LOCUS       pJAT56{dU6-3-attR3-Amp-ccdB-attR4-vas-phiC31-vasUTR,_3XP3-EGFP-attR5-Chlor-SacB-attR6}       13669 bp    DNA     circular UNA 12-JUN-2023
DEFINITION  Generated by Golden Gate/Type IIS Cloning (P859
            p{dU6-3-GW1-Amp-ccdB-MCS-GW2-Chlor-SacB}, P53
            pBac{vas-phiC31-vasUTR, 3XP3-EGFP} PCR Product).
ACCESSION   urn.local.sternd.1vi-gdrtn57
VERSION     urn.local.sternd.1vi-gdrtn57
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..4759
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P859
                     p{dU6-3-GW1-Amp-ccdB-MCS-GW2-Chlor-SacB} extraction
                     (concatenated sequence 1)"
     CDS             411..1202
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /standard_name="SmR"
     rep_origin      1448..2036
     misc_feature    1820..1837
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ori-252 R"
     primer_bind     1937..1956
                     /note="pBR322 origin, forward primer"
                     /standard_name="pBR322ori-F"
     primer_bind     2026..2045
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7Cas9F"
     primer_bind     complement(2052..2071)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp-ori-7571F"
     misc_feature    2180..2209
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     2190..2207
                     /note="L4440 vector, forward primer"
                     /standard_name="L4440"
     regulatory      2239..2258
                     /note="Geneious type: promoter"
                     /note="SP6 RNA polymerase promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SP6"
     misc_feature    2300..2303
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2304..2333
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     regulatory      2334..2754
                     /note="Geneious type: promoter"
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
                     /standard_name="dU6-3 promoter"
     misc_feature    complement(2399..2409)
                     /note="Geneious type: Transcription Factor"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Hb"
     misc_feature    2755..2787
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    2758..2787
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2761..2884
                     /note="Geneious type: attR3"
                     /standard_name="attR3"
     regulatory      2907..2937
                     /note="Geneious type: promoter"
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacUV5"
     misc_feature    complement(2918..2929)
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     misc_feature    2976..2979
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      2980..3084
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     gene            3085..3945
                     /standard_name="AmpR"
     primer_bind     complement(3125..3146)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AMP_5'_F"
     misc_feature    3331..3350
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_R"
     misc_feature    3804
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="T"
     primer_bind     complement(3817..3836)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_F"
     misc_feature    3909..3932
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp_3'_R"
     primer_bind     complement(3922..3945)
                     /standard_name="966 R"
     misc_feature    3942..3945
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     3946..3967
                     /standard_name="1 F"
     gene            4255..4587
                     /standard_name="ccdB"
     primer_bind     4446..4465
                     /standard_name="4446F"
     misc_feature    complement(4625..4748)
                     /note="Geneious type: attR4"
                     /standard_name="attR4"
     misc_feature    join(4752..>4759,<10585..10592)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pGGAselect-Spect extraction (concatenated
                     sequence 2)"
     misc_feature    4752..4755
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    4756..4759
                     /note="4 base BsaI overhang created during Golden Gate
                     assembly (BsaI site 320-325)"
                     /standard_name="BsaI overhang"
     misc_feature    4756..10588
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P53 pBac{vas-phiC31-vasUTR, 3XP3-EGFP} PCR
                     Product (concatenated sequence 2)"
     misc_feature    join(<4756..>4759,<10585..>10588)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pGGAselect PCR Product (concatenated
                     sequence 2)"
     misc_feature    4756..4759
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     4760..4781
                     /standard_name="1 F"
     misc_feature    4789..4801
                     /note="Geneious type: dna binding region"
                     /standard_name="P3"
     misc_feature    4805..4817
                     /note="Geneious type: dna binding region"
                     /standard_name="P3"
     misc_feature    4820..4832
                     /note="Geneious type: dna binding region"
                     /standard_name="P3"
     regulatory      4864..5042
                     /note="Geneious type: promoter"
                     /note="Derived using Geneious Prime 2020.2.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="HS promoter"
     misc_feature    5043..5759
                     /note="Geneious type: Reporter"
                     /standard_name="EGFP"
     regulatory      5778..6022
                     /note="Geneious type: Terminator"
                     /standard_name="SV40_PA term"
     regulatory      6023..8292
                     /note="Geneious type: promoter"
                     /standard_name="vas promoter"
     CDS             8293..10110
                     /standard_name="phiC31"
     3'UTR           10117..10264
                     /standard_name="vas 3'UTR"
     misc_feature    10117..10584
                     /standard_name="2L 3' vas"
     primer_bind     complement(10569..10584)
                     /standard_name="5,825 R"
     misc_feature    complement(10585..10588)
                     /note="4 base BsaI overhang created during Golden Gate
                     assembly (BsaI site 395-400)"
                     /standard_name="BsaI overhang"
     misc_feature    10585..13669
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P859
                     p{dU6-3-GW1-Amp-ccdB-MCS-GW2-Chlor-SacB} extraction
                     (concatenated sequence 3)"
     misc_feature    10585..10588
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    10589..10712
                     /note="Geneious type: attR5"
                     /standard_name="attR5"
     misc_feature    10589..4755
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P767
                     p{dU6-3-GW1-Amp-ccdB-Mhc-GFP-FRT-P3-DsRed-GW2-Chlor-SacB}
                     (concatenated sequence 1)"
     misc_feature    10589..10592
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     gene            10819..11478
                     /gene="CAT/CamR"
                     /standard_name="CAT/CamR"
     primer_bind     complement(10917..10936)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="10,588 R"
     misc_feature    11483..11486
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      11487..11932
                     /note="Geneious type: promoter"
                     /note="Bacillus subtilis sacB (glycoside hydrolase family
                     68) promoter region"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB"
     gene            11933..13356
                     /note="counterselectable marker from Bacillus subtilis
                     that converts sucrose to levans"
                     /product="levansucrase"
                     /gene="sacB"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB CS"
     misc_feature    12324..12327
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    complement(13393..13516)
                     /note="Geneious type: attR6"
                     /standard_name="attR6"
     misc_feature    13517..13546
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    13547..13550
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      complement(13609..13627)
                     /note="Geneious type: Promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7 prom"
     regulatory      complement(13610..13627)
                     /regulatory_class="promoter"
                     /note="T7 promoter"
                     /standard_name="T7 promoter"
     misc_feature    13640..13669
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
ORIGIN      
        1 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
       61 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
      121 tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
      181 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
      241 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
      301 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      361 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
      421 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
      481 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
      541 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
      601 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
      661 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
      721 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
      781 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
      841 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
      901 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
      961 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     1021 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1081 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     1141 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     1201 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     1261 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     1321 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca
     1381 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1441 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1501 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1561 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1621 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1681 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1741 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1801 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1861 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1921 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1981 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2041 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2101 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2161 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa atcgtcacct
     2221 gcaggaaggt ttaaacgcat ttaggtgaca ctatagaagt gtgtatcgct cgagggatcc
     2281 gaattcgaag tcttggtacg gaggggatcc gatcgacaag ccgaacatat gggttttttg
     2341 ctcacctgtg attgctccta ctcaaataca aaaacatcaa attttctgtc aataaagcat
     2401 atttatttat atttatttta caggaaagaa ttccttttaa agtgtatttt aacctataat
     2461 gaaaaacgat taaaaaaaat acataaaata attcgaaaat ttttgaatag cccaggttga
     2521 taaaaattca tttcatacgt tttataactt atgcccctaa gtattttttg accatagtgt
     2581 ttcaattcta cattaatttt acagagtaga atgaaacgcc acctactcag ccaagaggcg
     2641 aaaaggttag ctcgccaagc agagagggcg ccagtgctca ctacttttta taattctcaa
     2701 cttctttttc cagactcagt tcgtatatat agacctattt tcaatttaac gtcggggcct
     2761 caactttgta taataaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     2821 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     2881 tatgggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
     2941 ggattttgag ttaggatccg tcgagatttt caggagctac gcggaacccc tatttgttta
     3001 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3061 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3121 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3181 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3241 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3301 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3361 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3421 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3481 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3541 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3601 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3661 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3721 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3781 tctggagccg gtgagcgtgg gtcccgcggt atcattgcag cactggggcc agatggtaag
     3841 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3901 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaatggat ccggcttact
     3961 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     4021 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     4081 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     4141 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     4201 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     4261 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     4321 tatcgtctgt ttgtggatgt acagagtgat attatcgata caccgggccg tcgcatggtt
     4381 attccacttg cgtctgcgcg cttgctctca gataaagtct cccgtgaact ttacccggtg
     4441 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtt
     4501 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     4561 attaacctga tgttctgggg aatataatgt caggctccct tatacacagc cagtctgcac
     4621 gtcgcatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     4681 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa cttttctata
     4741 caaagttgtc cagctggagc aatggttaat tcgagctcgc ccggggatct aattcaatta
     4801 gagactaatt caattagagc taattcaatt aggatccaag cttatcgatt tcgaaccctc
     4861 gaccgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa ttcaaacaag
     4921 caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc tgaacaagct
     4981 aaacaatcgg ggtaccgcta gagtcgacgg taccgcgggc ccgggatcca ccggtcgcca
     5041 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     5101 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     5161 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     5221 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     5281 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     5341 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     5401 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     5461 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     5521 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     5581 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     5641 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     5701 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     5761 aaagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     5821 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     5881 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5941 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6001 atcttaaagc ttatcgatac gcgtacggcg cgcgaggtcg acggtatcga taagcttgat
     6061 atcgaattcc tgcagctggt tgtaggtgca gttgcgcttc ttgcacttgg cgcacttgag
     6121 gagatcggtc ttggtgccct gcacggtggc cagctgggca tcgttgatgg cctccttgac
     6181 gaacttctcg cgcagcttct tcatctcgtc gctggccatc tcctccggcg tcattttggc
     6241 cagctgcttg gcagtgacgg cgccgcacat aaagttgccg cgcaatccag gattcttggg
     6301 gtccttcaga ttggccacgc gtgacctaat gcgattcttg tacttcatat ccgtgttatt
     6361 gaactcggag taaatggcat cctccagttc ggcggccatt tcctccggct caccgcatcc
     6421 ctcgggcact tcgccaatct tcagtgcggt ggccagcatt tcgcggcatt tgatgcggac
     6481 cgcatctgtc atgccaccgg agggaaacga ggtctgtgac gaggatgtcg agcctttctt
     6541 ctctttatcc ttggatgagc tggatgaact ggacttatcc ttgccggaga ttgacgacga
     6601 cgacttggcg gcactggtgg acttcgaggc actgctgttg ttggaggagc cctccttagc
     6661 ggagctgttg ttcggtgtgg ttggcgccgg gctggccagg aagcgtttcc agttcttgat
     6721 cagcgtcttg gccagagcga tcacctcgtc gtccttgcta ctcttgcgca gctcgtttac
     6781 ggtcatgccg atgcgcgttt tggtcagaat gtcgagattg atgttaagcg tttgcagggc
     6841 cttcagcagg tccagagcct gatcctgtcc ctgtacggtg gatatatgta tatttgtgac
     6901 atgatgcgcg gcacgttagc taatcccgat acttactgtg ccgtcgctgg ccatcttgct
     6961 catcttcttt tggattcgaa acacttcctc ctccacgctc attttggccc acttaatttg
     7021 atcagaggtt aggttaactg gcgtcggctg tcggctttaa tcggtgctgc gggtctgcga
     7081 cagttgttta tgcggaagcc gggctatttc tttttttccc tccaataaat gttgtttact
     7141 atttttttcg tttctgtatt cgcagaacaa gttgacagaa caccctaacg cgttttagtg
     7201 ttaccgggcg gtggcttgtc aagaaatacc ttcccatggc taatacaatt cccaactgga
     7261 agtagtttgg gtttcagaag tcaacagtgt taccgtattt taatgtacat ctggctttta
     7321 agcgagtaca cacacatagc attgcgatgt tttttaaact tgcactgctt gcaagcaggg
     7381 gtccactgta tttcaatgaa agaacagcgt gttgggcgtt tttgaattat actaattacc
     7441 ttcaataaca attccctata tatcacttag ttttaataaa taaatcgttt gagtgtggtc
     7501 tagagatgat ttattatgat gaagtgcggt tgttggggac aagcgggata cgtctgcggg
     7561 tcaaatcggc ggtgggacat agtgagaggg caacggcgaa caatcaccaa cgcgccctgg
     7621 tacgcgagaa atgttgatat cgcacatgac ctcagcatcc accttcagtt tgagaacaaa
     7681 ttaaatcagg cacataccag acaaccagga gaacactgag gaggcgtcat caaagatatc
     7741 tttggacacg tggcataaac aagccaaact attatctaat caatattttt atacaaattt
     7801 tgtatgtttc catgttatct tcgttgctcc tgttagttac tacgtttcat agcccttaaa
     7861 ttggttgctt aacgtaaaat aaaattatat tgtataaaaa taaagaggaa ttcacctata
     7921 acaaacgaaa aagtaaataa ataagaactt ccccatgtta aaaatgccac caccatcttt
     7981 acatataagt atgtacatat gaatgaatca cttaggttgc ttgaatatta caatttcatt
     8041 aatagctaaa tcacatttga tgtgttagtg gaaaacggct atatatataa attatcgaaa
     8101 ttgtgaatat cgaattgcga tagcacaatg ggaaattcca ccactagatt tttggtactt
     8161 ttaacagatc cttttcggtt ttgcgttgcg cgaagtgatc tgaacttatc aaagtttgta
     8221 aggtaataca taaagtgaaa aagaattaat ttgctcttga aaggcaggcc aaattaaaaa
     8281 aaaaatatca atatggacac gtatgccggt gcttacgacc gtcagagccg cgagcgggaa
     8341 aattccagcg cagcaagtcc agccacacag cgtagcgcca acgaggacaa ggcggccgac
     8401 ttgcagcgag aagtcgagcg agatggtggc aggttcaggt tcgtcgggca tttctccgaa
     8461 gctccaggca ctagcgcgtt cggaacagcc gagcgcccgg agttcgaacg catcctgaac
     8521 gagtgccgtg ccggcaggct caacatgatc atagtctatg acgtgagtcg cttctcgcgc
     8581 ctgaaggtca tggacgcgat tccgattgtg agcgaattgc tcgccctggg tgtgacgata
     8641 gtgtccacgc aggaaggcgt gtttcggcag ggaaacgtca tggacctgat acacctgatt
     8701 atgcgcttgg acgcttcgca caaggagtcc agcctgaaga gtgcgaagat tctcgacacc
     8761 aagaacctgc agcgcgaatt gggcggttat gtcggcggta aggctcccta cggcttcgag
     8821 cttgtttcgg agacgaagga gatcacgcgt aacggccgaa tggtcaatgt cgtgatcaac
     8881 aagcttgccc actcgaccac accccttacc cgacccttcg agttcgagcc cgacgttatc
     8941 cgttggtggt ggcgtgagat caagacccac aagcaccttc ccttcaagcc aggcagtcaa
     9001 gccgccattc acccgggcag cattacgggg ctgtgtaagc gcatggacgc agatgctgtg
     9061 ccaacaaggg gtgagacgat tggcaagaag accgcttcaa gcgcctggga ccccgcaacc
     9121 gttatgcgaa tccttcgtga cccgcgtatt gcaggctttg ccgctgaggt gatctacaag
     9181 aagaagccgg atggaacgcc gaccacgaag atagagggtt accgcattca gcgcgatccg
     9241 atcaccttgc gccccgtgga gctcgattgc ggacccatca tcgagcccgc tgagtggtat
     9301 gagctgcagg cgtggttgga tggcaggggt cgcggcaagg gtctttcccg gggacaagcc
     9361 atactgagcg ccatggacaa gctgtactgc gaatgtggcg ccgtgatgac ttcgaagcgc
     9421 ggcgaggaat cgatcaagga ctcctaccgc tgccgtcgac ggaaggtggt tgacccaagc
     9481 gcacctggtc agcatgaggg cacgtgcaac gtgagcatgg cggcactcga caagttcgtt
     9541 gcggaacgca tcttcaacaa gatcaggcac gctgaaggcg acgaagagac gttggccctt
     9601 ctgtgggaag ccgcgcgacg cttcggcaag ctcactgagg cgccagagaa gtccggcgaa
     9661 cgggcgaacc ttgttgcgga gcgagccgat gccttgaacg ccctcgaaga gctgtacgaa
     9721 gaccgcgctg caggagctta cgacggaccc gttggcagga agcacttccg gaagcaacag
     9781 gcagcgctga cactccggca gcaaggagca gaagagcgac ttgccgagct tgaagccgcc
     9841 gaagccccaa agctcccctt ggaccaatgg ttccccgaag acgccgacgc tgacccaact
     9901 ggaccgaagt cgtggtgggg acgcgcatca gtggacgaca agcgcgtgtt tgtgggcctc
     9961 ttcgtggaca agatcgttgt gaccaagagc actaccggca ggggacaggg aacacccatc
    10021 gagaagcgcg cttcgatcac gtgggcaaag cccccgaccg atgacgatga agatgacgcc
    10081 caggatggca ccgaagacgt agcggcctag ctcgagaatg tatggacata gatttcaaat
    10141 aattaaatgt aatgcagtaa ttgatgtaat tagttaaata agttagatat taataacata
    10201 ttaattatat gtattataac gcatataata ataaaatgca tatttaggat atgcaagcca
    10261 ttcgaatttt ctattttaat ttccttttac aaagaaatgt ataacaaaat ataatttgaa
    10321 aaaatgttct ggctctaatt cgatttcttt taagtatttt gtgaactgct tttaataacg
    10381 agcggttgca aaacttaaca gaaacgtgca ctttgatccc aactaatacc gtgtactatc
    10441 acgtgtttgg ttttaagtac tcatccttcg tgtttcgttg tttgttgttt gccttgctgt
    10501 acacttggct cttgcgctct ctcgctctcc gttggagccg gctttttgaa tgcatgcctc
    10561 gccctgctcg cgcccagtcc tcctccatca actttgtata caaaagttga acgagaaacg
    10621 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
    10681 tactgtaaaa cacaacatat ccagtcacta tgggccgcat taggcacccc aggctttaca
    10741 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca
    10801 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
    10861 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
    10921 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
    10981 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
    11041 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
    11101 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
    11161 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
    11221 cctaaagggt ttattgagaa tatgtttttc gtgtcagcca atccctgggt gagtttcacc
    11281 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
    11341 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
    11401 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
    11461 tggcagggcg gggcgtaaac gcgtggcaca tatacctgcc gttcactatt atttagtgaa
    11521 atgagatatt atgatatttt ctgaattgtg attaaaaagg caactttatg cccatgcaac
    11581 agaaactata aaaaatacag agaatgaaaa gaaacagata gattttttag ttctttaggc
    11641 ccgtagtctg caaatccttt tatgattttc tatcaaacaa aagaggaaaa tagaccagtt
    11701 gcaatccaaa cgagagtcta atagaatgag gtcgaaaagt aaatcgcgcg ggtttgttac
    11761 tgataaagca ggcaagacct aaaatgtgta aagggcaaag tgtatacttt ggcgtcaccc
    11821 cttacatatt ttaggtcttt ttttattgtg cgtaactaac ttgccatctt caaacaggag
    11881 ggctggaaga agcagaccgc taacacagta cataaaaaag gagacatgaa cgatgaacat
    11941 caaaaagttt gcaaaacaag caacagtatt aacctttact accgcactgc tggcaggagg
    12001 cgcaactcaa gcgtttgcga aagaaacgaa ccaaaagcca tataaggaaa catacggcat
    12061 ttcccatatt acacgccatg atatgctgca aatccctgaa cagcaaaaaa atgaaaaata
    12121 tcaagttcct gaattcgatt cgtccacaat taaaaatatc tcttctgcaa aaggcctgga
    12181 cgtttgggac agctggccat tacaaaacgc tgacggcact gtcgcaaact atcacggcta
    12241 ccacatcgtc tttgcattag ccggagatcc taaaaatgcg gatgacacat cgatttacat
    12301 gttctatcaa aaagtcggcg aaacttctat tgacagctgg aaaaacgctg gccgcgtctt
    12361 taaagacagc gacaaattcg atgcaaatga ttctatccta aaagaccaaa cacaagaatg
    12421 gtcaggttca gccacattta catctgacgg aaaaatccgt ttattctaca ctgatttctc
    12481 cggtaaacat tacggcaaac aaacactgac aactgcacaa gttaacgtat cagcatcaga
    12541 cagctctttg aacatcaacg gtgtagagga ttataaatca atctttgacg gtgacggaaa
    12601 aacgtatcaa aatgtacagc agttcatcga tgaaggcaac tacagctcag gcgacaacca
    12661 tacgctgaga gatcctcact acgtagaaga taaaggccac aaatacttag tatttgaagc
    12721 aaacactgga actgaagatg gctaccaagg cgaagaatct ttatttaaca aagcatacta
    12781 tggcaaaagc acatcattct tccgtcaaga aagtcaaaaa cttctgcaaa gcgataaaaa
    12841 acgcacggct gagttagcaa acggcgctct cggtatgatt gagctaaacg atgattacac
    12901 actgaaaaaa gtgatgaaac cgctgattgc atctaacaca gtaacagatg aaattgaacg
    12961 cgcgaacgtc tttaaaatga acggcaaatg gtacctgttc actgactccc gcggatcaaa
    13021 aatgacgatt gacggcatta cgtctaacga tatttacatg cttggttatg tttctaattc
    13081 tttaactggc ccatacaagc cgctgaacaa aactggcctt gtgttaaaaa tggatcttga
    13141 tcctaacgat gtaaccttta cttactcaca cttcgctgta cctcaagcga aaggaaacaa
    13201 tgtcgtgatt acaagctata tgacaaacag aggattctac gcagacaaac aatcaacgtt
    13261 tgcgccaagc ttcctgctga acatcaaagg caagaaaaca tctgttgtca aagacagcat
    13321 ccttgaacaa ggacaattaa cagttaacaa ataaatgtca ggctccctta tacacagcca
    13381 gtctgcacgt cgcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
    13441 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact
    13501 ttttaataca aagttgcctc cggaacataa tggtgcatct agatgcccat tcctgtagac
    13561 ttcttaatta agacgtcaga attctcgagg cggccgcatg tgagtctccc tatagtgagt
    13621 cgtattaatt tcgcgggcgc gtcagcggga gagccgttgt aaggcggca
//
