LOCUS       pJAT7{dU6-3-attR3-Amp-ccdB-attR4-attP-B3-3XP3-DsRed-B3-attR5-Chlor-SacB-attR6}        9454 bp    DNA     circular UNA 12-JUN-2023
DEFINITION  Golden Gate/Type IIS Cloning ligation product.
ACCESSION   urn.local.sternd.1pv-gdrd0dr
VERSION     urn.local.sternd.1pv-gdrd0dr
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             411..1202
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /standard_name="SmR"
     rep_origin      1448..2036
     misc_feature    1820..1837
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ori-252 R"
     primer_bind     1937..1956
                     /note="pBR322 origin, forward primer"
                     /standard_name="pBR322ori-F"
     primer_bind     2026..2045
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7Cas9F"
     primer_bind     complement(2052..2071)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp-ori-7571F"
     misc_feature    2180..2209
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     2190..2207
                     /note="L4440 vector, forward primer"
                     /standard_name="L4440"
     primer_bind     2210..2229
                     /standard_name="pGGAselect PCR Product (1 F) - pGWB402
                     (digested by PsyI, SapI) Fragment 4"
     regulatory      2239..2258
                     /note="Geneious type: promoter"
                     /note="SP6 RNA polymerase promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SP6"
     misc_feature    2300..2303
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2304..2333
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     primer_bind     2304..2322
                     /standard_name="1 F"
     regulatory      2334..2754
                     /note="Geneious type: promoter"
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
                     /standard_name="dU6-3 promoter"
     misc_feature    2755..2787
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    2758..2787
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2761..2884
                     /note="Geneious type: attR3"
                     /standard_name="attR3"
     primer_bind     complement(2958..2979)
                     /standard_name="676 R"
     misc_feature    2976..2979
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      2980..3084
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     primer_bind     2980..2999
                     /standard_name="1 F"
     misc_feature    3085..3945
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     primer_bind     complement(3125..3146)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AMP_5'_F"
     misc_feature    3331..3350
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_R"
     misc_feature    3804
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="T"
     primer_bind     complement(3817..3836)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_F"
     misc_feature    3909..3932
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp_3'_R"
     primer_bind     complement(3922..3945)
                     /standard_name="966 R"
     misc_feature    3942..3945
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    3946..4585
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGT
                     TGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAAT
                     GAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
                     AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTG
                     AAATGCAATTTAAGGTTTATACTTACAAGCGCGAGTCCCGCTATCGCCTTTTCGTGGA
                     CGTCCAGTCAGACATTATCGACACGCCTGGTCGCCGTATGGTGATTCCATTAGCCAGC
                     GCACGCCTGTTAAGTGATAAGGTGTCGCGCGAGCTTTACCCAGTCGTCCATATTGGTG
                     ACGAGAGTTGGCGCATGATGACTACAGACATGGCTAGTGTACCTGTGTCGGTGATCGG
                     GGAAGAGGTCGCGGATTTAAGCCACCGTGAGAATGATATTAAGAATGCAATCAATCTC
                     ATGTTTTGGGGTATTTA"
     primer_bind     3946..3967
                     /standard_name="1 F"
     gene            4255..4587
                     /standard_name="ccdB"
     misc_feature    complement(4625..4748)
                     /note="Geneious type: attR4"
                     /standard_name="attR4"
     misc_feature    4752..5562
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="attP-B3-3xP3::dsRed-B3 part 1
                     (concatenated sequence 2)"
     misc_feature    4752..4755
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    4758..4976
                     /note="Geneious type: attP"
                     /note="Derived using Geneious Prime 2020.2.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="attP"
     misc_feature    <4758..4976
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="attP (concatenated sequence 2)"
     misc_feature    4977..5009
                     /note="Geneious type: Recombination site"
                     /standard_name="B3"
     misc_feature    <4977..>5009
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pUC57{Mhc-678::dsRed} (digested by NdeI,
                     AscI) Fragment 2 - attB-T2A-Bpu10I-hsp70 1 -
                     attB-T2A-Bpu10I-hsp70 2 (digested by Bpu10I) (concatenated
                     sequence 1)"
     misc_feature    4977..5009
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="B3 (concatenated sequence 3)"
     misc_feature    5010..5029
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="1685R"
     misc_feature    5010..>5562
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="3xP3::dsRed (concatenated sequence 4)"
     primer_bind     5010..5029
                     /standard_name="Binding Region"
     misc_feature    5094..5106
                     /note="Geneious type: dna binding region"
                     /standard_name="P3"
     misc_feature    5110..5122
                     /note="Geneious type: dna binding region"
                     /standard_name="P3"
     misc_feature    5125..5137
                     /note="Geneious type: dna binding region"
                     /standard_name="P3"
     misc_feature    complement(5163..5182)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Binding Region"
     regulatory      5169..5347
                     /note="Geneious type: Promoter"
                     /standard_name="HS prom"
     misc_feature    5348..6027
                     /note="Geneious type: reporter"
                     /standard_name="DsRed2 reporter"
     misc_feature    <5559..6028
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="GTACGTGAAGCACCCCGCCGACATCCCCGACTACAAGAAGCT
                     GTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTG
                     GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCTGCTTCATCTACAAGGTGAAGT
                     TCATCGGCGTGAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTG
                     GGAGGCCTCCACCGAGCGCCTGTACCCCCGCGACGGCGTGCTGAAGGGCGAGATCCAC
                     AAGGCCCTGAAGCTGAAGGACGGCGGCCACTACCTGGTGGAGTTCAAGTCCATCTACA
                     TGGCCAAGAAGCCCGTGCAGCTGCCCGGCTACTACTACGTGGACTCCAAGCTGGACAT
                     CACCTCCCACAACGAGGACTACACCATCGTGGAGCAGTACGAGCGCACCGAGGGCCGC
                     CACCACCTGTTCCTGTAG"
     misc_feature    5559..6377
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="attP-B3-3xP3::dsRed-B3 part 2
                     (concatenated sequence 3)"
     misc_feature    5559..5562
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      6029..6273
                     /note="Geneious type: terminator"
                     /standard_name="SV40_PA term"
     misc_feature    <6029..6340
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="3xP3::dsRed (concatenated sequence 4)"
     misc_feature    complement(6076..6095)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Binding Region"
     primer_bind     6163..6182
                     /standard_name="Binding Region"
     primer_bind     6211..6231
                     /standard_name="Binding Region"
     misc_feature    6341..6373
                     /note="Geneious type: Recombination site"
                     /standard_name="B3"
     misc_feature    <6341..>6373
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pUC57{Mhc-678::dsRed} (digested by NdeI,
                     AscI) Fragment 2 - attB-T2A-Bpu10I-hsp70 1 -
                     attB-T2A-Bpu10I-hsp70 2 (digested by Bpu10I) (concatenated
                     sequence 1)"
     misc_feature    6341..6373
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="B3 Copy (concatenated sequence 5)"
     misc_feature    6374..6497
                     /note="Geneious type: attR5"
                     /standard_name="attR5"
     misc_feature    6374..4755
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P767
                     p{dU6-3-GW1-Amp-ccdB-Mhc-GFP-FRT-P3-DsRed-GW2-Chlor-SacB}
                     (concatenated sequence 1)"
     misc_feature    6374..6377
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     gene            6604..7263
                     /gene="CAT/CamR"
                     /note="Geneious type: CDS"
                     /standard_name="CAT/CamR"
     misc_feature    6609..7261
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AAAAAAGATTACGGGTTATACTACCGTCGACATCAGCCAGTG
                     GCACCGTAAAGAACATTTCGAAGCGTTCCAGAGCGTCGCGCAGTGCACCTACAATCAG
                     ACCGTTCAGCTGGATATCACTGCTTTCCTGAAGACTGTTAAGAAAAACAAACACAAAT
                     TTTATCCGGCTTTTATCCACATTCTCGCTCGCCTGATGAACGCACACCCGGAATTCCG
                     TATGGCCATGAAAGATGGCGAGCTGGTAATTTGGGATTCCGTTCATCCGTGTTATACC
                     GTCTTTCACGAACAGACCGAGACGTTCTCTTCCCTGTGGTCTG"
     primer_bind     complement(7255..7271)
                     /standard_name="905 R"
     misc_feature    7266..9138
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CGAGACGTTCTCTTCCCTGTGGTCTGAATATCACGATGACTT
                     CCGTCAATTCCTGCACATTTATTCTCAGGACGTTGCGTGCTATGGTGAGAATCTCGCT
                     TATTTTCCGAAGGGTTTCATTGAAAACATGTTCTTTGTTAGCGCCAACCCATGGGTGA
                     GCTTCACTTCTTTCGACCTGAACGTGGCGAACATGGACAACTTTTTCGCGCCGGTTTT
                     CACGATGGGCAAGTATTACACCCAGGGTGACAAGGTACTGATGCCGCTGGCAATCCAG
                     GTGCATCACGCGGTATGTGACGGCTTCCATGTCGGTCGCATGCTGAACGAGCTGCAGC
                     AATACTGTGATGAATGGCAGGGCGGTGCGTAAACGCGTGGATCCGGCTTACTAAAAGC
                     CAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGAT
                     ATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGAC
                     AGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTC
                     TGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGC
                     GGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTT
                     GCTGACGAGAACAGGGGCTGGTGAAATGCAATTTAAAGTTTATACCTACAAGCGCGAA
                     TCTCGCTACCGTCTGTTTGTAGACGTACAGTCTGATATCATTGATACGCCGGGCCGCC
                     GTATGGTTATTCCGCTGGCATCTGCACGTCTCCTGTCTGATAAAGTTAGCCGTGAACT
                     GTATCCGGTTGTACACATTGGCGACGAGTCTTGGCGCATGATGACGACTGACATGGCT
                     TCTGTCCCGGTTAGCGTCATTGGCGAGGAAGTCGCCGATCTGTCTCACCGTGAAAATG
                     ACATCAAAAATGCAATTAACCTGATGTTCTGGGGCATTTGATCCGGCTTACTAAAAGC
                     CAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGAT
                     ATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGAC
                     AGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTC
                     TGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGC
                     GGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTT
                     GCTGACGAGAACAGGGGCTGGTGAAATGCAATTTAAAGTTTATACCTACAAGCGCGAA
                     TCTCGCTACCGTCTGTTTGTAGACGTACAGTCTGATATCATTGATACGCCGGGCCGCC
                     GTATGGTTATTCCGCTGGCATCTGCACGTCTCCTGTCTGATAAAGTTAGCCGTGAACT
                     GTATCCGGTTGTACACATTGGCGACGAGTCTTGGCGCATGATGACGACTGACATGGCT
                     TCTGTCCCGGTTAGCGTCATTGGCGAGGAAGTCGCCGATCTGTCTCACCGTGAAAATG
                     ACATCAAAAATGCAATTAACCTGATGTTCTGGGGCATTTG"
     misc_feature    7268..7271
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      7272..7717
                     /note="Geneious type: promoter"
                     /note="Bacillus subtilis sacB (glycoside hydrolase family
                     68) promoter region"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB"
     primer_bind     7272..7292
                     /standard_name="1 F"
     gene            7718..9142
                     /note="counterselectable marker from Bacillus subtilis
                     that converts sucrose to levans"
                     /product="levansucrase"
                     /gene="sacB"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB"
     primer_bind     complement(8092..8112)
                     /standard_name="841 R"
     misc_feature    8109..8112
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     8113..8135
                     /standard_name="1 F"
     misc_feature    complement(9178..9301)
                     /note="Geneious type: attR6"
                     /standard_name="attR6"
     misc_feature    9302..9331
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     primer_bind     complement(9307..9331)
                     /standard_name="1,219 R"
     misc_feature    9332..9335
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      complement(9394..9412)
                     /note="Geneious type: Promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7 prom"
     primer_bind     complement(9405..9424)
                     /standard_name="pGGAselect PCR Product (270 R) - pGWB402
                     (digested by PsyI, SapI) Fragment 4"
     misc_feature    9425..9454
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
ORIGIN      
        1 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
       61 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
      121 tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
      181 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
      241 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
      301 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      361 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
      421 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
      481 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
      541 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
      601 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
      661 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
      721 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
      781 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
      841 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
      901 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
      961 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     1021 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1081 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     1141 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     1201 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     1261 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     1321 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca
     1381 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1441 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1501 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1561 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1621 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1681 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1741 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1801 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1861 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1921 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1981 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2041 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2101 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2161 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa atcgtcacct
     2221 gcaggaaggt ttaaacgcat ttaggtgaca ctatagaagt gtgtatcgct cgagggatcc
     2281 gaattcgaag tcttggtacg gaggggatcc gatcgacaag ccgaacatat gggttttttg
     2341 ctcacctgtg attgctccta ctcaaataca aaaacatcaa attttctgtc aataaagcat
     2401 atttatttat atttatttta caggaaagaa ttccttttaa agtgtatttt aacctataat
     2461 gaaaaacgat taaaaaaaat acataaaata attcgaaaat ttttgaatag cccaggttga
     2521 taaaaattca tttcatacgt tttataactt atgcccctaa gtattttttg accatagtgt
     2581 ttcaattcta cattaatttt acagagtaga atgaaacgcc acctactcag ccaagaggcg
     2641 aaaaggttag ctcgccaagc agagagggcg ccagtgctca ctacttttta taattctcaa
     2701 cttctttttc cagactcagt tcgtatatat agacctattt tcaatttaac gtcggggcct
     2761 caactttgta taataaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     2821 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     2881 tatgggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
     2941 ggattttgag ttaggatccg tcgagatttt caggagctac gcggaacccc tatttgttta
     3001 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3061 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3121 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3181 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3241 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3301 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3361 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3421 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3481 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3541 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3601 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3661 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3721 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3781 tctggagccg gtgagcgtgg gtcccgcggt atcattgcag cactggggcc agatggtaag
     3841 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3901 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaatggat ccggcttact
     3961 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     4021 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     4081 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     4141 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     4201 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     4261 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     4321 tatcgtctgt ttgtggatgt acagagtgat attatcgata caccgggccg tcgcatggtt
     4381 attccacttg cgtctgcgcg cttgctctca gataaagtct cccgtgaact ttacccggtg
     4441 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtt
     4501 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     4561 attaacctga tgttctgggg aatataatgt caggctccct tatacacagc cagtctgcac
     4621 gtcgcatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     4681 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa cttttctata
     4741 caaagttgtc cagctgtact gacggacaca ccgaagcccc ggcggcaacc ctcagcggat
     4801 gccccggggc ttcacgtttt cccaggtcag aagcggtttt cgggagtagt gccccaactg
     4861 gggtaacctt tgagttctct cagttggggg cgtagggtcg ccgacatgac acaaggggtt
     4921 gtgaccgggg tggacacgta cgcgggtgct tacgaccgtc agtcgcgcga gcgcgaggtt
     4981 gcttaagaat aagtaattct taagcaacca ttacgccaag cttgcatgca ggcctctgca
     5041 gtcgacgggc ccgggatccg atcgacaagc cgaacatatg ggcgcgccgg gatctaattc
     5101 aattagagac taattcaatt agagctaatt caattaggat ccaagcttat cgatttcgaa
     5161 ccctcgaccg ccggagtata aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa
     5221 acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc gcagctgaac
     5281 aagctaaaca atcggggtac cgctagagtc gacggtaccg cgggcccggg atccaccggt
     5341 cgccaccatg gtgcgctcct ccaagaacgt catcaaggag ttcatgcgct tcaaggtgcg
     5401 catggagggc accgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     5461 ctacgagggc cacaacaccg tgaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
     5521 ctgggacatc ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc
     5581 cgacatcccc gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     5641 gaatttcgaa gatggtggag ttgtcacagt cacacaagat tcttcgctcc aagatggttg
     5701 ttttatttac aaagtgaaat ttattggagt caattttccg tcggatggac cggttatgca
     5761 aaagaaaaca atgggttggg aagcatcgac ggaacgtctg tatccacgag atggagtttt
     5821 gaaaggcgaa attcacaaag ctcttaaact caaagatggc ggacattacc ttgtagaatt
     5881 taaaagcatt tatatggcta agaaaccggt ccaactccct ggatattatt atgtcgattc
     5941 gaaactggat attacttcac ataacgaaga ttatacgata gttgagcaat atgaacgcac
     6001 tgaaggtcgt caccatctct ttctctaaag atcataatca gccataccac atttgtagag
     6061 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     6121 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6181 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6241 ctcatcaatg tatcttatca tgtctggatc gatggcgcgc ctccggaaca taatggtgca
     6301 tctagatgca ttcgcgaggt accgagctcg aattcactgg ggttgcttaa gaataagtaa
     6361 ttcttaagca acccaacttt gtatacaaaa gttgaacgag aaacgtaaaa tgatataaat
     6421 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
     6481 catatccagt cactatgggc cgcattaggc accccaggct ttacacttta tgcttccggc
     6541 tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct
     6601 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     6661 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     6721 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     6781 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
     6841 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     6901 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     6961 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     7021 gagaatatgt ttttcgtgtc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     7081 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     7141 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
     7201 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     7261 taaacgcgtg gcacatatac ctgccgttca ctattattta gtgaaatgag atattatgat
     7321 attttctgaa ttgtgattaa aaaggcaact ttatgcccat gcaacagaaa ctataaaaaa
     7381 tacagagaat gaaaagaaac agatagattt tttagttctt taggcccgta gtctgcaaat
     7441 ccttttatga ttttctatca aacaaaagag gaaaatagac cagttgcaat ccaaacgaga
     7501 gtctaataga atgaggtcga aaagtaaatc gcgcgggttt gttactgata aagcaggcaa
     7561 gacctaaaat gtgtaaaggg caaagtgtat actttggcgt caccccttac atattttagg
     7621 tcttttttta ttgtgcgtaa ctaacttgcc atcttcaaac aggagggctg gaagaagcag
     7681 accgctaaca cagtacataa aaaaggagac atgaacgatg aacatcaaaa agtttgcaaa
     7741 acaagcaaca gtattaacct ttactaccgc actgctggca ggaggcgcaa ctcaagcgtt
     7801 tgcgaaagaa acgaaccaaa agccatataa ggaaacatac ggcatttccc atattacacg
     7861 ccatgatatg ctgcaaatcc ctgaacagca aaaaaatgaa aaatatcaag ttcctgaatt
     7921 cgattcgtcc acaattaaaa atatctcttc tgcaaaaggc ctggacgttt gggacagctg
     7981 gccattacaa aacgctgacg gcactgtcgc aaactatcac ggctaccaca tcgtctttgc
     8041 attagccgga gatcctaaaa atgcggatga cacatcgatt tacatgttct atcaaaaagt
     8101 cggcgaaact tctattgaca gctggaaaaa cgctggccgc gtctttaaag acagcgacaa
     8161 attcgatgca aatgattcta tcctaaaaga ccaaacacaa gaatggtcag gttcagccac
     8221 atttacatct gacggaaaaa tccgtttatt ctacactgat ttctccggta aacattacgg
     8281 caaacaaaca ctgacaactg cacaagttaa cgtatcagca tcagacagct ctttgaacat
     8341 caacggtgta gaggattata aatcaatctt tgacggtgac ggaaaaacgt atcaaaatgt
     8401 acagcagttc atcgatgaag gcaactacag ctcaggcgac aaccatacgc tgagagatcc
     8461 tcactacgta gaagataaag gccacaaata cttagtattt gaagcaaaca ctggaactga
     8521 agatggctac caaggcgaag aatctttatt taacaaagca tactatggca aaagcacatc
     8581 attcttccgt caagaaagtc aaaaacttct gcaaagcgat aaaaaacgca cggctgagtt
     8641 agcaaacggc gctctcggta tgattgagct aaacgatgat tacacactga aaaaagtgat
     8701 gaaaccgctg attgcatcta acacagtaac agatgaaatt gaacgcgcga acgtctttaa
     8761 aatgaacggc aaatggtacc tgttcactga ctcccgcgga tcaaaaatga cgattgacgg
     8821 cattacgtct aacgatattt acatgcttgg ttatgtttct aattctttaa ctggcccata
     8881 caagccgctg aacaaaactg gccttgtgtt aaaaatggat cttgatccta acgatgtaac
     8941 ctttacttac tcacacttcg ctgtacctca agcgaaagga aacaatgtcg tgattacaag
     9001 ctatatgaca aacagaggat tctacgcaga caaacaatca acgtttgcgc caagcttcct
     9061 gctgaacatc aaaggcaaga aaacatctgt tgtcaaagac agcatccttg aacaaggaca
     9121 attaacagtt aacaaataaa tgtcaggctc ccttatacac agccagtctg cacgtcgcat
     9181 agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa
     9241 tttaatatat tgatatttat atcattttac gtttctcgtt caacttttta atacaaagtt
     9301 gcctccggaa cataatggtg catctagatg cccattcctg tagacttctt aattaagacg
     9361 tcagaattct cgaggcggcc gcatgtgagt ctccctatag tgagtcgtat taatttcgcg
     9421 ggcgcgtcag cgggagagcc gttgtaaggc ggca
//
