacgatggca ccattcaaat cgctgacttc ggtgtgagtg catggctggc cactggcagg 
     1081 gatttgtcca ggcagaaagt gcgccacacc tttgtgggca caccgtgttg gatggctccc 
     1141 gaggtgatgg agcaggatca tggctatgac tttaaggcgg acatttggtc ctttggaata 
     1201 acagctatcg aaatggccac gggcactgca ccataccata aatacccgcc catgaaggtg 
     1261 ctaatgctga ccctgcaaaa tgatccgccc accttggaca cgggtgcgga cgataaagat 
     1321 caatataagg cgtatggcaa gacattccgc aagatgatcg tcgagtgctt gcaaaaggag 
     1381 ccatcgaaac gtcccacggc tagtgagctt ttgaagcacg ctttcttcaa gaaggccaag 
     1441 gatcgcaaat atctcaccca gacgttgctg caatctgggc ccagtatgga gaccagggtt 
     1501 cacaaggcgg cgaaacgcca accaggagcc tcgggccgtt tgcatcgcac ggtgacaggc 
     1561 gaatgggtct ggtctagcga agaggaggac aatggtggct ctgccaccgg ttccggaaca 
     1621 ggagacagaa aacatccatc ttcggactcg gattccgagg atagacccat gaatcgattg 
     1681 gaaagagccg actcatcgga cagcgaccgg gaagagcctt ctcccgaaat aacccacagt 
     1741 gtttcctcag ccacagtgac gcctggagct ccagctgctg ctggtgctgg tgcagcccaa 
     1801 gagctaacag ctggcattgc tcaattacca ctacctagcg aagcggccgg cgaggcgcca 
     1861 ccagtcaatc ttgtccttcg catgcgcaac ttgcgacgtg aacttcacga cattcgcttc 
     1921 gagttcgttg tgggcaagga cactgccgag ggtatcgcta cagaactggt agacgccgga 
     1981 ctcgttgatg cgctggatac ccaaccgatg gcaacgcatc tggatcaact gatagctgcc 
     2041 agtgcaacga tgaaaacgat tactttccaa ttgagctccg gcgtgcagcc gggcgaagtg 
     2101 cccgacgaac gatcactggt gggatatgcg cagatctcca tcacggacta gtgtcgactc 
     2161 gagatatcta gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc 
     2221 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc 
     2281 tgcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat 
     2341 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc 
     2401 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg 
     2461 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg 
     2521 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg 
     2581 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca 
     2641 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa 
     2701 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg 
     2761 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc 
     2821 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg 
     2881 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac 
     2941 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta 
     3001 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc 
     3061 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga 
     3121 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt 
     3181 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacat tattcagatt 
     3241 gggccccgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 
     3301 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 
     3361 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 
     3421 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 
     3481 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 
     3541 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 
     3601 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 
     3661 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 
     3721 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 
     3781 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 
     3841 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 
     3901 ttttacggtt cctggccttt tgctggcctt ttgctcacat gtt 
//
