LOCUS       HDM-tat1b               4829 bp    DNA     circular SYN  5-JAN-2008
DEFINITION  HIV tat 1b in HDM.
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to        4829)
  AUTHORS   GRAY JT
  TITLE     Direct Submission
  JOURNAL   Submitted (1-May-2003) John T. Gray
            Telephone: (617) 432-1249
            E-mail: gray@hihg.med.harvard.edu
            Harvard Gene Therapy Initiative
            Vector Core Lab, Room 412
            Harvard Institutes for Medicine
            77 Avenue Louis Pasteur
            Boston, MA 02115 USA
COMMENT     I (Jesse Bloom) received this plasmid from Alex Balazs to use as a helper plasmid
            for the PHAGE2 vectors.  It expresses Tat.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|470485875|
COMMENT     LSOWNER|
COMMENT     VNTNAME|HDM-tat1b|
COMMENT     VNTAUTHORNAME|Jesse Bloom|
COMMENT     VNTAUTHOREML|jesse.bloom@gmail.com|
COMMENT     VNTOAUTHORNAME|Alejandro Balazs|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "HIV tat 1b in HDM." 1) 
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COMMENT                          "Currently local object. Original author: Alejandro Balazs" 
COMMENT                          1) 
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COMMENT                                           "Extra EcoR I site of pMD-old-gag-pol.  This sequence is definitely not in all MDM constructs.  Probably only exists in a few older constructs prior to the MDM2b set.  This sequence has been found to be erroneous for some plasmids in our laboratory.  It would be prudent to sequence the DNA to confirm the accuracy of your records.  The sequence specified by this feature was actually found to be:  ACATGGCAGT CTAGAATTCT TGAAGACGAA AGGGCC." 
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COMMENT                                           "   3784..3827" 1))) "High_copy") 
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COMMENT                                           "Start: 4717  End: 4829" 1) 
COMMENT                                     #140=(CLinePar 
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COMMENT                                           "SV40 5172..41, contains origin of replication, from pMD cassette." 
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COMMENT                                     #142=(CLinePar 
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COMMENT                                           "   4717..4829" 1))) "SV40_ori"))) 
COMMENT                           "Misc. Feature (8 total)") 
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COMMENT                                           "Start: 1816  End: 1840" 1) 
COMMENT                                     #146=(CLinePar 
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COMMENT                                           "Axxs added after these bases in globin message." 
COMMENT                                           1) 
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COMMENT                                     #148=(CLinePar 
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COMMENT                                           "   1816..1840" 1))) "polyA_site"))) 
COMMENT                           "PolyA Site (1 total)") 
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COMMENT                                           "Start: 151   End: 178 " 1) 
COMMENT                                     #152=(CLinePar 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 638 bases.  Perfect match Tm = 58.7 C, although only 17 nt are required to match.  The actual sequence of the primer is agttccgcgt tacataactt acggtaaa." 
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COMMENT                                           "   151..178" 1))) 
COMMENT                                   "HGTI-1832_Max=638") 
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COMMENT                                           "Start: 236   End: 260  (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #157=(CLinePar 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 672 bases.  Perfect match Tm = 59.1 C, although only 17 nt are required to match.  The actual sequence of the primer is ccctattggc gttactatgg gaaca." 
COMMENT                                           1) 
COMMENT                                     #158=(CLinePar 
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COMMENT                                     #159=(CLinePar 
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COMMENT                                           "   complement(236..260)" 1))) 
COMMENT                                   "HGTI-1863_Max=672") 
COMMENT                             #160=(CFolderPar 
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COMMENT                                           "Start: 536   End: 560  (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #162=(CLinePar 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 485 bases.  Perfect match Tm = 58.0 C, although only 17 nt are required to match.  The actual sequence of the primer is aaacaaactc ccattgacgt caatg." 
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COMMENT                                     #163=(CLinePar 
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COMMENT                                     #164=(CLinePar 
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COMMENT                                           "   complement(536..560)" 1))) 
COMMENT                                   "HGTI-1864_Max=485") 
COMMENT                             #165=(CFolderPar 
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COMMENT                                           "Start: 561   End: 593 " 1) 
COMMENT                                     #167=(CLinePar 
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COMMENT                                           "sequencing pHD from 3xx end  Max recorded sequencing read = 707 bases. Perfect match Tm = 71.5 C, although only 17 nt are required to match.  The actual sequence of the primer is tggcaccaaa atcaacggga ctttccaaaa tgt." 
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COMMENT                                     #168=(CLinePar 
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COMMENT                                     #169=(CLinePar 
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COMMENT                                           "   561..593" 1))) 
COMMENT                                   "HGTI-2021_Max=707") 
COMMENT                             #170=(CFolderPar 
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COMMENT                                     #171=(CLinePar 
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COMMENT                                           "Start: 612   End: 652 " 1) 
COMMENT                                     #172=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "?  Max recorded sequencing read = 162 bases.  Perfect match Tm = 76.1 C, although only 17 nt are required to match.  The actual sequence of the primer is gcgcactagt catatggtag gcgtgtacgg tgggaggtct a." 
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COMMENT                                     #174=(CLinePar 
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COMMENT                                           "   612..652" 1))) 
COMMENT                                   "HGTI-1220_Max=162") 
COMMENT                             #175=(CFolderPar 
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COMMENT                                           "Start: 617   End: 638 " 1) 
COMMENT                                     #177=(CLinePar 
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COMMENT                                           "Sequencing primers for pHJ1  Max recorded sequencing read = 311 bases. Perfect match Tm = 62.5 C, although only 17 nt are required to match.  The actual sequence of the primer is GCAAATGGGC GGTAGGCGTG TA." 
COMMENT                                           1) 
COMMENT                                     #178=(CLinePar 
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COMMENT                                     #179=(CLinePar 
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COMMENT                                           "   617..638" 1))) 
COMMENT                                   "HGTI-1204_Max=311") 
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COMMENT                                           "Start: 662   End: 703  (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #182=(CLinePar 
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COMMENT                                           "linker from SacI of CMV bridging into BglII of HIV R.  Max recorded sequencing read =  bases.  Perfect match Tm = 74.8 C, although only 17 nt are required to match.  The actual sequence of the primer is gatcTGGTCT AACCAGAGAG ACCCcggttc actaaacgAG CT." 
COMMENT                                           1) 
COMMENT                                     #183=(CLinePar 
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COMMENT                                     #184=(CLinePar 
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COMMENT                                           "   complement(662..703)" 1))) 
COMMENT                                   "HGTI-1192_Max=") 
COMMENT                             #185=(CFolderPar 
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COMMENT                                           "Start: 692   End: 715 " 1) 
COMMENT                                     #187=(CLinePar 
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COMMENT                                           "PCR or sequencing primer located in CMV sequence just after 5xx end of RNA transcript.  Max recorded sequencing read =  bases.  Perfect match Tm = 63.9 C, although only 17 nt are required to match.  The actual sequence of the primer is GAGACGCCAT CCACGCTGTT TTGA." 
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COMMENT                                     #188=(CLinePar 
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COMMENT                                     #189=(CLinePar 
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COMMENT                                           "   692..715" 1))) "HGTI-761_Max=") 
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COMMENT                                           "Start: 751   End: 775 " 1) 
COMMENT                                     #192=(CLinePar 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 595 bases.  Perfect match Tm = 58.7 C, although only 17 nt are required to match.  The actual sequence of the primer is cctcgaagct gatcctgaga acttc." 
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COMMENT                                     #194=(CLinePar 
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COMMENT                                           "   751..775" 1))) 
COMMENT                                   "HGTI-1834_Max=595") 
COMMENT                             #195=(CFolderPar 
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COMMENT                                           "Start: 833   End: 860  (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #197=(CLinePar 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 636 bases.  Perfect match Tm = 59.3 C, although only 17 nt are required to match.  The actual sequence of the primer is tgttacttct ccccttccta tgacatga." 
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COMMENT                                     #198=(CLinePar 
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COMMENT                                     #199=(CLinePar 
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COMMENT                                           "   complement(833..860)" 1))) 
COMMENT                                   "HGTI-1865_Max=636") 
COMMENT                             #200=(CFolderPar 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 952   End: 981  (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #202=(CLinePar 
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COMMENT                                           "sequencing primer in globin intron facing left.  Max recorded sequencing read = 610 bases.  Perfect match Tm = 53.9 C, although only 17 nt are required to match.  The actual sequence of the primer is gaaagaaaga gattagggaa agtattagaa ." 
COMMENT                                           1) 
COMMENT                                     #203=(CLinePar 
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COMMENT                                     #204=(CLinePar 
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COMMENT                                           "   complement(952..981)" 1))) 
COMMENT                                   "HGTI-2022_Max=610") 
COMMENT                             #205=(CFolderPar 
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COMMENT                                     #206=(CLinePar 
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COMMENT                                           "Start: 1051  End: 1082" 1) 
COMMENT                                     #207=(CLinePar 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 639 bases.  Perfect match Tm = 58.2 C, although only 17 nt are required to match.  The actual sequence of the primer is gttaaggcaa tagcaatatt tctgcatata aa." 
COMMENT                                           1) 
COMMENT                                     #208=(CLinePar 
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COMMENT                                           "   1051..1082" 1))) 
COMMENT                                   "HGTI-1835_Max=639") 
COMMENT                             #210=(CFolderPar 
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COMMENT                                           "Start: 1135  End: 1160 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #212=(CLinePar 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 639 bases.  Perfect match Tm = 59.3 C, although only 17 nt are required to match.  The actual sequence of the primer is gcagaatggt agctggattg tagctg." 
COMMENT                                           1) 
COMMENT                                     #213=(CLinePar 
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COMMENT                                     #214=(CLinePar 
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COMMENT                                           "   complement(1135..1160)" 1))) 
COMMENT                                   "HGTI-1866_Max=639") 
COMMENT                             #215=(CFolderPar 
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COMMENT                                           "Start: 1209  End: 1243" 1) 
COMMENT                                     #217=(CLinePar 
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COMMENT                                           "New 5xx sequencing primer for B-globin constructs.  Same position as JSL-70, but longer with a Tm at 66C.  Max recorded sequencing read = 783 bases.  Perfect match Tm = 65.5 C, although only 17 nt are required to match. The actual sequence of the primer is GGCCCTTTTG CTAATCATGT TCATACCTCT TATCT." 
COMMENT                                           1) 
COMMENT                                     #218=(CLinePar 
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COMMENT                                     #219=(CLinePar 
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COMMENT                                           "   1209..1243" 1))) 
COMMENT                                   "HGTI-1732_Max=783") 
COMMENT                             #220=(CFolderPar 
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COMMENT                                           "Start: 1224  End: 1244" 1) 
COMMENT                                     #222=(CLinePar 
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COMMENT                                           "Jeng Shin designed sequencing primer @ 5xx end of MDM2b polylinker facing towards gene.  xx723 is longer, higher Tm versionxx.  Max recorded sequencing read = 0 bases.  Perfect match Tm = 40.3 C, although only 17 nt are required to match.  The actual sequence of the primer is CATGTTCATA CCTCTTATCT T." 
COMMENT                                           1) 
COMMENT                                     #223=(CLinePar 
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COMMENT                                     #224=(CLinePar 
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COMMENT                                           "   1224..1244" 1))) 
COMMENT                                   "HGTI-1278_Max=0") 
COMMENT                             #225=(CFolderPar 
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COMMENT                                           "Start: 1226  End: 1253" 1) 
COMMENT                                     #227=(CLinePar 
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COMMENT                                           "sFlt clone Add-musFlt-mIg: sequencing ORF  Max recorded sequencing read = 0 bases.  Perfect match Tm = 59.3 C, although only 17 nt are required to match.  The actual sequence of the primer is TGTTCATACC TCTTATCTTC CTCCCACA." 
COMMENT                                           1) 
COMMENT                                     #228=(CLinePar 
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COMMENT                                     #229=(CLinePar 
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COMMENT                                           "   1226..1253" 1))) 
COMMENT                                   "HGTI-2144_Max=0") 
COMMENT                             #230=(CFolderPar 
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COMMENT                                           "Start: 1473  End: 1497 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #232=(CLinePar 
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COMMENT                                           "Sequencing primers for HIV strain HXB2 construct.  Max recorded sequencing read =  bases.  Perfect match Tm = 64.9 C, although only 17 nt are required to match.  The actual sequence of the primer is GCTCTTCGTC GCTGTCTCCG CTTCT." 
COMMENT                                           1) 
COMMENT                                     #233=(CLinePar 
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COMMENT                                     #234=(CLinePar 
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COMMENT                                           "   complement(1473..1497)" 1))) 
COMMENT                                   "HGTI-1121_Max=") 
COMMENT                             #235=(CFolderPar 
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COMMENT                                           "Start: 1675  End: 1692 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #237=(CLinePar 
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COMMENT                                           "Jeng Shin designed sequencing primer @ 3xx end of MDM2b polylinker facing towards gene.New incarnation is 3149, reordered 1xx29xx01.  Max recorded sequencing read =  bases.  Perfect match Tm = 54.2 C, although only 17 nt are required to match.  The actual sequence of the primer is gccagggcat tagccaca." 
COMMENT                                           1) 
COMMENT                                     #238=(CLinePar 
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COMMENT                                     #239=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(1675..1692)" 1))) 
COMMENT                                   "HGTI-1279_Max=") 
COMMENT                             #240=(CFolderPar 
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COMMENT                                    (CParagraph 1798 (3 #35# 0) 1 2 2 0 194) 
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COMMENT                                           "Start: 1798  End: 1826" 1) 
COMMENT                                     #242=(CLinePar 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 379 bases.  Perfect match Tm = 59.8 C, although only 17 nt are required to match.  The actual sequence of the primer is agcatctgga ttctgcctaa taaaaaaca." 
COMMENT                                           1) 
COMMENT                                     #243=(CLinePar 
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COMMENT                                           "   1798..1826" 1))) 
COMMENT                                   "HGTI-1843_Max=379") 
COMMENT                             #245=(CFolderPar 
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COMMENT                                           "Start: 1883  End: 1907 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #247=(CLinePar 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 692 bases.  Perfect match Tm = 58.1 C, although only 17 nt are required to match.  The actual sequence of the primer is ttaaatgcac tgacctccca cattc." 
COMMENT                                           1) 
COMMENT                                     #248=(CLinePar 
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COMMENT                                     #249=(CLinePar 
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COMMENT                                           "   complement(1883..1907)" 1))) 
COMMENT                                   "HGTI-1874_Max=692") 
COMMENT                             #250=(CFolderPar 
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COMMENT                                           "Start: 2099  End: 2132" 1) 
COMMENT                                     #252=(CLinePar 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 643 bases.  Perfect match Tm = 59.1 C, although only 17 nt are required to match.  The actual sequence of the primer is ttttacatta cttattgttt tagctgtcct catg." 
COMMENT                                           1) 
COMMENT                                     #253=(CLinePar 
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COMMENT                                     #254=(CLinePar 
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COMMENT                                           "   2099..2132" 1))) 
COMMENT                                   "HGTI-1844_Max=643") 
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COMMENT                                           "Start: 2178  End: 2208 (Complementary)" 
COMMENT                                           1) 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 610 bases.  Perfect match Tm = 59.5 C, although only 17 nt are required to match.  The actual sequence of the primer is tatctctaag caagagaact gagtggagtc a." 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 711 bases.  Perfect match Tm = 58.3 C, although only 17 nt are required to match.  The actual sequence of the primer is gacattaacc tataaaaata ggcgtatcac g." 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 647 bases.  Perfect match Tm = 60.2 C, although only 17 nt are required to match.  The actual sequence of the primer is ccttttttgc ggcattttgc c." 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 661 bases.  Perfect match Tm = 58.0 C, although only 17 nt are required to match.  The actual sequence of the primer is atccagttcg atgtaaccca ctcg." 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 654 bases.  Perfect match Tm = 60.0 C, although only 17 nt are required to match.  The actual sequence of the primer is cggatggcat gacagtaaga gaattatg." 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 650 bases.  Perfect match Tm = 58.4 C, although only 17 nt are required to match.  The actual sequence of the primer is agcggttagc tccttcggtc c." 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 593 bases.  Perfect match Tm = 59.6 C, although only 17 nt are required to match.  The actual sequence of the primer is ataaagttgc aggaccactt ctgcg." 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 0 bases.  Perfect match Tm = 61.0 C, although only 17 nt are required to match.  The actual sequence of the primer is ccccagtgct gcaatgatac cg." 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 564 bases.  Perfect match Tm = 58.2 C, although only 17 nt are required to match.  The actual sequence of the primer is tgaagatcct ttttgataat ctcatgacc." 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 372 bases.  Perfect match Tm = 58.8 C, although only 17 nt are required to match.  The actual sequence of the primer is aaaaggatct caagaagatc ctttgatctt ." 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 565 bases.  Perfect match Tm = 60.5 C, although only 17 nt are required to match.  The actual sequence of the primer is catacctcgc tctgctaatc ctgttacc." 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 601 bases.  Perfect match Tm = 60.1 C, although only 17 nt are required to match.  The actual sequence of the primer is cgaccgctgc gccttatcc." 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 511 bases.  Perfect match Tm = 59 C, although only 17 nt are required to match.  The actual sequence of the primer is atctttatag tcctgtcggg tttcgc." 
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COMMENT                                           "Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 662 bases.  Perfect match Tm = 60.7 C, although only 17 nt are required to match.  The actual sequence of the primer is agcgagaaga atcataatgg ggaagg." 
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COMMENT                                           "Add-musFLT-mIg Plasmid record #912  Max recorded sequencing read = 618 bases.  Perfect match Tm = 59.3 C, although only 17 nt are required to match. The actual sequence of the primer is tccagaagta gtgaggaggc ttttttg." 
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COMMENT                                           "CMV promoter.  Transcription starts with next base.  5 prime end represents complete extent of the CMV promoter in MDM based plasmids, which corresponds to positions 471..1141 of hCMVie genbank record accession X03922." 
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FEATURES             Location/Qualifiers
     misc_feature    779..1254
                     /vntifkey="21"
                     /label=B-globin_intron
                     /note="Human B-globin 2nd intron, Rsa I deleted."
     polyA_site      1816..1840
                     /vntifkey="26"
                     /label=polyA_site
                     /note="Axxs added after these bases in globin message."
     misc_feature    2364..2388
                     /vntifkey="21"
                     /label=End_of_globin
                     /note="End of globin sequences in typical pMD constructs."
     CDS             2628..3485
                     /vntifkey="4"
                     /label=bla
                     /note="B-lactamase, confers ampicillin resistance, no Pst I."
     misc_feature    628..679
                     /vntifkey="21"
                     /label=CMV_TATA
                     /note="CMV TATA box sequences, from -52 to -1.  Wild type, with Sac I site."
     misc_feature    4717..4829
                     /vntifkey="21"
                     /label=SV40_ori
                     /note="SV40 5172..41, contains origin of replication, from pMD cassette."
     misc_feature    3784..3827
                     /vntifkey="21"
                     /label=High_copy
                     /note="Tag for High copy plasmid replication xxpUC18xx"
     promoter        9..679
                     /vntifkey="30"
                     /label=CMV
                     /note="CMV promoter.  Transcription starts with next base.  5 prime end represents complete extent of the CMV promoter in MDM based plasmids, which corresponds to positions 471..1141 of hCMVie genbank record accession X03922."
     misc_feature    680..750
                     /vntifkey="21"
                     /label=CMV_5xxUTR
                     /note="5xx untranslated region from CMV immediate early promoter.  The 3 prime end corresponds to the full extent of CMV sequences in the MDM constructs."
     misc_feature    1629..2388
                     /vntifkey="21"
                     /label=beta_globin_pA
                     /note="From 3rd exon of human beta globin, contains polyA sequences commonly used in HGTI vector lab constructs."
     promoter        96..679
                     /vntifkey="30"
                     /label=CMV
                     /note="CMV promoter.  Transcription starts with next base.  5 prime end starts at Spe I site only.  The CMV region in this construct may extend further upstream."
     misc_feature    2402..2439
                     /vntifkey="21"
                     /label=Correction-143
                     /note="Extra EcoR I site of pMD-old-gag-pol.  This sequence is definitely not in all MDM constructs.  Probably only exists in a few older constructs prior to the MDM2b set.  This sequence has been found to be erroneous for some plasmids in our laboratory.  It would be prudent to sequence the DNA to confirm the accuracy of your records.  The sequence specified by this feature was actually found to be:  ACATGGCAGT CTAGAATTCT TGAAGACGAA AGGGCC."
     primer_bind     complement(4742..4768)
                     /vntifkey="28"
                     /label=HGTI-8_Max=618
                     /note="Add-musFLT-mIg Plasmid record #912  Max recorded sequencing read = 618 bases.  Perfect match Tm = 59.3 C, although only 17 nt are required to match. The actual sequence of the primer is tccagaagta gtgaggaggc ttttttg."
     primer_bind     692..715
                     /vntifkey="28"
                     /label=HGTI-761_Max=
                     /note="PCR or sequencing primer located in CMV sequence just after 5xx end of RNA transcript.  Max recorded sequencing read =  bases.  Perfect match Tm = 63.9 C, although only 17 nt are required to match.  The actual sequence of the primer is GAGACGCCAT CCACGCTGTT TTGA."
     primer_bind     complement(1473..1497)
                     /vntifkey="28"
                     /label=HGTI-1121_Max=
                     /note="Sequencing primers for HIV strain HXB2 construct.  Max recorded sequencing read =  bases.  Perfect match Tm = 64.9 C, although only 17 nt are required to match.  The actual sequence of the primer is GCTCTTCGTC GCTGTCTCCG CTTCT."
     primer_bind     complement(662..703)
                     /vntifkey="28"
                     /label=HGTI-1192_Max=
                     /note="linker from SacI of CMV bridging into BglII of HIV R.  Max recorded sequencing read =  bases.  Perfect match Tm = 74.8 C, although only 17 nt are required to match.  The actual sequence of the primer is gatcTGGTCT AACCAGAGAG ACCCcggttc actaaacgAG CT."
     primer_bind     617..638
                     /vntifkey="28"
                     /label=HGTI-1204_Max=311
                     /note="Sequencing primers for pHJ1  Max recorded sequencing read = 311 bases. Perfect match Tm = 62.5 C, although only 17 nt are required to match.  The actual sequence of the primer is GCAAATGGGC GGTAGGCGTG TA."
     primer_bind     612..652
                     /vntifkey="28"
                     /label=HGTI-1220_Max=162
                     /note="?  Max recorded sequencing read = 162 bases.  Perfect match Tm = 76.1 C, although only 17 nt are required to match.  The actual sequence of the primer is gcgcactagt catatggtag gcgtgtacgg tgggaggtct a."
     primer_bind     1224..1244
                     /vntifkey="28"
                     /label=HGTI-1278_Max=0
                     /note="Jeng Shin designed sequencing primer @ 5xx end of MDM2b polylinker facing towards gene.  xx723 is longer, higher Tm versionxx.  Max recorded sequencing read = 0 bases.  Perfect match Tm = 40.3 C, although only 17 nt are required to match.  The actual sequence of the primer is CATGTTCATA CCTCTTATCT T."
     primer_bind     complement(1675..1692)
                     /vntifkey="28"
                     /label=HGTI-1279_Max=
                     /note="Jeng Shin designed sequencing primer @ 3xx end of MDM2b polylinker facing towards gene.New incarnation is 3149, reordered 1xx29xx01.  Max recorded sequencing read =  bases.  Perfect match Tm = 54.2 C, although only 17 nt are required to match.  The actual sequence of the primer is gccagggcat tagccaca."
     primer_bind     1209..1243
                     /vntifkey="28"
                     /label=HGTI-1732_Max=783
                     /note="New 5xx sequencing primer for B-globin constructs.  Same position as JSL-70, but longer with a Tm at 66C.  Max recorded sequencing read = 783 bases.  Perfect match Tm = 65.5 C, although only 17 nt are required to match. The actual sequence of the primer is GGCCCTTTTG CTAATCATGT TCATACCTCT TATCT."
     primer_bind     4670..4695
                     /vntifkey="28"
                     /label=HGTI-1828_Max=662
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 662 bases.  Perfect match Tm = 60.7 C, although only 17 nt are required to match.  The actual sequence of the primer is agcgagaaga atcataatgg ggaagg."
     primer_bind     151..178
                     /vntifkey="28"
                     /label=HGTI-1832_Max=638
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 638 bases.  Perfect match Tm = 58.7 C, although only 17 nt are required to match.  The actual sequence of the primer is agttccgcgt tacataactt acggtaaa."
     primer_bind     751..775
                     /vntifkey="28"
                     /label=HGTI-1834_Max=595
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 595 bases.  Perfect match Tm = 58.7 C, although only 17 nt are required to match.  The actual sequence of the primer is cctcgaagct gatcctgaga acttc."
     primer_bind     1051..1082
                     /vntifkey="28"
                     /label=HGTI-1835_Max=639
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 639 bases.  Perfect match Tm = 58.2 C, although only 17 nt are required to match.  The actual sequence of the primer is gttaaggcaa tagcaatatt tctgcatata aa."
     primer_bind     1798..1826
                     /vntifkey="28"
                     /label=HGTI-1843_Max=379
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 379 bases.  Perfect match Tm = 59.8 C, although only 17 nt are required to match.  The actual sequence of the primer is agcatctgga ttctgcctaa taaaaaaca."
     primer_bind     2099..2132
                     /vntifkey="28"
                     /label=HGTI-1844_Max=643
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 643 bases.  Perfect match Tm = 59.1 C, although only 17 nt are required to match.  The actual sequence of the primer is ttttacatta cttattgttt tagctgtcct catg."
     primer_bind     complement(3953..3971)
                     /vntifkey="28"
                     /label=HGTI-1851_Max=601
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 601 bases.  Perfect match Tm = 60.1 C, although only 17 nt are required to match.  The actual sequence of the primer is cgaccgctgc gccttatcc."
     primer_bind     complement(3642..3671)
                     /vntifkey="28"
                     /label=HGTI-1852_Max=372
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 372 bases.  Perfect match Tm = 58.8 C, although only 17 nt are required to match.  The actual sequence of the primer is aaaaggatct caagaagatc ctttgatctt ."
     primer_bind     complement(3350..3371)
                     /vntifkey="28"
                     /label=HGTI-1853_Max=0
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 0 bases.  Perfect match Tm = 61.0 C, although only 17 nt are required to match.  The actual sequence of the primer is ccccagtgct gcaatgatac cg."
     primer_bind     complement(3051..3071)
                     /vntifkey="28"
                     /label=HGTI-1854_Max=650
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 650 bases.  Perfect match Tm = 58.4 C, although only 17 nt are required to match.  The actual sequence of the primer is agcggttagc tccttcggtc c."
     primer_bind     complement(2748..2771)
                     /vntifkey="28"
                     /label=HGTI-1855_Max=661
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 661 bases.  Perfect match Tm = 58.0 C, although only 17 nt are required to match.  The actual sequence of the primer is atccagttcg atgtaaccca ctcg."
     primer_bind     complement(2441..2471)
                     /vntifkey="28"
                     /label=HGTI-1856_Max=711
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 711 bases.  Perfect match Tm = 58.3 C, although only 17 nt are required to match.  The actual sequence of the primer is gacattaacc tataaaaata ggcgtatcac g."
     primer_bind     complement(236..260)
                     /vntifkey="28"
                     /label=HGTI-1863_Max=672
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 672 bases.  Perfect match Tm = 59.1 C, although only 17 nt are required to match.  The actual sequence of the primer is ccctattggc gttactatgg gaaca."
     primer_bind     complement(536..560)
                     /vntifkey="28"
                     /label=HGTI-1864_Max=485
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 485 bases.  Perfect match Tm = 58.0 C, although only 17 nt are required to match.  The actual sequence of the primer is aaacaaactc ccattgacgt caatg."
     primer_bind     complement(833..860)
                     /vntifkey="28"
                     /label=HGTI-1865_Max=636
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 636 bases.  Perfect match Tm = 59.3 C, although only 17 nt are required to match.  The actual sequence of the primer is tgttacttct ccccttccta tgacatga."
     primer_bind     complement(1135..1160)
                     /vntifkey="28"
                     /label=HGTI-1866_Max=639
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 639 bases.  Perfect match Tm = 59.3 C, although only 17 nt are required to match.  The actual sequence of the primer is gcagaatggt agctggattg tagctg."
     primer_bind     complement(1883..1907)
                     /vntifkey="28"
                     /label=HGTI-1874_Max=692
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 692 bases.  Perfect match Tm = 58.1 C, although only 17 nt are required to match.  The actual sequence of the primer is ttaaatgcac tgacctccca cattc."
     primer_bind     complement(2178..2208)
                     /vntifkey="28"
                     /label=HGTI-1875_Max=610
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 610 bases.  Perfect match Tm = 59.5 C, although only 17 nt are required to match.  The actual sequence of the primer is tatctctaag caagagaact gagtggagtc a."
     primer_bind     4162..4187
                     /vntifkey="28"
                     /label=HGTI-1881_Max=511
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 511 bases.  Perfect match Tm = 59 C, although only 17 nt are required to match.  The actual sequence of the primer is atctttatag tcctgtcggg tttcgc."
     primer_bind     3862..3889
                     /vntifkey="28"
                     /label=HGTI-1882_Max=565
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 565 bases.  Perfect match Tm = 60.5 C, although only 17 nt are required to match.  The actual sequence of the primer is catacctcgc tctgctaatc ctgttacc."
     primer_bind     3562..3590
                     /vntifkey="28"
                     /label=HGTI-1883_Max=564
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 564 bases.  Perfect match Tm = 58.2 C, although only 17 nt are required to match.  The actual sequence of the primer is tgaagatcct ttttgataat ctcatgacc."
     primer_bind     3262..3286
                     /vntifkey="28"
                     /label=HGTI-1884_Max=593
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 593 bases.  Perfect match Tm = 59.6 C, although only 17 nt are required to match.  The actual sequence of the primer is ataaagttgc aggaccactt ctgcg."
     primer_bind     2962..2989
                     /vntifkey="28"
                     /label=HGTI-1885_Max=654
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 654 bases.  Perfect match Tm = 60.0 C, although only 17 nt are required to match.  The actual sequence of the primer is cggatggcat gacagtaaga gaattatg."
     primer_bind     2662..2682
                     /vntifkey="28"
                     /label=HGTI-1886_Max=647
                     /note="Full plasmid sequencing for Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 647 bases.  Perfect match Tm = 60.2 C, although only 17 nt are required to match.  The actual sequence of the primer is ccttttttgc ggcattttgc c."
     primer_bind     561..593
                     /vntifkey="28"
                     /label=HGTI-2021_Max=707
                     /note="sequencing pHD from 3xx end  Max recorded sequencing read = 707 bases. Perfect match Tm = 71.5 C, although only 17 nt are required to match.  The actual sequence of the primer is tggcaccaaa atcaacggga ctttccaaaa tgt."
     primer_bind     complement(952..981)
                     /vntifkey="28"
                     /label=HGTI-2022_Max=610
                     /note="sequencing primer in globin intron facing left.  Max recorded sequencing read = 610 bases.  Perfect match Tm = 53.9 C, although only 17 nt are required to match.  The actual sequence of the primer is gaaagaaaga gattagggaa agtattagaa ."
     primer_bind     1226..1253
                     /vntifkey="28"
                     /label=HGTI-2144_Max=0
                     /note="sFlt clone Add-musFlt-mIg: sequencing ORF  Max recorded sequencing read = 0 bases.  Perfect match Tm = 59.3 C, although only 17 nt are required to match.  The actual sequence of the primer is TGTTCATACC TCTTATCTTC CTCCCACA."
     CDS             1325..1585
                     /vntifkey="4"
                     /label=Tat1b
                     /note="HIV Tat Protein"
BASE COUNT     1239 a      1153 c      1137 g      1300 t 
ORIGIN
        1 agcttggccc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat 
       61 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta 
      121 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 
      721 atagaagaca ccgggaccga tccagcctcc cctcgaagct gatcctgaga acttcagggt 
      781 gagtctatgg gacccttgat gttttctttc cccttctttt ctatggttaa gttcatgtca 
      841 taggaagggg agaagtaaca gggtacacat attgaccaaa tcagggtaat tttgcatttg 
      901 taattttaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac 
      961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc 
     1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata 
     1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta 
     1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt 
     1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg 
     1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattccg cgggcggccg 
     1321 cgaaatggag ccagtagatc ctagactaga gccctggaag catccaggaa gtcagcctaa 
     1381 aactgcttgt accacttgct attgtaaaaa gtgttgcttt cattgccaag tttgtttcac 
     1441 aacaaaagcc ttaggcatct cctatggcag gaagaagcgg agacagcgac gaagacctcc 
     1501 tcaaggcagt cagactcatc aagtttctct atcaaagcaa cccacctccc aaccccgagg 
     1561 ggacccgaca ggcccgaagg aataggatcc aagcttatcg ataccgtcga cctcgagggc 
     1621 ccagatctaa ttcaccccac cagtgcaggc tgcctatcag aaagtggtgg ctggtgtggc 
     1681 taatgccctg gcccacaagt atcactaagc tcgctttctt gctgtccaat ttctattaaa 
     1741 ggttcctttg ttccctaagt ccaactacta aactggggga tattatgaag ggccttgagc 
     1801 atctggattc tgcctaataa aaaacattta ttttcattgc aatgatgtat ttaaattatt 
     1861 tctgaatatt ttactaaaaa gggaatgtgg gaggtcagtg catttaaaac ataaagaaat 
     1921 gaagagctag ttcaaacctt gggaaaatac actatatctt aaactccatg aaagaaggtg 
     1981 aggctgcaaa cagctaatgc acattggcaa cagcccctga tgcctatgcc ttattcatcc 
     2041 ctcagaaaag gattcaagta gaggcttgat ttggaggtta aagttttgct atgctgtatt 
     2101 ttacattact tattgtttta gctgtcctca tgaatgtctt ttcactaccc atttgcttat 
     2161 cctgcatctc tcagccttga ctccactcag ttctcttgct tagagatacc acctttcccc 
     2221 tgaagtgttc cttccatgtt ttacggcgag atggtttctc ctcgcctggc cactcagcct 
     2281 tagttgtctc tgttgtctta tagaggtcta cttgaagaag gaaaaacagg gggcatggtt 
     2341 tgactgtcct gtgagccctt cttccctgcc tcccccactc acagtgaccc ggaatccctc 
     2401 gacatggcag tctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat 
     2461 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 
     2521 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 
     2581 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 
     2641 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 
     2701 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 
     2761 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 
     2821 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 
     2881 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 
     2941 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 
     3001 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 
     3061 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 
     3121 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 
     3181 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 
     3241 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 
     3301 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 
     3361 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 
     3421 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 
     3481 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 
     3541 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 
     3601 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 
     3661 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 
     3721 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 
     3781 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 
     3841 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 
     3901 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 
     3961 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 
     4021 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 
     4081 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 
     4141 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 
     4201 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 
     4261 gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg 
     4321 ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg 
     4381 aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac 
     4441 cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc 
     4501 cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccaatgatcg 
     4561 aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta 
     4621 cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa 
     4681 tcataatggg gaaggccatc cagcctcgcg tcggggagct ttttgcaaaa gcctaggcct 
     4741 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct 
     4801 gcataaataa aaaaaattag tcagccatg 
//
