LOCUS       pMRTK-clo15             1806 bp ds-DNA     circular     07-JUN-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(115..217)
                     /label="Cat Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          122..150
                     /label="JSpr131"
                     /note="sequence: TCCTTAGCTCCTGAAAATCTCGATAACTC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(193..217)
                     /label="JSpr391"
                     /note="sequence: TGATCGGCACGTAAGAGGTTCCAAC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(275..299)
                     /label="JSpr498"
                     /note="sequence: ggttgaactgctgatcttcagatcc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    276..663
                     /label="R6K gamma oriR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication"
     primer          557..576
                     /label="JSpr446"
                     /note="sequence: taaggtttaacggttgtgga"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          592..616
                     /label="JSpr499"
                     /note="sequence: gtaacgcactgagaagcccttagag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(648..667)
                     /label="JSpr487"
                     /note="sequence: ggaattcccatgtcagccgttaagtg"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    668..673
                     /label="BsaI Binding Site"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     Promoter Swap   675..678
                     /label="GG Overhang"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    679..1194
                     /label="Elu ES2"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1195..1198
                     /label="GG Overhang"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(1200..1205)
                     /label="BsaI Binding Site"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(1206..1230)
                     /label="JSpr467"
                     /note="sequence: cccacccgaaggtgagccagtgtga"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1213..1240
                     /label="T7Te Terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(1264..114)
                     /label="CmR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1272..1293)
                     /label="JSpr428"
                     /note="sequence: CAGTACTGCGATGAGTGGCAGG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 CTGAACGGTC TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT
       61 ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATTTTAGC
      121 TTCCTTAGCT CCTGAAAATC TCGATAACTC AAAAAATACG CCCGGTAGTG ATCTTATTTC
      181 ATTATGGTGA AAGTTGGAAC CTCTTACGTG CCGATCAgtc aattcgaggg ggatcaattc
      241 cgtattgatc cggccacgat gcgtccggcg tagaggatct gaagatcagc agttcaacct
      301 gttgatagta cgtactaagc tctcatgttt cacgtactaa gctctcatgt ttaacgtact
      361 aagctctcat gtttaacgaa ctaaaccctc atggctaacg tactaagctc tcatggctaa
      421 cgtactaagc tctcatgttt cacgtactaa gctctcatgt ttgaacaata aaattaatat
      481 aaatcagcaa cttaaatagc ctctaaggtt ttaagtttta taagaaaaaa agaatatata
      541 aggcttttaa agcttttaag gtttaacggt tgtggacaac aagccaggga tgtaacgcac
      601 tgagaagccc ttagagcctc tcaaagcaat tttgagtgac acaggaacac ttaacggctg
      661 acatgggGGT CTCgGAGAgc ctgtcactga acggcaccat catcgtggcg cgcgacattg
      721 cccacgcgaa gctgaaagag ctgctcgaca acggtgaaga actgccgcag tatgtaaggg
      781 tttttcttag cgggttgctg gacgcactgg aatgattgag ctatgttaaa aaagtcttag
      841 ataacagcgg ttgtgaaagc aacggcgagt ctgtgtaagt gagcagggct gtccatatgt
      901 catgcgcata attgtgcaaa agcataccgg cacagttcga atttgtgcgg cggatcgaga
      961 catgtttaaa aatggcttgc catattaaac atagtgtgtg ataacacgtt ttgggtcaaa
     1021 cgaggtacag ttctgtttat gtgtggcatt ttcagtaaag aagtcctgag taaacacgtt
     1081 gtcgttgaat accgcttctc tgccgaacct tatattagtg cctcatgcag taatgtctca
     1141 gttttatctc agtaatgcct gcgggctaag aaaacactct aaggaatttt gcaaATTAcG
     1201 AGACCtcaca ctggctcacc ttcgggtggg cctttctgcg tttataccta ggagtcAAAA
     1261 TTACTCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA TTCTACCGAC
     1321 ATGGAAGCCA TCACAAACGG CATGATGAAC CTGAATCGCC AGCGGCATCA GCACCTTGTC
     1381 GCCTTGCGTA TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT CCATATTGGC
     1441 CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA AAAACATATT
     1501 CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA CATCTTGCGA
     1561 ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG ATGAAAACGT
     1621 TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA TCACCAGCTC
     1681 ACCGTCTTTC ATTGCCATAC GGAATTCCGG ATGAGCATTC ATCAGGCGGG CAAGAATGTG
     1741 AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA AGGCCGTAAT
     1801 ATCCAG
//