LOCUS       Exported                6394 bp ds-DNA     circular SYN 12-JUN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 12, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        232..819
                     /locus_tag="CMV\promotor"
                     /label=CMV\promotor
                     /note="/vntifkey=29"
     primer_bind     769..789
                     /locus_tag="CMV\forward\priming\site"
                     /label=CMV\forward\priming\site
                     /note="/vntifkey=28"
     misc_feature    769..789
                     /locus_tag="CMV\fwd\primer"
                     /label=CMV\fwd\primer
                     /note="/vntifkey=21"
     primer_bind     863..882
                     /locus_tag="T7\promotor/priming\site"
                     /label=T7\promotor/priming\site
                     /note="/vntifkey=28"
     CDS             901..1833
                     /locus_tag="Halo\TEV"
                     /label=Halo\TEV
                     /note="/vntifkey=21"
     misc_feature    904..906
                     /locus_tag="start"
                     /label=start
     misc_feature    1807..1827
                     /locus_tag="TEV site"
                     /label=TEV site
     misc_feature    1834..1841
                     /locus_tag="PacI"
                     /label=PacI
                     /note="/vntifkey=21"
     misc_feature    1848..1878
                     /locus_tag="10 AA linker"
                     /label=10 AA linker
     misc_feature    1878
                     /locus_tag="needed in linker"
                     /label=needed in linker
     misc_feature    1879..2301
                     /locus_tag="Profilin1"
                     /label=Profilin1
     misc_feature    1898..1899
                     /locus_tag="Y6  TAC for Y6D change first T to G GAC = D"
                     /label=Y6  TAC for Y6D change first T to G GAC =
                     D
                     /label=Y6  TAC for Y6D change first T to G GAC = D
     misc_feature    2143..2145
                     /locus_tag="R89 CGT change to GAG for R89E"
                     /label=R89 CGT change to GAG for R89E
     misc_feature    2230..2232
                     /locus_tag="G118 GGT change to middle G to T GTT for
                     G118V"
                     /label=G118 GGT change to middle G to T GTT for
                     G118V
                     /label=G118 GGT change to middle G to T GTT for G118V
     misc_feature    2290..2292
                     /locus_tag="S137 TCC change first T to G for GCC for
                     S137A"
                     /label=S137 TCC change first T to G for GCC for
                     S137A
                     /label=S137 TCC change first T to G for GCC for S137A
     misc_feature    2302..2309
                     /locus_tag="NotI"
                     /label=NotI
     primer_bind     2346..2363
                     /locus_tag="BGH\reverse\priming\site"
                     /label=BGH\reverse\priming\site
                     /note="/vntifkey=28"
     polyA_signal    2352..2576
                     /locus_tag="BGH\polyadenylation\signal"
                     /label=BGH\polyadenylation\signal
                     /note="/vntifkey=25"
     misc_recomb     2860..2907
                     /locus_tag="FRT\site"
                     /label=FRT\site
                     /note="/vntifkey=86"
     misc_feature    2915..3935
                     /locus_tag="Hygromycin\resistance\gene\(no\ATG)"
                     /label=Hygromycin\resistance\gene\(no\ATG)
                     /label=nonstandard type:  misc_marker
                     /note="/vntifkey=22"
     polyA_signal    4067..4197
                     /locus_tag="SV40\early\polyadenylation\signal"
                     /label=SV40\early\polyadenylation\signal
                     /note="/vntifkey=25"
     rep_origin      complement(4580..5253)
                     /direction=LEFT
                     /locus_tag="pUC\origin"
                     /label=pUC\origin
                     /note="/vntifkey=33"
     misc_feature    complement(5398..6258)
                     /locus_tag="Ampicillin\(bla)\resistance\gene"
                     /label=Ampicillin\(bla)\resistance\gene
                     /label=nonstandard type:  misc_marker
                     /note="/vntifkey=22"
     promoter        complement(6259..6357)
                     /locus_tag="bla\promoter"
                     /label=bla\promoter
                     /note="/vntifkey=30"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 atcatggcag aaatcggtac tggctttcca ttcgaccccc attatgtgga agtcctgggc
      961 gagcgcatgc actacgtcga tgttggtccg cgcgatggca cccctgtgct gttcctgcac
     1021 ggtaacccga cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc accgacccat
     1081 cgctgcattg ctccagacct gatcggtatg ggcaaatccg acaaaccaga cctgggttat
     1141 ttcttcgacg accacgtccg cttcatggat gccttcatcg aagccctggg tctggaagag
     1201 gtcgtcctgg tcattcacga ctggggctcc gctctgggtt tccactgggc caagcgcaat
     1261 ccagagcgcg tcaaaggtat tgcatttatg gagttcatcc gccctatccc gacctgggac
     1321 gaatggccag aatttgcccg cgagaccttc caggccttcc gcaccaccga cgtcggccgc
     1381 aagctgatca tcgatcagaa cgtttttatc gagggtacgc tgccgatggg tgtcgtccgc
     1441 ccgctgactg aagtcgagat ggaccattac cgcgagccgt tcctgaatcc tgttgaccgc
     1501 gagccactgt ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc gaacatcgtc
     1561 gcgctggtcg aagaatacat ggactggctg caccagtccc ctgtcccgaa gctgctgttc
     1621 tggggcaccc caggcgttct gatcccaccg gccgaagccg ctcgcctggc caaaagcctg
     1681 cctaactgca aggctgtgga catcggcccg ggtctgaatc tgctgcaaga agacaacccg
     1741 gacctgatcg gcagcgagat cgcgcgctgg ctgtcgacgc tcgagatttc cggcgagcca
     1801 accactgagg atctgtactt tcagagcgat aacttaatta aggtacctcc ggactcagat
     1861 ctcgagctca agcttccaat ggccgggtgg aacgccgaca tcgacaacct catggcggac
     1921 gggacctgtc aggacgcggc catcgtgggc tacaaggact cgccctccgt ctgggccgcc
     1981 gtccccggga aaacgttcgt caacatcacg ccagctgagg tgggtgtcct ggttggcaaa
     2041 gaccggtcaa gtttttacgt gaatgggctg acacttgggg gccagaaatg ttcggtgatc
     2101 cgggactcac tgctgcagga tggggaattt agcatggatc ttcgtaccaa gagcaccggt
     2161 ggggccccca ccttcaatgt cactgtcacc aagactgaca agacgctagt cctgctgatg
     2221 ggcaaagaag gtgtccacgg tggtttgatc aacaagaaat gttatgaaat ggcctcccac
     2281 cttcggcgtt cccagtactg agcggccgct cgagtctaga gggcccgttt aaacccgctg
     2341 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     2401 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     2461 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     2521 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     2581 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     2641 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     2701 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     2761 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     2821 ccccaaaaaa cttgattagg gtgatggttc acgtacctag aagttcctat tccgaagttc
     2881 ctattctcta gaaagtatag gaacttcctt ggccaaaaag cctgaactca ccgcgacgtc
     2941 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     3001 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     3061 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     3121 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     3181 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     3241 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     3301 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     3361 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     3421 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     3481 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     3541 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     3601 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     3661 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     3721 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     3781 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     3841 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     3901 ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac gtactacgag atttcgattc
     3961 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     4021 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     4081 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     4141 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     4201 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     4261 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     4321 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     4381 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     4441 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4501 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4561 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4621 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4681 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4741 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4801 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4861 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4921 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4981 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5041 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     5101 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5161 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5221 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5281 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     5341 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     5401 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     5461 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     5521 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     5581 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5641 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5701 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     5761 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     5821 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     5881 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     5941 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     6001 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     6061 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     6121 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     6181 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     6241 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     6301 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     6361 gcgcacattt ccccgaaaag tgccacctga cgtc
//
