LOCUS       pJAT31{dU6-3-attR3-Amp-ccdB-attR4-attP-B3-ie1-DsRed-B3-attR5-Chlor-SacB-attR6-dU6-1}       11224 bp    DNA     circular UNA 12-JUN-2023
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local.sternd.1pf-gdrcxto
VERSION     urn.local.sternd.1pf-gdrcxto
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             411..1202
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /standard_name="SmR"
     rep_origin      1448..2036
     misc_feature    1820..1837
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ori-252 R"
     primer_bind     1937..1956
                     /note="pBR322 origin, forward primer"
                     /standard_name="pBR322ori-F"
     primer_bind     2026..2045
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7Cas9F"
     primer_bind     complement(2052..2071)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp-ori-7571F"
     misc_feature    2180..2209
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     2190..2207
                     /note="L4440 vector, forward primer"
                     /standard_name="L4440"
     primer_bind     2210..2229
                     /standard_name="pGGAselect PCR Product (1 F) - pGWB402
                     (digested by PsyI, SapI) Fragment 4"
     regulatory      2239..2258
                     /note="Geneious type: promoter"
                     /note="SP6 RNA polymerase promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SP6"
     misc_feature    2300..2303
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2304..2333
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     primer_bind     2304..2322
                     /standard_name="1 F"
     regulatory      2334..2754
                     /note="Geneious type: promoter"
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
                     /standard_name="dU6-3 promoter"
     misc_feature    complement(2399..2409)
                     /note="Geneious type: Transcription Factor"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Hb"
     misc_feature    2755..2787
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    2758..2787
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2761..2884
                     /note="Geneious type: attR3"
                     /standard_name="attR3"
     regulatory      2907..2937
                     /note="Geneious type: promoter"
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacUV5"
     misc_feature    complement(2918..2929)
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     primer_bind     complement(2958..2979)
                     /standard_name="676 R"
     misc_feature    2976..2979
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      2980..3084
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     primer_bind     2980..2999
                     /standard_name="1 F"
     misc_feature    3085..3945
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     primer_bind     complement(3125..3146)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AMP_5'_F"
     misc_feature    3331..3350
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_R"
     misc_feature    3804
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="T"
     primer_bind     complement(3817..3836)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_F"
     misc_feature    3909..3932
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp_3'_R"
     primer_bind     complement(3922..3945)
                     /standard_name="966 R"
     misc_feature    3942..3945
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    3946..4585
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGT
                     TGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAAT
                     GAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
                     AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTG
                     AAATGCAATTTAAGGTTTATACTTACAAGCGCGAGTCCCGCTATCGCCTTTTCGTGGA
                     CGTCCAGTCAGACATTATCGACACGCCTGGTCGCCGTATGGTGATTCCATTAGCCAGC
                     GCACGCCTGTTAAGTGATAAGGTGTCGCGCGAGCTTTACCCAGTCGTCCATATTGGTG
                     ACGAGAGTTGGCGCATGATGACTACAGACATGGCTAGTGTACCTGTGTCGGTGATCGG
                     GGAAGAGGTCGCGGATTTAAGCCACCGTGAGAATGATATTAAGAATGCAATCAATCTC
                     ATGTTTTGGGGTATTTA"
     primer_bind     3946..3967
                     /standard_name="1 F"
     gene            4255..4587
                     /standard_name="ccdB"
     primer_bind     4446..4465
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="4446F"
     misc_feature    complement(4625..4748)
                     /note="Geneious type: attR4"
                     /standard_name="attR4"
     misc_feature    4752..4755
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    4758..4976
                     /note="Geneious type: attP"
                     /note="Derived using Geneious Prime 2020.2.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="attP"
     misc_feature    4829..4912
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="core attP"
     misc_feature    complement(4874..4889)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="inverted repeat2"
     misc_feature    4977..5009
                     /note="Geneious type: Recombination site"
                     /standard_name="B3"
     misc_feature    4999..5028
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    5010..5029
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="1,685 R"
     primer_bind     5010..5029
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="DSHR840R"
     misc_feature    5029..6128
                     /locus_tag="ie1 promoter"
                     /note="Geneious type: Enhancer"
                     /standard_name="ie1"
     primer_bind     5029..5045
                     /standard_name="ie1-prom (1 F) - P777
                     p{dU6-3-GW1-Amp-ccdB-attP-B3-3xP3::dsRed-B3-GW2-Chlor-SacB
                     (digested by BseRI, SphI) Fragment 2"
     regulatory      5046..5528
                     /note="Geneious type: enhancer"
                     /note="Autographa californica hr5 enhancer"
                     /regulatory_class="enhancer"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="hr5"
     regulatory      5532..6123
                     /note="Geneious type: promoter"
                     /note="Autographa californica nucleopolyhedrovirus IE-1
                     promoter region"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="IE-1"
     misc_feature    <6129..6131
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="ATGCAATGGTTAATTCGAGCTCGCCCGGGGATCTAATTCAAT
                     TAGAGACTAATTCAATTAGAGCTAATTCAATTAGGATCCAAGCTTATCGATTTCG"
     misc_feature    6129..6161
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    <6136..>6309
                     /note="Geneious type: synthetic DNA"
                     /standard_name="mTurq cassette"
     misc_feature    complement(6136..6155)
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="4558 R"
     regulatory      6142..6309
                     /note="Geneious type: promoter"
                     /standard_name="HS promoter"
     misc_feature    complement(6180..6198)
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="12878 R"
     misc_feature    complement(6180..6198)
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2020.2.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="P693
                     p(MHC-DsRed-CDNopt-5'myr-smGFP-int-B3-B3 recombinase-FSF)
                     - 12878 R (reversed)"
     primer_bind     complement(6292..6309)
                     /standard_name="ie1-prom (1281 R) - P777
                     p{dU6-3-GW1-Amp-ccdB-attP-B3-3xP3::dsRed-B3-GW2-Chlor-SacB
                     (digested by BseRI, SphI) Fragment 2"
     regulatory      <6310..6311
                     /note="Geneious type: Promoter"
                     /standard_name="HS prom"
     misc_feature    6310..6339
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    6312..6991
                     /note="Geneious type: reporter"
                     /standard_name="DsRed2 reporter"
     primer_bind     complement(6438..6457)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="DsRed-3573R"
     misc_feature    6523..6526
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      6993..7237
                     /note="Geneious type: terminator"
                     /standard_name="SV40_PA term"
     misc_feature    complement(7040..7059)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Binding Region"
     misc_feature    complement(7040..7059)
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40-PA-R"
     misc_feature    7042..7074
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      7100..7221
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     primer_bind     7127..7146
                     /standard_name="Binding Region"
     primer_bind     7127..7146
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40-PA-F"
     primer_bind     7175..7195
                     /standard_name="Binding Region"
     primer_bind     7175..7195
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="1198F"
     primer_bind     7242..7261
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="8,388 F"
     primer_bind     7270..7289
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="2655R"
     primer_bind     complement(7285..7304)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="dsRed-391F"
     misc_feature    7305..7337
                     /note="Geneious type: Recombination site"
                     /standard_name="B3"
     misc_feature    7338..7461
                     /note="Geneious type: attR5"
                     /standard_name="attR5"
     misc_feature    7338..7341
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      7484..7514
                     /note="Geneious type: promoter"
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacUV5"
     misc_feature    complement(7495..7506)
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     gene            7568..8227
                     /gene="CAT/CamR"
                     /note="Geneious type: CDS"
                     /standard_name="CAT/CamR"
     misc_feature    7573..8225
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AAAAAAGATTACGGGTTATACTACCGTCGACATCAGCCAGTG
                     GCACCGTAAAGAACATTTCGAAGCGTTCCAGAGCGTCGCGCAGTGCACCTACAATCAG
                     ACCGTTCAGCTGGATATCACTGCTTTCCTGAAGACTGTTAAGAAAAACAAACACAAAT
                     TTTATCCGGCTTTTATCCACATTCTCGCTCGCCTGATGAACGCACACCCGGAATTCCG
                     TATGGCCATGAAAGATGGCGAGCTGGTAATTTGGGATTCCGTTCATCCGTGTTATACC
                     GTCTTTCACGAACAGACCGAGACGTTCTCTTCCCTGTGGTCTG"
     primer_bind     complement(7666..7685)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="10,588 R"
     primer_bind     complement(8219..8235)
                     /standard_name="905 R"
     misc_feature    8230..10102
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CGAGACGTTCTCTTCCCTGTGGTCTGAATATCACGATGACTT
                     CCGTCAATTCCTGCACATTTATTCTCAGGACGTTGCGTGCTATGGTGAGAATCTCGCT
                     TATTTTCCGAAGGGTTTCATTGAAAACATGTTCTTTGTTAGCGCCAACCCATGGGTGA
                     GCTTCACTTCTTTCGACCTGAACGTGGCGAACATGGACAACTTTTTCGCGCCGGTTTT
                     CACGATGGGCAAGTATTACACCCAGGGTGACAAGGTACTGATGCCGCTGGCAATCCAG
                     GTGCATCACGCGGTATGTGACGGCTTCCATGTCGGTCGCATGCTGAACGAGCTGCAGC
                     AATACTGTGATGAATGGCAGGGCGGTGCGTAAACGCGTGGATCCGGCTTACTAAAAGC
                     CAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGAT
                     ATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGAC
                     AGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTC
                     TGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGC
                     GGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTT
                     GCTGACGAGAACAGGGGCTGGTGAAATGCAATTTAAAGTTTATACCTACAAGCGCGAA
                     TCTCGCTACCGTCTGTTTGTAGACGTACAGTCTGATATCATTGATACGCCGGGCCGCC
                     GTATGGTTATTCCGCTGGCATCTGCACGTCTCCTGTCTGATAAAGTTAGCCGTGAACT
                     GTATCCGGTTGTACACATTGGCGACGAGTCTTGGCGCATGATGACGACTGACATGGCT
                     TCTGTCCCGGTTAGCGTCATTGGCGAGGAAGTCGCCGATCTGTCTCACCGTGAAAATG
                     ACATCAAAAATGCAATTAACCTGATGTTCTGGGGCATTTGATCCGGCTTACTAAAAGC
                     CAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGAT
                     ATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGAC
                     AGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTC
                     TGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGC
                     GGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTT
                     GCTGACGAGAACAGGGGCTGGTGAAATGCAATTTAAAGTTTATACCTACAAGCGCGAA
                     TCTCGCTACCGTCTGTTTGTAGACGTACAGTCTGATATCATTGATACGCCGGGCCGCC
                     GTATGGTTATTCCGCTGGCATCTGCACGTCTCCTGTCTGATAAAGTTAGCCGTGAACT
                     GTATCCGGTTGTACACATTGGCGACGAGTCTTGGCGCATGATGACGACTGACATGGCT
                     TCTGTCCCGGTTAGCGTCATTGGCGAGGAAGTCGCCGATCTGTCTCACCGTGAAAATG
                     ACATCAAAAATGCAATTAACCTGATGTTCTGGGGCATTTG"
     misc_feature    8232..8235
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      8236..8681
                     /note="Geneious type: promoter"
                     /note="Bacillus subtilis sacB (glycoside hydrolase family
                     68) promoter region"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB"
     primer_bind     8236..8256
                     /standard_name="1 F"
     gene            8682..10106
                     /note="counterselectable marker from Bacillus subtilis
                     that converts sucrose to levans"
                     /product="levansucrase"
                     /gene="sacB"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB"
     primer_bind     complement(9056..9076)
                     /standard_name="841 R"
     misc_feature    9073..9076
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     9077..9099
                     /standard_name="1 F"
     misc_feature    complement(10142..10265)
                     /note="Geneious type: attR6"
                     /standard_name="attR6"
     misc_feature    10266..10295
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     primer_bind     complement(10271..10295)
                     /standard_name="1,219 R"
     misc_feature    10288..10317
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="GTCTTCTCGAAGACCC"
     misc_feature    10288..10317
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    10296..10299
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      complement(10318..11121)
                     /note="Geneious type: promoter"
                     /standard_name="U6:1 promoter"
     primer_bind     10769..10789
                     /standard_name="attB F seq"
     misc_feature    10983
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     misc_feature    11001
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     misc_feature    11010
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     misc_feature    11020
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     misc_feature    11058
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="G"
     misc_feature    11122..11151
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    11122..11151
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      complement(11164..11182)
                     /note="Geneious type: Promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7 prom"
     primer_bind     complement(11175..11194)
                     /standard_name="pGGAselect PCR Product (270 R) - pGWB402
                     (digested by PsyI, SapI) Fragment 4"
     misc_feature    11195..11224
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
ORIGIN      
        1 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
       61 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
      121 tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
      181 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
      241 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
      301 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      361 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
      421 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
      481 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
      541 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
      601 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
      661 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
      721 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
      781 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
      841 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
      901 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
      961 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     1021 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1081 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     1141 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     1201 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     1261 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     1321 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca
     1381 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1441 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1501 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1561 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1621 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1681 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1741 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1801 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1861 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1921 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1981 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2041 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2101 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2161 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa atcgtcacct
     2221 gcaggaaggt ttaaacgcat ttaggtgaca ctatagaagt gtgtatcgct cgagggatcc
     2281 gaattcgaag tcttggtacg gaggggatcc gatcgacaag ccgaacatat gggttttttg
     2341 ctcacctgtg attgctccta ctcaaataca aaaacatcaa attttctgtc aataaagcat
     2401 atttatttat atttatttta caggaaagaa ttccttttaa agtgtatttt aacctataat
     2461 gaaaaacgat taaaaaaaat acataaaata attcgaaaat ttttgaatag cccaggttga
     2521 taaaaattca tttcatacgt tttataactt atgcccctaa gtattttttg accatagtgt
     2581 ttcaattcta cattaatttt acagagtaga atgaaacgcc acctactcag ccaagaggcg
     2641 aaaaggttag ctcgccaagc agagagggcg ccagtgctca ctacttttta taattctcaa
     2701 cttctttttc cagactcagt tcgtatatat agacctattt tcaatttaac gtcggggcct
     2761 caactttgta taataaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     2821 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     2881 tatgggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
     2941 ggattttgag ttaggatccg tcgagatttt caggagctac gcggaacccc tatttgttta
     3001 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3061 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3121 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3181 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3241 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3301 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3361 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3421 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3481 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3541 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3601 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3661 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3721 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3781 tctggagccg gtgagcgtgg gtcccgcggt atcattgcag cactggggcc agatggtaag
     3841 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3901 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaatggat ccggcttact
     3961 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     4021 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     4081 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     4141 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     4201 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     4261 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     4321 tatcgtctgt ttgtggatgt acagagtgat attatcgata caccgggccg tcgcatggtt
     4381 attccacttg cgtctgcgcg cttgctctca gataaagtct cccgtgaact ttacccggtg
     4441 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtt
     4501 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     4561 attaacctga tgttctgggg aatataatgt caggctccct tatacacagc cagtctgcac
     4621 gtcgcatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     4681 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa cttttctata
     4741 caaagttgtc cagctgtact gacggacaca ccgaagcccc ggcggcaacc ctcagcggat
     4801 gccccggggc ttcacgtttt cccaggtcag aagcggtttt cgggagtagt gccccaactg
     4861 gggtaacctt tgagttctct cagttggggg cgtagggtcg ccgacatgac acaaggggtt
     4921 gtgaccgggg tggacacgta cgcgggtgct tacgaccgtc agtcgcgcga gcgcgaggtt
     4981 gcttaagaat aagtaattct taagcaacca ttacgccaag cttgcatgcc gagctcttac
     5041 gcgcccgcgt aaaacacaat caagtatgag tcataagctg atgtcatgtt ttgcacacgg
     5101 ctcataaccg aactggcttt acgagtagaa ttctacttgt aacgcacgat cagtggatga
     5161 tgtcatttgt ttttcaaatc gagatgatgt catgttttgc acacggctca taaactcgct
     5221 ttacgagtag aattctacgt gtaacgcacg atcgattgat gagtcatttg ttttgcaata
     5281 tgatatcata caatatgact catttgtttt tcaaaaccga acttgattta cgggtagaat
     5341 tctacttgta aagcacaatc aaaaagatga tgtcatttgt ttttcaaaac tgaactcgct
     5401 ttacgagtag aattctacgt gtaaaacaca atcaagaaat gatgtcattt gttataaaaa
     5461 taaaagctga tgtcatgttt tgcacatggc tcataactaa actcgcttta cgggtagaat
     5521 tctacgcgcg tcgatgtctt tgtgatgcgc gcgacatttt tgtaggttat tgataaaatg
     5581 aacggatacg ttgcccgaca ttatcattaa atccttggcg tagaatttgt cgggtccatt
     5641 gtccgtgtgc gctagcatgc ccgtaacgga cctcgtactt ttggcttcaa aggttttgcg
     5701 cacagacaaa atgtgccaca cttgcagctc tgcatgtgtg cgcgttacca caaatcccaa
     5761 cggcgcagtg tacttgttgt atgcaaataa atctcgataa aggcgcggcg cgcgaatgca
     5821 gctgatcacg tacgctcctc gtgttccgtt caaggacggt gttatcgacc tcagattaat
     5881 gtttatcggc cgactgtttt cgtatccgct caccaaacgc gtttttgcat taacattgta
     5941 tgtcggcgga tgttctatat ctaatttgaa taaataaacg ataaccgcgt tggttttaga
     6001 gggcataata aaagaaatat tgttatcgtg ttcgccatta gggcagtata aattgacgtt
     6061 catgttggat attgtttcag ttgcaagttg acactggcgg cgacaagatc gtgaacaacc
     6121 aagtgaccat taaccctcga ccgccggagt ataaatagag gcgcttcgtc tacggagcga
     6181 caattcaatt caaacaagca aagtgaacac gtcgctaagc gaaagctaag caaataaaca
     6241 agcgcagctg aacaagctaa acaatcgggg taccgctaga gtcgacggta cgatccaccg
     6301 gtcgccaccc catggtgcgc tcctccaaga acgtcatcaa ggagttcatg cgcttcaagg
     6361 tgcgcatgga gggcaccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     6421 gcccctacga gggccacaac accgtgaagc tgaaggtgac caagggcggc cccctgccct
     6481 tcgcctggga catcctgtcc ccccagttcc agtacggctc caaggtgtac gtgaagcacc
     6541 ccgccgacat ccccgactac aagaagctgt ccttccccga gggcttcaag tgggagcgcg
     6601 tgatgaattt cgaagatggt ggagttgtca cagtcacaca agattcttcg ctccaagatg
     6661 gttgttttat ttacaaagtg aaatttattg gagtcaattt tccgtcggat ggaccggtta
     6721 tgcaaaagaa aacaatgggt tgggaagcat cgacggaacg tctgtatcca cgagatggag
     6781 ttttgaaagg cgaaattcac aaagctctta aactcaaaga tggcggacat taccttgtag
     6841 aatttaaaag catttatatg gctaagaaac cggtccaact ccctggatat tattatgtcg
     6901 attcgaaact ggatattact tcacataacg aagattatac gatagttgag caatatgaac
     6961 gcactgaagg tcgtcaccat ctctttctct aaagatcata atcagccata ccacatttgt
     7021 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     7081 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     7141 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7201 caaactcatc aatgtatctt atcatgtctg gatcgatggc gcgcctccgg aacataatgg
     7261 tgcatctaga tgcattcgcg aggtaccgag ctcgaattca ctggggttgc ttaagaataa
     7321 gtaattctta agcaacccaa ctttgtatac aaaagttgaa cgagaaacgt aaaatgatat
     7381 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     7441 acaacatatc cagtcactat gggccgcatt aggcacccca ggctttacac tttatgcttc
     7501 cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga
     7561 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
     7621 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
     7681 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
     7741 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
     7801 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
     7861 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
     7921 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
     7981 tattgagaat atgtttttcg tgtcagccaa tccctgggtg agtttcacca gttttgattt
     8041 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
     8101 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg
     8161 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
     8221 ggcgtaaacg cgtggcacat atacctgccg ttcactatta tttagtgaaa tgagatatta
     8281 tgatattttc tgaattgtga ttaaaaaggc aactttatgc ccatgcaaca gaaactataa
     8341 aaaatacaga gaatgaaaag aaacagatag attttttagt tctttaggcc cgtagtctgc
     8401 aaatcctttt atgattttct atcaaacaaa agaggaaaat agaccagttg caatccaaac
     8461 gagagtctaa tagaatgagg tcgaaaagta aatcgcgcgg gtttgttact gataaagcag
     8521 gcaagaccta aaatgtgtaa agggcaaagt gtatactttg gcgtcacccc ttacatattt
     8581 taggtctttt tttattgtgc gtaactaact tgccatcttc aaacaggagg gctggaagaa
     8641 gcagaccgct aacacagtac ataaaaaagg agacatgaac gatgaacatc aaaaagtttg
     8701 caaaacaagc aacagtatta acctttacta ccgcactgct ggcaggaggc gcaactcaag
     8761 cgtttgcgaa agaaacgaac caaaagccat ataaggaaac atacggcatt tcccatatta
     8821 cacgccatga tatgctgcaa atccctgaac agcaaaaaaa tgaaaaatat caagttcctg
     8881 aattcgattc gtccacaatt aaaaatatct cttctgcaaa aggcctggac gtttgggaca
     8941 gctggccatt acaaaacgct gacggcactg tcgcaaacta tcacggctac cacatcgtct
     9001 ttgcattagc cggagatcct aaaaatgcgg atgacacatc gatttacatg ttctatcaaa
     9061 aagtcggcga aacttctatt gacagctgga aaaacgctgg ccgcgtcttt aaagacagcg
     9121 acaaattcga tgcaaatgat tctatcctaa aagaccaaac acaagaatgg tcaggttcag
     9181 ccacatttac atctgacgga aaaatccgtt tattctacac tgatttctcc ggtaaacatt
     9241 acggcaaaca aacactgaca actgcacaag ttaacgtatc agcatcagac agctctttga
     9301 acatcaacgg tgtagaggat tataaatcaa tctttgacgg tgacggaaaa acgtatcaaa
     9361 atgtacagca gttcatcgat gaaggcaact acagctcagg cgacaaccat acgctgagag
     9421 atcctcacta cgtagaagat aaaggccaca aatacttagt atttgaagca aacactggaa
     9481 ctgaagatgg ctaccaaggc gaagaatctt tatttaacaa agcatactat ggcaaaagca
     9541 catcattctt ccgtcaagaa agtcaaaaac ttctgcaaag cgataaaaaa cgcacggctg
     9601 agttagcaaa cggcgctctc ggtatgattg agctaaacga tgattacaca ctgaaaaaag
     9661 tgatgaaacc gctgattgca tctaacacag taacagatga aattgaacgc gcgaacgtct
     9721 ttaaaatgaa cggcaaatgg tacctgttca ctgactcccg cggatcaaaa atgacgattg
     9781 acggcattac gtctaacgat atttacatgc ttggttatgt ttctaattct ttaactggcc
     9841 catacaagcc gctgaacaaa actggccttg tgttaaaaat ggatcttgat cctaacgatg
     9901 taacctttac ttactcacac ttcgctgtac ctcaagcgaa aggaaacaat gtcgtgatta
     9961 caagctatat gacaaacaga ggattctacg cagacaaaca atcaacgttt gcgccaagct
    10021 tcctgctgaa catcaaaggc aagaaaacat ctgttgtcaa agacagcatc cttgaacaag
    10081 gacaattaac agttaacaaa taaatgtcag gctcccttat acacagccag tctgcacgtc
    10141 gcatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
    10201 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt tttaatacaa
    10261 agttgcctcc ggaacataat ggtgcatcta gatgcccatt cctgtagact tcttaatcga
    10321 agttcacccg gatatctttc ctatatatac tgtactcttg cagaatatgt acttgggaat
    10381 cataaggatg cgaaggacgt acgcgctgtg tctgtaacta aggggttgct ttcgggtacc
    10441 taccatgcca atgatgggac taccgccgga taggaatccc ttttgggtgc acaccgtcac
    10501 ctccagttcc gtctgcctga gttcggcaat ggaaatcagg tactcaaagg atgcatcgta
    10561 gacggggttg cagttgtcct tgatcacgct cgtcttgcgt ttcgactcct tggtgcgtcc
    10621 aggcaacaga tacagcttaa catacggatc ggggatattg ctgggatcgc gaagtggtat
    10681 cttctggatt ttgtgtatgg tcacgtctag tttttgacgc tgggcgctgt agcggatcga
    10741 caactgcatt cgtccaaggc catgttcgcc gggtgaggag gtggagtctg gaaagcggtg
    10801 ggtcagcaca gaggtggcca gctcgctgat gggtttttcg ctggaggtgg aggccgacat
    10861 ggccggggag attttcgtag ccgccacagg atcttcttca ctgatgggcg attcggcgga
    10921 caaacgcttg ctggatgcct gagattccta aaagcaatgg caaattagat tcaattaaaa
    10981 cagaaattgt ttatataaca gagtaaaagc aaaaaaaaag gaatcacaga ttctccccga
    11041 cggggaattg aaccccgttc tcccgcgtga caggcgggga tactaaccac tatactatcg
    11101 aggacgttga aaatgaattc aattaagacg tcagaattct cgaggcggcc gcatgtgagt
    11161 ctccctatag tgagtcgtat taatttcgcg ggcgcgtcag cgggagagcc gttgtaaggc
    11221 ggca
//
