LOCUS       pJAT62{dU6-3-attR3-Amp-ccdB-attR4-vas-phiC31-vasUTR,_ie1-EGFP-attR5-Chlor-SacB-attR6}       14663 bp    DNA     circular UNA 12-JUN-2023
DEFINITION  Ligation of 2 sequences.
ACCESSION   urn.local.sternd.1vz-gdrtpvg
VERSION     urn.local.sternd.1vz-gdrtpvg
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..2273
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P1056
                     p{dU6-3-GW1-Amp-ccdB-vas-phiC31-vasUTR,
                     3XP3-EGFP-GW2-Chlor-SacB} (digested by PspXI, AgeI)
                     Fragment 2 - P859 p{dU6-3-GW1-Amp-ccdB-MCS-GW2-Chlor-SacB}
                     extraction (concatenated sequence 1) (concatenated
                     sequence 1)"
     misc_feature    1..>2273
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P859
                     p{dU6-3-GW1-Amp-ccdB-MCS-GW2-Chlor-SacB} extraction
                     (concatenated sequence 1)"
     CDS             411..1202
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /standard_name="SmR"
     rep_origin      1448..2036
     misc_feature    1820..1837
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ori-252 R"
     primer_bind     1937..1956
                     /note="pBR322 origin, forward primer"
                     /standard_name="pBR322ori-F"
     primer_bind     2026..2045
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7Cas9F"
     primer_bind     complement(2052..2071)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp-ori-7571F"
     misc_feature    2180..2209
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     2190..2207
                     /note="L4440 vector, forward primer"
                     /standard_name="L4440"
     regulatory      2239..2258
                     /note="Geneious type: promoter"
                     /note="SP6 RNA polymerase promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SP6"
     misc_feature    2270..6028
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P776
                     p{dU6-3-GW1-Amp-ccdB-ie1-GFP-FRT-Mhc-dsRed-GW2-Chlor-SacB}
                     (digested by PspXI, AgeI) Fragment 1 (concatenated
                     sequence 2)"
     misc_feature    2270..2273
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2300..2303
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     2304..2322
                     /standard_name="1 F"
     regulatory      2334..2754
                     /note="Geneious type: promoter"
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
                     /standard_name="dU6-3 promoter"
     misc_feature    2758..2787
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2761..2884
                     /note="Geneious type: attR3"
                     /standard_name="attR3"
     primer_bind     complement(2958..2979)
                     /standard_name="676 R"
     misc_feature    2976..2979
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      2980..3084
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     primer_bind     2980..2999
                     /standard_name="1 F"
     misc_feature    3085..3945
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     primer_bind     complement(3125..3146)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AMP_5'_F"
     misc_feature    3331..3350
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_R"
     misc_feature    3804
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="T"
     primer_bind     complement(3817..3836)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_F"
     misc_feature    3909..3932
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp_3'_R"
     primer_bind     complement(3922..3945)
                     /standard_name="966 R"
     misc_feature    3942..3945
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    3946..4585
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGT
                     TGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAAT
                     GAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
                     AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTG
                     AAATGCAATTTAAGGTTTATACTTACAAGCGCGAGTCCCGCTATCGCCTTTTCGTGGA
                     CGTCCAGTCAGACATTATCGACACGCCTGGTCGCCGTATGGTGATTCCATTAGCCAGC
                     GCACGCCTGTTAAGTGATAAGGTGTCGCGCGAGCTTTACCCAGTCGTCCATATTGGTG
                     ACGAGAGTTGGCGCATGATGACTACAGACATGGCTAGTGTACCTGTGTCGGTGATCGG
                     GGAAGAGGTCGCGGATTTAAGCCACCGTGAGAATGATATTAAGAATGCAATCAATCTC
                     ATGTTTTGGGGTATTTA"
     primer_bind     3946..3967
                     /standard_name="1 F"
     gene            4255..4587
                     /standard_name="ccdB"
     misc_feature    complement(4625..4748)
                     /note="Geneious type: attR4"
                     /standard_name="attR4"
     misc_feature    4752..4755
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    4756..5855
                     /locus_tag="ie1 promoter"
                     /note="Geneious type: Enhancer"
                     /standard_name="ie1"
     primer_bind     4756..4772
                     /standard_name="1 F"
     primer_bind     complement(4770..4790)
                     /standard_name="ie1-HDR-4,790 R"
     regulatory      4773..5255
                     /note="Geneious type: enhancer"
                     /note="Autographa californica hr5 enhancer"
                     /regulatory_class="enhancer"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="hr5"
     regulatory      5259..5850
                     /note="Geneious type: promoter"
                     /note="Autographa californica nucleopolyhedrovirus IE-1
                     promoter region"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="IE-1"
     misc_feature    complement(5762..5784)
                     /note="Geneious type: CRISPR"
                     /standard_name="CRISPR guide 15"
     misc_feature    5773..5795
                     /note="Geneious type: CRISPR"
                     /standard_name="CRISPR guide 1"
     misc_feature    5802..5824
                     /note="Geneious type: CRISPR"
                     /standard_name="CRISPR guide 2"
     misc_feature    5805..5827
                     /note="Geneious type: CRISPR"
                     /standard_name="CRISPR guide 3"
     misc_feature    complement(5846..5868)
                     /note="Geneious type: CRISPR"
                     /standard_name="CRISPR guide 14"
     misc_feature    5852..5874
                     /standard_name="specific CRISPR site G"
     misc_feature    5852..5874
                     /note="Geneious type: CRISPR"
                     /standard_name="CRISPR guide 4"
     misc_feature    complement(5854..5876)
                     /note="Geneious type: CRISPR"
                     /standard_name="CRISPR guide 13"
     misc_feature    complement(5861..5883)
                     /note="Geneious type: CRISPR"
                     /standard_name="CRISPR guide 12"
     misc_feature    complement(5862..5884)
                     /note="Geneious type: CRISPR"
                     /standard_name="CRISPR guide 11"
     misc_feature    complement(5863..5882)
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="4558 R"
     misc_feature    5866..5888
                     /note="Geneious type: CRISPR"
                     /standard_name="CRISPR guide 5"
     misc_feature    complement(5868..5890)
                     /note="Geneious type: CRISPR"
                     /standard_name="CRISPR guide 10"
     regulatory      5869..>6028
                     /note="Geneious type: promoter"
                     /standard_name="HS promoter"
     misc_feature    complement(5871..5893)
                     /note="Geneious type: CRISPR"
                     /standard_name="CRISPR guide 9"
     misc_feature    5880..5902
                     /note="Geneious type: CRISPR"
                     /standard_name="CRISPR guide 6"
     misc_feature    complement(5907..5925)
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="12878 R"
     misc_feature    complement(5907..5925)
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2020.2.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="P693
                     p(MHC-DsRed-CDNopt-5'myr-smGFP-int-B3-B3 recombinase-FSF)
                     - 12878 R (reversed)"
     misc_feature    5973..5995
                     /note="Geneious type: CRISPR"
                     /standard_name="CRISPR guide 7"
     misc_feature    5974..5996
                     /note="Geneious type: CRISPR"
                     /standard_name="CRISPR guide 8"
     misc_feature    6025..6028
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    6025..14663
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P1056
                     p{dU6-3-GW1-Amp-ccdB-vas-phiC31-vasUTR,
                     3XP3-EGFP-GW2-Chlor-SacB} (digested by PspXI, AgeI)
                     Fragment 2 extraction (concatenated sequence 3)"
     misc_feature    <6025..11582
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P53 pBac{vas-phiC31-vasUTR, 3XP3-EGFP} PCR
                     Product (concatenated sequence 2)"
     regulatory      <6025..6036
                     /note="Geneious type: promoter"
                     /note="Derived using Geneious Prime 2020.2.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="HS promoter"
     misc_feature    6037..6753
                     /note="Geneious type: Reporter"
                     /standard_name="EGFP"
     regulatory      6772..7016
                     /note="Geneious type: Terminator"
                     /standard_name="SV40_PA term"
     regulatory      7017..9286
                     /note="Geneious type: promoter"
                     /standard_name="vas promoter"
     CDS             9287..11104
                     /standard_name="phiC31"
     3'UTR           11111..11258
                     /standard_name="vas 3'UTR"
     misc_feature    11111..11578
                     /standard_name="2L 3' vas"
     primer_bind     complement(11563..11578)
                     /standard_name="5,825 R"
     misc_feature    complement(11579..11582)
                     /note="4 base BsaI overhang created during Golden Gate
                     assembly (BsaI site 395-400)"
                     /standard_name="BsaI overhang"
     misc_feature    <11579..11586
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pGGAselect-Spect extraction (concatenated
                     sequence 2)"
     misc_feature    <11579..>11582
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pGGAselect PCR Product (concatenated
                     sequence 2)"
     misc_feature    11579..14663
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P859
                     p{dU6-3-GW1-Amp-ccdB-MCS-GW2-Chlor-SacB} extraction
                     (concatenated sequence 3)"
     misc_feature    11579..11582
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    11583..11706
                     /note="Geneious type: attR5"
                     /standard_name="attR5"
     misc_feature    11583..>2273
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P767
                     p{dU6-3-GW1-Amp-ccdB-Mhc-GFP-FRT-P3-DsRed-GW2-Chlor-SacB}
                     (concatenated sequence 1)"
     misc_feature    11583..11586
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     gene            11813..12472
                     /gene="CAT/CamR"
                     /standard_name="CAT/CamR"
     primer_bind     complement(11911..11930)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="10,588 R"
     misc_feature    12477..12480
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      12481..12926
                     /note="Geneious type: promoter"
                     /note="Bacillus subtilis sacB (glycoside hydrolase family
                     68) promoter region"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB"
     gene            12927..14350
                     /note="counterselectable marker from Bacillus subtilis
                     that converts sucrose to levans"
                     /product="levansucrase"
                     /gene="sacB"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB CS"
     misc_feature    13318..13321
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    complement(14387..14510)
                     /note="Geneious type: attR6"
                     /standard_name="attR6"
     misc_feature    14511..14540
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    14541..14544
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      complement(14603..14621)
                     /note="Geneious type: Promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7 prom"
     regulatory      complement(14604..14621)
                     /regulatory_class="promoter"
                     /note="T7 promoter"
                     /standard_name="T7 promoter"
     misc_feature    14634..14663
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
ORIGIN      
        1 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
       61 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
      121 tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
      181 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
      241 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
      301 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      361 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
      421 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
      481 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
      541 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
      601 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
      661 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
      721 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
      781 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
      841 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
      901 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
      961 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     1021 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1081 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     1141 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     1201 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     1261 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     1321 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca
     1381 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1441 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1501 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1561 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1621 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1681 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1741 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1801 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1861 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1921 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1981 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2041 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2101 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2161 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa atcgtcacct
     2221 gcaggaaggt ttaaacgcat ttaggtgaca ctatagaagt gtgtatcgct cgagggatcc
     2281 gaattcgaag tcttggtacg gaggggatcc gatcgacaag ccgaacatat gggttttttg
     2341 ctcacctgtg attgctccta ctcaaataca aaaacatcaa attttctgtc aataaagcat
     2401 atttatttat atttatttta caggaaagaa ttccttttaa agtgtatttt aacctataat
     2461 gaaaaacgat taaaaaaaat acataaaata attcgaaaat ttttgaatag cccaggttga
     2521 taaaaattca tttcatacgt tttataactt atgcccctaa gtattttttg accatagtgt
     2581 ttcaattcta cattaatttt acagagtaga atgaaacgcc acctactcag ccaagaggcg
     2641 aaaaggttag ctcgccaagc agagagggcg ccagtgctca ctacttttta taattctcaa
     2701 cttctttttc cagactcagt tcgtatatat agacctattt tcaatttaac gtcggggcct
     2761 caactttgta taataaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     2821 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     2881 tatgggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
     2941 ggattttgag ttaggatccg tcgagatttt caggagctac gcggaacccc tatttgttta
     3001 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3061 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3121 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3181 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3241 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3301 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3361 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3421 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3481 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3541 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3601 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3661 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3721 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3781 tctggagccg gtgagcgtgg gtcccgcggt atcattgcag cactggggcc agatggtaag
     3841 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3901 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaatggat ccggcttact
     3961 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     4021 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     4081 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     4141 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     4201 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     4261 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     4321 tatcgtctgt ttgtggatgt acagagtgat attatcgata caccgggccg tcgcatggtt
     4381 attccacttg cgtctgcgcg cttgctctca gataaagtct cccgtgaact ttacccggtg
     4441 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtt
     4501 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     4561 attaacctga tgttctgggg aatataatgt caggctccct tatacacagc cagtctgcac
     4621 gtcgcatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     4681 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa cttttctata
     4741 caaagttgtc cagctccgag ctcttacgcg cccgcgtaaa acacaatcaa gtatgagtca
     4801 taagctgatg tcatgttttg cacacggctc ataaccgaac tggctttacg agtagaattc
     4861 tacttgtaac gcacgatcag tggatgatgt catttgtttt tcaaatcgag atgatgtcat
     4921 gttttgcaca cggctcataa actcgcttta cgagtagaat tctacgtgta acgcacgatc
     4981 gattgatgag tcatttgttt tgcaatatga tatcatacaa tatgactcat ttgtttttca
     5041 aaaccgaact tgatttacgg gtagaattct acttgtaaag cacaatcaaa aagatgatgt
     5101 catttgtttt tcaaaactga actcgcttta cgagtagaat tctacgtgta aaacacaatc
     5161 aagaaatgat gtcatttgtt ataaaaataa aagctgatgt catgttttgc acatggctca
     5221 taactaaact cgctttacgg gtagaattct acgcgcgtcg atgtctttgt gatgcgcgcg
     5281 acatttttgt aggttattga taaaatgaac ggatacgttg cccgacatta tcattaaatc
     5341 cttggcgtag aatttgtcgg gtccattgtc cgtgtgcgct agcatgcccg taacggacct
     5401 cgtacttttg gcttcaaagg ttttgcgcac agacaaaatg tgccacactt gcagctctgc
     5461 atgtgtgcgc gttaccacaa atcccaacgg cgcagtgtac ttgttgtatg caaataaatc
     5521 tcgataaagg cgcggcgcgc gaatgcagct gatcacgtac gctcctcgtg ttccgttcaa
     5581 ggacggtgtt atcgacctca gattaatgtt tatcggccga ctgttttcgt atccgctcac
     5641 caaacgcgtt tttgcattaa cattgtatgt cggcggatgt tctatatcta atttgaataa
     5701 ataaacgata accgcgttgg ttttagaggg cataataaaa gaaatattgt tatcgtgttc
     5761 gccattaggg cagtataaat tgacgttcat gttggatatt gtttcagttg caagttgaca
     5821 ctggcggcga caagatcgtg aacaaccaag tgaccattaa ccctcgaccg ccggagtata
     5881 aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc
     5941 gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca atcggggtac
     6001 cgctagagtc gacggtacga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg
     6061 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     6121 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     6181 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     6241 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     6301 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     6361 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     6421 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     6481 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     6541 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     6601 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     6661 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     6721 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa
     6781 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     6841 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     6901 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6961 attctagttg tggtttgtcc aaactcatca atgtatctta aagcttatcg atacgcgtac
     7021 ggcgcgcgag gtcgacggta tcgataagct tgatatcgaa ttcctgcagc tggttgtagg
     7081 tgcagttgcg cttcttgcac ttggcgcact tgaggagatc ggtcttggtg ccctgcacgg
     7141 tggccagctg ggcatcgttg atggcctcct tgacgaactt ctcgcgcagc ttcttcatct
     7201 cgtcgctggc catctcctcc ggcgtcattt tggccagctg cttggcagtg acggcgccgc
     7261 acataaagtt gccgcgcaat ccaggattct tggggtcctt cagattggcc acgcgtgacc
     7321 taatgcgatt cttgtacttc atatccgtgt tattgaactc ggagtaaatg gcatcctcca
     7381 gttcggcggc catttcctcc ggctcaccgc atccctcggg cacttcgcca atcttcagtg
     7441 cggtggccag catttcgcgg catttgatgc ggaccgcatc tgtcatgcca ccggagggaa
     7501 acgaggtctg tgacgaggat gtcgagcctt tcttctcttt atccttggat gagctggatg
     7561 aactggactt atccttgccg gagattgacg acgacgactt ggcggcactg gtggacttcg
     7621 aggcactgct gttgttggag gagccctcct tagcggagct gttgttcggt gtggttggcg
     7681 ccgggctggc caggaagcgt ttccagttct tgatcagcgt cttggccaga gcgatcacct
     7741 cgtcgtcctt gctactcttg cgcagctcgt ttacggtcat gccgatgcgc gttttggtca
     7801 gaatgtcgag attgatgtta agcgtttgca gggccttcag caggtccaga gcctgatcct
     7861 gtccctgtac ggtggatata tgtatatttg tgacatgatg cgcggcacgt tagctaatcc
     7921 cgatacttac tgtgccgtcg ctggccatct tgctcatctt cttttggatt cgaaacactt
     7981 cctcctccac gctcattttg gcccacttaa tttgatcaga ggttaggtta actggcgtcg
     8041 gctgtcggct ttaatcggtg ctgcgggtct gcgacagttg tttatgcgga agccgggcta
     8101 tttctttttt tccctccaat aaatgttgtt tactattttt ttcgtttctg tattcgcaga
     8161 acaagttgac agaacaccct aacgcgtttt agtgttaccg ggcggtggct tgtcaagaaa
     8221 taccttccca tggctaatac aattcccaac tggaagtagt ttgggtttca gaagtcaaca
     8281 gtgttaccgt attttaatgt acatctggct tttaagcgag tacacacaca tagcattgcg
     8341 atgtttttta aacttgcact gcttgcaagc aggggtccac tgtatttcaa tgaaagaaca
     8401 gcgtgttggg cgtttttgaa ttatactaat taccttcaat aacaattccc tatatatcac
     8461 ttagttttaa taaataaatc gtttgagtgt ggtctagaga tgatttatta tgatgaagtg
     8521 cggttgttgg ggacaagcgg gatacgtctg cgggtcaaat cggcggtggg acatagtgag
     8581 agggcaacgg cgaacaatca ccaacgcgcc ctggtacgcg agaaatgttg atatcgcaca
     8641 tgacctcagc atccaccttc agtttgagaa caaattaaat caggcacata ccagacaacc
     8701 aggagaacac tgaggaggcg tcatcaaaga tatctttgga cacgtggcat aaacaagcca
     8761 aactattatc taatcaatat ttttatacaa attttgtatg tttccatgtt atcttcgttg
     8821 ctcctgttag ttactacgtt tcatagccct taaattggtt gcttaacgta aaataaaatt
     8881 atattgtata aaaataaaga ggaattcacc tataacaaac gaaaaagtaa ataaataaga
     8941 acttccccat gttaaaaatg ccaccaccat ctttacatat aagtatgtac atatgaatga
     9001 atcacttagg ttgcttgaat attacaattt cattaatagc taaatcacat ttgatgtgtt
     9061 agtggaaaac ggctatatat ataaattatc gaaattgtga atatcgaatt gcgatagcac
     9121 aatgggaaat tccaccacta gatttttggt acttttaaca gatccttttc ggttttgcgt
     9181 tgcgcgaagt gatctgaact tatcaaagtt tgtaaggtaa tacataaagt gaaaaagaat
     9241 taatttgctc ttgaaaggca ggccaaatta aaaaaaaaat atcaatatgg acacgtatgc
     9301 cggtgcttac gaccgtcaga gccgcgagcg ggaaaattcc agcgcagcaa gtccagccac
     9361 acagcgtagc gccaacgagg acaaggcggc cgacttgcag cgagaagtcg agcgagatgg
     9421 tggcaggttc aggttcgtcg ggcatttctc cgaagctcca ggcactagcg cgttcggaac
     9481 agccgagcgc ccggagttcg aacgcatcct gaacgagtgc cgtgccggca ggctcaacat
     9541 gatcatagtc tatgacgtga gtcgcttctc gcgcctgaag gtcatggacg cgattccgat
     9601 tgtgagcgaa ttgctcgccc tgggtgtgac gatagtgtcc acgcaggaag gcgtgtttcg
     9661 gcagggaaac gtcatggacc tgatacacct gattatgcgc ttggacgctt cgcacaagga
     9721 gtccagcctg aagagtgcga agattctcga caccaagaac ctgcagcgcg aattgggcgg
     9781 ttatgtcggc ggtaaggctc cctacggctt cgagcttgtt tcggagacga aggagatcac
     9841 gcgtaacggc cgaatggtca atgtcgtgat caacaagctt gcccactcga ccacacccct
     9901 tacccgaccc ttcgagttcg agcccgacgt tatccgttgg tggtggcgtg agatcaagac
     9961 ccacaagcac cttcccttca agccaggcag tcaagccgcc attcacccgg gcagcattac
    10021 ggggctgtgt aagcgcatgg acgcagatgc tgtgccaaca aggggtgaga cgattggcaa
    10081 gaagaccgct tcaagcgcct gggaccccgc aaccgttatg cgaatccttc gtgacccgcg
    10141 tattgcaggc tttgccgctg aggtgatcta caagaagaag ccggatggaa cgccgaccac
    10201 gaagatagag ggttaccgca ttcagcgcga tccgatcacc ttgcgccccg tggagctcga
    10261 ttgcggaccc atcatcgagc ccgctgagtg gtatgagctg caggcgtggt tggatggcag
    10321 gggtcgcggc aagggtcttt cccggggaca agccatactg agcgccatgg acaagctgta
    10381 ctgcgaatgt ggcgccgtga tgacttcgaa gcgcggcgag gaatcgatca aggactccta
    10441 ccgctgccgt cgacggaagg tggttgaccc aagcgcacct ggtcagcatg agggcacgtg
    10501 caacgtgagc atggcggcac tcgacaagtt cgttgcggaa cgcatcttca acaagatcag
    10561 gcacgctgaa ggcgacgaag agacgttggc ccttctgtgg gaagccgcgc gacgcttcgg
    10621 caagctcact gaggcgccag agaagtccgg cgaacgggcg aaccttgttg cggagcgagc
    10681 cgatgccttg aacgccctcg aagagctgta cgaagaccgc gctgcaggag cttacgacgg
    10741 acccgttggc aggaagcact tccggaagca acaggcagcg ctgacactcc ggcagcaagg
    10801 agcagaagag cgacttgccg agcttgaagc cgccgaagcc ccaaagctcc ccttggacca
    10861 atggttcccc gaagacgccg acgctgaccc aactggaccg aagtcgtggt ggggacgcgc
    10921 atcagtggac gacaagcgcg tgtttgtggg cctcttcgtg gacaagatcg ttgtgaccaa
    10981 gagcactacc ggcaggggac agggaacacc catcgagaag cgcgcttcga tcacgtgggc
    11041 aaagcccccg accgatgacg atgaagatga cgcccaggat ggcaccgaag acgtagcggc
    11101 ctagctcgag aatgtatgga catagatttc aaataattaa atgtaatgca gtaattgatg
    11161 taattagtta aataagttag atattaataa catattaatt atatgtatta taacgcatat
    11221 aataataaaa tgcatattta ggatatgcaa gccattcgaa ttttctattt taatttcctt
    11281 ttacaaagaa atgtataaca aaatataatt tgaaaaaatg ttctggctct aattcgattt
    11341 cttttaagta ttttgtgaac tgcttttaat aacgagcggt tgcaaaactt aacagaaacg
    11401 tgcactttga tcccaactaa taccgtgtac tatcacgtgt ttggttttaa gtactcatcc
    11461 ttcgtgtttc gttgtttgtt gtttgccttg ctgtacactt ggctcttgcg ctctctcgct
    11521 ctccgttgga gccggctttt tgaatgcatg cctcgccctg ctcgcgccca gtcctcctcc
    11581 atcaactttg tatacaaaag ttgaacgaga aacgtaaaat gatataaata tcaatatatt
    11641 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
    11701 actatgggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
    11761 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
    11821 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
    11881 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
    11941 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
    12001 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
    12061 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
    12121 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
    12181 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
    12241 tttcgtgtca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
    12301 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
    12361 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
    12421 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg
    12481 cacatatacc tgccgttcac tattatttag tgaaatgaga tattatgata ttttctgaat
    12541 tgtgattaaa aaggcaactt tatgcccatg caacagaaac tataaaaaat acagagaatg
    12601 aaaagaaaca gatagatttt ttagttcttt aggcccgtag tctgcaaatc cttttatgat
    12661 tttctatcaa acaaaagagg aaaatagacc agttgcaatc caaacgagag tctaatagaa
    12721 tgaggtcgaa aagtaaatcg cgcgggtttg ttactgataa agcaggcaag acctaaaatg
    12781 tgtaaagggc aaagtgtata ctttggcgtc accccttaca tattttaggt ctttttttat
    12841 tgtgcgtaac taacttgcca tcttcaaaca ggagggctgg aagaagcaga ccgctaacac
    12901 agtacataaa aaaggagaca tgaacgatga acatcaaaaa gtttgcaaaa caagcaacag
    12961 tattaacctt tactaccgca ctgctggcag gaggcgcaac tcaagcgttt gcgaaagaaa
    13021 cgaaccaaaa gccatataag gaaacatacg gcatttccca tattacacgc catgatatgc
    13081 tgcaaatccc tgaacagcaa aaaaatgaaa aatatcaagt tcctgaattc gattcgtcca
    13141 caattaaaaa tatctcttct gcaaaaggcc tggacgtttg ggacagctgg ccattacaaa
    13201 acgctgacgg cactgtcgca aactatcacg gctaccacat cgtctttgca ttagccggag
    13261 atcctaaaaa tgcggatgac acatcgattt acatgttcta tcaaaaagtc ggcgaaactt
    13321 ctattgacag ctggaaaaac gctggccgcg tctttaaaga cagcgacaaa ttcgatgcaa
    13381 atgattctat cctaaaagac caaacacaag aatggtcagg ttcagccaca tttacatctg
    13441 acggaaaaat ccgtttattc tacactgatt tctccggtaa acattacggc aaacaaacac
    13501 tgacaactgc acaagttaac gtatcagcat cagacagctc tttgaacatc aacggtgtag
    13561 aggattataa atcaatcttt gacggtgacg gaaaaacgta tcaaaatgta cagcagttca
    13621 tcgatgaagg caactacagc tcaggcgaca accatacgct gagagatcct cactacgtag
    13681 aagataaagg ccacaaatac ttagtatttg aagcaaacac tggaactgaa gatggctacc
    13741 aaggcgaaga atctttattt aacaaagcat actatggcaa aagcacatca ttcttccgtc
    13801 aagaaagtca aaaacttctg caaagcgata aaaaacgcac ggctgagtta gcaaacggcg
    13861 ctctcggtat gattgagcta aacgatgatt acacactgaa aaaagtgatg aaaccgctga
    13921 ttgcatctaa cacagtaaca gatgaaattg aacgcgcgaa cgtctttaaa atgaacggca
    13981 aatggtacct gttcactgac tcccgcggat caaaaatgac gattgacggc attacgtcta
    14041 acgatattta catgcttggt tatgtttcta attctttaac tggcccatac aagccgctga
    14101 acaaaactgg ccttgtgtta aaaatggatc ttgatcctaa cgatgtaacc tttacttact
    14161 cacacttcgc tgtacctcaa gcgaaaggaa acaatgtcgt gattacaagc tatatgacaa
    14221 acagaggatt ctacgcagac aaacaatcaa cgtttgcgcc aagcttcctg ctgaacatca
    14281 aaggcaagaa aacatctgtt gtcaaagaca gcatccttga acaaggacaa ttaacagtta
    14341 acaaataaat gtcaggctcc cttatacaca gccagtctgc acgtcgcata gtgactggat
    14401 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
    14461 gatatttata tcattttacg tttctcgttc aactttttaa tacaaagttg cctccggaac
    14521 ataatggtgc atctagatgc ccattcctgt agacttctta attaagacgt cagaattctc
    14581 gaggcggccg catgtgagtc tccctatagt gagtcgtatt aatttcgcgg gcgcgtcagc
    14641 gggagagccg ttgtaaggcg gca
//
