LOCUS       pLenti-tetON-NKX        9792 bp DNA     circular SYN 23-JUN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9792)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jun 23, 2023 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     primer_bind     complement(859..878)
                     /label=SP_HIV_Rev
                     /note="color: black; sequence: ATATTTTTTCTTTCCCCCTG"
     primer_bind     1220..1241
                     /label=Up_RRE_For
                     /note="color: black; sequence: gtagtaaaaattgaaccattag"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    2217..2222
                     /label=AgeI site
                     /note="color: #ff99cc"
     promoter        2242..2616
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
                     /note="color: #99ccff"
     protein_bind    2246..2264
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2282..2300
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2318..2336
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2354..2372
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2390..2408
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2426..2444
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2462..2480
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     misc_feature    2493..2557
                     /label=Common to CMV
                     /note="color: #ff99cc"
     misc_feature    2617..2629
                     /label=FRT Repeat
                     /note="color: #00ffff"
     misc_feature    2631..2643
                     /label=FRT Repeat
                     /note="color: #00ffff"
     misc_feature    2644..2651
                     /label=FRT5 Spacer
                     /note="color: #3366ff"
     misc_feature    2652..2664
                     /label=FRT Inv Repeat
                     /note="color: #00ffff"
     misc_feature    2671..3786
                     /label=NKX2-1
                     /note="color: #00ccff"
     misc_feature    3152..3330
                     /label=DNA binding domain
                     /note="color: #0000ff"
     misc_feature    3193
                     /label=GAG Glu > TAG termination 523G>T: heterozygous
                     /note="color: #ff0000"
     polyA_site      3862..3917
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    4081..4086
                     /label=SpeI site
                     /note="color: #ff99cc"
     promoter        4087..4632
                     /locus_tag="EF1\promoter"
                     /label=EF1a promoter
                     /note="color: #ffcc99"
     CDS             4652..5362
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             5369..5422
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 5419"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5423..6169
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /note="color: #993366"
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6182..6770
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(6653..6664)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 6652"
                     /translation="IEGR"
     LTR             6845..7078
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        7283..7387
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             7388..8248
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7388 .. 7456 / #ccffcc / signal sequence
                        2: 7457 .. 8248 / #ccffcc
                     Cleavage site after base 7456"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7606..7625)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     rep_origin      8419..9007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     8908..8927
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     primer_bind     9161..9178
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     primer_bind     9270..9289
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     primer_bind     complement(9293..9304)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     protein_bind    9418..9439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        9454..9484
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 9454 .. 9459 / #ffffff / -35
                        2: 9460 .. 9477 / #ffffff
                        3: 9478 .. 9484 / #ffffff / -10"
     protein_bind    9492..9508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     9497..9519
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     primer_bind     9516..9532
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     9516..9532
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     9582..9601
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagaccg
     2221 gtggcctcca cggccactag attactccct atcagtgata gagaacgtat gaagagttta
     2281 ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt
     2341 ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt
     2401 gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat atccagttta
     2461 ctccctatca gtgatagaga acgtataagc tttaggcgtg tacggtgggc gcctataaaa
     2521 gcagagctcg tttagtgaac cgtcagatcg cctggagcaa ttccacaaca cttttgtctt
     2581 ataccaactt tccgtaccac ttcctaccct cgtaaagaag ttcctattcc gaagttccta
     2641 ttcttcaaaa ggtataggaa cttcgctcga atgtcgatga gtccaaagca cacgactccg
     2701 ttctcagtgt ctgacatctt gagtcccctg gaggaaagct acaagaaagt gggcatggag
     2761 ggcggcggcc tcggggctcc gctggcggcg tacaggcagg gccaggcggc accgccaaca
     2821 gcggccatgc agcagcacgc cgtggggcac cacggcgccg tcaccgccgc ctaccacatg
     2881 acggcggcgg gggtgcccca gctctcgcac tccgccgtgg ggggctactg caacggcaac
     2941 ctgggcaaca tgagcgagct gccgccgtac caggacacca tgaggaacag cgcctctggc
     3001 cccggatggt acggcgccaa cccagacccg cgcttccccg ccatctcccg cttcatgggc
     3061 ccggcgagcg gcatgaacat gagcggcatg ggcggcctgg gctcgctggg ggacgtgagc
     3121 aagaacatgg ccccgctgcc aagcgcgccg cgcaggaagc gccgggtgct cttctcgcag
     3181 gcgcaggtgt actagctgga gcgacgcttc aagcaacaga agtacctgtc ggcgccggag
     3241 cgcgagcacc tggccagcat gatccacctg acgcccacgc aggtcaagat ctggttccag
     3301 aaccaccgct acaaaatgaa gcgccaggcc aaggacaagg cggcgcagca gcaactgcag
     3361 caggacagcg gcggcggcgg gggcggcggg ggcaccgggt gcccgcagca gcaacaggct
     3421 cagcagcagt cgccgcgacg cgtggcggtg ccggtcctgg tgaaagacgg caaaccgtgc
     3481 caggcgggtg cccccgcgcc gggcgccgcc agcctacaag gccacgcgca gcagcaggcg
     3541 cagcaccagg cgcaggccgc gcaggcggcg gcagcggcca tctccgtggg cagcggtggc
     3601 gccggccttg gcgcacaccc gggccaccag ccaggcagcg caggccagtc tccggacctg
     3661 gcgcaccacg ccgccagccc cgcggcgctg cagggccagg tatccagcct gtcccacctg
     3721 aactcctcgg gctcggacta cggcaccatg tcctgctcca ccttgctata cggtcggacc
     3781 tggtgaggcc gcctaatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     3841 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     3901 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     3961 atgagtattt ggtttagagt ttggcaacat atgccatatg ctggctgcca tgaacaaagg
     4021 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttacatatg
     4081 actagtaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     4141 agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg
     4201 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     4261 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     4321 cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac
     4381 ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc
     4441 ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc
     4501 ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct
     4561 tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg
     4621 accggcgcct acacctgcag ccaacgccac catggtgtct aagggcgaag agctgattaa
     4681 ggagaacatg cacatgaagc tgtacatgga gggcaccgtg aacaaccacc acttcaagtg
     4741 cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt
     4801 cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca tgtacggcag
     4861 cagaaccttc atcaaccaca cccagggcat ccccgacttc tttaagcagt ccttccctga
     4921 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca
     4981 ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt
     5041 cccatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcca acaccgagat
     5101 gctgtacccc gctgacggcg gcctggaagg cagaagcgac atggccctga agctcgtggg
     5161 cgggggccac ctgatctgca acttcaagac cacatacaga tccaagaaac ccgctaagaa
     5221 cctcaagatg cccggcgtct actatgtgga ccacagactg gaaagaatca aggaggccga
     5281 caaagagacc tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag
     5341 caaactgggg cacaaactta atgctagcga gggcagagga agtctgctaa catgcggtga
     5401 cgtcgaggag aatcctggcc caatgtctag actggacaag agcaaagtca taaactctgc
     5461 tctggaatta ctcaatggag tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa
     5521 gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga
     5581 tgccctgcca atcgagatgc tggacaggca tcatacccac tcctgccccc tggaaggcga
     5641 gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca
     5701 tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct
     5761 ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc
     5821 tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt
     5881 agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc tgaaacaagc
     5941 aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat
     6001 catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga
     6061 cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct
     6121 gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaag tcgacgatcc
     6181 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6241 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6301 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6361 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6421 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6481 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     6541 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     6601 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     6661 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     6721 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct
     6781 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     6841 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     6901 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6961 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7021 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7081 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     7141 agtgagaggc ccgggttaat taaggaaagg gctagatcat tcttgaagac gaaagggcct
     7201 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     7261 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7321 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7381 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7441 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7501 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7561 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7621 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7681 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7741 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7801 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7861 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7921 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7981 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8041 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     8101 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     8161 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8221 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8281 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8341 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8401 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8461 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8521 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8581 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8641 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8701 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8761 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8821 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8881 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8941 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9001 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9061 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     9121 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     9181 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     9241 cagcaagctc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9301 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     9361 ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     9421 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     9481 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac
     9541 gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     9601 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
     9661 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
     9721 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
     9781 aaacttagta gt
//
