LOCUS       pJAT77{attR3-Amp-ccdB-attR4-MCS-attR5-Chlor-SacB-attR6}        7693 bp    DNA     circular UNA 12-JUN-2023
DEFINITION  Generated by Gibson/Homology Cloning (P1176
            p{GW1-Amp-ccdB-MCS-GW2-Chlor-SacB} (digested by PspXI),
            Apis_U6_prom).
ACCESSION   urn.local.sternd.1ta-gdrdar0
VERSION     urn.local.sternd.1ta-gdrdar0
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    6..129
                     /note="Geneious type: attR3"
                     /standard_name="attR3"
     regulatory      152..182
                     /note="Geneious type: promoter"
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacUV5"
     misc_feature    complement(163..174)
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     misc_feature    221..224
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      225..329
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     gene            330..>1048
                     /standard_name="AmpR"
     primer_bind     complement(370..391)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AMP_5'_F"
     misc_feature    576..595
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_R"
     gene            <1046..1190
                     /standard_name="AmpR"
     misc_feature    1046..1048
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    1049
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="T"
     primer_bind     complement(1062..1081)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_F"
     misc_feature    1154..1177
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp_3'_R"
     primer_bind     complement(1167..1190)
                     /standard_name="966 R"
     misc_feature    1187..1190
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    1191..1830
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGT
                     TGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAAT
                     GAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
                     AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTG
                     AAATGCAATTTAAGGTTTATACTTACAAGCGCGAGTCCCGCTATCGCCTTTTCGTGGA
                     CGTCCAGTCAGACATTATCGACACGCCTGGTCGCCGTATGGTGATTCCATTAGCCAGC
                     GCACGCCTGTTAAGTGATAAGGTGTCGCGCGAGCTTTACCCAGTCGTCCATATTGGTG
                     ACGAGAGTTGGCGCATGATGACTACAGACATGGCTAGTGTACCTGTGTCGGTGATCGG
                     GGAAGAGGTCGCGGATTTAAGCCACCGTGAGAATGATATTAAGAATGCAATCAATCTC
                     ATGTTTTGGGGTATTTA"
     primer_bind     1191..1212
                     /standard_name="1 F"
     gene            1500..1832
                     /standard_name="ccdB"
     primer_bind     1691..1710
                     /standard_name="4446F"
     misc_feature    complement(1870..1993)
                     /note="Geneious type: attR4"
                     /standard_name="attR4"
     primer_bind     1986..2005
                     /standard_name="4741F"
     misc_feature    1997..2000
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2001..2004
                     /note="4 base BsaI overhang created during Golden Gate
                     assembly (BsaI site 320-325)"
                     /standard_name="BsaI overhang"
     misc_feature    2005..2146
                     /note="DNA sequence removed and replaced during Golden
                     Gate assembly with BsaI"
                     /standard_name="Fragment removed by BsaI"
     misc_feature    2006..2011
                     /standard_name="BsaI"
     misc_feature    2012..2015
                     /note="4 base BbsI overhang created during Golden Gate
                     assembly (BbsI site 343-348)"
                     /standard_name="BbsI overhang"
     misc_feature    2016..2135
                     /note="DNA sequence removed and replaced during Golden
                     Gate assembly with BbsI"
                     /standard_name="Fragment removed by BbsI"
     misc_feature    2018..2023
                     /standard_name="BbsI"
     misc_feature    2031..2069
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     primer_bind     complement(2041..2060)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="2655R"
     misc_feature    2076..2117
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    complement(2128..2133)
                     /standard_name="BbsI"
     primer_bind     complement(2132..2151)
                     /standard_name="4887R"
     misc_feature    complement(2136..2139)
                     /note="4 base BbsI overhang created during Golden Gate
                     assembly (BbsI site 372-377)"
                     /standard_name="BbsI overhang"
     misc_feature    complement(2140..2145)
                     /standard_name="BsaI"
     misc_feature    complement(2147..2150)
                     /note="4 base BsaI overhang created during Golden Gate
                     assembly (BsaI site 395-400)"
                     /standard_name="BsaI overhang"
     misc_feature    2151..2274
                     /note="Geneious type: attR5"
                     /standard_name="attR5"
     misc_feature    2151..2154
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     gene            2381..3040
                     /gene="CAT/CamR"
                     /standard_name="CAT/CamR"
     misc_feature    2386..3038
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AAAAAAGATTACGGGTTATACTACCGTCGACATCAGCCAGTG
                     GCACCGTAAAGAACATTTCGAAGCGTTCCAGAGCGTCGCGCAGTGCACCTACAATCAG
                     ACCGTTCAGCTGGATATCACTGCTTTCCTGAAGACTGTTAAGAAAAACAAACACAAAT
                     TTTATCCGGCTTTTATCCACATTCTCGCTCGCCTGATGAACGCACACCCGGAATTCCG
                     TATGGCCATGAAAGATGGCGAGCTGGTAATTTGGGATTCCGTTCATCCGTGTTATACC
                     GTCTTTCACGAACAGACCGAGACGTTCTCTTCCCTGTGGTCTG"
     primer_bind     complement(2479..2498)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="10,588 R"
     misc_feature    3043..4915
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CGAGACGTTCTCTTCCCTGTGGTCTGAATATCACGATGACTT
                     CCGTCAATTCCTGCACATTTATTCTCAGGACGTTGCGTGCTATGGTGAGAATCTCGCT
                     TATTTTCCGAAGGGTTTCATTGAAAACATGTTCTTTGTTAGCGCCAACCCATGGGTGA
                     GCTTCACTTCTTTCGACCTGAACGTGGCGAACATGGACAACTTTTTCGCGCCGGTTTT
                     CACGATGGGCAAGTATTACACCCAGGGTGACAAGGTACTGATGCCGCTGGCAATCCAG
                     GTGCATCACGCGGTATGTGACGGCTTCCATGTCGGTCGCATGCTGAACGAGCTGCAGC
                     AATACTGTGATGAATGGCAGGGCGGTGCGTAAACGCGTGGATCCGGCTTACTAAAAGC
                     CAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGAT
                     ATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGAC
                     AGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTC
                     TGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGC
                     GGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTT
                     GCTGACGAGAACAGGGGCTGGTGAAATGCAATTTAAAGTTTATACCTACAAGCGCGAA
                     TCTCGCTACCGTCTGTTTGTAGACGTACAGTCTGATATCATTGATACGCCGGGCCGCC
                     GTATGGTTATTCCGCTGGCATCTGCACGTCTCCTGTCTGATAAAGTTAGCCGTGAACT
                     GTATCCGGTTGTACACATTGGCGACGAGTCTTGGCGCATGATGACGACTGACATGGCT
                     TCTGTCCCGGTTAGCGTCATTGGCGAGGAAGTCGCCGATCTGTCTCACCGTGAAAATG
                     ACATCAAAAATGCAATTAACCTGATGTTCTGGGGCATTTGATCCGGCTTACTAAAAGC
                     CAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGAT
                     ATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGAC
                     AGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTC
                     TGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGC
                     GGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTT
                     GCTGACGAGAACAGGGGCTGGTGAAATGCAATTTAAAGTTTATACCTACAAGCGCGAA
                     TCTCGCTACCGTCTGTTTGTAGACGTACAGTCTGATATCATTGATACGCCGGGCCGCC
                     GTATGGTTATTCCGCTGGCATCTGCACGTCTCCTGTCTGATAAAGTTAGCCGTGAACT
                     GTATCCGGTTGTACACATTGGCGACGAGTCTTGGCGCATGATGACGACTGACATGGCT
                     TCTGTCCCGGTTAGCGTCATTGGCGAGGAAGTCGCCGATCTGTCTCACCGTGAAAATG
                     ACATCAAAAATGCAATTAACCTGATGTTCTGGGGCATTTG"
     misc_feature    3045..3048
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      3049..3494
                     /note="Geneious type: promoter"
                     /note="Bacillus subtilis sacB (glycoside hydrolase family
                     68) promoter region"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB"
     gene            3495..4918
                     /note="counterselectable marker from Bacillus subtilis
                     that converts sucrose to levans"
                     /product="levansucrase"
                     /gene="sacB"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB CS"
     misc_feature    3886..3889
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    complement(4955..5078)
                     /note="Geneious type: attR6"
                     /standard_name="attR6"
     misc_feature    5079..5108
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     primer_bind     complement(5084..5107)
                     /standard_name="P859-5,113R"
     misc_feature    5109..5112
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      complement(5171..5189)
                     /note="Geneious type: Promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7 prom"
     regulatory      complement(5172..5189)
                     /regulatory_class="promoter"
                     /note="T7 promoter"
                     /standard_name="T7 promoter"
     misc_feature    5202..5231
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     CDS             5642..6433
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /standard_name="SmR"
     rep_origin      6679..7267
     misc_feature    7051..7068
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ori-252 R"
     primer_bind     7168..7187
                     /note="pBR322 origin, forward primer"
                     /standard_name="pBR322ori-F"
     primer_bind     7257..7276
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7Cas9F"
     primer_bind     complement(7283..7302)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp-ori-7571F"
     misc_feature    7411..7440
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     7421..7438
                     /note="L4440 vector, forward primer"
                     /standard_name="L4440"
     regulatory      7470..7489
                     /note="Geneious type: promoter"
                     /note="SP6 RNA polymerase promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SP6"
     misc_feature    7471..7500
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    7471..7667
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Apis_U6_prom (concatenated sequence 2)"
     misc_feature    7471..7500
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     regulatory      7501..7637
                     /note="Geneious type: promoter"
                     /standard_name="U6_prom"
     misc_feature    7638..7667
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    7638..7667
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    7638..7500
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P1176 p{GW1-Amp-ccdB-MCS-GW2-Chlor-SacB}
                     (digested by PspXI) (concatenated sequence 1)"
ORIGIN      
        1 ggcctcaact ttgtataata aagttgaacg agaaacgtaa aatgatataa atatcaatat
       61 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
      121 gtcactatgg gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa
      181 tgtgtggatt ttgagttagg atccgtcgag attttcagga gctacgcgga acccctattt
      241 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      301 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      361 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      421 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      481 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
      541 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
      601 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
      661 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
      721 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
      781 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
      841 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
      901 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
      961 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     1021 ataaatctgg agccggtgag cgtgggtccc gcggtatcat tgcagcactg gggccagatg
     1081 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1141 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa tggatccggc
     1201 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
     1261 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
     1321 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
     1381 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
     1441 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
     1501 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
     1561 gccgttatcg tctgtttgtg gatgtacaga gtgatattat cgatacaccg ggccgtcgca
     1621 tggttattcc acttgcgtct gcgcgcttgc tctcagataa agtctcccgt gaactttacc
     1681 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
     1741 cggtttccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
     1801 acgccattaa cctgatgttc tggggaatat aatgtcaggc tcccttatac acagccagtc
     1861 tgcacgtcgc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1921 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcaactttt
     1981 ctatacaaag ttgtccagct ggagcgagac cggagtcgtc ttcgctttcc gaattcgagc
     2041 tcggtacctc gcgaatgcat ctagatatca gatctggatc ccgggcccgt cgactgcaga
     2101 ggcctgcatg caagcttgat aacttgtgaa gacgaccatg gtctcaccat caactttgta
     2161 tacaaaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     2221 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatgggccgc
     2281 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
     2341 ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     2401 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     2461 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     2521 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     2581 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
     2641 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     2701 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     2761 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtgtcagc
     2821 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     2881 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     2941 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     3001 attacaacag tactgcgatg agtggcaggg cggggcgtaa acgcgtggca catatacctg
     3061 ccgttcacta ttatttagtg aaatgagata ttatgatatt ttctgaattg tgattaaaaa
     3121 ggcaacttta tgcccatgca acagaaacta taaaaaatac agagaatgaa aagaaacaga
     3181 tagatttttt agttctttag gcccgtagtc tgcaaatcct tttatgattt tctatcaaac
     3241 aaaagaggaa aatagaccag ttgcaatcca aacgagagtc taatagaatg aggtcgaaaa
     3301 gtaaatcgcg cgggtttgtt actgataaag caggcaagac ctaaaatgtg taaagggcaa
     3361 agtgtatact ttggcgtcac cccttacata ttttaggtct ttttttattg tgcgtaacta
     3421 acttgccatc ttcaaacagg agggctggaa gaagcagacc gctaacacag tacataaaaa
     3481 aggagacatg aacgatgaac atcaaaaagt ttgcaaaaca agcaacagta ttaaccttta
     3541 ctaccgcact gctggcagga ggcgcaactc aagcgtttgc gaaagaaacg aaccaaaagc
     3601 catataagga aacatacggc atttcccata ttacacgcca tgatatgctg caaatccctg
     3661 aacagcaaaa aaatgaaaaa tatcaagttc ctgaattcga ttcgtccaca attaaaaata
     3721 tctcttctgc aaaaggcctg gacgtttggg acagctggcc attacaaaac gctgacggca
     3781 ctgtcgcaaa ctatcacggc taccacatcg tctttgcatt agccggagat cctaaaaatg
     3841 cggatgacac atcgatttac atgttctatc aaaaagtcgg cgaaacttct attgacagct
     3901 ggaaaaacgc tggccgcgtc tttaaagaca gcgacaaatt cgatgcaaat gattctatcc
     3961 taaaagacca aacacaagaa tggtcaggtt cagccacatt tacatctgac ggaaaaatcc
     4021 gtttattcta cactgatttc tccggtaaac attacggcaa acaaacactg acaactgcac
     4081 aagttaacgt atcagcatca gacagctctt tgaacatcaa cggtgtagag gattataaat
     4141 caatctttga cggtgacgga aaaacgtatc aaaatgtaca gcagttcatc gatgaaggca
     4201 actacagctc aggcgacaac catacgctga gagatcctca ctacgtagaa gataaaggcc
     4261 acaaatactt agtatttgaa gcaaacactg gaactgaaga tggctaccaa ggcgaagaat
     4321 ctttatttaa caaagcatac tatggcaaaa gcacatcatt cttccgtcaa gaaagtcaaa
     4381 aacttctgca aagcgataaa aaacgcacgg ctgagttagc aaacggcgct ctcggtatga
     4441 ttgagctaaa cgatgattac acactgaaaa aagtgatgaa accgctgatt gcatctaaca
     4501 cagtaacaga tgaaattgaa cgcgcgaacg tctttaaaat gaacggcaaa tggtacctgt
     4561 tcactgactc ccgcggatca aaaatgacga ttgacggcat tacgtctaac gatatttaca
     4621 tgcttggtta tgtttctaat tctttaactg gcccatacaa gccgctgaac aaaactggcc
     4681 ttgtgttaaa aatggatctt gatcctaacg atgtaacctt tacttactca cacttcgctg
     4741 tacctcaagc gaaaggaaac aatgtcgtga ttacaagcta tatgacaaac agaggattct
     4801 acgcagacaa acaatcaacg tttgcgccaa gcttcctgct gaacatcaaa ggcaagaaaa
     4861 catctgttgt caaagacagc atccttgaac aaggacaatt aacagttaac aaataaatgt
     4921 caggctccct tatacacagc cagtctgcac gtcgcatagt gactggatat gttgtgtttt
     4981 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
     5041 attttacgtt tctcgttcaa ctttttaata caaagttgcc tccggaacat aatggtgcat
     5101 ctagatgccc attcctgtag acttcttaat taagacgtca gaattctcga ggcggccgca
     5161 tgtgagtctc cctatagtga gtcgtattaa tttcgcgggc gcgtcagcgg gagagccgtt
     5221 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
     5281 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
     5341 gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg
     5401 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     5461 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     5521 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     5581 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     5641 catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     5701 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     5761 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     5821 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     5881 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     5941 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     6001 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     6061 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     6121 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     6181 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     6241 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     6301 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     6361 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     6421 agtcggcaaa taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc
     6481 ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa
     6541 gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc
     6601 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6661 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6721 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6781 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     6841 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6901 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6961 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7021 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7081 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7141 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7201 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7261 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7321 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     7381 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     7441 aatcgtcacc tgcaggaagg tttaaacgca tttaggtgac actatagaag tgtgtatcgc
     7501 ctagctctat ctagtggcga tttactttcg gtcagaaggt caattgttat atttcgaata
     7561 aatattatca attctcatat gtgatgttac aaacatcatt actttatata tatactacgt
     7621 ttgatacggt atcgaatggg atccgaattc gaagtcttgg tacggagggg atccgatcga
     7681 caagccgaac ata
//
