LOCUS       2850_pHAGE2_EF1a        9026 bp    DNA        circular     17-AUG-2020
DEFINITION  .
ACCESSION   Your (modified)                                                    
SOURCE      Unknown                                                            
  ORGANISM  Unknown Unclassified.                                              
COMMENT     This plasmid was created by Caelan Radford. 
COMMENT     
COMMENT     This plasmid was made by digesting the SarsCov2 Spike sequence out
            of plasmid 2715 with NotI and BamHI and replacing it with the rtTA
            (TetOn) gene amplified from plasmid 2454 (pCAG-TetOn). It
            constitutively expresses rtTA and mCherry. It was sequence
            confirmed using Sanger sequencing in May 2020. 
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     LTR             1..636
                     /locus_tag="5' LTR"
                     /label="5' LTR"
                     /ApEinfo_label="5' LTR"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(8180..8201)
                     /locus_tag="pHAGE-A7550-7050"
                     /label="pHAGE-A7550-7050"
                     /ApEinfo_label="pHAGE-A7550-7050"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     stem_loop       766..779
                     /locus_tag="SL-3"
                     /label="SL-3"
                     /ApEinfo_label="SL-3"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(667..690)
                     /locus_tag="pHAGE-A0550-0050"
                     /label="pHAGE-A0550-0050"
                     /ApEinfo_label="pHAGE-A0550-0050"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      7708..8241
                     /locus_tag="ORI"
                     /label="ORI"
                     /ApEinfo_label="ORI"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             790..1144
                     /locus_tag="HIV gag"
                     /label="HIV gag"
                     /ApEinfo_label="HIV gag"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     5'UTR           551..635
                     /locus_tag="5' UTR"
                     /label="5' UTR"
                     /ApEinfo_label="5' UTR"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     8921..8949
                     /locus_tag="pHAGE-S1-500"
                     /label="pHAGE-S1-500"
                     /ApEinfo_label="pHAGE-S1-500"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(4189..4209)
                     /locus_tag="pHAGE 3' CDS 1-1 (Binds IRES)"
                     /label="pHAGE 3' CDS 1-1 (Binds IRES)"
                     /ApEinfo_label="pHAGE 3' CDS 1-1 (Binds IRES)"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    570..577
                     /locus_tag="G-T cluster"
                     /label="G-T cluster"
                     /ApEinfo_label="G-T cluster"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(158..179)
                     /locus_tag="pHAGE-A0050-8050"
                     /label="pHAGE-A0050-8050"
                     /ApEinfo_label="pHAGE-A0050-8050"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6063..6164
                     /locus_tag="HIV nef"
                     /label="HIV nef"
                     /ApEinfo_label="HIV nef"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1650..1678)
                     /locus_tag="pHAGE-A1550-1050"
                     /label="pHAGE-A1550-1050"
                     /ApEinfo_label="pHAGE-A1550-1050"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1156..2013
                     /locus_tag="env"
                     /label="env"
                     /ApEinfo_label="env"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     898..919
                     /locus_tag="pHAGE-S1001-1500"
                     /label="pHAGE-S1001-1500"
                     /ApEinfo_label="pHAGE-S1001-1500"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mutation        2531..2531
                     /locus_tag="EF1a Mutation"
                     /label="EF1a Mutation"
                     /ApEinfo_label="EF1a Mutation"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     stem_loop       793..806
                     /locus_tag="SL-4"
                     /label="SL-4"
                     /ApEinfo_label="SL-4"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5467..6058
                     /locus_tag="WPRE"
                     /label="WPRE"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    853..855
                     /locus_tag="Stop Codon"
                     /label="Stop Codon"
                     /ApEinfo_label="Stop Codon"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     401..421
                     /locus_tag="pHAGE-S501-1000"
                     /label="pHAGE-S501-1000"
                     /ApEinfo_label="pHAGE-S501-1000"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     splicing_signal 744..745
                     /locus_tag="5' SD 289"
                     /label="5' SD 289"
                     /ApEinfo_label="5' SD 289"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     8416..8435
                     /locus_tag="pHAGE-S8001-8500"
                     /label="pHAGE-S8001-8500"
                     /ApEinfo_label="pHAGE-S8001-8500"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_structure  4165..4742
                     /locus_tag="IRES"
                     /label="IRES"
                     /ApEinfo_label="IRES"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4719..4742
                     /locus_tag="pHAGE 5' CDS 2-2 (Binds IRES)"
                     /label="pHAGE 5' CDS 2-2 (Binds IRES)"
                     /ApEinfo_label="pHAGE 5' CDS 2-2 (Binds IRES)"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(5573..5596)
                     /locus_tag="pHAGE-A3550-3050"
                     /label="pHAGE-A3550-3050"
                     /ApEinfo_label="pHAGE-A3550-3050"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          2454..3045
                     /locus_tag="Intron"
                     /label="Intron"
                     /ApEinfo_label="Intron"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4181..4201
                     /locus_tag="pHAGE 5' IRES"
                     /label="pHAGE 5' IRES"
                     /ApEinfo_label="pHAGE 5' IRES"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     6416..6438
                     /locus_tag="pHAGE-S6001-6500"
                     /label="pHAGE-S6001-6500"
                     /ApEinfo_label="pHAGE-S6001-6500"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1391..1417
                     /locus_tag="pHAGE-S1501-2000"
                     /label="pHAGE-S1501-2000"
                     /ApEinfo_label="pHAGE-S1501-2000"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     5726..5746
                     /locus_tag="pHAGE-S4001-4500"
                     /label="pHAGE-S4001-4500"
                     /ApEinfo_label="pHAGE-S4001-4500"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1..454
                     /locus_tag="HIV U3"
                     /label="HIV U3"
                     /ApEinfo_label="HIV U3"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     7913..7937
                     /locus_tag="pHAGE-S7501-8000"
                     /label="pHAGE-S7501-8000"
                     /ApEinfo_label="pHAGE-S7501-8000"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3394..3411
                     /locus_tag="PB2S009_F_EF1a"
                     /label="PB2S009_F_EF1a"
                     /ApEinfo_label="PB2S009_F_EF1a"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6587..6621
                     /locus_tag="P-Bla"
                     /label="P-Bla"
                     /ApEinfo_label="P-Bla"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6302..6384
                     /locus_tag="HIV U5"
                     /label="HIV U5"
                     /ApEinfo_label="HIV U5"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    552..636
                     /locus_tag="HIV U5(1)"
                     /label="HIV U5(1)"
                     /ApEinfo_label="HIV U5"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(6689..6712)
                     /locus_tag="pHAGE-A6050-5550"
                     /label="pHAGE-A6050-5550"
                     /ApEinfo_label="pHAGE-A6050-5550"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(7669..7690)
                     /locus_tag="pHAGE-A7050-6550"
                     /label="pHAGE-A7050-6550"
                     /ApEinfo_label="pHAGE-A7050-6550"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     stem_loop       697..731
                     /locus_tag="SL-1"
                     /label="SL-1"
                     /ApEinfo_label="SL-1"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(8656..8682)
                     /locus_tag="pHAGE-A8050-7550"
                     /label="pHAGE-A8050-7550"
                     /ApEinfo_label="pHAGE-A8050-7550"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6113..6127
                     /locus_tag="HIV PPT"
                     /label="HIV PPT"
                     /ApEinfo_label="HIV PPT"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             4743..5453
                     /locus_tag="mCherry"
                     /label="mCherry"
                     /ApEinfo_label="mCherry"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_site      550..550
                     /locus_tag="pA Site"
                     /label="pA Site"
                     /ApEinfo_label="pA Site"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(7158..7181)
                     /locus_tag="pHAGE-A6550-6050"
                     /label="pHAGE-A6550-6050"
                     /ApEinfo_label="pHAGE-A6550-6050"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2034..2211
                     /locus_tag="HIV cpPu (Trip)"
                     /label="HIV cpPu (Trip)"
                     /ApEinfo_label="HIV cpPu (Trip)"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2084..2112)
                     /locus_tag="pHAGE-A2050-1550"
                     /label="pHAGE-A2050-1550"
                     /ApEinfo_label="pHAGE-A2050-1550"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(5495..5529)
                     /locus_tag="pHAGE 3' CDS"
                     /label="pHAGE 3' CDS"
                     /ApEinfo_label="pHAGE 3' CDS"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2218..3402
                     /locus_tag="Human EF1a Promoter"
                     /label="Human EF1a Promoter"
                     /ApEinfo_label="Human EF1a Promoter"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             6640..7479
                     /locus_tag="AMPr"
                     /label="AMPr"
                     /ApEinfo_label="AMPr"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    636..658
                     /locus_tag="PBS-K"
                     /label="PBS-K"
                     /ApEinfo_label="PBS-K"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     LTR             6063..6384
                     /locus_tag="3' LTR"
                     /label="3' LTR"
                     /ApEinfo_label="3' LTR"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4445..4463
                     /locus_tag="pHAGE 5' CDS 2-1 Start (Binds IRES)"
                     /label="pHAGE 5' CDS 2-1 Start (Binds IRES)"
                     /ApEinfo_label="pHAGE 5' CDS 2-1 Start (Binds IRES)"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6205..6301
                     /locus_tag="HIV R"
                     /label="HIV R"
                     /ApEinfo_label="HIV R"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    637..1155
                     /locus_tag="PSI"
                     /label="PSI"
                     /ApEinfo_label="PSI"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     7409..7433
                     /locus_tag="pHAGE-S7001-7500"
                     /label="pHAGE-S7001-7500"
                     /ApEinfo_label="pHAGE-S7001-7500"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    807..1144
                     /locus_tag="HR-ePSI"
                     /label="HR-ePSI"
                     /ApEinfo_label="HR-ePSI"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3412..4158
                     /locus_tag="Tet-On(R) 3G"
                     /label="Tet-On(R) 3G"
                     /ApEinfo_label="Tet-On(R) 3G"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1166..1189)
                     /locus_tag="pHAGE-A1050-0550"
                     /label="pHAGE-A1050-0550"
                     /ApEinfo_label="pHAGE-A1050-0550"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(4159..4174)
                     /locus_tag="PB2S009_R_IRES"
                     /label="PB2S009_R_IRES"
                     /ApEinfo_label="PB2S009_R_IRES"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(4721..4742)
                     /locus_tag="pHAGE 3' IRES"
                     /label="pHAGE 3' IRES"
                     /ApEinfo_label="pHAGE 3' IRES"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3411..3411
                     /locus_tag="C_for_Kozak"
                     /label="C_for_Kozak"
                     /ApEinfo_label="C_for_Kozak"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1898..1922
                     /locus_tag="pHAGE-S2001-2500"
                     /label="pHAGE-S2001-2500"
                     /ApEinfo_label="pHAGE-S2001-2500"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8478..8601
                     /locus_tag="SV40 ori"
                     /label="SV40 ori"
                     /ApEinfo_label="SV40 ori"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3352..3374
                     /locus_tag="seq_EF1a_3352F"
                     /label="seq_EF1a_3352F"
                     /ApEinfo_label="seq_EF1a_3352F"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(4445..4463)
                     /locus_tag="pHAGE 3' CDS 1-2 (Binds IRES)"
                     /label="pHAGE 3' CDS 1-2 (Binds IRES)"
                     /ApEinfo_label="pHAGE 3' CDS 1-2 (Binds IRES)"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6130..6166
                     /locus_tag="dU3"
                     /label="dU3"
                     /ApEinfo_label="dU3"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    455..551
                     /locus_tag="HIV R(1)"
                     /label="HIV R(1)"
                     /ApEinfo_label="HIV R"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     stem_loop       736..754
                     /locus_tag="SL-2"
                     /label="SL-2"
                     /ApEinfo_label="SL-2"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     6889..6913
                     /locus_tag="pHAGE-S6501-7000"
                     /label="pHAGE-S6501-7000"
                     /ApEinfo_label="pHAGE-S6501-7000"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    697..806
                     /locus_tag="HIV-PSI"
                     /label="HIV-PSI"
                     /ApEinfo_label="HIV-PSI"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6186..6204
                     /locus_tag="dU3(1)"
                     /label="dU3(1)"
                     /ApEinfo_label="dU3"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mutation        833..834
                     /locus_tag="Fill-in"
                     /label="Fill-in"
                     /ApEinfo_label="Fill-in"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc catgtctaga
     3421 ctggacaaga gcaaagtcat aaactctgct ctggaattac tcaatggagt cggtatcgaa
     3481 ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg
     3541 cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat
     3601 catacccact cctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc
     3661 aagtcatacc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc
     3721 cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa
     3781 ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc
     3841 tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc
     3901 gattctatgc ccccacttct gaaacaagca attgagctgt tcgaccggca gggagccgaa
     3961 cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc
     4021 gaaagcggcg ggccgaccga cgcccttgac gattttgact tagacatgct cccagccgat
     4081 gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt
     4141 gacatgctcc ccgggtaagg atccctcccc cccccctaac gttactggcc gaagccgctt
     4201 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     4261 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     4321 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     4381 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     4441 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     4501 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     4561 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     4621 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     4681 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacac
     4741 atatggtcag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     4801 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     4861 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     4921 ccttcgcctg ggacatcctg tcccctcaat tcatgtatgg ctccaaggcc tacgtgaagc
     4981 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     5041 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccttacaag
     5101 acggcgagtt catctacaaa gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     5161 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     5221 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     5281 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     5341 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     5401 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag taaaatcgat
     5461 agatcctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     5521 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     5581 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     5641 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     5701 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     5761 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     5821 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     5881 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     5941 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6001 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     6061 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6121 aggggggact ggaagggcta attcactccc aacgaagaca agatcacctg caggacaggc
     6181 gcgccctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6241 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6301 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6361 agtcagtgtg gaaaatctct agcacccggg cgattaagga aagggctaga tcattcttga
     6421 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     6481 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6541 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6601 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6661 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6721 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6781 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6841 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     6901 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6961 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     7021 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     7081 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7141 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     7201 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     7261 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     7321 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     7381 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     7441 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     7501 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7561 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7621 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7681 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7741 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7801 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7861 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7921 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7981 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     8041 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     8101 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8161 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     8221 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     8281 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     8341 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     8401 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     8461 ccgattcatt aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg
     8521 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     8581 gcttttgcaa aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag
     8641 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     8701 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     8761 tatgacatga ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8821 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
     8881 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
     8941 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
     9001 taaatatgta ctacaaactt agtagt   
//
