LOCUS       pJAT39{U6-3-attR3-Amp-ccdB-attR4-MHC-EGFP-attR5-Chlor-SacB-attR6-U6-1}       10998 bp    DNA     circular UNA 12-JUN-2023
DEFINITION  Ligation of 2 sequences.
ACCESSION   urn.local.sternd.1nj-gdrcq5h
VERSION     urn.local.sternd.1nj-gdrcq5h
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..30
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     31..50
                     /standard_name="pGGAselect PCR Product (270 R) - pGWB402
                     (digested by PsyI, SapI) Fragment 4"
     regulatory      43..61
                     /note="Geneious type: Promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7 prom"
     misc_feature    74..103
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    74..103
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      104..907
                     /note="Geneious type: promoter"
                     /standard_name="U6:1 promoter"
     misc_feature    167
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="C"
     misc_feature    205
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="T"
     misc_feature    215
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="T"
     misc_feature    224
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="T"
     misc_feature    242
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="T"
     primer_bind     complement(436..456)
                     /standard_name="attB F seq"
     misc_feature    908..937
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="GGGTCTTCGAGAAGAC"
     misc_feature    908..937
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    926..929
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     930..954
                     /standard_name="1,219 R"
     misc_feature    960..1083
                     /note="Geneious type: attR6"
                     /standard_name="attR6"
     gene            complement(1121..2543)
                     /note="counterselectable marker from Bacillus subtilis
                     that converts sucrose to levans"
                     /product="levansucrase"
                     /gene="sacB"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB CS"
     misc_feature    1123..2995
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CAAATGCCCCAGAACATCAGGTTAATTGCATTTTTGATGTCA
                     TTTTCACGGTGAGACAGATCGGCGACTTCCTCGCCAATGACGCTAACCGGGACAGAAG
                     CCATGTCAGTCGTCATCATGCGCCAAGACTCGTCGCCAATGTGTACAACCGGATACAG
                     TTCACGGCTAACTTTATCAGACAGGAGACGTGCAGATGCCAGCGGAATAACCATACGG
                     CGGCCCGGCGTATCAATGATATCAGACTGTACGTCTACAAACAGACGGTAGCGAGATT
                     CGCGCTTGTAGGTATAAACTTTAAATTGCATTTCACCAGCCCCTGTTCTCGTCAGCAA
                     AAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGC
                     TTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGA
                     CCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGT
                     CACTGTAATACGCTGCTTCATAGCATACCTCTTTTTGACATACTTCGGGTATACATAT
                     CAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGC
                     TTTTAGTAAGCCGGATCAAATGCCCCAGAACATCAGGTTAATTGCATTTTTGATGTCA
                     TTTTCACGGTGAGACAGATCGGCGACTTCCTCGCCAATGACGCTAACCGGGACAGAAG
                     CCATGTCAGTCGTCATCATGCGCCAAGACTCGTCGCCAATGTGTACAACCGGATACAG
                     TTCACGGCTAACTTTATCAGACAGGAGACGTGCAGATGCCAGCGGAATAACCATACGG
                     CGGCCCGGCGTATCAATGATATCAGACTGTACGTCTACAAACAGACGGTAGCGAGATT
                     CGCGCTTGTAGGTATAAACTTTAAATTGCATTTCACCAGCCCCTGTTCTCGTCAGCAA
                     AAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGC
                     TTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGA
                     CCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGT
                     CACTGTAATACGCTGCTTCATAGCATACCTCTTTTTGACATACTTCGGGTATACATAT
                     CAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGC
                     TTTTAGTAAGCCGGATCCACGCGTTTACGCACCGCCCTGCCATTCATCACAGTATTGC
                     TGCAGCTCGTTCAGCATGCGACCGACATGGAAGCCGTCACATACCGCGTGATGCACCT
                     GGATTGCCAGCGGCATCAGTACCTTGTCACCCTGGGTGTAATACTTGCCCATCGTGAA
                     AACCGGCGCGAAAAAGTTGTCCATGTTCGCCACGTTCAGGTCGAAAGAAGTGAAGCTC
                     ACCCATGGGTTGGCGCTAACAAAGAACATGTTTTCAATGAAACCCTTCGGAAAATAAG
                     CGAGATTCTCACCATAGCACGCAACGTCCTGAGAATAAATGTGCAGGAATTGACGGAA
                     GTCATCGTGATATTCAGACCACAGGGAAGAGAACGTCTCG"
     primer_bind     complement(2126..2148)
                     /standard_name="1 F"
     misc_feature    2149..2152
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     2149..2169
                     /standard_name="841 R"
     regulatory      complement(2544..2989)
                     /note="Geneious type: promoter"
                     /note="Bacillus subtilis sacB (glycoside hydrolase family
                     68) promoter region"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB"
     primer_bind     complement(2969..2989)
                     /standard_name="1 F"
     misc_feature    2990..2993
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     2990..3006
                     /standard_name="905 R"
     gene            complement(2998..3657)
                     /gene="CAT/CamR"
                     /note="Geneious type: CDS"
                     /standard_name="CAT/CamR"
     misc_feature    3000..3652
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CAGACCACAGGGAAGAGAACGTCTCGGTCTGTTCGTGAAAGA
                     CGGTATAACACGGATGAACGGAATCCCAAATTACCAGCTCGCCATCTTTCATGGCCAT
                     ACGGAATTCCGGGTGTGCGTTCATCAGGCGAGCGAGAATGTGGATAAAAGCCGGATAA
                     AATTTGTGTTTGTTTTTCTTAACAGTCTTCAGGAAAGCAGTGATATCCAGCTGAACGG
                     TCTGATTGTAGGTGCACTGCGCGACGCTCTGGAACGCTTCGAAATGTTCTTTACGGTG
                     CCACTGGCTGATGTCGACGGTAGTATAACCCGTAATCTTTTTT"
     misc_feature    complement(3764..3887)
                     /note="Geneious type: attR5"
                     /standard_name="attR5"
     misc_feature    3884..3887
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    3891..4568
                     /note="Geneious type: regulatory_region"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Mhc_F4-678"
     primer_bind     3891..3912
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="3523R"
     primer_bind     complement(4008..4027)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="2647R"
     misc_feature    4569..4593
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    complement(4573..4592)
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="4558 R"
     regulatory      4579..>4736
                     /note="Geneious type: Promoter"
                     /standard_name="HS prom"
     misc_feature    complement(4617..4635)
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="12878 R"
     misc_feature    complement(4617..4635)
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2020.2.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="P693
                     p(MHC-DsRed-CDNopt-5'myr-smGFP-int-B3-B3 recombinase-FSF)
                     - 12878 R (reversed)"
     misc_feature    <4733..4759
                     /note="Geneious type: Inserted sequence"
                     /standard_name="Mhc_F4-678-promoter"
     misc_feature    4733..6038
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P404 pBac{Mhc678-GFP,w+,attB}  (digested
                     by NotI, MfeI) Fragment 2 (concatenated sequence 2)"
     misc_feature    4733..4736
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     source          <4760..>6038
                     /organism="pJFRC12-10XUAS-IVS-myr::GFP"
                     /mol_type="other DNA"
     misc_feature    4761..5474
                     /note="Geneious type: Reporter"
                     /standard_name="EGFP reporter"
     gene            5569..5628
                     /note="Geneious type: Gene"
                     /standard_name="SV40_3_splice"
     regulatory      5941..>6038
                     /note="Geneious type: Terminator"
                     /standard_name="SV40_PA term"
     misc_feature    complement(5988..6007)
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40-PA-R"
     misc_feature    6035..4736
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P929
                     p{dU6-3-GW1-Amp-ccdB-Mhc-dsRed-GW2-Chlor-SacB-U6-1}
                     (digested by MfeI, Bsp120I) Fragment 2 (concatenated
                     sequence 1)"
     regulatory      <6035..6155
                     /note="Geneious type: Terminator"
                     /standard_name="SV40_PA term"
     misc_feature    6035..6038
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     6075..6094
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40-PA-F"
     primer_bind     6123..6143
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="1198F"
     misc_feature    6123^6124
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="CACTGCATTCTAGTTGTGGAGCATTTTTTC"
     primer_bind     6190..6209
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="8,388 F"
     primer_bind     6218..6237
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="2655R"
     misc_feature    complement(<6237..6243)
                     /locus_tag="ie1 promoter"
                     /note="Geneious type: Enhancer"
                     /standard_name="ie1"
     misc_feature    6237..6240
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    6244..6247
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    6251..6374
                     /note="Geneious type: attR4"
                     /standard_name="attR4"
     gene            complement(6412..6744)
                     /standard_name="ccdB"
     misc_feature    6414..7053
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TAAATACCCCAAAACATGAGATTGATTGCATTCTTAATATCA
                     TTCTCACGGTGGCTTAAATCCGCGACCTCTTCCCCGATCACCGACACAGGTACACTAG
                     CCATGTCTGTAGTCATCATGCGCCAACTCTCGTCACCAATATGGACGACTGGGTAAAG
                     CTCGCGCGACACCTTATCACTTAACAGGCGTGCGCTGGCTAATGGAATCACCATACGG
                     CGACCAGGCGTGTCGATAATGTCTGACTGGACGTCCACGAAAAGGCGATAGCGGGACT
                     CGCGCTTGTAAGTATAAACCTTAAATTGCATTTCACCAGCCCCTGTTCTCGTCAGCAA
                     AAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGC
                     TTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGA
                     CCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGT
                     CACTGTAATACGCTGCT"
     primer_bind     complement(6534..6553)
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="4446F"
     primer_bind     complement(7032..7053)
                     /standard_name="1 F"
     misc_feature    complement(7054..7914)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     misc_feature    7054..7057
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     7054..7077
                     /standard_name="966 R"
     misc_feature    complement(7067..7090)
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp_3'_R"
     primer_bind     7163..7182
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_F"
     misc_feature    7195
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     misc_feature    complement(7649..7668)
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_R"
     primer_bind     7853..7874
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AMP_5'_F"
     regulatory      complement(7915..8019)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     primer_bind     complement(8000..8019)
                     /standard_name="1 F"
     misc_feature    8020..8023
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     8020..8041
                     /standard_name="676 R"
     misc_feature    complement(8115..8238)
                     /note="Geneious type: attR3"
                     /standard_name="attR3"
     misc_feature    8212..8241
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      complement(8245..8665)
                     /note="Geneious type: promoter"
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
                     /standard_name="dU6-3 promoter"
     primer_bind     complement(8677..8695)
                     /standard_name="1 F"
     misc_feature    8696..8699
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      complement(8741..8760)
                     /note="Geneious type: promoter"
                     /note="SP6 RNA polymerase promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SP6"
     primer_bind     complement(8770..8789)
                     /standard_name="pGGAselect PCR Product (1 F) - pGWB402
                     (digested by PsyI, SapI) Fragment 4"
     misc_feature    8790..8819
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     complement(8792..8809)
                     /note="L4440 vector, forward primer"
                     /standard_name="L4440"
     primer_bind     8928..8947
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp-ori-7571F"
     primer_bind     complement(8954..8973)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7Cas9F"
     rep_origin      complement(8963..9551)
     primer_bind     complement(9043..9062)
                     /note="pBR322 origin, forward primer"
                     /standard_name="pBR322ori-F"
     misc_feature    complement(9162..9179)
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ori-252 R"
     CDS             complement(9797..10588)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /standard_name="SmR"
ORIGIN      
        1 tgccgcctta caacggctct cccgctgacg cgcccgcgaa attaatacga ctcactatag
       61 ggagactcac atgcggccgc ctcgagaatt ctgacgtctt aattgaattc attttcaacg
      121 tcctcgatag tatagtggtt agtatccccg cctgtcacgc gggagaacgg ggttcaattc
      181 cccgtcgggg agaatctgtg attccttttt ttttgctttt actctgttat ataaacaatt
      241 tctgttttaa ttgaatctaa tttgccattg cttttaggaa tctcaggcat ccagcaagcg
      301 tttgtccgcc gaatcgccca tcagtgaaga agatcctgtg gcggctacga aaatctcccc
      361 ggccatgtcg gcctccacct ccagcgaaaa acccatcagc gagctggcca cctctgtgct
      421 gacccaccgc tttccagact ccacctcctc acccggcgaa catggccttg gacgaatgca
      481 gttgtcgatc cgctacagcg cccagcgtca aaaactagac gtgaccatac acaaaatcca
      541 gaagatacca cttcgcgatc ccagcaatat ccccgatccg tatgttaagc tgtatctgtt
      601 gcctggacgc accaaggagt cgaaacgcaa gacgagcgtg atcaaggaca actgcaaccc
      661 cgtctacgat gcatcctttg agtacctgat ttccattgcc gaactcaggc agacggaact
      721 ggaggtgacg gtgtgcaccc aaaagggatt cctatccggc ggtagtccca tcattggcat
      781 ggtaggtacc cgaaagcaac cccttagtta cagacacagc gcgtacgtcc ttcgcatcct
      841 tatgattccc aagtacatat tctgcaagag tacagtatat ataggaaaga tatccgggtg
      901 aacttcgatt aagaagtcta caggaatggg catctagatg caccattatg ttccggaggc
      961 aactttgtat taaaaagttg aacgagaaac gtaaaatgat ataaatatca atatattaaa
     1021 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
     1081 atgcgacgtg cagactggct gtgtataagg gagcctgaca tttatttgtt aactgttaat
     1141 tgtccttgtt caaggatgct gtctttgaca acagatgttt tcttgccttt gatgttcagc
     1201 aggaagcttg gcgcaaacgt tgattgtttg tctgcgtaga atcctctgtt tgtcatatag
     1261 cttgtaatca cgacattgtt tcctttcgct tgaggtacag cgaagtgtga gtaagtaaag
     1321 gttacatcgt taggatcaag atccattttt aacacaaggc cagttttgtt cagcggcttg
     1381 tatgggccag ttaaagaatt agaaacataa ccaagcatgt aaatatcgtt agacgtaatg
     1441 ccgtcaatcg tcatttttga tccgcgggag tcagtgaaca ggtaccattt gccgttcatt
     1501 ttaaagacgt tcgcgcgttc aatttcatct gttactgtgt tagatgcaat cagcggtttc
     1561 atcacttttt tcagtgtgta atcatcgttt agctcaatca taccgagagc gccgtttgct
     1621 aactcagccg tgcgtttttt atcgctttgc agaagttttt gactttcttg acggaagaat
     1681 gatgtgcttt tgccatagta tgctttgtta aataaagatt cttcgccttg gtagccatct
     1741 tcagttccag tgtttgcttc aaatactaag tatttgtggc ctttatcttc tacgtagtga
     1801 ggatctctca gcgtatggtt gtcgcctgag ctgtagttgc cttcatcgat gaactgctgt
     1861 acattttgat acgtttttcc gtcaccgtca aagattgatt tataatcctc tacaccgttg
     1921 atgttcaaag agctgtctga tgctgatacg ttaacttgtg cagttgtcag tgtttgtttg
     1981 ccgtaatgtt taccggagaa atcagtgtag aataaacgga tttttccgtc agatgtaaat
     2041 gtggctgaac ctgaccattc ttgtgtttgg tcttttagga tagaatcatt tgcatcgaat
     2101 ttgtcgctgt ctttaaagac gcggccagcg tttttccagc tgtcaataga agtttcgccg
     2161 actttttgat agaacatgta aatcgatgtg tcatccgcat ttttaggatc tccggctaat
     2221 gcaaagacga tgtggtagcc gtgatagttt gcgacagtgc cgtcagcgtt ttgtaatggc
     2281 cagctgtccc aaacgtccag gccttttgca gaagagatat ttttaattgt ggacgaatcg
     2341 aattcaggaa cttgatattt ttcatttttt tgctgttcag ggatttgcag catatcatgg
     2401 cgtgtaatat gggaaatgcc gtatgtttcc ttatatggct tttggttcgt ttctttcgca
     2461 aacgcttgag ttgcgcctcc tgccagcagt gcggtagtaa aggttaatac tgttgcttgt
     2521 tttgcaaact ttttgatgtt catcgttcat gtctcctttt ttatgtactg tgttagcggt
     2581 ctgcttcttc cagccctcct gtttgaagat ggcaagttag ttacgcacaa taaaaaaaga
     2641 cctaaaatat gtaaggggtg acgccaaagt atacactttg ccctttacac attttaggtc
     2701 ttgcctgctt tatcagtaac aaacccgcgc gatttacttt tcgacctcat tctattagac
     2761 tctcgtttgg attgcaactg gtctattttc ctcttttgtt tgatagaaaa tcataaaagg
     2821 atttgcagac tacgggccta aagaactaaa aaatctatct gtttcttttc attctctgta
     2881 ttttttatag tttctgttgc atgggcataa agttgccttt ttaatcacaa ttcagaaaat
     2941 atcataatat ctcatttcac taaataatag tgaacggcag gtatatgtgc cacgcgttta
     3001 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     3061 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     3121 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     3181 gtttaaatca aaactggtga aactcaccca gggattggct gacacgaaaa acatattctc
     3241 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     3301 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     3361 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     3421 gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa gaatgtgaat
     3481 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     3541 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     3601 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     3661 agcttcctta gctcctgaaa atctcgacgg atcctaactc aaaatccaca cattatacga
     3721 gccggaagca taaagtgtaa agcctggggt gcctaatgcg gcccatagtg actggatatg
     3781 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     3841 atttatatca ttttacgttt ctcgttcaac ttttgtatac aaagttgatt atactcgaat
     3901 tcgttgtcgg ctcatataca tgggcgaaat aatttcgaat atgttttaaa aataaccaaa
     3961 gacattagaa agagatcgcc aatacttata cattatgtct atgtgtgcca tgtggtagca
     4021 tgagccaaaa agcttctcga aattacgaat tacatataga cgaatatgta tgtgacttta
     4081 gtttcgaaat aatttcgaga attttaaaaa taacgcattc gttaaaagtt cgcgtcaatt
     4141 cgaatcgaat tttcgattgt cgatttagtg tggattgtcg aaaatcgttc cgccttcgaa
     4201 gtttactgaa aggaatcatt gcgatctcgt gaattgcttg tatgagaaca cgccaccata
     4261 tcgagatacc tgttcaaaat atcgaaatcg cgttccaaac ggtagtagtt accagttgag
     4321 tgagttgtgg tgacagttta acctctctac atttgtaata ataagcattg ttttggcgta
     4381 tcaatagtct gtagattttt tcacaaagaa acctggagaa atgtacacaa atatctaaat
     4441 gaaaattggc gttacgtaac aaataccaac gccccgaaaa tccgtatata tgcaaagcat
     4501 tctgatatat atatatataa gtggtataca tatacatatt cgtatcggat ccgtgatcta
     4561 tatttaggaa ccctcgaccg ccggagtata aatagaggcg cttcgtctac ggagcgacaa
     4621 ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc
     4681 gcagctgaac aagctaaaca atcggggtac cgctagagtc gacggtaccg cgggccgcgg
     4741 ctcgagggta ccaatcaaac atgtccaaag gtgaagaact gtttaccgga gtagtcccga
     4801 tattggttga actcgacggc gatgtcaacg gtcataaatt cagtgtgtcc ggcgagggtg
     4861 agggcgacgc cacatacggt aagctgacgt tgaagttcat atgcaccacg ggcaagctgc
     4921 ccgtgccatg gccgacgttg gtcacgacgc tgacgtatgg tgtccagtgt ttcagccgtt
     4981 accccgatca tatgaagcag catgactttt tcaagtcggc gatgccggag ggatacgttc
     5041 aagagaggac cattttcttc aaggatgacg gcaactataa gacgcgagcg gaggtgaaat
     5101 ttgaaggcga cacactcgtt aaccgtattg agttgaaggg cattgatttt aaggaggatg
     5161 gcaacattct gggccataag ttggaataca actacaactc gcataatgtg tatataatgg
     5221 cagataagca gaaaaacgga ataaaggtta acttcaagat tcgccacaac atagaggacg
     5281 gttccgtgca acttgcagat cattaccaac agaacacacc cattggagat ggcccagttc
     5341 tcttgccaga caatcactac ctttccacac agtccgcgtt gagcaaggac cccaatgaaa
     5401 agcgggacca catggtgttg ctggagtttg tgaccgcagc tggtattaca cacggcatgg
     5461 atgagctcta caagtaatct agaggatctt tgtgaaggaa ccttacttct gtggtgtgac
     5521 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     5581 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
     5641 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
     5701 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
     5761 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
     5821 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
     5881 agctgcactg ctatacaaga aaattatgga aaaatatttg atgtatagtg ccttgactag
     5941 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     6001 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     6061 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6121 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     6181 tcgatggcgc gcctccggaa cataatggtg catctagatg cattcgcgag gtaccgagct
     6241 cggagctgga caactttgta tagaaaagtt gaacgagaaa cgtaaaatga tataaatatc
     6301 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
     6361 atccagtcac tatgcgacgt gcagactggc tgtgtataag ggagcctgac attatattcc
     6421 ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac
     6481 ttcttccccg ataacggaaa ccggcacact ggccatatcg gtggtcatca tgcgccagct
     6541 ttcatccccg atatgcacca ccgggtaaag ttcacgggag actttatctg agagcaagcg
     6601 cgcagacgca agtggaataa ccatgcgacg gcccggtgta tcgataatat cactctgtac
     6661 atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc
     6721 accagcccct gttctcgtca gcaaaagagc cgttcatttc aataaaccgg gcgacctcag
     6781 ccatcccttc ctgattttcc gctttccagc gttcggcacg cagacgacgg gcttcattct
     6841 gcatggttgt gcttaccaga ccggagatat tgacatcata tatgccttga gcaactgata
     6901 gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata gcatacctct ttttgacata
     6961 cttcgggtat acatatcagt atatattctt ataccgcaaa aatcagcgcg caaatacgca
     7021 tactgttatc tggcttttag taagccggat ccattaccaa tgcttaatca gtgaggcacc
     7081 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7141 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgggaccc
     7201 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7261 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7321 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7381 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7441 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7501 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7561 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7621 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7681 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7741 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7801 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7861 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7921 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7981 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgt agctcctgaa aatctcgacg
     8041 gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta aagcctgggg
     8101 tgcctaatgc ggcccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
     8161 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa
     8221 ctttattata caaagttgag gccccgacgt taaattgaaa ataggtctat atatacgaac
     8281 tgagtctgga aaaagaagtt gagaattata aaaagtagtg agcactggcg ccctctctgc
     8341 ttggcgagct aaccttttcg cctcttggct gagtaggtgg cgtttcattc tactctgtaa
     8401 aattaatgta gaattgaaac actatggtca aaaaatactt aggggcataa gttataaaac
     8461 gtatgaaatg aatttttatc aacctgggct attcaaaaat tttcgaatta ttttatgtat
     8521 tttttttaat cgtttttcat tataggttaa aatacacttt aaaaggaatt ctttcctgta
     8581 aaataaatat aaataaatat gctttattga cagaaaattt gatgtttttg tatttgagta
     8641 ggagcaatca caggtgagca aaaaacccat atgttcggct tgtcgatcgg atcccctccg
     8701 taccaagact tcgaattcgg atccctcgag cgatacacac ttctatagtg tcacctaaat
     8761 gcgtttaaac cttcctgcag gtgacgattt cctcgctcac tgactcgctg cgctcggtcg
     8821 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     8881 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     8941 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9001 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9061 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9121 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9181 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9241 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9301 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9361 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     9421 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9481 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9541 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9601 actcacgtta agggattttg gtcatgcatg atatatctcc caatttgtgt agggcttatt
     9661 atgcacgctt aaaaataata aaagcagact tgacctgata gtttggctgt gagcaattat
     9721 gtgcttagtg catctaacgc ttgagttaag ccgcgccgcg aagcggcgtc ggcttgaacg
     9781 aatttctagc tagacattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga
     9841 caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag
     9901 cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt
     9961 cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca
    10021 acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta
    10081 aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg
    10141 ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat
    10201 caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc
    10261 caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa
    10321 tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa
    10381 ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca
    10441 aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg
    10501 ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg
    10561 atacttcggc gatcaccgct tcccccatga tgtttaactt tgttttaggg cgactgccct
    10621 gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt
    10681 gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acagtcataa caagccatga
    10741 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
    10801 agcgcatacg ctacttgcat tacagcttac gaaccgaaca ggcttatgtc cactgggttc
    10861 gtgcccgaat tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
    10921 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
    10981 ggtaacatga gcaaagtc
//
