LOCUS       pLenti-tetON-NKX        9613 bp DNA     circular SYN 10-JUN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2023 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9613
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        2242..2616
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
                     /note="color: #99ccff"
     protein_bind    2246..2264
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2282..2300
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2318..2336
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2354..2372
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2390..2408
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2426..2444
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2462..2480
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     misc_feature    2493..2557
                     /label=Common to CMV
                     /note="color: #ff99cc"
     misc_feature    2617..2629
                     /label=FRT Repeat
                     /note="color: #00ffff"
     misc_feature    2631..2643
                     /label=FRT Repeat
                     /note="color: #00ffff"
     misc_feature    2644..2651
                     /label=FRT5 Spacer
                     /note="color: #3366ff"
     misc_feature    2652..2664
                     /label=FRT Inv Repeat
                     /note="color: #00ffff"
     misc_feature    2671..3607
                     /label=NKX2-1
                     /note="This feature has 2 segments:
                        1: 2671 .. 3151 / #00ccff
                        2: 3152 .. 3607 / #00ccff"
     polyA_site      3683..3738
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        3908..4453
                     /locus_tag="EF1\promoter"
                     /label=EF1a promoter
                     /note="color: #ffcc99"
     CDS             4473..5183
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             5190..5243
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 5240"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5244..5990
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /note="color: #993366"
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6003..6591
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(6474..6485)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 6473"
                     /translation="IEGR"
     LTR             6666..6899
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     complement(7018..7036)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /note="color: black; sequence: AATAGGCGTATCACGAGGC"
     promoter        7104..7208
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             7209..8069
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7209 .. 7277 / #ccffcc / signal sequence
                        2: 7278 .. 8069 / #ccffcc
                     Cleavage site after base 7277"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7427..7446)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     rep_origin      8240..8828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     8729..8748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     primer_bind     8982..8999
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     primer_bind     9091..9110
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     primer_bind     complement(9114..9125)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     protein_bind    9239..9260
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        9275..9305
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 9275 .. 9280 / #ffffff / -35
                        2: 9281 .. 9298 / #ffffff
                        3: 9299 .. 9305 / #ffffff / -10"
     protein_bind    9313..9329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     9318..9340
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     primer_bind     9337..9353
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     9337..9353
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     9403..9422
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagaccg
     2221 gtggcctcca cggccactag attactccct atcagtgata gagaacgtat gaagagttta
     2281 ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt
     2341 ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt
     2401 gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat atccagttta
     2461 ctccctatca gtgatagaga acgtataagc tttaggcgtg tacggtgggc gcctataaaa
     2521 gcagagctcg tttagtgaac cgtcagatcg cctggagcaa ttccacaaca cttttgtctt
     2581 ataccaactt tccgtaccac ttcctaccct cgtaaagaag ttcctattcc gaagttccta
     2641 ttcttcaaaa ggtataggaa cttcgctcga atgtcgatga gtccaaagca cacgactccg
     2701 ttctcagtgt ctgacatctt gagtcccctg gaggaaagct acaagaaagt gggcatggag
     2761 ggcggcggcc tcggggctcc gctggcggcg tacaggcagg gccaggcggc accgccaaca
     2821 gcggccatgc agcagcacgc cgtggggcac cacggcgccg tcaccgccgc ctaccacatg
     2881 acggcggcgg gggtgcccca gctctcgcac tccgccgtgg ggggctactg caacggcaac
     2941 ctgggcaaca tgagcgagct gccgccgtac caggacacca tgaggaacag cgcctctggc
     3001 cccggatggt acggcgccaa cccagacccg cgcttccccg ccatctcccg cttcatgggc
     3061 ccggcgagcg gcatgaacat gagcggcatg ggcggcctgg gctcgctggg ggacgtgagc
     3121 aagaacatgg ccccgctgcc aagcgcgccg caaggacaag gcggcgcagc agcaactgca
     3181 gcaggacagc ggcggcggcg ggggcggcgg gggcaccggg tgcccgcagc agcaacaggc
     3241 tcagcagcag tcgccgcgac gcgtggcggt gccggtcctg gtgaaagacg gcaaaccgtg
     3301 ccaggcgggt gcccccgcgc cgggcgccgc cagcctacaa ggccacgcgc agcagcaggc
     3361 gcagcaccag gcgcaggccg cgcaggcggc ggcagcggcc atctccgtgg gcagcggtgg
     3421 cgccggcctt ggcgcacacc cgggccacca gccaggcagc gcaggccagt ctccggacct
     3481 ggcgcaccac gccgccagcc ccgcggcgct gcagggccag gtatccagcc tgtcccacct
     3541 gaactcctcg ggctcggact acggcaccat gtcctgctcc accttgctat acggtcggac
     3601 ctggtgaggc cgcctaatct ttttccctct gccaaaaatt atggggacat catgaagccc
     3661 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
     3721 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
     3781 aatgagtatt tggtttagag tttggcaaca tatgccatat gctggctgcc atgaacaaag
     3841 gtggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttacatat
     3901 gactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     3961 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     4021 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     4081 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     4141 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     4201 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     4261 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     4321 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     4381 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     4441 gaccggcgcc tacacctgca gccaacgcca ccatggtgtc taagggcgaa gagctgatta
     4501 aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt
     4561 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg
     4621 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca
     4681 gcagaacctt catcaaccac acccagggca tccccgactt ctttaagcag tccttccctg
     4741 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc
     4801 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact
     4861 tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc aacaccgaga
     4921 tgctgtaccc cgctgacggc ggcctggaag gcagaagcga catggccctg aagctcgtgg
     4981 gcgggggcca cctgatctgc aacttcaaga ccacatacag atccaagaaa cccgctaaga
     5041 acctcaagat gcccggcgtc tactatgtgg accacagact ggaaagaatc aaggaggccg
     5101 acaaagagac ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta
     5161 gcaaactggg gcacaaactt aatgctagcg agggcagagg aagtctgcta acatgcggtg
     5221 acgtcgagga gaatcctggc ccaatgtcta gactggacaa gagcaaagtc ataaactctg
     5281 ctctggaatt actcaatgga gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa
     5341 agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg
     5401 atgccctgcc aatcgagatg ctggacaggc atcataccca ctcctgcccc ctggaaggcg
     5461 agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac
     5521 atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc
     5581 tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg
     5641 ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag
     5701 tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt ctgaaacaag
     5761 caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa
     5821 tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg
     5881 acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc
     5941 tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa gtcgacgatc
     6001 ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6061 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6121 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6181 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6241 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6301 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6361 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     6421 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     6481 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     6541 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc
     6601 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     6661 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     6721 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6781 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6841 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6901 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     6961 gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc
     7021 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     7081 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     7141 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     7201 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     7261 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7321 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     7381 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     7441 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7501 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7561 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7621 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7681 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7741 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7801 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7861 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7921 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7981 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     8041 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     8101 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     8161 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8221 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8281 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8341 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     8401 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8461 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8521 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8581 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8641 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8701 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8761 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8821 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8881 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8941 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     9001 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     9061 gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     9121 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     9181 gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     9241 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     9301 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
     9361 cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9421 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
     9481 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
     9541 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     9601 caaacttagt agt
//
