LOCUS       pAGW_Fray        7946 bp    DNA     circular     22-AUG-2017
DEFINITION  attB1-attB2.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-11906981|
COMMENT     VNTDBDATE|-11906981|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAGW_Fray|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     misc_feature    6198..6218
                     /vntifkey="21"
                     /label=attB1
     misc_feature    complement(7939..13)
                     /vntifkey="21"
                     /label=attB2
     primer_bind     5384..5403
                     /vntifkey="28"
                     /label=Ac_for
     CDS             977..1834
                     /vntifkey="4"
                     /label=bla\(AmpR0
     polyA_site      38..880
                     /vntifkey="26"
                     /label=SV40\PA\site
     mat_peptide     14..26
                     /vntifkey="84"
                     /label=triple\stop
     CDS             5471..6187
                     /vntifkey="4"
                     /label=EGFP
     promoter        2868..5440
                     /vntifkey="29"
                     /label=Actin\5c\promoter
     misc_feature    6257..6274
                     /vntifkey="21"
                     /label=Fray_for_bam
     primer_bind     complement(7895..7916)
                     /vntifkey="28"
                     /label=fray_rev_sal
     mutation        6809..6811
                     /vntifkey="62"
                     /label=D185A\cat\inactive
     primer_bind     complement(6436..6454)
                     /vntifkey="28"
                     /label=fray_RNAi_2
     primer_bind     6296..6314
                     /vntifkey="28"
                     /label=fray_RNAi_1
     CDS             6257..7915
                     /gene="fray"
                     /locus_tag="Dmel_CG7693"
                     /gene_synonym="4624; CG7693; Dmel\CG7693; dSTRAD;
                     l(3)07551; lag; STE20-like/SPAK"
                     /EC_number="2.7.11.-"
                     /codon_start=1
                     /product="frayed, isoform C"
                     /protein_id="NP_001138077.1"
                     /db_xref="GI:221379896"
                     /db_xref="FLYBASE:FBgn0023083"
                     /db_xref="GeneID:44233"
                     /vntifkey="4"
                     /label=fray
                     /note="CG7693 gene product from transcript CG7693-RC; CG7693-PC; fray-PC; laggard; Ste20-like protein kinase"
     CDS             6218..6220
                     /vntifkey="4"
                     /label=frame\of\gateway\vectors
     CDS             5471..7912
                     /vntifkey="4"
                     /label=EGFP-Fray
BASE COUNT     2196 a      1874 c      1909 g      1967 t 
ORIGIN
        1 ttgtacaaag tggtgacgta agctagcagg atctttgtga aggaacctta cttctgtggt 
       61 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt 
      121 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc 
      181 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc 
      241 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct 
      301 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt 
      361 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag 
      421 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt 
      481 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg 
      541 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg 
      601 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac 
      661 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca 
      721 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata 
      781 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 
      841 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc ccgtttaaac tacgcgtaat 
      901 tcaaacaggg ttctggcgtc gttctcgtac tgttttcccc aggccagtgc tttagcgtta 
      961 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 
     1021 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 
     1081 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 
     1141 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 
     1201 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 
     1261 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 
     1321 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 
     1381 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 
     1441 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 
     1501 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 
     1561 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 
     1621 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 
     1681 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 
     1741 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 
     1801 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 
     1861 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 
     1921 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 
     1981 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 
     2041 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 
     2101 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 
     2161 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 
     2221 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 
     2281 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 
     2341 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 
     2401 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 
     2461 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 
     2521 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 
     2581 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 
     2641 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 
     2701 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 
     2761 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 
     2821 tcattaatgc aggcaactcg tgaaaggtag gcggatcagc ggccgcagca tgcaattcta 
     2881 tattctaaaa acacaaatga tacttctaaa aaaaaatcat gaatggcatc aactctgaat 
     2941 caaatctttg cagatgcacc tacttctcat ttccactgtc acatcatttt tccagatctc 
     3001 gctgcctgtt atgtggccca caaaccaaga cacgttttat ggccattaaa gctggctgat 
     3061 cgtcgccaaa caccaaatac ataatgaata tgtacacatt cgagaaagaa gcgatcaaag 
     3121 aagcgtcttc gggcggagta ggagaatgcg gaggagaagg agaacgagct gatctagtat 
     3181 ctctccacaa tccaatgcca actgaccaac tggccatatt cggagcaatt tgaagccaat 
     3241 ttccatcgcc tggcgatcgc tccattcttg gctatatgtt tttcaccgtt acccggggcc 
     3301 attttcaaag actcgtcggc aagataagat tgtgtcactc gctgtctctc ttcatttgtc 
     3361 gaagaatgct gaggaatttc gcgatgacgt cggcgagtat tttgaagaat gagaataatt 
     3421 tgtatttata cgaaaatcag ttagtggaat tttctacaaa aacatgttat ctatagataa 
     3481 ttttgttgca aaatatgttg actatgacaa agattgtatg tatatacctt taatgtattc 
     3541 tcattttctt atgtatttat aatggcaatg atgatactga tgatatttta agatgatgcc 
     3601 agaccaaaag gcttgaattt ctgcgtcttt tgccgaacgc agtgcatgtg caattgttgt 
     3661 tttttggaat attcaatttt cggactgtcc gctttgattt cagtttcttg gcttattcaa 
     3721 aaagcaaagt aaagccaaaa aagcgagatg gcaataccaa atgcggcaaa acggtagtgg 
     3781 aaggaaaggg gtgcggggca gcggaaggaa gggtggggcg gggcgtggcg gggtctgtgg 
     3841 ctgggcgcga cgtcaccgac gttggagcca ctcctttgac catgtgtgcg tgtgtgtatt 
     3901 attcgtgtct cgccactcgc cggttgtttt tttcttttta tgctgcgctc tctctagcgc 
     3961 catctcgctt acgcatgctc aacgcaccgc atgttgccgt ttccttttat gcgtcatttt 
     4021 ggctcgaaat aggcaattat ttaaacaaag attagtcaac gaaaacgcta aaataaataa 
     4081 gtctacaata tggttactta ttgccatgtg tgtgcagcca acgatagcaa caaaagcaac 
     4141 aacacaggtg gctttccctc tttcactttt tgtttgcaag ccgcgtgcga gcaagacggc 
     4201 acgaccggca aacgcaatta cgctgacaaa gagcagacga agttttggcg aaaaacatca 
     4261 aggcgcctga tacgaatgca tttgcaataa caattgcgat atttaatatt gtttatgaag 
     4321 ctgtttgact tcaaaacaca caaaaaaaaa aataaaacaa attatttgaa agagaattag 
     4381 gaatcggacg cttatcgtta gggtaacaac aagaaatgct tactgagtca cagcctctgg 
     4441 aaaactgccg caagccagag agagagagaa aaagagggag agcagcttag accgcatgtg 
     4501 cttgtgtgtg aggcgtctct ctcttcgtct ctgttgcgca aacgcataga ctgcactgag 
     4561 aaaatcgatt acctattttt tatgaatgaa tatttgcact attactattc aaaactatta 
     4621 agatagcaat cacattcaat agccaaatac tataccacct gagcgatgca acgaaatgat 
     4681 caatttgagc aaaaatgctg catatttagg acggcatcat tatagaaatg cttcttgctg 
     4741 tgtacttttc tctcgtctgg cagctgtttc gccgttattg ttaaaaccgg cttaagttag 
     4801 gtgtgttttc tacgactagt gaatgcccta ctagaagatg tgtgttgcac aaaatgtccc 
     4861 tggaataacc aatttgaagt gcagatagca gtaaacgtaa gctaatatga atattattta 
     4921 actgtaatgt tttaatatcg ctggacatta ctaataaacc cactataaac acatgtacat 
     4981 atgtatgttt tggcatacaa tgagtagttg gggaaaaaat gtgtaaaagc accgtgacca 
     5041 tcacagcata aagataacca gctgaagtat cgaatatgag taacccccaa attgaatcac 
     5101 atgccgcaac tgataggacc catggaagta cactcttcat ggcgatatac aagacacaca 
     5161 caagcacgaa cacccagttg cggaggaaat tctccgtaaa tgaaaaccca atcggcgaac 
     5221 aattcatacc catatatggt aaaagttttg aacgcgactt gagagcggag agcattgcgg 
     5281 ctgataaggt tttagcgcta agcgggcttt ataaaacggg ctgcgggacc agttttcata 
     5341 tcactaccgt ttgagttctt gtgctgtgtg gatactcctc ccgacacaaa gccgctccat 
     5401 cagccagcag tcgtctaatc cagagacccc ggatccagat atcgaggcct gtctagagaa 
     5461 gcccgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 
     5521 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 
     5581 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 
     5641 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga 
     5701 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 
     5761 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 
     5821 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 
     5881 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa 
     5941 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt 
     6001 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 
     6061 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga 
     6121 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 
     6181 gtacaagcac cggtatacaa gtttgtacaa aaaagcaggc tctttaaagg aaccaattca 
     6241 gtcgactgga tccaccatga cctccatacc cgccaatctg tctagcaata atgttgctgg 
     6301 tgcagcaacc ttaccaggag ctgcggcacc gccagagaaa tatacatggc cgaactccaa 
     6361 ggacgattac gagctgaggg acgtcattgg tgtgggtgcc acagccgttg tccatggggc 
     6421 ttactgtatt ccaaggaacg agaagtgcgc cattaagcgc atcaacctgg agaagtggaa 
     6481 cacatcgatg gacgagctgc tcaaggagat tcaggccatg tcctcctgtt ttcacgagaa 
     6541 cgtggtgacc tatcacacct catttgtggt tagggaagag ctctggttgg tgctaagact 
     6601 tctagaaggc ggttctttgc tggacatcat caagcacaag atgcgaacgt ccaactgcaa 
     6661 acagggtgtc ttcgacgaag ccaccattgc cacggtactg aaggaagtcc taaagggatt 
     6721 ggaatatttc cattcgaacg ggcaaatcca tcgtgatatc aaggccggca atattttgat 
     6781 tggtgacgat ggcaccattc aaatcgctga cttcggtgtg agtgcatggc tggccactgg 
     6841 cagggatttg tccaggcaga aagtgcgcca cacctttgtg ggcacaccgt gttggatggc 
     6901 tcccgaggtg atggagcagg atcatggcta tgactttaag gcggacattt ggtcctttgg 
     6961 aataacagct atcgaaatgg ccacgggcac tgcaccatac cataaatacc cgcccatgaa 
     7021 ggtgctaatg ctgaccctgc aaaatgatcc gcccaccttg gacacgggtg cggacgataa 
     7081 agatcaatat aaggcgtatg gcaagacatt ccgcaagatg atcgtcgagt gcttgcaaaa 
     7141 ggagccatcg aaacgtccca cggctagtga gcttttgaag cacgctttct tcaagaaggc 
     7201 caaggatcgc aaatatctca cccagacgtt gctgcaatct gggcccagta tggagaccag 
     7261 ggttcacaag gcggcgaaac gccaaccagg agcctcgggc cgtttgcatc gcacggtgac 
     7321 aggcgaatgg gtctggtcta gcgaagagga ggacaatggt ggctctgcca ccggttccgg 
     7381 aacaggagac agaaaacatc catcttcgga ctcggattcc gaggatagac ccatgaatcg 
     7441 attggaaaga gccgactcat cggacagcga ccgggaagag ccttctcccg aaataaccca 
     7501 cagtgtttcc tcagccacag tgacgcctgg agctccagct gctgctggtg ctggtgcagc 
     7561 ccaagagcta acagctggca ttgctcaatt accactacct agcgaagcgg ccggcgaggc 
     7621 gccaccagtc aatcttgtcc ttcgcatgcg caacttgcga cgtgaacttc acgacattcg 
     7681 cttcgagttc gttgtgggca aggacactgc cgagggtatc gctacagaac tggtagacgc 
     7741 cggactcgtt gatgcgctgg atacccaacc gatggcaacg catctggatc aactgatagc 
     7801 tgccagtgca acgatgaaaa cgattacttt ccaattgagc tccggcgtgc agccgggcga 
     7861 agtgcccgac gaacgatcac tggtgggata tgcgcagatc tccatcacgg actagtgtcg 
     7921 actcgagata tctagaccca gctttc 
//
