LOCUS       pMRTK-clo34             1427 bp ds-DNA     circular     07-JUN-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          110..129
                     /label="JSpr009"
                     /note="sequence: GCCCATGGTGAAAACGGGGG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(397..416)
                     /label="JSpr073"
                     /note="sequence: aggtctcgATTCCGTATGGCAATGAAAG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          411..432
                     /label="JSpr074"
                     /note="sequence: tggtctcgGAATTCCGGATGAGCATTCA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          428..449
                     /label="JSpr015"
                     /note="sequence: attcatcaggcgggcaagaatg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(588..613)
                     /label="JSpr029"
                     /note="sequence: ggatataccaccgttgatatatccca"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(632..734)
                     /label="Cat Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          639..667
                     /label="JSpr131"
                     /note="sequence: TCCTTAGCTCCTGAAAATCTCGATAACTC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(710..734)
                     /label="JSpr391"
                     /note="sequence: TGATCGGCACGTAAGAGGTTCCAAC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(713..734)
                     /label="JSpr101"
                     /note="sequence: cttcacggtctcgATTCCGTTGATCGGCACGTAAGAGGTTCC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(713..738)
                     /label="JSpr406"
                     /note="sequence: cttcacGGTCTCgCTAGTGATCGGCACGTAAGAGGTTCC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(717..734)
                     /label="JSpr101"
                     /note="sequence: GTAAGGGGTCTCCTtgatcggcacgtaagagg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(717..734)
                     /label="JSpr094"
                     /note="sequence: tgatcggcacgtaagagg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          784..806
                     /label="JSpr448"
                     /note="sequence: cttcacGGTCTCgCTCTggcgtagaggatctgaagatcag"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(792..816)
                     /label="JSpr498"
                     /note="sequence: ggttgaactgctgatcttcagatcc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    793..1181
                     /label="R6K gamma oriR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication"
     primer          1075..1094
                     /label="JSpr446"
                     /note="sequence: taaggtttaacggttgtgga"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          1110..1134
                     /label="JSpr499"
                     /note="sequence: gtaacgcactgagaagcccttagag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1186..1191
                     /label="BsaI Binding Site"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     Promoter Swap   1193..1196
                     /label="GG Overhang"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1199..1332
                     /label="SMA ES1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1333..1336
                     /label="GG Site"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(1338..1343)
                     /label="BsaI Binding Site"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1340..1362
                     /label="JSpr454"
                     /note="sequence: cttcacGGTCTCgAGAGtcacactggctcaccttcg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          1340..1364
                     /label="JSpr463"
                     /note="sequence: tagagtcacactggctcaccttcggg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          1343..1360
                     /label="JSpr098"
                     /note="sequence: AGTCGAGGTCTCGTGgtcacactggctcacctt"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1343..1362
                     /label="JSpr017"
                     /note="sequence: gtcacactggctcaccttcg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1351..1378
                     /label="T7Te Terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(1402..631)
                     /label="CmR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1410..4)
                     /label="JSpr428"
                     /note="sequence: CAGTACTGCGATGAGTGGCAGG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1417..13
                     /label="JSpr028"
                     /note="sequence: ctcatcgcagtactgttgtaattc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
ORIGIN
        1 ACTGTTGTAA TTCATTAAGC ATTCTACCGA CATGGAAGCC ATCACAAACG GCATGATGAA
       61 CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG CCCATGGTGA
      121 AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG GTGAAACTCA
      181 CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG AAATAGGCCA
      241 GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC CGGAAATCGT
      301 CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA ACGGTGTAAC
      361 AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAATTCCG
      421 GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT
      481 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC
      541 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA
      601 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG
      661 ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAAG TTGGAACCTC
      721 TTACGTGCCG ATCAgtcaat tcgaggggga tcaattccgt attgatccgg ccacgatgcg
      781 tccggcgtag aggatctgaa gatcagcagt tcaacctgtt gatagtacgt actaagctct
      841 catgtttcac gtactaagct ctcatgttta acgtactaag ctctcatgtt taacgaacta
      901 aaccctcatg gctaacgtac taagctctca tggctaacgt actaagctct catgtttcac
      961 gtactaagct ctcatgtttg aacaataaaa ttaatataaa tcagcaactt aaatagcctc
     1021 taaggtttta agttttataa gaaaaaaaag aatatataag gcttttaaag cttttaaggt
     1081 ttaacggttg tggacaacaa gccagggatg taacgcactg agaagccctt agagcctctc
     1141 aaagcaattt tgagtgacac aggaacactt aacggctgac atgggGGTCT CgGAGAcggt
     1201 tttgcctgta tcatgcccgg ctcgacccac ccaggacggg tcgtacgccg atcatggcag
     1261 gacggaaaga gcggcccagg ccgccgccag atcagtcaga aaccagggtc atatcccatc
     1321 aaggacattg caATTAcGAG ACCtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1381 tatacctagg agtcAAAATT ACTCCCCGCC CTGCCACTCA TCGCAGT
//