LOCUS       pLenti-tetON-NOT       10178 bp DNA     circular SYN 10-JUN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2023 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10178
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     primer_bind     complement(859..878)
                     /label=SP_HIV_Rev
                     /note="color: black; sequence: ATATTTTTTCTTTCCCCCTG"
     primer_bind     1220..1241
                     /label=Up_RRE_For
                     /note="color: black; sequence: gtagtaaaaattgaaccattag"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        2242..2616
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
                     /note="color: #99ccff"
     protein_bind    2246..2264
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2282..2300
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2318..2336
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2354..2372
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2390..2408
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2426..2444
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2462..2480
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     misc_feature    2493..2557
                     /label=Common to CMV
                     /note="color: #ff99cc"
     misc_feature    2617..2629
                     /label=FRT Repeat
                     /note="color: #00ffff"
     misc_feature    2631..2643
                     /label=FRT Repeat
                     /note="color: #00ffff"
     misc_feature    2644..2651
                     /label=FRT5 Spacer
                     /note="color: #3366ff"
     misc_feature    2652..2664
                     /label=FRT Inv Repeat
                     /note="color: #00ffff"
     misc_feature    2680..4170
                     /label=NOTUM CDS
                     /note="color: #993300"
     polyA_site      4248..4303
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        4473..5018
                     /locus_tag="EF1\promoter"
                     /label=EF1a promoter
                     /note="color: #ffcc99"
     CDS             5038..5748
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             5755..5808
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 5805"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5809..6555
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /note="color: #993366"
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6568..7156
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(7039..7050)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 7038"
                     /translation="IEGR"
     LTR             7231..7464
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        7669..7773
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             7774..8634
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7774 .. 7842 / #ccffcc / signal sequence
                        2: 7843 .. 8634 / #ccffcc
                     Cleavage site after base 7842"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7992..8011)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     rep_origin      8805..9393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     9294..9313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     primer_bind     9547..9564
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     protein_bind    9804..9825
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        9840..9870
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 9840 .. 9845 / #ffffff / -35
                        2: 9846 .. 9863 / #ffffff
                        3: 9864 .. 9870 / #ffffff / -10"
     protein_bind    9878..9894
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     9883..9905
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     primer_bind     9902..9918
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     9902..9918
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     9968..9987
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagaccg
     2221 gtggcctcca cggccactag attactccct atcagtgata gagaacgtat gaagagttta
     2281 ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt
     2341 ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt
     2401 gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat atccagttta
     2461 ctccctatca gtgatagaga acgtataagc tttaggcgtg tacggtgggc gcctataaaa
     2521 gcagagctcg tttagtgaac cgtcagatcg cctggagcaa ttccacaaca cttttgtctt
     2581 ataccaactt tccgtaccac ttcctaccct cgtaaagaag ttcctattcc gaagttccta
     2641 ttcttcaaaa ggtataggaa cttcgctcga gccgccacca tgggccgagg ggtgcgcgtg
     2701 ctgctgctgc tgagcctgct gcactgcgcc gggggcagcg agggcaggaa gacctggcgg
     2761 cgccggggtc agcagccgcc tcctcccccg cggaccgagg cggcgccggc ggccggacag
     2821 cccgtggaga gcttcccgct ggacttcacg gccgtggagg gtaacatgga cagcttcatg
     2881 gcgcaagtca agagcctggc gcagtccctg tacccctgct ccgcgcagca gctcaacgag
     2941 gacctgcgcc tgcacctcct actcaacacc tcggtgacct gcaacgacgg cagccccgcc
     3001 ggctactacc tgaaggagtc caggggcagc cggcggtggc tcctcttcct ggaaggcggc
     3061 tggtactgct tcaaccgcga gaactgcgac tccagatacg acaccatgcg gcgcctcatg
     3121 agctcccggg actggccgcg cactcgcaca ggcacaggga tcctgtcctc acagccggag
     3181 gagaacccct actggtggaa cgcaaacatg gtcttcatcc cctactgctc cagtgatgtt
     3241 tggagcgggg cttcatccaa gtctgagaag aacgagtacg ccttcatggg cgccctcatc
     3301 atccaggagg tggtgcggga gcttctgggc agagggctga gcggggccaa ggtgctgctg
     3361 ctggccggga gcagcgcggg gggcaccggg gtgctcctga atgtggaccg tgtggctgag
     3421 cagctggaga agctgggcta cccagccatc caggtgcgag gcctggctga ctccggctgg
     3481 ttcctggaca acaagcagta tcgccacaca gactgcgtcg acacgatcac gtgcgcgccc
     3541 acggaggcca tccgccgtgg catcaggtac tggaacgggg tggtcccgga gcgctgccga
     3601 cgccagttcc aggagggcga ggagtggaac tgcttctttg gctacaaggt ctacccgacc
     3661 ctgcgctgcc ctgtgttcgt ggtgcagtgg ctgtttgacg aggcacagct gacggtggac
     3721 aacgtgcacc tgacggggca gccggtgcag gagggcctgc ggctgtacat ccagaacctc
     3781 ggccgcgagc tgcgccacac actcaaggac gtgccggcca gctttgcccc cgcctgcctc
     3841 tcccatgaga tcatcatccg gagccactgg acggatgtcc aggtgaaggg gacgtcgctg
     3901 ccccgagcac tgcactgctg ggacaggagc ctccatgaca gccacaaggc cagcaagacc
     3961 cccctcaagg gctgccccgt ccacctggtg gacagctgcc cctggcccca ctgcaacccc
     4021 tcatgcccca ccgtccgaga ccagttcacg gggcaagaga tgaacgtggc ccagttcctc
     4081 atgcacatgg gcttcgacat gcagacggtg gcccagccgc agggactgga gcccagtgag
     4141 ctgctgggga tgctgagcaa cggaagctag gcggccgcct aatctttttc cctctgccaa
     4201 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     4261 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     4321 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     4381 catatgctgg ctgccatgaa caaaggtggc tataaagagg tcatcagtat atgaaacagc
     4441 cccctgctgt ccattcctta catatgacta gtaaggatct gcgatcgctc cggtgcccgt
     4501 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     4561 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     4621 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     4681 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     4741 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     4801 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     4861 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     4921 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     4981 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacac ctgcagccaa cgccaccatg
     5041 gtgtctaagg gcgaagagct gattaaggag aacatgcaca tgaagctgta catggagggc
     5101 accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
     5161 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
     5221 ctggctacca gcttcatgta cggcagcaga accttcatca accacaccca gggcatcccc
     5281 gacttcttta agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
     5341 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
     5401 aacgtcaaga tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca
     5461 ctcggctggg aggccaacac cgagatgctg taccccgctg acggcggcct ggaaggcaga
     5521 agcgacatgg ccctgaagct cgtgggcggg ggccacctga tctgcaactt caagaccaca
     5581 tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctacta tgtggaccac
     5641 agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgaggtggct
     5701 gtggccagat actgcgacct ccctagcaaa ctggggcaca aacttaatgc tagcgagggc
     5761 agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccaat gtctagactg
     5821 gacaagagca aagtcataaa ctctgctctg gaattactca atggagtcgg tatcgaaggc
     5881 ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac
     5941 gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat
     6001 acccactcct gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag
     6061 tcataccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc
     6121 ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc
     6181 ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc
     6241 gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat
     6301 tctatgcccc cacttctgaa acaagcaatt gagctgttcg accggcaggg agccgaacct
     6361 gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa
     6421 agcggcgggc cgaccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc
     6481 cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac
     6541 atgctccccg ggtaagtcga cgatcctaat caacctctgg attacaaaat ttgtgaaaga
     6601 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6661 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6721 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6781 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     6841 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6901 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6961 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7021 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7081 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7141 tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc
     7201 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     7261 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7321 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7381 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7441 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     7501 aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta
     7561 gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     7621 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     7681 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     7741 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7801 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7861 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7921 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7981 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     8041 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     8101 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     8161 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8221 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     8281 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     8341 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     8401 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     8461 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     8521 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     8581 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     8641 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8701 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8761 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8821 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8881 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8941 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9001 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9061 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9121 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9181 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9241 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9301 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     9361 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     9421 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     9481 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     9541 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     9601 cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt
     9661 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     9721 ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa
     9781 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     9841 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     9901 acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat
     9961 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    10021 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    10081 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    10141 aattgtattt gttaaatatg tactacaaac ttagtagt
//
