LOCUS       pMRTK-clo28             1644 bp ds-DNA     circular     07-JUN-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          213..232
                     /label="JSpr446"
                     /note="sequence: taaggtttaacggttgtgga"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          248..272
                     /label="JSpr499"
                     /note="sequence: gtaacgcactgagaagcccttagag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    324..329
                     /label="BsaI Binding Site"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     Promoter Swap   331..334
                     /label="GG Overhang"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    337..691
                     /label="BPI_ES3"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    688..691
                     /label="GG Overhang"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(693..698)
                     /label="BsaI Binding Site"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          695..717
                     /label="JSpr454"
                     /note="sequence: cttcacGGTCTCgAGAGtcacactggctcaccttcg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          695..719
                     /label="JSpr463"
                     /note="sequence: tagagtcacactggctcaccttcggg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          698..715
                     /label="JSpr098"
                     /note="sequence: AGTCGAGGTCTCGTGgtcacactggctcacctt"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          698..717
                     /label="JSpr017"
                     /note="sequence: gtcacactggctcaccttcg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    706..733
                     /label="T7Te Terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(757..1413)
                     /label="CmR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(765..786)
                     /label="JSpr428"
                     /note="sequence: CAGTACTGCGATGAGTGGCAGG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          772..795
                     /label="JSpr028"
                     /note="sequence: ctcatcgcagtactgttgtaattc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          892..911
                     /label="JSpr009"
                     /note="sequence: GCCCATGGTGAAAACGGGGG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1179..1198)
                     /label="JSpr073"
                     /note="sequence: aggtctcgATTCCGTATGGCAATGAAAG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          1193..1214
                     /label="JSpr074"
                     /note="sequence: tggtctcgGAATTCCGGATGAGCATTCA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1210..1231
                     /label="JSpr015"
                     /note="sequence: attcatcaggcgggcaagaatg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(1370..1395)
                     /label="JSpr029"
                     /note="sequence: ggatataccaccgttgatatatccca"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(1414..1516)
                     /label="Cat Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          1421..1449
                     /label="JSpr131"
                     /note="sequence: TCCTTAGCTCCTGAAAATCTCGATAACTC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1492..1516)
                     /label="JSpr391"
                     /note="sequence: TGATCGGCACGTAAGAGGTTCCAAC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(1495..1516)
                     /label="JSpr101"
                     /note="sequence: cttcacggtctcgATTCCGTTGATCGGCACGTAAGAGGTTCC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1495..1520)
                     /label="JSpr406"
                     /note="sequence: cttcacGGTCTCgCTAGTGATCGGCACGTAAGAGGTTCC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1499..1516)
                     /label="JSpr094"
                     /note="sequence: tgatcggcacgtaagagg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1499..1516)
                     /label="JSpr101"
                     /note="sequence: GTAAGGGGTCTCCTtgatcggcacgtaagagg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1566..1588
                     /label="JSpr448"
                     /note="sequence: cttcacGGTCTCgCTCTggcgtagaggatctgaagatcag"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(1574..1598)
                     /label="JSpr498"
                     /note="sequence: ggttgaactgctgatcttcagatcc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1575..319
                     /label="R6K gamma oriR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication"
ORIGIN
        1 catgtttaac gtactaagct ctcatgttta acgaactaaa ccctcatggc taacgtacta
       61 agctctcatg gctaacgtac taagctctca tgtttcacgt actaagctct catgtttgaa
      121 caataaaatt aatataaatc agcaacttaa atagcctcta aggttttaag ttttataagA
      181 aaaaaaagaa tatataaggc ttttaaagct tttaaggttt aacggttgtg gacaacaagc
      241 cagggatgta acgcactgag aagcccttag agcctctcaa agcaattttg agtgacacag
      301 gaacacttaa cggctgacat gggGGTCTCg GAGAcgcttt gcagactgct gctgacacca
      361 gtgtctcctc cattgcgacc atcaacactg gataagtcgc aaattcctct ttcttggcgc
      421 aggctgcctg aagctttctg gcctgctgca aattcctgtt gtcagatcct cccgataatg
      481 cgaactataa tgttacaatc cgatcattat gtttggatcg tttcatattt ctgtttgacc
      541 tgtcaagtat gcaccatcgc aattgcacca attgatgtgg caagctaaac gggggtgaac
      601 ggcaaccgaa atggggaatc agtgagcagg atcagtcgta aagacgaacg gattgccgaa
      661 cttgcggctc ttgttgagga agcgggaATT AcGAGACCtc acactggctc accttcgggt
      721 gggcctttct gcgtttatac ctaggagtcA AAATTACTCC CCGCCCTGCC ACTCATCGCA
      781 GTACTGTTGT AATTCATTAA GCATTCTACC GACATGGAAG CCATCACAAA CGGCATGATG
      841 AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT TGCCCATGGT
      901 GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC TGGTGAAACT
      961 CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA AACCCTTTAG GGAAATAGGC
     1021 CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT GCCGGAAATC
     1081 GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA AAACGGTGTA
     1141 ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA TACGGAATTC
     1201 CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT
     1261 ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT
     1321 ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC
     1381 AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT
     1441 CGATAACTCA AAAAATACGC CCGGTAGTGA TCTTATTTCA TTATGGTGAA AGTTGGAACC
     1501 TCTTACGTGC CGATCAgtca attcgagggg gatcaattcc gtattgatcc ggccacgatg
     1561 cgtccggcgt agaggatctg aagatcagca gttcaacctg ttgatagtac gtactaagct
     1621 ctcatgtttc acgtactaag ctct
//