LOCUS       pLenti-tetON-GAT       10464 bp DNA     circular SYN 10-JUN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2023 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10464
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     primer_bind     complement(859..878)
                     /label=SP_HIV_Rev
                     /note="color: black; sequence: ATATTTTTTCTTTCCCCCTG"
     primer_bind     1220..1241
                     /label=Up_RRE_For
                     /note="color: black; sequence: gtagtaaaaattgaaccattag"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        2242..2616
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
                     /note="color: #99ccff"
     protein_bind    2246..2264
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2282..2300
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2318..2336
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2354..2372
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2390..2408
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2426..2444
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2462..2480
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     misc_feature    2493..2557
                     /label=Common to CMV
                     /note="color: #ff99cc"
     misc_feature    2617..2629
                     /label=FRT Repeat
                     /note="color: #00ffff"
     misc_feature    2631..2643
                     /label=FRT Repeat
                     /note="color: #00ffff"
     misc_feature    2644..2651
                     /label=FRT5 Spacer
                     /note="color: #3366ff"
     misc_feature    2652..2664
                     /label=FRT Inv Repeat
                     /note="color: #00ffff"
     misc_feature    2671..4458
                     /label=GATA6 CDS
                     /note="color: #00ffff"
     polyA_site      4534..4589
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        4759..5304
                     /locus_tag="EF1\promoter"
                     /label=EF1a promoter
                     /note="color: #ffcc99"
     CDS             5324..6034
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             6041..6094
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 6091"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6095..6841
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /note="color: #993366"
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6854..7442
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(7325..7336)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 7324"
                     /translation="IEGR"
     LTR             7517..7750
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     complement(7869..7887)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /note="color: black; sequence: AATAGGCGTATCACGAGGC"
     promoter        7955..8059
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             8060..8920
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8060 .. 8128 / #ccffcc / signal sequence
                        2: 8129 .. 8920 / #ccffcc
                     Cleavage site after base 8128"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8278..8297)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     rep_origin      9091..9679
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     9580..9599
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     primer_bind     9833..9850
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     primer_bind     9942..9961
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     primer_bind     complement(9965..9976)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     protein_bind    10090..10111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        10126..10156
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 10126 .. 10131 / #ffffff / -35
                        2: 10132 .. 10149 / #ffffff
                        3: 10150 .. 10156 / #ffffff / -10"
     protein_bind    10164..10180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     10169..10191
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     primer_bind     10188..10204
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     10188..10204
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     10254..10273
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagaccg
     2221 gtggcctcca cggccactag attactccct atcagtgata gagaacgtat gaagagttta
     2281 ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt
     2341 ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt
     2401 gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat atccagttta
     2461 ctccctatca gtgatagaga acgtataagc tttaggcgtg tacggtgggc gcctataaaa
     2521 gcagagctcg tttagtgaac cgtcagatcg cctggagcaa ttccacaaca cttttgtctt
     2581 ataccaactt tccgtaccac ttcctaccct cgtaaagaag ttcctattcc gaagttccta
     2641 ttcttcaaaa ggtataggaa cttcgctcga atggccttga ctgacggcgg ctggtgcttg
     2701 ccgaagcgct tcggggccgc gggtgcggac gccagcgact ccagagcctt tccagcgcgg
     2761 gagccctcca cgccgccttc ccccatctct tcctcgtcct cctcctgctc ccggggcgga
     2821 gagcggggcc ccggcggcgc cagcaactgc gggacgcctc agctcgacac ggaggcggcg
     2881 gccggacccc cggcccgctc gctgctgctc agttcctacg cttcgcatcc cttcggggct
     2941 ccccacggac cttcggcgcc tggggtcgcg ggccccgggg gcaacctgtc gagctgggag
     3001 gacttgctgc tgttcactga cctcgaccaa gccgcgaccg ccagcaagct gctgtggtcc
     3061 agccgcggcg ccaagctgag ccccttcgca cccgagcagc cggaggagat gtaccagacc
     3121 ctcgccgctc tctccagcca gggtccggcc gcctacgacg gcgcgcccgg cggcttcgtg
     3181 cactctgcgg ccgcggcggc agcagccgcg gcggcggcca gctccccggt ctacgtgccc
     3241 accacccgcg tgggttccat gctgcccggc ctaccgtacc acctgcaggg gtcgggcagt
     3301 gggccagcca accacgcggg cggcgcgggc gcgcaccccg gctggcctca ggcctcggcc
     3361 gacagccctc catacggcag cggaggcggc gcggctggcg gcggggccgc ggggcctggc
     3421 ggcgctggct cagccgcggc gcacgtctcg gcgcgcttcc cctactctcc cagcccgccc
     3481 atggccaacg gcgccgcgcg ggagccggga ggctacgcgg cggcgggcag tgggggcgcg
     3541 ggaggcgtga gcggcggcgg cagtagcctg gcggccatgg gcggccgcga gccccagtac
     3601 agctcgctgt cggccgcgcg gccgctgaac gggacgtacc accaccacca ccaccaccac
     3661 caccaccatc cgagccccta ctcgccctac gtgggggcgc cactgacgcc tgcctggccc
     3721 gccggaccct tcgagacccc ggtgctgcac agcctgcaga gccgcgccgg agccccgctc
     3781 ccggtgcccc ggggtcccag tgcagacctg ctggaggacc tgtccgagag ccgcgagtgc
     3841 gtgaactgcg gctccatcca gacgccgctg tggcggcggg acggcaccgg ccactacctg
     3901 tgcaacgcct gcgggctcta cagcaagatg aacggcctca gccggcccct catcaagccg
     3961 cagaagcgcg tgccttcatc acggcggctt ggattgtcct gtgccaactg tcacaccaca
     4021 actaccacct tatggcgcag aaacgccgag ggtgaacccg tgtgcaatgc ttgtggactc
     4081 tacatgaaac tccatggggt gcccagacca cttgctatga aaaaagaggg aattcaaacc
     4141 aggaaacgaa aacctaagaa cataaataaa tcaaagactt gctctggtaa tagcaataat
     4201 tccattccca tgactccaac ttccacctct tctaactcag atgattgcag caaaaatact
     4261 tcccccacaa cacaacctac agcctcaggg gcgggtgccc cggtgatgac tggtgcggga
     4321 gagagcacca atcccgagaa cagcgagctc aagtattcgg gtcaagatgg gctctacata
     4381 ggcgtcagtc tcgcctcgcc ggccgaagtc acgtcctccg tgcgaccgga ttcctggtgc
     4441 gccctggccc tggcctgagg ccgcctaatc tttttccctc tgccaaaaat tatggggaca
     4501 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
     4561 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     4621 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgccata tgctggctgc
     4681 catgaacaaa ggtggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     4741 tccttacata tgactagtaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg
     4801 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta
     4861 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     4921 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     4981 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc
     5041 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc
     5101 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc
     5161 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg
     5221 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag
     5281 atccaagctg tgaccggcgc ctacacctgc agccaacgcc accatggtgt ctaagggcga
     5341 agagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca
     5401 ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
     5461 aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt
     5521 catgtacggc agcagaacct tcatcaacca cacccagggc atccccgact tctttaagca
     5581 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
     5641 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag
     5701 aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
     5761 caacaccgag atgctgtacc ccgctgacgg cggcctggaa ggcagaagcg acatggccct
     5821 gaagctcgtg ggcgggggcc acctgatctg caacttcaag accacataca gatccaagaa
     5881 acccgctaag aacctcaaga tgcccggcgt ctactatgtg gaccacagac tggaaagaat
     5941 caaggaggcc gacaaagaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg
     6001 cgacctccct agcaaactgg ggcacaaact taatgctagc gagggcagag gaagtctgct
     6061 aacatgcggt gacgtcgagg agaatcctgg cccaatgtct agactggaca agagcaaagt
     6121 cataaactct gctctggaat tactcaatgg agtcggtatc gaaggcctga cgacaaggaa
     6181 actcgctcaa aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg
     6241 ggccctgctc gatgccctgc caatcgagat gctggacagg catcataccc actcctgccc
     6301 cctggaaggc gagtcatggc aagactttct gcggaacaac gccaagtcat accgctgtgc
     6361 tctcctctca catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca
     6421 gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa
     6481 cgcactgtac gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca
     6541 ggagcatcaa gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact
     6601 tctgaaacaa gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg
     6661 cctggaacta atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccgac
     6721 cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga
     6781 ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta
     6841 agtcgacgat cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6901 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6961 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     7021 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     7081 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7141 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7201 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     7261 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7321 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7381 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7441 gcctgagatc ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7501 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     7561 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7621 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7681 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7741 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     7801 tgaatatcag agagtgagag gcccgggtta attaaggaaa gggctagatc attcttgaag
     7861 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     7921 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     7981 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     8041 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     8101 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8161 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     8221 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     8281 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     8341 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8401 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8461 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8521 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8581 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8641 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8701 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8761 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8821 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8881 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     8941 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9001 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9061 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9121 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9181 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     9241 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9301 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9361 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9421 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9481 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9541 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9601 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9661 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9721 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9781 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9841 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     9901 gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag
     9961 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
    10021 ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
    10081 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
    10141 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
    10201 tgacatgatt acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    10261 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    10321 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    10381 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    10441 aatatgtact acaaacttag tagt
//
