LOCUS       pJAT71{dU6-3-attR3-Amp-ccdB-attR4-20XUAS-IVS-Syn21-op1-jGCaMP7s-T2A-tdTomato-p10.536.1473-B3-ie1-dsRed-B3-attR5-Chlor-SacB-attR6}       14652 bp    DNA     circular UNA 12-JUN-2023
DEFINITION  Generated by Gibson/Homology Cloning (P1066
            p{dU6-3-GW1-Amp-ccdB-20XUAS-IVS-Syn21-op1-jGCaMP7s-T2A-tdTomato-p10
            .536.1473- B3-3xP3::dsRed-B3-GW2-Chlor-SacB} (digested by SgsI)
            Fragment 2, P862-ie1-DsRed (reversed)).
ACCESSION   urn.local.sternd.206-gdrtyzc
VERSION     urn.local.sternd.206-gdrtyzc
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             411..1202
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /standard_name="SmR"
     rep_origin      1448..2036
     regulatory      2239..2258
                     /note="Geneious type: promoter"
                     /note="SP6 RNA polymerase promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SP6"
     regulatory      2334..2754
                     /note="Geneious type: promoter"
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
                     /standard_name="dU6-3 promoter"
     misc_feature    2761..2884
                     /note="Geneious type: attR3"
                     /standard_name="attR3"
     regulatory      2980..3084
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     gene            3085..3945
                     /standard_name="AmpR"
     gene            4255..4587
                     /standard_name="ccdB"
     misc_feature    complement(4625..4748)
                     /note="Geneious type: attR4"
                     /standard_name="attR4"
     misc_feature    4887..4903
                     /standard_name="UAS"
     misc_feature    4906..4922
                     /standard_name="UAS"
     misc_feature    4925..4941
                     /standard_name="UAS"
     misc_feature    4944..4960
                     /standard_name="UAS"
     misc_feature    4963..4979
                     /standard_name="UAS"
     misc_feature    5015..5031
                     /standard_name="UAS"
     misc_feature    5034..5050
                     /standard_name="UAS"
     misc_feature    5053..5069
                     /standard_name="UAS"
     misc_feature    5072..5088
                     /standard_name="UAS"
     misc_feature    5091..5107
                     /standard_name="UAS"
     misc_feature    5143..5159
                     /standard_name="UAS"
     misc_feature    5162..5178
                     /standard_name="UAS"
     misc_feature    5181..5197
                     /standard_name="UAS"
     misc_feature    5200..5216
                     /standard_name="UAS"
     misc_feature    5219..5235
                     /standard_name="UAS"
     misc_feature    5271..5287
                     /standard_name="UAS"
     misc_feature    5290..5306
                     /standard_name="UAS"
     misc_feature    5309..5325
                     /standard_name="UAS"
     misc_feature    5328..5344
                     /standard_name="UAS"
     misc_feature    5347..5363
                     /standard_name="UAS"
     regulatory      5388..5615
                     /note="Geneious type: promoter"
                     /standard_name="HS_promoter"
     misc_feature    5643..5708
                     /standard_name="Myosin heavy chain intron (IVS)"
     misc_feature    5718..5738
                     /note="Geneious type: Leader"
                     /standard_name="Syn21"
     CDS             5739..>7088
                     /standard_name="jGCaMP7s codon opt"
     CDS             7089..7142
                     /standard_name="T2A"
     CDS             <7143..8570
                     /standard_name="tdTomato (Dm codon optimized)"
     gene            8587..9258
                     /note="p10 fragment"
                     /note="Geneious type: 3'UTR"
                     /standard_name="p10"
     misc_feature    9370..9402
                     /note="Geneious type: Recombination site"
                     /standard_name="B3"
     regulatory      complement(9543..9787)
                     /note="Geneious type: terminator"
                     /standard_name="SV40_PA term"
     gene            complement(9789..10468)
                     /standard_name="DsRed2 reporter"
     regulatory      complement(10469..>10470)
                     /note="Geneious type: Promoter"
                     /standard_name="HS prom"
     regulatory      complement(10471..10638)
                     /note="Geneious type: promoter"
                     /standard_name="HS promoter"
     regulatory      complement(10657..11248)
                     /note="Geneious type: promoter"
                     /note="Autographa californica nucleopolyhedrovirus IE-1
                     promoter region"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="IE-1"
     regulatory      11252..11451
                     /note="Geneious type: enhancer"
                     /note="Autographa californica hr5 enhancer"
                     /regulatory_class="enhancer"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="hr5"
     misc_feature    11293^11294
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="TGTGCAAAACATGACATCAGCTTTTATTTTTATAACAAATGA
                     CATCATTTCTTGATTGTGTTTTACACGTAGAATTCTACTCGTAAAGCGAGTTCAGTTT
                     TGAAAAACAAATGACATCATCTTTTTGATTGTGCTTTACAAGTAGAATTCTACCCGTA
                     AATCAAGTTCGGTTTTGAAAAACAAATGAGTCATATTGTATGATATCATATTGCAAAA
                     CAAATGACTCATCAATCGATCGTGCGTTACACGTAGAATTCTACTCGTAAAGCGAGTT
                     TATGAGCCG"
     misc_feature    11530..11562
                     /note="Geneious type: Recombination site"
                     /standard_name="B3"
     misc_feature    11572..11695
                     /note="Geneious type: attR5"
                     /standard_name="attR5"
     gene            11802..12461
                     /gene="CAT/CamR"
                     /standard_name="CAT/CamR"
     regulatory      12470..12915
                     /note="Geneious type: promoter"
                     /note="Bacillus subtilis sacB (glycoside hydrolase family
                     68) promoter region"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB"
     gene            12916..14339
                     /note="counterselectable marker from Bacillus subtilis
                     that converts sucrose to levans"
                     /product="levansucrase"
                     /gene="sacB"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB CS"
     misc_feature    complement(14376..14499)
                     /note="Geneious type: attR6"
                     /standard_name="attR6"
     regulatory      complement(14593..14610)
                     /regulatory_class="promoter"
                     /note="T7 promoter"
                     /standard_name="T7 promoter"
ORIGIN      
        1 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
       61 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
      121 tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
      181 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
      241 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
      301 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      361 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
      421 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
      481 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
      541 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
      601 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
      661 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
      721 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
      781 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
      841 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
      901 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
      961 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     1021 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1081 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     1141 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     1201 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     1261 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     1321 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca
     1381 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1441 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1501 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1561 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1621 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1681 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1741 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1801 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1861 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1921 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1981 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2041 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2101 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2161 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa atcgtcacct
     2221 gcaggaaggt ttaaacgcat ttaggtgaca ctatagaagt gtgtatcgct cgagggatcc
     2281 gaattcgaag tcttggtacg gaggggatcc gatcgacaag ccgaacatat gggttttttg
     2341 ctcacctgtg attgctccta ctcaaataca aaaacatcaa attttctgtc aataaagcat
     2401 atttatttat atttatttta caggaaagaa ttccttttaa agtgtatttt aacctataat
     2461 gaaaaacgat taaaaaaaat acataaaata attcgaaaat ttttgaatag cccaggttga
     2521 taaaaattca tttcatacgt tttataactt atgcccctaa gtattttttg accatagtgt
     2581 ttcaattcta cattaatttt acagagtaga atgaaacgcc acctactcag ccaagaggcg
     2641 aaaaggttag ctcgccaagc agagagggcg ccagtgctca ctacttttta taattctcaa
     2701 cttctttttc cagactcagt tcgtatatat agacctattt tcaatttaac gtcggggcct
     2761 caactttgta taataaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     2821 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     2881 tatgggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
     2941 ggattttgag ttaggatccg tcgagatttt caggagctac gcggaacccc tatttgttta
     3001 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3061 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3121 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3181 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3241 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3301 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3361 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3421 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3481 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3541 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3601 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3661 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3721 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3781 tctggagccg gtgagcgtgg gtcccgcggt atcattgcag cactggggcc agatggtaag
     3841 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3901 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaatggat ccggcttact
     3961 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     4021 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     4081 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     4141 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     4201 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     4261 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     4321 tatcgtctgt ttgtggatgt acagagtgat attatcgata caccgggccg tcgcatggtt
     4381 attccacttg cgtctgcgcg cttgctctca gataaagtct cccgtgaact ttacccggtg
     4441 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtt
     4501 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     4561 attaacctga tgttctgggg aatataatgt caggctccct tatacacagc cagtctgcac
     4621 gtcgcatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     4681 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa cttttctata
     4741 caaagttgtc cagctggagg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa
     4801 ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag
     4861 ggccgcaagc ttgcatgcct gcaggtcgga gtactgtcct ccgagcggag tactgtcctc
     4921 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga
     4981 gcggagactc tagccctagg gcatgcctgc aggtcggagt actgtcctcc gagcggagta
     5041 ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg
     5101 tcctccgagc ggagactcta gcgctagcgc atgcctgcag gtcggagtac tgtcctccga
     5161 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
     5221 gagtactgtc ctccgagcgg agactctagc actagtgcat gcctgcaggt cggagtactg
     5281 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
     5341 tccgagcgga gtactgtcct ccgagcggag actctagcga cgtcgagcgc cggagtataa
     5401 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg
     5461 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa
     5521 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact
     5581 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagatctaaa
     5641 aggtaggttc aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct
     5701 ttacttcagg cggccgcaac ttaaaaaaaa aaatcaaaat gggctcacat catcaccacc
     5761 accacggaat ggctagcatg acgggcggac aacagatggg ccgcgatctg tacgatgatg
     5821 atgataagga cctggcgaca atggttgaca gcagccggcg caagtggaac aaaacaggac
     5881 atgcagtcag agtcattggc cgcctttcct cactggaaaa cgtctacata aaggcggata
     5941 agcagaagaa cggtatcaaa gccaatttcc atatccgcca caacatcgaa gatggcggag
     6001 tgcagctcgc ataccactac caacagaata cgccaatcgg agacggcccc gtgctgctgc
     6061 ccgacaacca ttacctgagt gttcagtcga agcttagcaa ggacccaaac gaaaaacgag
     6121 atcatatggt tctgctggaa tttgtgactg ctgctggtat taccctgggc atggatgaac
     6181 tctataaggg tggaacggga ggctccatgg ttagcaaagg cgaggagctg ttcactggcg
     6241 tcgtgccgat cctggtcgag ttggacggag atgttaatgg acacaaattc agtgtgagtg
     6301 gcgagggcga gggcgatgcg acatacggca agttgacctt gaagttcatt tgcaccaccg
     6361 gtaaactgcc agtaccctgg cccacgctgg tcaccacgct cacctatggc gtccagtgct
     6421 tctctcgtta tccggatcac atgaagcagc atgatttctt caagtccgcc atgcctgagg
     6481 ggtacattca agagcgaaca atcttcttca aggacgacgg caattacaag accagagccg
     6541 aagtgaagtt tgaaggcgat acactagtga accgcatcga gctaaagggt attgatttta
     6601 aggaggatgg caacatcttg ggccacaagc tggagtacaa ccttcccgat cagctaaccg
     6661 aggagcagat cgcggagttc aaggaactgt tttcgctgtt cgacaaagat ggtgatggta
     6721 cgattaccac gaaggaattg ggaacagtga tgcggtccct ggggcagaac cccaccgagg
     6781 ccgagctgca ggatatgata aacgaggtgg atgctgatgg tgacggcacg attgattttc
     6841 ccgagttctt gaccatgatg gcccgaaaga tgaagtaccg cgatacagag gaggagatta
     6901 gggaggcatt cggtgtgttc gacaaggacg gcaatggata cattagtgca gcagagttgc
     6961 gccacgttat gactaacctg ggtgagaagt tgactgacga agaggtcgat gagatgatta
     7021 gggaagccga tattgacggc gatggccagg tgaactatga ggaattcgta caaatgatga
     7081 ccgccaaaga gggtcgcgga tcgctgctga cctgcggcga cgtggaggaa aaccccggcc
     7141 ccgttagcaa gggtgaagag gtgattaaag aattcatgcg ttttaaagta cgcatggagg
     7201 gtagcatgaa cggtcatgag tttgagatcg agggcgaagg cgaaggtcgt ccgtacgagg
     7261 gtacgcagac cgctaaactg aaagtaacca aaggcggtcc tctgccgttc gcgtgggaca
     7321 tcctgtcccc gcagtttatg tatggttcca aggcctacgt caaacaccca gccgatatcc
     7381 cggactacaa gaagctgtct ttcccggagg gtttcaagtg ggagcgtgta atgaactttg
     7441 aggacggtgg cctggttacc gtcacccagg atagctctct gcaagatggc accctgatct
     7501 acaaggtgaa gatgcgtggt acgaactttc cgccggatgg cccggtgatg cagaaaaaga
     7561 cgatgggatg ggaagcatct accgagcgcc tgtatccgcg tgacggtgtt ctgaaaggtg
     7621 agatccacca ggcgctgaaa ctgaaggacg gcggtcacta cctggttgaa tttaagacga
     7681 tctatatggc taaaaagccg gttcaactgc caggttacta ctacgtggac accaaactgg
     7741 atatcacgtc tcacaacgaa gattatacca tcgtcgaaca gtatgaacgt agcgagggcc
     7801 gtcaccacct gttcctgggc cacggtactg gttctacggg ttctggttcc tctggcactg
     7861 cgtcttccga agacaacaac atggcggtga tcaaagagtt catgaggttc aaagtacgta
     7921 tggaaggttc tatgaacggc catgagttcg agattgaagg tgagggcgag ggtcgcccat
     7981 acgagggcac tcagaccgca aagctgaaag ttaccaaagg tggcccactg ccttttgcgt
     8041 gggatatcct gtctccgcag ttcatgtacg gttccaaagc ctacgtaaaa cacccggcag
     8101 acatcccgga ttacaaaaag ctgtccttcc cagagggctt caaatgggaa cgtgttatga
     8161 atttcgaaga tggcggcctg gtcactgtta cgcaggacag cagcctgcaa gacggtactc
     8221 tgatttacaa agtgaaaatg cgtggcacta acttcccgcc tgacggtcca gtaatgcaga
     8281 agaaaactat gggctgggaa gctagcaccg aacgtctgta cccgcgcgac ggtgtgctga
     8341 aaggcgagat tcaccaggcc ctgaagctga aggatggtgg tcactatctg gttgagttca
     8401 aaactatcta catggcaaaa aagcctgtac aactgccggg ctattactac gttgacacta
     8461 aactggacat cacctcccac aacgaggatt atactattgt tgaacagtac gagcgttctg
     8521 aaggtcgcca tcatctgttt ctgtatggta tggacgaact gtataaataa ggtgtacaaa
     8581 tctagaatga atcgttttta aaataacaaa tcaattgttt tataatattc gtacgattct
     8641 ttgattatgt aataaaatgt gatcattagg aagattacga aaaatataaa aaatatgagt
     8701 tctgtgtgta taacaaatgc tgtaaacgcc acaattgtgt ttgttgcaaa taaacccatg
     8761 attatttgat taaaattgtt gttttctttg ttcatagaca atagtgtgtt ttgcctaaac
     8821 gtgtactgca taaactccat gcgagtgtat agcgagctag tggctaacgc ttgccccacc
     8881 aaagtagatt cgtcaaaatc ctcaatttca tcaccctcct ccaagtttaa catttggccg
     8941 tcggaattaa cttctaaaga tgccacataa tctaataaat gaaatagaga ttcaaacgtg
     9001 gcgtcatcgt ccgtttcgac catttccgaa aagaactcgg gcataaactc tatgatttct
     9061 ctggacgtgg tgttgtcgaa actctcaaag tacgcagtca ggaacgtgcg cgacatgtcg
     9121 tcgggaaact cgcgcggaaa catgttgttg taaccgaacg ggtcccatag cgccaaaacc
     9181 aaatctgcca gcgtcaatag aatgagcacg atgccgacaa tggagctggc ttggatagcg
     9241 attcgagtta acggccggcc gtttaaacga attcttgaag acgaaagggc ctcgtgatac
     9301 gcctattttt ataggttaat gtcatgataa taatggtttc ttagactcag gtggcacttt
     9361 tcggggaaag gttgcttaag aataagtaat tcttaagcaa ccattacgcc aagcttgcat
     9421 gcaggcctct gcagtcgacg ggcccgggat ccgatcgaca agccgaacat atgggccagt
     9481 gaattcgagc tcggtacctc gcgaatgcat ctagatgcac cattatgttc cggaggcgcg
     9541 ccatcgatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     9601 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     9661 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     9721 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     9781 atgatcttta gagaaagaga tggtgacgac cttcagtgcg ttcatattgc tcaactatcg
     9841 tataatcttc gttatgtgaa gtaatatcca gtttcgaatc gacataataa tatccaggga
     9901 gttggaccgg tttcttagcc atataaatgc ttttaaattc tacaaggtaa tgtccgccat
     9961 ctttgagttt aagagctttg tgaatttcgc ctttcaaaac tccatctcgt ggatacagac
    10021 gttccgtcga tgcttcccaa cccattgttt tcttttgcat aaccggtcca tccgacggaa
    10081 aattgactcc aataaatttc actttgtaaa taaaacaacc atcttggagc gaagaatctt
    10141 gtgtgactgt gacaactcca ccatcttcga aattcatcac gcgctcccac ttgaagccct
    10201 cggggaagga cagcttcttg tagtcgggga tgtcggcggg gtgcttcacg tacaccttgg
    10261 agccgtactg gaactggggg gacaggatgt cccaggcgaa gggcaggggg ccgcccttgg
    10321 tcaccttcag cttcacggtg ttgtggccct cgtaggggcg gccctcgccc tcgccctcga
    10381 tctcgaactc gtggccgttc acggtgccct ccatgcgcac cttgaagcgc atgaactcct
    10441 tgatgacgtt cttggaggag cgcaccatgg ggtggcgacc ggtggatcgt accgtcgact
    10501 ctagcggtac cccgattgtt tagcttgttc agctgcgctt gtttatttgc ttagctttcg
    10561 cttagcgacg tgttcacttt gcttgtttga attgaattgt cgctccgtag acgaagcgcc
    10621 tctatttata ctccggcggt cgagggttaa tggtcacttg gttgttcacg atcttgtcgc
    10681 cgccagtgtc aacttgcaac tgaaacaata tccaacatga acgtcaattt atactgccct
    10741 aatggcgaac acgataacaa tatttctttt attatgccct ctaaaaccaa cgcggttatc
    10801 gtttatttat tcaaattaga tatagaacat ccgccgacat acaatgttaa tgcaaaaacg
    10861 cgtttggtga gcggatacga aaacagtcgg ccgataaaca ttaatctgag gtcgataaca
    10921 ccgtccttga acggaacacg aggagcgtac gtgatcagct gcattcgcgc gccgcgcctt
    10981 tatcgagatt tatttgcata caacaagtac actgcgccgt tgggatttgt ggtaacgcgc
    11041 acacatgcag agctgcaagt gtggcacatt ttgtctgtgc gcaaaacctt tgaagccaaa
    11101 agtacgaggt ccgttacggg catgctagcg cacacggaca atggacccga caaattctac
    11161 gccaaggatt taatgataat gtcgggcaac gtatccgttc attttatcaa taacctacaa
    11221 aaatgtcgcg cgcatcacaa agacatcgac gcgcgtagaa ttctacccgt aaagcgagtt
    11281 tagttatgag ccatgtgcaa aacatgacat catctcgatt tgaaaaacaa atgacatcat
    11341 ccactgatcg tgcgttacaa gtagaattct actcgtaaag ccagttcggt tatgagccgt
    11401 gtgcaaaaca tgacatcagc ttatgactca tacttgattg tgttttacgc gggcgcgtaa
    11461 gagctcggcc tccggaacat aatggtgcat ctagatgcat tcgcgaggta ccgagctcga
    11521 attcactggg gttgcttaag aataagtaat tcttaagcaa cccaacccca tcaactttgt
    11581 atacaaaagt tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
    11641 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatgggccg
    11701 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
    11761 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
    11821 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
    11881 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
    11941 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
    12001 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
    12061 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
    12121 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
    12181 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtgtcag
    12241 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
    12301 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
    12361 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
    12421 aattacaaca gtactgcgat gagtggcagg gcggggcgta aacgcgtggc acatatacct
    12481 gccgttcact attatttagt gaaatgagat attatgatat tttctgaatt gtgattaaaa
    12541 aggcaacttt atgcccatgc aacagaaact ataaaaaata cagagaatga aaagaaacag
    12601 atagattttt tagttcttta ggcccgtagt ctgcaaatcc ttttatgatt ttctatcaaa
    12661 caaaagagga aaatagacca gttgcaatcc aaacgagagt ctaatagaat gaggtcgaaa
    12721 agtaaatcgc gcgggtttgt tactgataaa gcaggcaaga cctaaaatgt gtaaagggca
    12781 aagtgtatac tttggcgtca ccccttacat attttaggtc tttttttatt gtgcgtaact
    12841 aacttgccat cttcaaacag gagggctgga agaagcagac cgctaacaca gtacataaaa
    12901 aaggagacat gaacgatgaa catcaaaaag tttgcaaaac aagcaacagt attaaccttt
    12961 actaccgcac tgctggcagg aggcgcaact caagcgtttg cgaaagaaac gaaccaaaag
    13021 ccatataagg aaacatacgg catttcccat attacacgcc atgatatgct gcaaatccct
    13081 gaacagcaaa aaaatgaaaa atatcaagtt cctgaattcg attcgtccac aattaaaaat
    13141 atctcttctg caaaaggcct ggacgtttgg gacagctggc cattacaaaa cgctgacggc
    13201 actgtcgcaa actatcacgg ctaccacatc gtctttgcat tagccggaga tcctaaaaat
    13261 gcggatgaca catcgattta catgttctat caaaaagtcg gcgaaacttc tattgacagc
    13321 tggaaaaacg ctggccgcgt ctttaaagac agcgacaaat tcgatgcaaa tgattctatc
    13381 ctaaaagacc aaacacaaga atggtcaggt tcagccacat ttacatctga cggaaaaatc
    13441 cgtttattct acactgattt ctccggtaaa cattacggca aacaaacact gacaactgca
    13501 caagttaacg tatcagcatc agacagctct ttgaacatca acggtgtaga ggattataaa
    13561 tcaatctttg acggtgacgg aaaaacgtat caaaatgtac agcagttcat cgatgaaggc
    13621 aactacagct caggcgacaa ccatacgctg agagatcctc actacgtaga agataaaggc
    13681 cacaaatact tagtatttga agcaaacact ggaactgaag atggctacca aggcgaagaa
    13741 tctttattta acaaagcata ctatggcaaa agcacatcat tcttccgtca agaaagtcaa
    13801 aaacttctgc aaagcgataa aaaacgcacg gctgagttag caaacggcgc tctcggtatg
    13861 attgagctaa acgatgatta cacactgaaa aaagtgatga aaccgctgat tgcatctaac
    13921 acagtaacag atgaaattga acgcgcgaac gtctttaaaa tgaacggcaa atggtacctg
    13981 ttcactgact cccgcggatc aaaaatgacg attgacggca ttacgtctaa cgatatttac
    14041 atgcttggtt atgtttctaa ttctttaact ggcccataca agccgctgaa caaaactggc
    14101 cttgtgttaa aaatggatct tgatcctaac gatgtaacct ttacttactc acacttcgct
    14161 gtacctcaag cgaaaggaaa caatgtcgtg attacaagct atatgacaaa cagaggattc
    14221 tacgcagaca aacaatcaac gtttgcgcca agcttcctgc tgaacatcaa aggcaagaaa
    14281 acatctgttg tcaaagacag catccttgaa caaggacaat taacagttaa caaataaatg
    14341 tcaggctccc ttatacacag ccagtctgca cgtcgcatag tgactggata tgttgtgttt
    14401 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
    14461 cattttacgt ttctcgttca actttttaat acaaagttgc ctccggaaca taatggtgca
    14521 tctagatgcc cattcctgta gacttcttaa ttaagacgtc agaattctcg aggcggccgc
    14581 atgtgagtct ccctatagtg agtcgtatta atttcgcggg cgcgtcagcg ggagagccgt
    14641 tgtaaggcgg ca
//
