LOCUS       pMRTK-clo35             1787 bp ds-DNA     circular     07-JUN-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(20..122)
                     /label="Cat Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          27..55
                     /label="JSpr131"
                     /note="sequence: TCCTTAGCTCCTGAAAATCTCGATAACTC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(98..122)
                     /label="JSpr391"
                     /note="sequence: TGATCGGCACGTAAGAGGTTCCAAC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(101..122)
                     /label="JSpr101"
                     /note="sequence: cttcacggtctcgATTCCGTTGATCGGCACGTAAGAGGTTCC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(101..126)
                     /label="JSpr406"
                     /note="sequence: cttcacGGTCTCgCTAGTGATCGGCACGTAAGAGGTTCC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(105..122)
                     /label="JSpr094"
                     /note="sequence: tgatcggcacgtaagagg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(105..122)
                     /label="JSpr101"
                     /note="sequence: GTAAGGGGTCTCCTtgatcggcacgtaagagg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          172..194
                     /label="JSpr448"
                     /note="sequence: cttcacGGTCTCgCTCTggcgtagaggatctgaagatcag"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(180..204)
                     /label="JSpr498"
                     /note="sequence: ggttgaactgctgatcttcagatcc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    181..569
                     /label="R6K gamma oriR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication"
     primer          463..482
                     /label="JSpr446"
                     /note="sequence: taaggtttaacggttgtgga"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          498..522
                     /label="JSpr499"
                     /note="sequence: gtaacgcactgagaagcccttagag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    574..579
                     /label="BsaI Binding Site"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     Promoter Swap   581..584
                     /label="GG Overhang"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    587..1080
                     /label="SMA ES2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1081..1084
                     /label="GG Site"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(1086..1091)
                     /label="BsaI Binding Site"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1088..1110
                     /label="JSpr454"
                     /note="sequence: cttcacGGTCTCgAGAGtcacactggctcaccttcg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          1088..1112
                     /label="JSpr463"
                     /note="sequence: tagagtcacactggctcaccttcggg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          1091..1108
                     /label="JSpr098"
                     /note="sequence: AGTCGAGGTCTCGTGgtcacactggctcacctt"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1091..1110
                     /label="JSpr017"
                     /note="sequence: gtcacactggctcaccttcg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1099..1126
                     /label="T7Te Terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(1150..19)
                     /label="CmR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1158..1179)
                     /label="JSpr428"
                     /note="sequence: CAGTACTGCGATGAGTGGCAGG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1165..1188
                     /label="JSpr028"
                     /note="sequence: ctcatcgcagtactgttgtaattc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1285..1304
                     /label="JSpr009"
                     /note="sequence: GCCCATGGTGAAAACGGGGG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1572..1591)
                     /label="JSpr073"
                     /note="sequence: aggtctcgATTCCGTATGGCAATGAAAG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          1586..1607
                     /label="JSpr074"
                     /note="sequence: tggtctcgGAATTCCGGATGAGCATTCA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1603..1624
                     /label="JSpr015"
                     /note="sequence: attcatcaggcgggcaagaatg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(1763..1)
                     /label="JSpr029"
                     /note="sequence: ggatataccaccgttgatatatccca"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA AAATCTCGAT AACTCAAAAA
       61 ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT GGAACCTCTT ACGTGCCGAT
      121 CAgtcaattc gagggggatc aattccgtat tgatccggcc acgatgcgtc cggcgtagag
      181 gatctgaaga tcagcagttc aacctgttga tagtacgtac taagctctca tgtttcacgt
      241 actaagctct catgtttaac gtactaagct ctcatgttta acgaactaaa ccctcatggc
      301 taacgtacta agctctcatg gctaacgtac taagctctca tgtttcacgt actaagctct
      361 catgtttgaa caataaaatt aatataaatc agcaacttaa atagcctcta aggttttaag
      421 ttttataaga aaaaaaagaa tatataaggc ttttaaagct tttaaggttt aacggttgtg
      481 gacaacaagc cagggatgta acgcactgag aagcccttag agcctctcaa agcaattttg
      541 agtgacacag gaacacttaa cggctgacat gggGGTCTCg GAGAcgattc cgaggccgaa
      601 gccatcctga atcgcgttca ggaaggtgtc gagaagccgc gtccgaaggt gctgttcgag
      661 ccgggccaga tggttcgcgt caccgacggc ccgttcaacg atttcaatgg cgtcgtcgaa
      721 gaagtcaact acgagaagag ccgtctgcgc gtctcggtgc tgatcttcgg tcgtgccact
      781 ccggtcgagc tcgagttcgg ccaggtcgaa aaggccgtct gaccggccct gtagagtcga
      841 gccatgctcg actcccgacc agaaaccggg ccttgcccgg tttcgttcgt ttatatgccg
      901 cttcaggcat gtgaagaaaa aacctgttat agtgcgcggc tccccgctgg gaagccagca
      961 ggacgaggcc gccgcaaggt ggccttcagc atgatttcaa aacgcgatgc cgggacgcgt
     1021 gattcccggt ccgatgggga gcctgttgtc gcaaggcgct agcacccgga gagcatactc
     1081 ATTAcGAGAC Ctcacactgg ctcaccttcg ggtgggcctt tctgcgttta tacctaggag
     1141 tcAAAATTAC TCCCCGCCCT GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT
     1201 ACCGACATGG AAGCCATCAC AAACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC
     1261 CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT
     1321 ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA
     1381 CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC
     1441 TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA
     1501 AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC
     1561 CAGCTCACCG TCTTTCATTG CCATACGGAA TTCCGGATGA GCATTCATCA GGCGGGCAAG
     1621 AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC
     1681 CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC
     1741 AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATC
//