LOCUS       pJAT30{dU6-3-attR3-Amp-ccdB-attR4-Mhc-DsRed-attR5-Chlor-SacB-attR6-dU6-1}       10496 bp    DNA     circular UNA 12-JUN-2023
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local.sternd.1rt-gdrd5a0
VERSION     urn.local.sternd.1rt-gdrd5a0
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             411..1202
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /standard_name="SmR"
     rep_origin      1448..2036
     misc_feature    1820..1837
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ori-252 R"
     primer_bind     1937..1956
                     /note="pBR322 origin, forward primer"
                     /standard_name="pBR322ori-F"
     primer_bind     2026..2045
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7Cas9F"
     primer_bind     complement(2052..2071)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp-ori-7571F"
     misc_feature    2180..2209
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     2190..2207
                     /note="L4440 vector, forward primer"
                     /standard_name="L4440"
     primer_bind     2210..2229
                     /standard_name="pGGAselect PCR Product (1 F) - pGWB402
                     (digested by PsyI, SapI) Fragment 4"
     regulatory      2239..2258
                     /note="Geneious type: promoter"
                     /note="SP6 RNA polymerase promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SP6"
     misc_feature    2300..2303
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     2304..2322
                     /standard_name="1 F"
     regulatory      2334..2754
                     /note="Geneious type: promoter"
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
                     /standard_name="dU6-3 promoter"
     misc_feature    2758..2787
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2761..2884
                     /note="Geneious type: attR3"
                     /standard_name="attR3"
     primer_bind     complement(2958..2979)
                     /standard_name="676 R"
     misc_feature    2976..2979
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      2980..3084
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     primer_bind     2980..2999
                     /standard_name="1 F"
     misc_feature    3085..3945
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     primer_bind     complement(3125..3146)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AMP_5'_F"
     misc_feature    3331..3350
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_R"
     misc_feature    3804
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="T"
     primer_bind     complement(3817..3836)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_F"
     misc_feature    3909..3932
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp_3'_R"
     primer_bind     complement(3922..3945)
                     /standard_name="966 R"
     misc_feature    3942..3945
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    3946..4585
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGT
                     TGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAAT
                     GAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
                     AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTG
                     AAATGCAATTTAAGGTTTATACTTACAAGCGCGAGTCCCGCTATCGCCTTTTCGTGGA
                     CGTCCAGTCAGACATTATCGACACGCCTGGTCGCCGTATGGTGATTCCATTAGCCAGC
                     GCACGCCTGTTAAGTGATAAGGTGTCGCGCGAGCTTTACCCAGTCGTCCATATTGGTG
                     ACGAGAGTTGGCGCATGATGACTACAGACATGGCTAGTGTACCTGTGTCGGTGATCGG
                     GGAAGAGGTCGCGGATTTAAGCCACCGTGAGAATGATATTAAGAATGCAATCAATCTC
                     ATGTTTTGGGGTATTTA"
     primer_bind     3946..3967
                     /standard_name="1 F"
     gene            4255..4587
                     /standard_name="ccdB"
     misc_feature    complement(4625..4748)
                     /note="Geneious type: attR4"
                     /standard_name="attR4"
     primer_bind     complement(4762..4781)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="2655R"
     primer_bind     complement(4790..4809)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="8,388 F"
     regulatory      complement(4844..5058)
                     /note="Geneious type: Terminator"
                     /standard_name="SV40_PA term"
     primer_bind     complement(4856..4876)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="1198F"
     primer_bind     complement(4905..4924)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40-PA-F"
     misc_feature    4992..5011
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40-PA-R"
     misc_feature    complement(5059..5739)
                     /note="Geneious type: Reporter"
                     /standard_name="DsRed2 reporter"
     primer_bind     5594..5613
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="DsRed-3573R"
     regulatory      complement(5741..5918)
                     /note="Geneious type: Promoter"
                     /standard_name="HS prom"
     misc_feature    5862..5880
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="12878 R"
     misc_feature    5862..5880
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2020.2.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="P693
                     p(MHC-DsRed-CDNopt-5'myr-smGFP-int-B3-B3 recombinase-FSF)
                     - 12878 R (reversed)"
     misc_feature    5904..5928
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    5905..5924
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="4558 R"
     misc_feature    complement(5929..6606)
                     /note="Geneious type: regulatory_region"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Mhc_F4-678"
     primer_bind     6470..6489
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="2647R"
     primer_bind     complement(6585..6606)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="3523R"
     primer_bind     complement(6586..6609)
                     /standard_name="1,863 R"
     misc_feature    6610..6733
                     /note="Geneious type: attR5"
                     /standard_name="attR5"
     misc_feature    6610..6613
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     gene            6840..7499
                     /gene="CAT/CamR"
                     /note="Geneious type: CDS"
                     /standard_name="CAT/CamR"
     misc_feature    6845..7497
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AAAAAAGATTACGGGTTATACTACCGTCGACATCAGCCAGTG
                     GCACCGTAAAGAACATTTCGAAGCGTTCCAGAGCGTCGCGCAGTGCACCTACAATCAG
                     ACCGTTCAGCTGGATATCACTGCTTTCCTGAAGACTGTTAAGAAAAACAAACACAAAT
                     TTTATCCGGCTTTTATCCACATTCTCGCTCGCCTGATGAACGCACACCCGGAATTCCG
                     TATGGCCATGAAAGATGGCGAGCTGGTAATTTGGGATTCCGTTCATCCGTGTTATACC
                     GTCTTTCACGAACAGACCGAGACGTTCTCTTCCCTGTGGTCTG"
     primer_bind     complement(7491..7507)
                     /standard_name="905 R"
     misc_feature    7502..9374
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CGAGACGTTCTCTTCCCTGTGGTCTGAATATCACGATGACTT
                     CCGTCAATTCCTGCACATTTATTCTCAGGACGTTGCGTGCTATGGTGAGAATCTCGCT
                     TATTTTCCGAAGGGTTTCATTGAAAACATGTTCTTTGTTAGCGCCAACCCATGGGTGA
                     GCTTCACTTCTTTCGACCTGAACGTGGCGAACATGGACAACTTTTTCGCGCCGGTTTT
                     CACGATGGGCAAGTATTACACCCAGGGTGACAAGGTACTGATGCCGCTGGCAATCCAG
                     GTGCATCACGCGGTATGTGACGGCTTCCATGTCGGTCGCATGCTGAACGAGCTGCAGC
                     AATACTGTGATGAATGGCAGGGCGGTGCGTAAACGCGTGGATCCGGCTTACTAAAAGC
                     CAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGAT
                     ATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGAC
                     AGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTC
                     TGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGC
                     GGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTT
                     GCTGACGAGAACAGGGGCTGGTGAAATGCAATTTAAAGTTTATACCTACAAGCGCGAA
                     TCTCGCTACCGTCTGTTTGTAGACGTACAGTCTGATATCATTGATACGCCGGGCCGCC
                     GTATGGTTATTCCGCTGGCATCTGCACGTCTCCTGTCTGATAAAGTTAGCCGTGAACT
                     GTATCCGGTTGTACACATTGGCGACGAGTCTTGGCGCATGATGACGACTGACATGGCT
                     TCTGTCCCGGTTAGCGTCATTGGCGAGGAAGTCGCCGATCTGTCTCACCGTGAAAATG
                     ACATCAAAAATGCAATTAACCTGATGTTCTGGGGCATTTGATCCGGCTTACTAAAAGC
                     CAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGAT
                     ATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGAC
                     AGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTC
                     TGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGC
                     GGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTT
                     GCTGACGAGAACAGGGGCTGGTGAAATGCAATTTAAAGTTTATACCTACAAGCGCGAA
                     TCTCGCTACCGTCTGTTTGTAGACGTACAGTCTGATATCATTGATACGCCGGGCCGCC
                     GTATGGTTATTCCGCTGGCATCTGCACGTCTCCTGTCTGATAAAGTTAGCCGTGAACT
                     GTATCCGGTTGTACACATTGGCGACGAGTCTTGGCGCATGATGACGACTGACATGGCT
                     TCTGTCCCGGTTAGCGTCATTGGCGAGGAAGTCGCCGATCTGTCTCACCGTGAAAATG
                     ACATCAAAAATGCAATTAACCTGATGTTCTGGGGCATTTG"
     misc_feature    7504..7507
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      7508..7953
                     /note="Geneious type: promoter"
                     /note="Bacillus subtilis sacB (glycoside hydrolase family
                     68) promoter region"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB"
     primer_bind     7508..7528
                     /standard_name="1 F"
     gene            7954..9376
                     /note="counterselectable marker from Bacillus subtilis
                     that converts sucrose to levans"
                     /product="levansucrase"
                     /gene="sacB"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB CS"
     primer_bind     complement(8328..8348)
                     /standard_name="841 R"
     misc_feature    8345..8348
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     8349..8371
                     /standard_name="1 F"
     misc_feature    complement(9414..9537)
                     /note="Geneious type: attR6"
                     /standard_name="attR6"
     primer_bind     complement(9543..9567)
                     /standard_name="1,219 R"
     misc_feature    9560..9589
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="GTCTTCTCGAAGACCC"
     misc_feature    9560..9589
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    9568..9571
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      complement(9590..10393)
                     /note="Geneious type: promoter"
                     /standard_name="U6:1 promoter"
     primer_bind     10041..10061
                     /standard_name="attB F seq"
     misc_feature    10255
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     misc_feature    10273
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     misc_feature    10282
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     misc_feature    10292
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     misc_feature    10330
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="G"
     misc_feature    10394..10423
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    10394..10423
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      complement(10436..10454)
                     /note="Geneious type: Promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7 prom"
     primer_bind     complement(10447..10466)
                     /standard_name="pGGAselect PCR Product (270 R) - pGWB402
                     (digested by PsyI, SapI) Fragment 4"
     misc_feature    10467..10496
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
ORIGIN      
        1 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
       61 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
      121 tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
      181 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
      241 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
      301 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      361 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
      421 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
      481 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
      541 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
      601 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
      661 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
      721 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
      781 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
      841 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
      901 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
      961 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     1021 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1081 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     1141 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     1201 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     1261 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     1321 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca
     1381 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1441 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1501 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1561 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1621 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1681 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1741 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1801 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1861 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1921 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1981 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2041 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2101 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2161 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa atcgtcacct
     2221 gcaggaaggt ttaaacgcat ttaggtgaca ctatagaagt gtgtatcgct cgagggatcc
     2281 gaattcgaag tcttggtacg gaggggatcc gatcgacaag ccgaacatat gggttttttg
     2341 ctcacctgtg attgctccta ctcaaataca aaaacatcaa attttctgtc aataaagcat
     2401 atttatttat atttatttta caggaaagaa ttccttttaa agtgtatttt aacctataat
     2461 gaaaaacgat taaaaaaaat acataaaata attcgaaaat ttttgaatag cccaggttga
     2521 taaaaattca tttcatacgt tttataactt atgcccctaa gtattttttg accatagtgt
     2581 ttcaattcta cattaatttt acagagtaga atgaaacgcc acctactcag ccaagaggcg
     2641 aaaaggttag ctcgccaagc agagagggcg ccagtgctca ctacttttta taattctcaa
     2701 cttctttttc cagactcagt tcgtatatat agacctattt tcaatttaac gtcggggcct
     2761 caactttgta taataaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     2821 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     2881 tatgggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
     2941 ggattttgag ttaggatccg tcgagatttt caggagctac gcggaacccc tatttgttta
     3001 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3061 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3121 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3181 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3241 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3301 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3361 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3421 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3481 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3541 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3601 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3661 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3721 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3781 tctggagccg gtgagcgtgg gtcccgcggt atcattgcag cactggggcc agatggtaag
     3841 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3901 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaatggat ccggcttact
     3961 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     4021 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     4081 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     4141 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     4201 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     4261 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     4321 tatcgtctgt ttgtggatgt acagagtgat attatcgata caccgggccg tcgcatggtt
     4381 attccacttg cgtctgcgcg cttgctctca gataaagtct cccgtgaact ttacccggtg
     4441 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtt
     4501 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     4561 attaacctga tgttctgggg aatataatgt caggctccct tatacacagc cagtctgcac
     4621 gtcgcatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     4681 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa cttttctata
     4741 caaagttgtc cagctccgag ctcggtacct cgcgaatgca tctagatgca ccattatgtt
     4801 ccggaggcgc gccatcgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     4861 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     4921 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     4981 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     5041 tatggctgat tatgatctct acaggaacag gtggtggcgg ccctcggtgc gctcgtactg
     5101 ctccacgatg gtgtagtcct cgttgtggga ggtgatgtcc agcttggagt ccacgtagta
     5161 gtagccgggc agctgcacgg gcttcttggc catgtagatg gacttgaact ccaccaggta
     5221 gtggccgccg tccttcagct tcagggcctt gtggatctcg cccttcagca cgccgtcgcg
     5281 ggggtacagg cgctcggtgg aggcctccca gcccatggtc ttcttctgca ttacggggcc
     5341 gtcggagggg aagttcacgc cgatgaactt caccttgtag atgaagcagc cgtcctgcag
     5401 ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca
     5461 cttgaagccc tcggggaagg acagcttctt gtagtcgggg atgtcggcgg ggtgcttcac
     5521 gtacaccttg gagccgtact ggaactgggg ggacaggatg tcccaggcga agggcagggg
     5581 gccgcccttg gtcaccttca gcttcacggt gttgtggccc tcgtaggggc ggccctcgcc
     5641 ctcgccctcg atctcgaact cgtggccgtt cacggtgccc tccatgcgca ccttgaagcg
     5701 catgaactcc ttgatgacgt tcttggagga gcgcaccatg gtggcgaccg gtggatcccg
     5761 ggcccgcggt accgtcgact ctagcggtac cccgattgtt tagcttgttc agctgcgctt
     5821 gtttatttgc ttagctttcg cttagcgacg tgttcacttt gcttgtttga attgaattgt
     5881 cgctccgtag acgaagcgcc tctatttata ctccggcggt cgagggttcc taaatataga
     5941 tcacggatcc gatacgaata tgtatatgta taccacttat atatatatat atcagaatgc
     6001 tttgcatata tacggatttt cggggcgttg gtatttgtta cgtaacgcca attttcattt
     6061 agatatttgt gtacatttct ccaggtttct ttgtgaaaaa atctacagac tattgatacg
     6121 ccaaaacaat gcttattatt acaaatgtag agaggttaaa ctgtcaccac aactcactca
     6181 actggtaact actaccgttt ggaacgcgat ttcgatattt tgaacaggta tctcgatatg
     6241 gtggcgtgtt ctcatacaag caattcacga gatcgcaatg attcctttca gtaaacttcg
     6301 aaggcggaac gattttcgac aatccacact aaatcgacaa tcgaaaattc gattcgaatt
     6361 gacgcgaact tttaacgaat gcgttatttt taaaattctc gaaattattt cgaaactaaa
     6421 gtcacataca tattcgtcta tatgtaattc gtaatttcga gaagcttttt ggctcatgct
     6481 accacatggc acacatagac ataatgtata agtattggcg atctctttct aatgtctttg
     6541 gttattttta aaacatattc gaaattattt cgcccatgta tatgagccga caacgaattc
     6601 gagtataatc aactttgtat acaaaagttg aacgagaaac gtaaaatgat ataaatatca
     6661 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
     6721 tccagtcact atgggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
     6781 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
     6841 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     6901 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     6961 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     7021 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     7081 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     7141 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     7201 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     7261 atatgttttt cgtgtcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     7321 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     7381 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     7441 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
     7501 cgcgtggcac atatacctgc cgttcactat tatttagtga aatgagatat tatgatattt
     7561 tctgaattgt gattaaaaag gcaactttat gcccatgcaa cagaaactat aaaaaataca
     7621 gagaatgaaa agaaacagat agatttttta gttctttagg cccgtagtct gcaaatcctt
     7681 ttatgatttt ctatcaaaca aaagaggaaa atagaccagt tgcaatccaa acgagagtct
     7741 aatagaatga ggtcgaaaag taaatcgcgc gggtttgtta ctgataaagc aggcaagacc
     7801 taaaatgtgt aaagggcaaa gtgtatactt tggcgtcacc ccttacatat tttaggtctt
     7861 tttttattgt gcgtaactaa cttgccatct tcaaacagga gggctggaag aagcagaccg
     7921 ctaacacagt acataaaaaa ggagacatga acgatgaaca tcaaaaagtt tgcaaaacaa
     7981 gcaacagtat taacctttac taccgcactg ctggcaggag gcgcaactca agcgtttgcg
     8041 aaagaaacga accaaaagcc atataaggaa acatacggca tttcccatat tacacgccat
     8101 gatatgctgc aaatccctga acagcaaaaa aatgaaaaat atcaagttcc tgaattcgat
     8161 tcgtccacaa ttaaaaatat ctcttctgca aaaggcctgg acgtttggga cagctggcca
     8221 ttacaaaacg ctgacggcac tgtcgcaaac tatcacggct accacatcgt ctttgcatta
     8281 gccggagatc ctaaaaatgc ggatgacaca tcgatttaca tgttctatca aaaagtcggc
     8341 gaaacttcta ttgacagctg gaaaaacgct ggccgcgtct ttaaagacag cgacaaattc
     8401 gatgcaaatg attctatcct aaaagaccaa acacaagaat ggtcaggttc agccacattt
     8461 acatctgacg gaaaaatccg tttattctac actgatttct ccggtaaaca ttacggcaaa
     8521 caaacactga caactgcaca agttaacgta tcagcatcag acagctcttt gaacatcaac
     8581 ggtgtagagg attataaatc aatctttgac ggtgacggaa aaacgtatca aaatgtacag
     8641 cagttcatcg atgaaggcaa ctacagctca ggcgacaacc atacgctgag agatcctcac
     8701 tacgtagaag ataaaggcca caaatactta gtatttgaag caaacactgg aactgaagat
     8761 ggctaccaag gcgaagaatc tttatttaac aaagcatact atggcaaaag cacatcattc
     8821 ttccgtcaag aaagtcaaaa acttctgcaa agcgataaaa aacgcacggc tgagttagca
     8881 aacggcgctc tcggtatgat tgagctaaac gatgattaca cactgaaaaa agtgatgaaa
     8941 ccgctgattg catctaacac agtaacagat gaaattgaac gcgcgaacgt ctttaaaatg
     9001 aacggcaaat ggtacctgtt cactgactcc cgcggatcaa aaatgacgat tgacggcatt
     9061 acgtctaacg atatttacat gcttggttat gtttctaatt ctttaactgg cccatacaag
     9121 ccgctgaaca aaactggcct tgtgttaaaa atggatcttg atcctaacga tgtaaccttt
     9181 acttactcac acttcgctgt acctcaagcg aaaggaaaca atgtcgtgat tacaagctat
     9241 atgacaaaca gaggattcta cgcagacaaa caatcaacgt ttgcgccaag cttcctgctg
     9301 aacatcaaag gcaagaaaac atctgttgtc aaagacagca tccttgaaca aggacaatta
     9361 acagttaaca aataaatgtc aggctccctt atacacagcc agtctgcacg tcgcatagtg
     9421 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
     9481 atatattgat atttatatca ttttacgttt ctcgttcaac tttttaatac aaagttgcct
     9541 ccggaacata atggtgcatc tagatgccca ttcctgtaga cttcttaatc gaagttcacc
     9601 cggatatctt tcctatatat actgtactct tgcagaatat gtacttggga atcataagga
     9661 tgcgaaggac gtacgcgctg tgtctgtaac taaggggttg ctttcgggta cctaccatgc
     9721 caatgatggg actaccgccg gataggaatc ccttttgggt gcacaccgtc acctccagtt
     9781 ccgtctgcct gagttcggca atggaaatca ggtactcaaa ggatgcatcg tagacggggt
     9841 tgcagttgtc cttgatcacg ctcgtcttgc gtttcgactc cttggtgcgt ccaggcaaca
     9901 gatacagctt aacatacgga tcggggatat tgctgggatc gcgaagtggt atcttctgga
     9961 ttttgtgtat ggtcacgtct agtttttgac gctgggcgct gtagcggatc gacaactgca
    10021 ttcgtccaag gccatgttcg ccgggtgagg aggtggagtc tggaaagcgg tgggtcagca
    10081 cagaggtggc cagctcgctg atgggttttt cgctggaggt ggaggccgac atggccgggg
    10141 agattttcgt agccgccaca ggatcttctt cactgatggg cgattcggcg gacaaacgct
    10201 tgctggatgc ctgagattcc taaaagcaat ggcaaattag attcaattaa aacagaaatt
    10261 gtttatataa cagagtaaaa gcaaaaaaaa aggaatcaca gattctcccc gacggggaat
    10321 tgaaccccgt tctcccgcgt gacaggcggg gatactaacc actatactat cgaggacgtt
    10381 gaaaatgaat tcaattaaga cgtcagaatt ctcgaggcgg ccgcatgtga gtctccctat
    10441 agtgagtcgt attaatttcg cgggcgcgtc agcgggagag ccgttgtaag gcggca
//
