LOCUS       pMRTK-clo32             1613 bp ds-DNA     circular     07-JUN-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    157..160
                     /label="GG Overhang"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(162..167)
                     /label="BsaI Binding Site"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          164..186
                     /label="JSpr454"
                     /note="sequence: cttcacGGTCTCgAGAGtcacactggctcaccttcg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          164..188
                     /label="JSpr463"
                     /note="sequence: tagagtcacactggctcaccttcggg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          167..184
                     /label="JSpr098"
                     /note="sequence: AGTCGAGGTCTCGTGgtcacactggctcacctt"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          167..186
                     /label="JSpr017"
                     /note="sequence: gtcacactggctcaccttcg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    175..202
                     /label="T7Te Terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(226..882)
                     /label="CmR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(234..255)
                     /label="JSpr428"
                     /note="sequence: CAGTACTGCGATGAGTGGCAGG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          241..264
                     /label="JSpr028"
                     /note="sequence: ctcatcgcagtactgttgtaattc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          361..380
                     /label="JSpr009"
                     /note="sequence: GCCCATGGTGAAAACGGGGG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(648..667)
                     /label="JSpr073"
                     /note="sequence: aggtctcgATTCCGTATGGCAATGAAAG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          662..683
                     /label="JSpr074"
                     /note="sequence: tggtctcgGAATTCCGGATGAGCATTCA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          679..700
                     /label="JSpr015"
                     /note="sequence: attcatcaggcgggcaagaatg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(839..864)
                     /label="JSpr029"
                     /note="sequence: ggatataccaccgttgatatatccca"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(883..985)
                     /label="Cat Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          890..918
                     /label="JSpr131"
                     /note="sequence: TCCTTAGCTCCTGAAAATCTCGATAACTC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(961..985)
                     /label="JSpr391"
                     /note="sequence: TGATCGGCACGTAAGAGGTTCCAAC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(964..985)
                     /label="JSpr101"
                     /note="sequence: cttcacggtctcgATTCCGTTGATCGGCACGTAAGAGGTTCC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(964..989)
                     /label="JSpr406"
                     /note="sequence: cttcacGGTCTCgCTAGTGATCGGCACGTAAGAGGTTCC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(968..985)
                     /label="JSpr094"
                     /note="sequence: tgatcggcacgtaagagg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(968..985)
                     /label="JSpr101"
                     /note="sequence: GTAAGGGGTCTCCTtgatcggcacgtaagagg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1035..1057
                     /label="JSpr448"
                     /note="sequence: cttcacGGTCTCgCTCTggcgtagaggatctgaagatcag"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(1043..1067)
                     /label="JSpr498"
                     /note="sequence: ggttgaactgctgatcttcagatcc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1044..1432
                     /label="R6K gamma oriR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication"
     primer          1326..1345
                     /label="JSpr446"
                     /note="sequence: taaggtttaacggttgtgga"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          1361..1385
                     /label="JSpr499"
                     /note="sequence: gtaacgcactgagaagcccttagag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1437..1442
                     /label="BsaI Binding Site"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     Promoter Swap   1444..1447
                     /label="GG Overhang"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1449..156
                     /label="BPI ES7"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 atagttacag cggttccaac gattcagcct taactggaac cgctgtaagc ctctcccaat
       61 ttttatatat tttatctatt tctcacttgc caaacaccat caatccgatg attttaaata
      121 gtcgaatcgt aattttatct ataatttgag gaagccATTA cGAGACCtca cactggctca
      181 ccttcgggtg ggcctttctg cgtttatacc taggagtcAA AATTACTCCC CGCCCTGCCA
      241 CTCATCGCAG TACTGTTGTA ATTCATTAAG CATTCTACCG ACATGGAAGC CATCACAAAC
      301 GGCATGATGA ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG TATAATATTT
      361 GCCCATGGTG AAAACGGGGG CGAAGAAGTT GTCCATATTG GCCACGTTTA AATCAAAACT
      421 GGTGAAACTC ACCCAGGGAT TGGCTGAGAC GAAAAACATA TTCTCAATAA ACCCTTTAGG
      481 GAAATAGGCC AGGTTTTCAC CGTAACACGC CACATCTTGC GAATATATGT GTAGAAACTG
      541 CCGGAAATCG TCGTGGTATT CACTCCAGAG CGATGAAAAC GTTTCAGTTT GCTCATGGAA
      601 AACGGTGTAA CAAGGGTGAA CACTATCCCA TATCACCAGC TCACCGTCTT TCATTGCCAT
      661 ACGGAATTCC GGATGAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG CCGGATAAAA
      721 CTTGTGCTTA TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA ATATCCAGCT GAACGGTCTG
      781 GTTATAGGTA CATTGAGCAA CTGACTGAAA TGCCTCAAAA TGTTCTTTAC GATGCCATTG
      841 GGATATATCA ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC
      901 TGAAAATCTC GATAACTCAA AAAATACGCC CGGTAGTGAT CTTATTTCAT TATGGTGAAA
      961 GTTGGAACCT CTTACGTGCC GATCAgtcaa ttcgaggggg atcaattccg tattgatccg
     1021 gccacgatgc gtccggcgta gaggatctga agatcagcag ttcaacctgt tgatagtacg
     1081 tactaagctc tcatgtttca cgtactaagc tctcatgttt aacgtactaa gctctcatgt
     1141 ttaacgaact aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc
     1201 tcatgtttca cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact
     1261 taaatagcct ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa
     1321 gcttttaagg tttaacggtt gtggacaaca agccagggat gtaacgcact gagaagccct
     1381 tagagcctct caaagcaatt ttgagtgaca caggaacact taacggctga catgggGGTC
     1441 TCgGAGActc ttaagatgac catgcgagct gccgcacatg cagctcgcgc gcctgcggct
     1501 gctttgattt taaagcattt ccagcaaaag tgggaacctc ctccgcaggg aaatcagttc
     1561 gccggactga tttcttttcc tgctgcgatg cgtcggataa tgcgtacaaa caa
//