LOCUS       Exported               13582 bp ds-DNA     linear   SYN 09-JUN-2023
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13582)
  AUTHORS   wallv
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 11, 2020 from SnapGene 4.3.11
            https://www.snapgene.com
REFERENCE   2  (bases 1 to 13582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 9, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13582
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      204..1993
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
                     /note="color: #ffff00"
     protein_bind    2199..2219
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP  
                     reaction"
                     /note="""color: #31849b"
                     /note="direction: RIGHT"""
     enhancer        2230..2533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2534..2737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early  
                     promoter"
                     /note="""color: #ffffff"
                     /note="direction: RIGHT"""
     protein_bind    2805..2825
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP  
                     reaction"
                     /note="""color: #31849b"
                     /note="direction: LEFT"""
     LTR             2829..3097
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human  
                     T-cell leukemia virus (HTLV) type 1"
                     /note="color: #ffe4c4"
     CDS             3104..9046
                     /codon_start=1
                     /label=3xFLAG-SARS-CoV-2 Nsp3
                     /note="color: #0000ff"
                     /translation="MGDYKDHDGDYKDHDIDYKDDDDKGSTTLYKKVGMAPTKVTFGDD
                     TVIEVQGYKSVNITFELDERIDKVLNEKCSAYTVELGTEVNEFACVVADAVIKTLQPVS
                     ELLTPLGIDLDEWSMATYYLFDESGEFKLASHMYCSFYPPDEDEEEGDCEEEEFEPSTQ
                     YEYGTEDDYQGKPLEFGATSAALQPEEEQEEDWLDDDSQQTVGQQDGSEDNQTTTIQTI
                     VEVQPQLEMELTPVVQTIEVNSFSGYLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVY
                     LKHGGGVAGALNKATNNAMQVESDDYIATNGPLKVGGSCVLSGHNLAKHCLHVVGPNVN
                     KGEDIQLLKSAYENFNQHEVLLAPLLSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNL
                     YDKLVSSFLEMKSEKQVEQKIAEIPKEEVKPFITESKPSVEQRKQDDKKIKACVEEVTT
                     TLEETKFLTENLLLYIDINGNLHPDSATLVSDIDITFLKKDAPYIVGDVVQEGVLTAVV
                     IPTKKAGGTTEMLAKALRKVPTDNYITTYPGQGLNGYTVEEAKTVLKKCKSAFYILPSI
                     ISNEKQEILGTVSWNLREMLAHAEETRKLMPVCVETKAIVSTIQRKYKGIKIQEGVVDY
                     GARFYFYTSKTTVASLINTLNDLNETLVTMPLGYVTHGLNLEEAARYMRSLKVPATVSV
                     SSPDAVTAYNGYLTSSSKTPEEHFIETISLAGSYKDWSYSGQSTQLGIEFLKRGDKSVY
                     YTSNPTTFHLDGEVITFDNLKTLLSLREVRTIKVFTTVDNINLHTQVVDMSMTYGQQFG
                     PTYLDGADVTKIKPHNSHEGKTFYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTK
                     KWKYPQVNGLTSIKWADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCAL
                     ILAYCNKTVGELGDVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMG
                     TLSYEQFKKGVQIPCTCGKQATKYLVQQESPFVMMSAPPAQYELKHGTFTCASEYTGNY
                     QCGHYKHITSKETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCT
                     EIDPKLDNYYKKDNSYFTEQPIDLVPNQPYPNASFDNFKFVCDNIKFADDLNQLTGYKK
                     PASRELKVTFFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTW
                     CIRCLWSTKPVETSNSFDVLKSEDAQGMDNLACEDLKPVSEEVVENPTIQKDVLECNVK
                     TTEVVGDIILKPANNSLKITEEVGHTDLMAAYVDNSSLTIKKPNELSRVLGLKTLATHG
                     LAAVNSVPWDTIANYAKPFLNKVVSTTTNIVTRCLNRVCTNYMPYFFTLLLQLCTFTRS
                     TNSRIKASMPTTIAKNTVKSVGKFCLEASFNYLKSPNFSKLINIIIWFLLLSVCLGSLI
                     YSTAALGVLMSNLGMPSYCTGYREGYLNSTNVTIATYCTGSIPCSVCLSGLDSLDTYPS
                     LETIQITISSFKWDLTAFGLVAEWFLAYILFTRFFYVLGLAAIMQLFFSYFAVHFISNS
                     WLMWLIINLVQMAPISAMVRMYIFFASFYYVWKSYVHVVDGCNSSTCMMCYKRNRATRV
                     ECTTIVNGVRRSFYVYANGGKGFCKLHNWNCVNCDTFCAGSTFISDEVARDLSLQFKRP
                     INPTDQSSYIVDSVTVKNGSIHLYFDKAGQKTYERHSLSHFVNLDNLRANNTKGSLPIN
                     VIVFDGKSKCEESSAKSASVYYSQLMCQPILLLDQALVSDVGDSAEVAVKMFDAYVNTF
                     SSTFNVPMEKLKTLVATAEAELAKNVSLDNVLSTFISAARQGFVDSDVETKDVVECLKL
                     SHQSDIEVTGDSCNNYMLTYNKVENMTPRDLGACIDCSARHINAQVAKSHNIALIWNVK
                     DFMSLSEQLRKQIRSAAKKNNLPFKLTCATTRQVVNVVTTKIALKGG"
     CDS             3110..3175
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2 Cleavage site after base 3175"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    3182..3206
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="""color: #31849b"
                     /note="direction: RIGHT"""
     CDS             3206..9046
                     /codon_start=1
                     /label=SARS-CoV-2 Nsp3
                     /note="color: #993366"
                     /translation="MAPTKVTFGDDTVIEVQGYKSVNITFELDERIDKVLNEKCSAYTV
                     ELGTEVNEFACVVADAVIKTLQPVSELLTPLGIDLDEWSMATYYLFDESGEFKLASHMY
                     CSFYPPDEDEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLEFGATSAALQPEEEQEEDWL
                     DDDSQQTVGQQDGSEDNQTTTIQTIVEVQPQLEMELTPVVQTIEVNSFSGYLKLTDNVY
                     IKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATNNAMQVESDDYIATNGPLK
                     VGGSCVLSGHNLAKHCLHVVGPNVNKGEDIQLLKSAYENFNQHEVLLAPLLSAGIFGAD
                     PIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSEKQVEQKIAEIPKEEVKPFIT
                     ESKPSVEQRKQDDKKIKACVEEVTTTLEETKFLTENLLLYIDINGNLHPDSATLVSDID
                     ITFLKKDAPYIVGDVVQEGVLTAVVIPTKKAGGTTEMLAKALRKVPTDNYITTYPGQGL
                     NGYTVEEAKTVLKKCKSAFYILPSIISNEKQEILGTVSWNLREMLAHAEETRKLMPVCV
                     ETKAIVSTIQRKYKGIKIQEGVVDYGARFYFYTSKTTVASLINTLNDLNETLVTMPLGY
                     VTHGLNLEEAARYMRSLKVPATVSVSSPDAVTAYNGYLTSSSKTPEEHFIETISLAGSY
                     KDWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVITFDNLKTLLSLREVRTIKV
                     FTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPHNSHEGKTFYVLPNDDTLRV
                     EAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIKWADNNCYLATALLTLQQIEL
                     KFNPPALQDAYYRARAGEAANFCALILAYCNKTVGELGDVRETMSYLFQHANLDSCKRV
                     LNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQIPCTCGKQATKYLVQQESPFVM
                     MSAPPAQYELKHGTFTCASEYTGNYQCGHYKHITSKETLYCIDGALLTKSSEYKGPITD
                     VFYKENSYTTTIKPVTYKLDGVVCTEIDPKLDNYYKKDNSYFTEQPIDLVPNQPYPNAS
                     FDNFKFVCDNIKFADDLNQLTGYKKPASRELKVTFFPDLNGDVVAIDYKHYTPSFKKGA
                     KLLHKPIVWHVNNATNKATYKPNTWCIRCLWSTKPVETSNSFDVLKSEDAQGMDNLACE
                     DLKPVSEEVVENPTIQKDVLECNVKTTEVVGDIILKPANNSLKITEEVGHTDLMAAYVD
                     NSSLTIKKPNELSRVLGLKTLATHGLAAVNSVPWDTIANYAKPFLNKVVSTTTNIVTRC
                     LNRVCTNYMPYFFTLLLQLCTFTRSTNSRIKASMPTTIAKNTVKSVGKFCLEASFNYLK
                     SPNFSKLINIIIWFLLLSVCLGSLIYSTAALGVLMSNLGMPSYCTGYREGYLNSTNVTI
                     ATYCTGSIPCSVCLSGLDSLDTYPSLETIQITISSFKWDLTAFGLVAEWFLAYILFTRF
                     FYVLGLAAIMQLFFSYFAVHFISNSWLMWLIINLVQMAPISAMVRMYIFFASFYYVWKS
                     YVHVVDGCNSSTCMMCYKRNRATRVECTTIVNGVRRSFYVYANGGKGFCKLHNWNCVNC
                     DTFCAGSTFISDEVARDLSLQFKRPINPTDQSSYIVDSVTVKNGSIHLYFDKAGQKTYE
                     RHSLSHFVNLDNLRANNTKGSLPINVIVFDGKSKCEESSAKSASVYYSQLMCQPILLLD
                     QALVSDVGDSAEVAVKMFDAYVNTFSSTFNVPMEKLKTLVATAEAELAKNVSLDNVLST
                     FISAARQGFVDSDVETKDVVECLKLSHQSDIEVTGDSCNNYMLTYNKVENMTPRDLGAC
                     IDCSARHINAQVAKSHNIALIWNVKDFMSLSEQLRKQIRSAAKKNNLPFKLTCATTRQV
                     VNVVTTKIALKGG"
     protein_bind    9048..9072
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="""color: #31849b"
                     /note="direction: LEFT"""
     promoter        complement(9134..9152)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="""color: #ffffff"
                     /note="direction: LEFT"""
     misc_feature    9135..9152
                     /label=SP6
                     /note="""color: #cc0000"
                     /note="direction: LEFT"""
     polyA_signal    9178..9402
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      9448..9876
                     /label=f1 ori
                     /note="""f1 bacteriophage origin of replication"
                     /note="arrow  indicates direction of (+) strand 
                     synthesis"""
                     /note="color: #ffff00"
     promoter        9890..10220
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="""color: #ffffff"
                     /note="direction: RIGHT"""
     rep_origin      10071..10206
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             10287..11081
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    11136..11508
                     /label=pA
                     /note="SV40 polyA"
                     /note="""color: #cc0000"
                     /note="direction: RIGHT"""
     polyA_signal    11255..11376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(11425..11441)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar  
                     variants"
                     /note="""color: #a020f0"
                     /note="direction: LEFT"""
     protein_bind    11449..11465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to  
                     inhibit transcription in E. coli. This inhibition can be  
                     relieved by adding lactose or  
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(11473..11503)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments: 1: 
                     11473 .. 11479 / #ffffff / -10 2: 11480 .. 11497 / #ffffff 
                     3: 11498 .. 11503 / #ffffff / -35"
     protein_bind    11518..11539
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      11827..12415
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
                     /note="color: #ffff00"
     CDS             complement(12586..13446)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments: 1: 12586 .. 13377 / 
                     #ccffcc 2: 13378 .. 13446 / #ccffcc / signal sequence 
                     Cleavage site after base 13377"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13447..13551)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="""color: #ffffff"
                     /note="direction: LEFT"""
ORIGIN
        1 gacggatcgg gagatccgcc tttatgtgta actcttggct gaagctctta caccaatgct
       61 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
      121 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
      181 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
      241 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
      301 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
      361 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
      421 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
      481 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
      541 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
      601 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
      661 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
      721 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
      781 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
      841 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
      901 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
      961 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     1021 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     1081 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     1141 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     1201 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     1261 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     1321 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     1381 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     1441 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     1501 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     1561 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     1621 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1681 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1741 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     1801 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     1861 ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag
     1921 tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct
     1981 tgtccttttc ctgctgggga tctcccgatc ccctatggtg cactctcagt acaatctgct
     2041 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     2101 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     2161 atctgcttag ggttaggcgt tttgcgctgc ttcgatcaca actttgtata gaaaagttgg
     2221 ccctgcaggc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2281 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2341 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2401 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2461 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     2521 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     2581 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2641 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     2701 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2761 ctggagacgc catccacgct gttttgacct ccatagaaga cagccaactt ttgtatacaa
     2821 agtggcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc
     2881 cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg
     2941 ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga
     3001 gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg
     3061 tctttgtttc gttttctgtt ctgcgccgtt acagatcggc accatgggcg actacaaaga
     3121 ccacgacggt gattataagg atcatgacat cgattataag gatgacgatg acaagggcag
     3181 cacaactttg tacaaaaaag ttggcatggc ccctaccaag gtgaccttcg gagacgacac
     3241 cgtgatcgag gtgcagggtt acaagagcgt gaacatcacc ttcgagctgg acgaacgcat
     3301 cgacaaggtg ctgaacgaaa agtgctccgc ttacaccgtg gagctgggca ccgaggtgaa
     3361 cgaattcgcc tgcgtggtgg ccgacgctgt gatcaagacc ctgcagccag tgtccgaact
     3421 gctgacccct ctgggtatcg acctggacga gtggtctatg gctacctact acctgttcga
     3481 cgagagcggc gaattcaagc tggcctctca catgtactgc agcttctacc ctcccgacga
     3541 agacgaggaa gagggtgact gcgaagagga agagttcgag ccttccactc agtacgagta
     3601 cggcaccgaa gacgactacc agggcaagcc tctggaattc ggagccacct ctgccgctct
     3661 gcagcctgaa gaggaacagg aggaagactg gctggacgac gactcccagc agaccgtggg
     3721 tcagcaggac ggctctgaag acaaccagac caccaccatc cagaccatcg tggaggtgca
     3781 gcctcagctg gagatggaac tgaccccagt ggtgcagacc atcgaggtga acagcttctc
     3841 cggctacctg aagctgaccg acaacgtgta catcaagaac gctgacatcg tggaggaagc
     3901 caagaaggtg aaaccaaccg tggtggtgaa cgccgctaac gtgtacctga agcacggcgg
     3961 aggtgtggct ggagctctga acaaggctac caacaacgcc atgcaggtgg aatccgacga
     4021 ctacatcgct accaacggtc ctctgaaggt gggcggatcc tgcgtgctgt ctggccacaa
     4081 cctggccaag cactgcctgc acgtggtggg accaaacgtg aacaagggag aggacatcca
     4141 gctgctgaag agcgcctacg aaaacttcaa ccagcacgag gtgctgctgg ctccactgct
     4201 gtctgccgga atcttcggtg ctgatcccat ccacagcctg cgcgtgtgcg tggacaccgt
     4261 gaggaccaac gtgtacctgg ccgtgttcga caagaacctg tacgacaagc tggtgagctc
     4321 cttcctggag atgaagtccg aaaagcaggt ggagcagaag atcgccgaga tccccaagga
     4381 ggaagtgaag cctttcatca ccgaatctaa gcccagcgtg gagcagagaa agcaggacga
     4441 caagaagatc aaggcctgcg tggaggaagt gaccaccacc ctggaggaaa ccaagttcct
     4501 gaccgagaac ctgctgctgt acatcgacat caacggcaac ctgcaccctg actccgctac
     4561 cctggtgtct gacatcgaca tcaccttcct gaagaaggac gccccctaca tcgtgggcga
     4621 cgtggtgcag gaaggagtgc tgaccgctgt ggtcatccct accaagaagg ccggtggcac
     4681 caccgagatg ctggccaagg ctctgcgtaa ggtgccaacc gacaactaca tcaccaccta
     4741 ccccggtcag ggcctgaacg gttacaccgt ggaggaagct aagaccgtgc tgaagaagtg
     4801 caagtctgcc ttctacatcc tgccctccat catctctaac gagaagcagg aaatcctggg
     4861 caccgtgagc tggaacctgc gcgaaatgct ggcccacgct gaggaaacca ggaagctgat
     4921 gcccgtgtgc gtggagacca aggccatcgt gagcaccatc cagagaaagt acaagggcat
     4981 caagatccag gagggtgtgg tggactacgg cgctcgtttc tacttctaca cctccaagac
     5041 caccgtggcc tctctgatca acaccctgaa cgacctgaac gagaccctgg tgaccatgcc
     5101 actgggatac gtgacccacg gtctgaacct ggaggaagcc gctagataca tgcgttccct
     5161 gaaggtgcct gctaccgtga gcgtgagcag ccctgacgct gtgaccgcct acaacggata
     5221 cctgacctcc tctagcaaga cccctgagga acacttcatc gaaaccatca gcctggccgg
     5281 ttcctacaag gactggtctt acagcggaca gtccacccag ctgggcatcg agttcctgaa
     5341 gcgcggagac aagagcgtgt actacacctc caacccaacc accttccacc tggacggtga
     5401 agtgatcacc ttcgacaacc tgaagaccct gctgtctctg cgcgaggtga ggaccatcaa
     5461 ggtgttcacc accgtggaca acatcaacct gcacacccag gtggtggaca tgagcatgac
     5521 ctacggacag cagttcggtc ctacctacct ggacggagct gacgtgacca agatcaagcc
     5581 acacaactcc cacgagggca agaccttcta cgtgctgcct aacgatgaca ccctgagagt
     5641 ggaggctttc gaatactacc acaccaccga cccatccttc ctgggcaggt acatgtctgc
     5701 cctgaaccac accaagaagt ggaagtaccc ccaggtgaac ggactgacca gcatcaagtg
     5761 ggccgacaac aactgctacc tggccaccgc tctgctgacc ctgcagcaga tcgaactgaa
     5821 gttcaaccca cccgctctgc aggacgctta ctacagagcc cgtgctggcg aggccgctaa
     5881 cttctgcgct ctgatcctgg cctactgcaa caagaccgtg ggcgaactgg gagacgtgag
     5941 agagaccatg agctacctgt tccagcacgc caacctggac tcctgcaagc gtgtgctgaa
     6001 cgtggtgtgc aagacctgcg gccagcagca gaccaccctg aagggagtgg aagctgtgat
     6061 gtacatgggt accctgtcct acgagcagtt caagaagggc gtgcagatcc cttgcacctg
     6121 cggaaagcag gccaccaagt acctggtgca gcaggaaagc ccattcgtga tgatgtccgc
     6181 tcctccagcc cagtacgaac tgaagcacgg caccttcacc tgcgcttccg agtacaccgg
     6241 caactaccag tgcggacact acaagcacat cacctctaag gagaccctgt actgcatcga
     6301 cggagccctg ctgaccaagt cctctgaata caagggtccc atcaccgacg tgttctacaa
     6361 ggagaacagc tacaccacca ccatcaagcc cgtgacctac aagctggacg gcgtggtgtg
     6421 caccgaaatc gaccctaagc tggacaacta ctacaagaag gacaactctt acttcaccga
     6481 gcagcctatc gacctggtgc caaaccagcc ctaccctaac gccagcttcg acaacttcaa
     6541 gttcgtgtgc gacaacatca agttcgctga cgacctgaac cagctgaccg gatacaagaa
     6601 gcctgcctct agagagctga aggtgacctt cttcccagac ctgaacggcg acgtggtggc
     6661 tatcgactac aagcactaca ccccaagctt caagaaggga gccaagctgc tgcacaagcc
     6721 catcgtgtgg cacgtgaaca acgctaccaa caaggccacc tacaagccta acacctggtg
     6781 catccgttgc ctgtggtcta ccaagccagt ggaaacctcc aactctttcg acgtgctgaa
     6841 gagcgaggac gctcagggca tggacaacct ggcctgcgaa gacctgaagc ccgtgagcga
     6901 ggaagtggtg gagaacccaa ccatccagaa ggacgtgctg gaatgcaacg tgaagaccac
     6961 cgaggtggtg ggagacatca tcctgaagcc agctaacaac agcctgaaga tcaccgagga
     7021 agtgggtcac accgacctga tggccgctta cgtggacaac agctccctga ccatcaagaa
     7081 gcccaacgag ctgagcaggg tgctgggtct gaagaccctg gctactcacg gtctggctgc
     7141 tgtgaactct gtgccatggg acaccatcgc taactacgcc aagcctttcc tgaacaaggt
     7201 ggtgtccacc accaccaaca tcgtgaccag atgtctgaac cgcgtgtgca ccaactacat
     7261 gccctacttc ttcaccctgc tgctgcagct gtgcaccttc accagaagca ccaactcccg
     7321 tatcaaggct agcatgccta ccaccatcgc caagaacacc gtgaagagcg tgggaaagtt
     7381 ctgcctggag gcttccttca actacctgaa gtctccaaac ttcagcaagc tgatcaacat
     7441 catcatctgg ttcctgctgc tctccgtgtg cctgggttcc ctgatctact ctaccgccgc
     7501 tctgggagtg ctgatgtcta acctgggtat gcctagctac tgcactggtt acagagaggg
     7561 atacctgaac agcaccaacg tgaccatcgc cacctactgc accggcagca tcccatgctc
     7621 cgtgtgcctg tctggactgg acagcctgga cacctacccc tccctggaaa ccatccagat
     7681 caccatctct agcttcaagt gggacctgac cgccttcggt ctggtggctg agtggttcct
     7741 ggcctacatc ctgttcacca ggttcttcta cgtgctgggc ctggccgcta tcatgcagct
     7801 gttcttctcc tacttcgctg tgcacttcat cagcaactcc tggctgatgt ggctgatcat
     7861 caacctggtg cagatggctc ccatctctgc catggtgcgc atgtacatct tcttcgccag
     7921 cttctactac gtgtggaagt cctacgtgca cgtggtggac ggttgcaact cctctacctg
     7981 catgatgtgc tacaagcgca acagggctac cagggtggag tgcaccacca tcgtgaacgg
     8041 cgtgcgcagg tccttctacg tgtacgccaa cggcggcaag ggattctgca agctgcacaa
     8101 ctggaactgc gtgaactgcg acaccttctg cgctggctcc accttcatct ctgacgaggt
     8161 ggccagagac ctgagcctgc agttcaagcg tccaatcaac cccaccgacc agagctccta
     8221 catcgtggac tctgtgaccg tgaagaacgg tagcatccac ctgtacttcg acaaggctgg
     8281 ccagaagacc tacgagcgcc actctctgag ccacttcgtg aacctggaca acctgagggc
     8341 caacaacacc aagggcagcc tgcctatcaa cgtgatcgtg ttcgacggaa agtccaagtg
     8401 cgaggaatct agcgccaagt ccgccagcgt gtactacagc cagctgatgt gccagccaat
     8461 cctgctgctg gaccaggctc tggtgtctga cgtgggagac agcgccgaag tggccgtgaa
     8521 gatgttcgac gcctacgtga acaccttctc ctctaccttc aacgtgccta tggagaagct
     8581 gaagaccctg gtggctactg ctgaggctga actggctaag aacgtgtccc tggacaacgt
     8641 gctgagcacc ttcatctccg ccgctaggca gggtttcgtg gactctgacg tggaaaccaa
     8701 agacgtggtg gagtgcctga agctgtctca ccagagcgac atcgaagtga ccggcgactc
     8761 ctgcaacaac tacatgctga cctacaacaa ggtggagaac atgaccccaa gagacctggg
     8821 agcttgcatc gactgctccg cccgtcacat caacgctcag gtggccaagt ctcacaacat
     8881 cgccctgatc tggaacgtga aggacttcat gagcctgtcc gagcagctgc gcaagcagat
     8941 caggagcgcc gctaagaaga acaacctgcc cttcaagctg acctgcgcta ccaccagaca
     9001 ggtggtgaac gtggtgacca ccaagatcgc cctgaagggc ggataatacc caactttctt
     9061 gtacaaagtt gtgaaattct gcagatatcc atcacactgg cggccgctcg agcatgcatc
     9121 tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg
     9181 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     9241 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     9301 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     9361 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     9421 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     9481 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     9541 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     9601 ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     9661 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     9721 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     9781 ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa
     9841 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     9901 agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc atgcatctca
     9961 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    10021 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
    10081 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
    10141 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
    10201 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
    10261 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
    10321 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
    10381 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
    10441 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
    10501 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
    10561 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
    10621 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
    10681 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
    10741 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
    10801 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
    10861 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
    10921 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
    10981 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
    11041 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
    11101 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
    11161 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
    11221 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
    11281 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    11341 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
    11401 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    11461 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    11521 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    11581 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    11641 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    11701 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    11761 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    11821 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    11881 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    11941 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    12001 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    12061 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    12121 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    12181 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    12241 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
    12301 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    12361 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    12421 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    12481 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    12541 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    12601 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    12661 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    12721 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    12781 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    12841 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    12901 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    12961 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    13021 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    13081 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    13141 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    13201 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    13261 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    13321 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    13381 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    13441 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    13501 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    13561 ccgaaaagtg ccacctgacg tc
//
