LOCUS       pJAT75{dU6-3-attR3-Amp-ccdB-attR4-ie1-EGFP,_hs-Flp1-attR5-Chlor-SacB-attR6}       12462 bp    DNA     circular UNA 12-JUN-2023
DEFINITION  Generated by Golden Gate/Type IIS Cloning (P767
            p{dU6-3-GW1-Amp-ccdB-Mhc-GFP-FRT-P3-DsRed-GW2-Chlor-SacB}, P545
            pBac{ie1-EGFP,PhsFlp1} PCR Product 3, P545 PhsFlp1).
ACCESSION   urn.local.sternd.20r-gdru11z
VERSION     urn.local.sternd.20r-gdru11z
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             411..1202
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /standard_name="SmR"
     rep_origin      1448..2036
     misc_feature    1820..1837
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ori-252 R"
     primer_bind     1937..1956
                     /note="pBR322 origin, forward primer"
                     /standard_name="pBR322ori-F"
     primer_bind     2026..2045
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7Cas9F"
     primer_bind     complement(2052..2071)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp-ori-7571F"
     misc_feature    2180..2209
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     2190..2207
                     /note="L4440 vector, forward primer"
                     /standard_name="L4440"
     regulatory      2239..2258
                     /note="Geneious type: promoter"
                     /note="SP6 RNA polymerase promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SP6"
     misc_feature    2300..2303
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2304..2333
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     regulatory      2334..2754
                     /note="Geneious type: promoter"
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
                     /standard_name="dU6-3 promoter"
     misc_feature    2755..2787
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    2758..2787
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2761..2884
                     /note="Geneious type: attR3"
                     /standard_name="attR3"
     misc_feature    2976..2979
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      2980..3084
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     gene            3085..3945
                     /standard_name="AmpR"
     primer_bind     complement(3125..3146)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AMP_5'_F"
     misc_feature    3331..3350
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_R"
     misc_feature    3804
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="T"
     primer_bind     complement(3817..3836)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_F"
     misc_feature    3909..3932
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp_3'_R"
     primer_bind     complement(3922..3945)
                     /standard_name="966 R"
     misc_feature    3942..3945
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    3946..4585
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGT
                     TGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAAT
                     GAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
                     AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTG
                     AAATGCAATTTAAGGTTTATACTTACAAGCGCGAGTCCCGCTATCGCCTTTTCGTGGA
                     CGTCCAGTCAGACATTATCGACACGCCTGGTCGCCGTATGGTGATTCCATTAGCCAGC
                     GCACGCCTGTTAAGTGATAAGGTGTCGCGCGAGCTTTACCCAGTCGTCCATATTGGTG
                     ACGAGAGTTGGCGCATGATGACTACAGACATGGCTAGTGTACCTGTGTCGGTGATCGG
                     GGAAGAGGTCGCGGATTTAAGCCACCGTGAGAATGATATTAAGAATGCAATCAATCTC
                     ATGTTTTGGGGTATTTA"
     primer_bind     3946..3967
                     /standard_name="1 F"
     gene            4255..4587
                     /standard_name="ccdB"
     misc_feature    complement(4625..4748)
                     /note="Geneious type: attR4"
                     /standard_name="attR4"
     misc_feature    4752..7091
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P545 pBac{ie1-EGFP,PhsFlp1} PCR Product 3
                     (concatenated sequence 2)"
     misc_feature    4752..4755
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    <4756..4767
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    <4756..4763
                     /note="piggyBac right arm"
                     /standard_name="piggyBac right arm"
     primer_bind     4756..4775
                     /standard_name="1 F"
     misc_feature    4768..5867
                     /locus_tag="ie1 promoter"
                     /note="Geneious type: Enhancer"
                     /standard_name="ie1"
     misc_feature    5853..5882
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    6053..6850
                     /note="Geneious type: Reporter"
                     /standard_name="EGFP-F reporter"
     misc_feature    6053..6774
                     /note="Geneious type: Reporter"
                     /standard_name="d2EYFP reporter"
     misc_feature    6053..6769
                     /note="Geneious type: Reporter"
                     /standard_name="EGFP reporter"
     misc_feature    6056..6790
                     /note="Geneious type: Reporter"
                     /standard_name="EYFP reporter"
     regulatory      6788..7032
                     /note="Geneious type: Terminator"
                     /standard_name="SV40_PA term"
     misc_feature    7018..7047
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     complement(7072..7091)
                     /standard_name="2,336 R"
     misc_feature    7088..7091
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    7088..9385
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P545 PhsFlp1 (concatenated sequence 3)"
     primer_bind     7092..7111
                     /standard_name="1 F"
     misc_feature    complement(7305..7550)
                     /note="Geneious type: polyA_signal"
                     /standard_name="hsp70 terminator"
     CDS             complement(7565..8836)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWM
                     ITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEF
                     TIIPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEK
                     ILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCL
                     VTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLK
                     DNLVRSYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKR
                     ASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKG
                     SAEGSIRYPAWNGIISQEVLDYLSSYINRRI"
                     /standard_name="FLP"
     regulatory      complement(8892..9130)
                     /note="Geneious type: promoter"
                     /note="Drosophila melanogaster hsp70Bb promoter"
                     /standard_name="hsp70 promoter"
     misc_feature    complement(9125..9344)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="HS enhancer"
     primer_bind     complement(9362..9381)
                     /standard_name="2,290 R"
     misc_feature    9382..9385
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    9382..4755
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P767
                     p{dU6-3-GW1-Amp-ccdB-Mhc-GFP-FRT-P3-DsRed-GW2-Chlor-SacB}
                     (concatenated sequence 1)"
     misc_feature    9382..9505
                     /note="Geneious type: attR5"
                     /standard_name="attR5"
     gene            9612..10271
                     /gene="CAT/CamR"
                     /standard_name="CAT/CamR"
     misc_feature    9617..10269
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AAAAAAGATTACGGGTTATACTACCGTCGACATCAGCCAGTG
                     GCACCGTAAAGAACATTTCGAAGCGTTCCAGAGCGTCGCGCAGTGCACCTACAATCAG
                     ACCGTTCAGCTGGATATCACTGCTTTCCTGAAGACTGTTAAGAAAAACAAACACAAAT
                     TTTATCCGGCTTTTATCCACATTCTCGCTCGCCTGATGAACGCACACCCGGAATTCCG
                     TATGGCCATGAAAGATGGCGAGCTGGTAATTTGGGATTCCGTTCATCCGTGTTATACC
                     GTCTTTCACGAACAGACCGAGACGTTCTCTTCCCTGTGGTCTG"
     misc_feature    10274..12146
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CGAGACGTTCTCTTCCCTGTGGTCTGAATATCACGATGACTT
                     CCGTCAATTCCTGCACATTTATTCTCAGGACGTTGCGTGCTATGGTGAGAATCTCGCT
                     TATTTTCCGAAGGGTTTCATTGAAAACATGTTCTTTGTTAGCGCCAACCCATGGGTGA
                     GCTTCACTTCTTTCGACCTGAACGTGGCGAACATGGACAACTTTTTCGCGCCGGTTTT
                     CACGATGGGCAAGTATTACACCCAGGGTGACAAGGTACTGATGCCGCTGGCAATCCAG
                     GTGCATCACGCGGTATGTGACGGCTTCCATGTCGGTCGCATGCTGAACGAGCTGCAGC
                     AATACTGTGATGAATGGCAGGGCGGTGCGTAAACGCGTGGATCCGGCTTACTAAAAGC
                     CAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGAT
                     ATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGAC
                     AGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTC
                     TGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGC
                     GGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTT
                     GCTGACGAGAACAGGGGCTGGTGAAATGCAATTTAAAGTTTATACCTACAAGCGCGAA
                     TCTCGCTACCGTCTGTTTGTAGACGTACAGTCTGATATCATTGATACGCCGGGCCGCC
                     GTATGGTTATTCCGCTGGCATCTGCACGTCTCCTGTCTGATAAAGTTAGCCGTGAACT
                     GTATCCGGTTGTACACATTGGCGACGAGTCTTGGCGCATGATGACGACTGACATGGCT
                     TCTGTCCCGGTTAGCGTCATTGGCGAGGAAGTCGCCGATCTGTCTCACCGTGAAAATG
                     ACATCAAAAATGCAATTAACCTGATGTTCTGGGGCATTTGATCCGGCTTACTAAAAGC
                     CAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGAT
                     ATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGAC
                     AGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTC
                     TGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGC
                     GGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTT
                     GCTGACGAGAACAGGGGCTGGTGAAATGCAATTTAAAGTTTATACCTACAAGCGCGAA
                     TCTCGCTACCGTCTGTTTGTAGACGTACAGTCTGATATCATTGATACGCCGGGCCGCC
                     GTATGGTTATTCCGCTGGCATCTGCACGTCTCCTGTCTGATAAAGTTAGCCGTGAACT
                     GTATCCGGTTGTACACATTGGCGACGAGTCTTGGCGCATGATGACGACTGACATGGCT
                     TCTGTCCCGGTTAGCGTCATTGGCGAGGAAGTCGCCGATCTGTCTCACCGTGAAAATG
                     ACATCAAAAATGCAATTAACCTGATGTTCTGGGGCATTTG"
     misc_feature    10276..10279
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      10280..10725
                     /note="Geneious type: promoter"
                     /note="Bacillus subtilis sacB (glycoside hydrolase family
                     68) promoter region"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB"
     gene            10726..12149
                     /note="counterselectable marker from Bacillus subtilis
                     that converts sucrose to levans"
                     /product="levansucrase"
                     /gene="sacB"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB CS"
     misc_feature    11117..11120
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    complement(12186..12309)
                     /note="Geneious type: attR6"
                     /standard_name="attR6"
     misc_feature    12310..12339
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    12340..12343
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      complement(12403..12420)
                     /regulatory_class="promoter"
                     /note="T7 promoter"
                     /standard_name="T7 promoter"
     misc_feature    12433..12462
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
ORIGIN      
        1 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
       61 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
      121 tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
      181 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
      241 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
      301 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      361 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
      421 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
      481 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
      541 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
      601 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
      661 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
      721 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
      781 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
      841 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
      901 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
      961 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     1021 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1081 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     1141 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     1201 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     1261 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     1321 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca
     1381 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1441 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1501 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1561 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1621 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1681 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1741 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1801 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1861 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1921 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1981 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2041 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2101 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2161 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa atcgtcacct
     2221 gcaggaaggt ttaaacgcat ttaggtgaca ctatagaagt gtgtatcgct cgagggatcc
     2281 gaattcgaag tcttggtacg gaggggatcc gatcgacaag ccgaacatat gggttttttg
     2341 ctcacctgtg attgctccta ctcaaataca aaaacatcaa attttctgtc aataaagcat
     2401 atttatttat atttatttta caggaaagaa ttccttttaa agtgtatttt aacctataat
     2461 gaaaaacgat taaaaaaaat acataaaata attcgaaaat ttttgaatag cccaggttga
     2521 taaaaattca tttcatacgt tttataactt atgcccctaa gtattttttg accatagtgt
     2581 ttcaattcta cattaatttt acagagtaga atgaaacgcc acctactcag ccaagaggcg
     2641 aaaaggttag ctcgccaagc agagagggcg ccagtgctca ctacttttta taattctcaa
     2701 cttctttttc cagactcagt tcgtatatat agacctattt tcaatttaac gtcggggcct
     2761 caactttgta taataaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     2821 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     2881 tatgggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
     2941 ggattttgag ttaggatccg tcgagatttt caggagctac gcggaacccc tatttgttta
     3001 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3061 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3121 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3181 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3241 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3301 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3361 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3421 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3481 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3541 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3601 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3661 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3721 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3781 tctggagccg gtgagcgtgg gtcccgcggt atcattgcag cactggggcc agatggtaag
     3841 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3901 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaatggat ccggcttact
     3961 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     4021 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     4081 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     4141 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     4201 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     4261 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     4321 tatcgtctgt ttgtggatgt acagagtgat attatcgata caccgggccg tcgcatggtt
     4381 attccacttg cgtctgcgcg cttgctctca gataaagtct cccgtgaact ttacccggtg
     4441 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtt
     4501 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     4561 attaacctga tgttctgggg aatataatgt caggctccct tatacacagc cagtctgcac
     4621 gtcgcatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     4681 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa cttttctata
     4741 caaagttgtc cagctcttcc tactgcaccg agctcttacg cgcccgcgta aaacacaatc
     4801 aagtatgagt cataagctga tgtcatgttt tgcacacggc tcataaccga actggcttta
     4861 cgagtagaat tctacttgta acgcacgatc agtggatgat gtcatttgtt tttcaaatcg
     4921 agatgatgtc atgttttgca cacggctcat aaactcgctt tacgagtaga attctacgtg
     4981 taacgcacga tcgattgatg agtcatttgt tttgcaatat gatatcatac aatatgactc
     5041 atttgttttt caaaaccgaa cttgatttac gggtagaatt ctacttgtaa agcacaatca
     5101 aaaagatgat gtcatttgtt tttcaaaact gaactcgctt tacgagtaga attctacgtg
     5161 taaaacacaa tcaagaaatg atgtcatttg ttataaaaat aaaagctgat gtcatgtttt
     5221 gcacatggct cataactaaa ctcgctttac gggtagaatt ctacgcgcgt cgatgtcttt
     5281 gtgatgcgcg cgacattttt gtaggttatt gataaaatga acggatacgt tgcccgacat
     5341 tatcattaaa tccttggcgt agaatttgtc gggtccattg tccgtgtgcg ctagcatgcc
     5401 cgtaacggac ctcgtacttt tggcttcaaa ggttttgcgc acagacaaaa tgtgccacac
     5461 ttgcagctct gcatgtgtgc gcgttaccac aaatcccaac ggcgcagtgt acttgttgta
     5521 tgcaaataaa tctcgataaa ggcgcggcgc gcgaatgcag ctgatcacgt acgctcctcg
     5581 tgttccgttc aaggacggtg ttatcgacct cagattaatg tttatcggcc gactgttttc
     5641 gtatccgctc accaaacgcg tttttgcatt aacattgtat gtcggcggat gttctatatc
     5701 taatttgaat aaataaacga taaccgcgtt ggttttagag ggcataataa aagaaatatt
     5761 gttatcgtgt tcgccattag ggcagtataa attgacgttc atgttggata ttgtttcagt
     5821 tgcaagttga cactggcggc gacaagatcg tgaacaacca agtgaccctc gaccgccgga
     5881 gtataaatag aggcgcttcg tctacggagc gacaattcaa ttcaaacaag caaagtgaac
     5941 acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc tgaacaagct aaacaatcgg
     6001 ggtaccgcta gagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag
     6061 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     6121 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     6181 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     6241 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     6301 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     6361 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     6421 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     6481 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     6541 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     6601 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     6661 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     6721 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg
     6781 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     6841 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     6901 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6961 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaagc
     7021 ttatcgatac gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt
     7081 gctcaggtcg cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca
     7141 gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc agcggccgcg
     7201 tttaaacacc ggttagggat gccaactcgt gcttttaagc gcgtaaagtt ttcgattcgg
     7261 cgccaactct ccatcgcatt taaataaata atttctcgag gacagatcta aacgagtttt
     7321 taagcaaact cactccctga caataaaaac gcaattatct ttaaataaat atattctatt
     7381 tattaaccaa gtagctaata tagctaaaat caatttgttg ctaacttaaa actaacataa
     7441 ttatgtatta gttatttaca ttcttttggt tggttacaac caaataaata atatcacttt
     7501 taaaaactta agccaggaac tgaataattt tctatttggc tttagtcgac cctagggttt
     7561 aaacttatat gcgtctattt atgtaggatg aaaggtagtc tagtacctcc tgtgatatta
     7621 tcccattcca tgcggggtat cgtatgcttc cttcagcact accctttagc tgttctatat
     7681 gctgccactc ctcaattgga ttagtctcat ccttcaatgc tatcatttcc tttgatattg
     7741 gatcatatgc atagtaccga gaaactagtg cgaagtagtg atcaggtatt gctgttatct
     7801 gatgagtata cgttgtcctg gccacggcag aagcacgctt atcgctccaa tttcccacaa
     7861 cattagtcaa ctccgttagg cccttcattg aaagaaatga ggtcatcaaa tgtcttccaa
     7921 tgtgagattt tgggccattt tttatagcaa agattgaata aggcgcattt ttcttcaaag
     7981 ctttattgta cgatctgact aagttatctt ttaataattg gtattcctgt ttattgcttg
     8041 aagaattgcc ggtcctattt actcgtttta ggactggttc agaattcctc aaaaattcat
     8101 ccaaatatac aagtggatcg atcctacccc ttgcgctaaa gaagtatatg tgcctactaa
     8161 cgcttgtctt tgtctctgtc actaaacact ggattattac tcccagatac ttattttgga
     8221 ctaatttaaa tgatttcgga tcaacgttct taatatcgct gaatcttcca caattgatga
     8281 aagtagctag gaagaggaat tggtataaag tttttgtttt tgtaaatctc gaagtatact
     8341 caaacgaatt tagtattttc tcagtgatct cccagatgct ttcaccctca cttagaagtg
     8401 ctttaagcat ttttttactg tggctatttc ccttatctgc ttcttccgat gattcgaact
     8461 gtaattgcaa actacttaca atatcagtga tatcagattg atgtttttgt ccatagtaag
     8521 gaataattgt aaattcccaa gcaggaatca atttctttaa tgaggcttcc agaattgttg
     8581 ctttttgcgt cttgtattta aactggagtg atttattgac aatatcgaaa ctcagcgaat
     8641 tgcttatgat agtattatag ctcatgaatg tggctctctt gattgctgtt ccgttatgtg
     8701 taatcatcca acataaatag gttagttcag cagcacataa tgctattttc tcacctgaag
     8761 gtctttcaaa cctttccaca aactgacgaa caagcacctt aggtggtgtt ttacataata
     8821 tatcaaattg tggcattttg attgctagcg gtacctgagt tcttctttct cggtaacttg
     8881 ttgaaagtat tcagagttct cttcttgtat tcaataatta cttcttggca gatttcagta
     8941 gttgcagttg atttacttgg ttgctggtta cttttaattg attcacttta acttgcactt
     9001 tactgcagat tgtttagctt gttcagctgc gcttgtttat ttgcttagct ttcgcttagc
     9061 gacgtgttca ctttgcttgt ttgaattgaa ttgtcgctcc gtagacgaag cgcctctatt
     9121 tatactccgg cgctcttttc gcgaacattc gaggcgcgct ctctcgaacc aacgagagca
     9181 gtatgtcgtt tactgtgtga cagagtgaga gagcattagt gcagagaggg agagacccaa
     9241 aaagaaaaga gacaataacg aataacggcc agagaaattt ctcgagtttt ctttctgcca
     9301 aacaaatgac ctaccacaat aaccagtttg ttttgggatt cagatctggc cggccgaatt
     9361 cttgaagacg aaagggcctc gcaactttgt atacaaaagt tgaacgagaa acgtaaaatg
     9421 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
     9481 aaacacaaca tatccagtca ctatgggccg cattaggcac cccaggcttt acactttatg
     9541 cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta
     9601 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     9661 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     9721 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     9781 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
     9841 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     9901 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     9961 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
    10021 ggtttattga gaatatgttt ttcgtgtcag ccaatccctg ggtgagtttc accagttttg
    10081 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
    10141 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
    10201 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
    10261 gcggggcgta aacgcgtggc acatatacct gccgttcact attatttagt gaaatgagat
    10321 attatgatat tttctgaatt gtgattaaaa aggcaacttt atgcccatgc aacagaaact
    10381 ataaaaaata cagagaatga aaagaaacag atagattttt tagttcttta ggcccgtagt
    10441 ctgcaaatcc ttttatgatt ttctatcaaa caaaagagga aaatagacca gttgcaatcc
    10501 aaacgagagt ctaatagaat gaggtcgaaa agtaaatcgc gcgggtttgt tactgataaa
    10561 gcaggcaaga cctaaaatgt gtaaagggca aagtgtatac tttggcgtca ccccttacat
    10621 attttaggtc tttttttatt gtgcgtaact aacttgccat cttcaaacag gagggctgga
    10681 agaagcagac cgctaacaca gtacataaaa aaggagacat gaacgatgaa catcaaaaag
    10741 tttgcaaaac aagcaacagt attaaccttt actaccgcac tgctggcagg aggcgcaact
    10801 caagcgtttg cgaaagaaac gaaccaaaag ccatataagg aaacatacgg catttcccat
    10861 attacacgcc atgatatgct gcaaatccct gaacagcaaa aaaatgaaaa atatcaagtt
    10921 cctgaattcg attcgtccac aattaaaaat atctcttctg caaaaggcct ggacgtttgg
    10981 gacagctggc cattacaaaa cgctgacggc actgtcgcaa actatcacgg ctaccacatc
    11041 gtctttgcat tagccggaga tcctaaaaat gcggatgaca catcgattta catgttctat
    11101 caaaaagtcg gcgaaacttc tattgacagc tggaaaaacg ctggccgcgt ctttaaagac
    11161 agcgacaaat tcgatgcaaa tgattctatc ctaaaagacc aaacacaaga atggtcaggt
    11221 tcagccacat ttacatctga cggaaaaatc cgtttattct acactgattt ctccggtaaa
    11281 cattacggca aacaaacact gacaactgca caagttaacg tatcagcatc agacagctct
    11341 ttgaacatca acggtgtaga ggattataaa tcaatctttg acggtgacgg aaaaacgtat
    11401 caaaatgtac agcagttcat cgatgaaggc aactacagct caggcgacaa ccatacgctg
    11461 agagatcctc actacgtaga agataaaggc cacaaatact tagtatttga agcaaacact
    11521 ggaactgaag atggctacca aggcgaagaa tctttattta acaaagcata ctatggcaaa
    11581 agcacatcat tcttccgtca agaaagtcaa aaacttctgc aaagcgataa aaaacgcacg
    11641 gctgagttag caaacggcgc tctcggtatg attgagctaa acgatgatta cacactgaaa
    11701 aaagtgatga aaccgctgat tgcatctaac acagtaacag atgaaattga acgcgcgaac
    11761 gtctttaaaa tgaacggcaa atggtacctg ttcactgact cccgcggatc aaaaatgacg
    11821 attgacggca ttacgtctaa cgatatttac atgcttggtt atgtttctaa ttctttaact
    11881 ggcccataca agccgctgaa caaaactggc cttgtgttaa aaatggatct tgatcctaac
    11941 gatgtaacct ttacttactc acacttcgct gtacctcaag cgaaaggaaa caatgtcgtg
    12001 attacaagct atatgacaaa cagaggattc tacgcagaca aacaatcaac gtttgcgcca
    12061 agcttcctgc tgaacatcaa aggcaagaaa acatctgttg tcaaagacag catccttgaa
    12121 caaggacaat taacagttaa caaataaatg tcaggctccc ttatacacag ccagtctgca
    12181 cgtcgcatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
    12241 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca actttttaat
    12301 acaaagttgc ctccggaaca taatggtgca tctagatgcc cattcctgta gacttcttaa
    12361 ttaagacgtc agaattctcg aggcggccgc atgtgagtct ccctatagtg agtcgtatta
    12421 atttcgcggg cgcgtcagcg ggagagccgt tgtaaggcgg ca
//
