LOCUS       pJAT25{dU6-3-attR3-Amp-ccdB-attR4-ie1-MBP-GFPc-attR5-Chlor-SacB-attR6}       10390 bp    DNA     circular UNA 12-JUN-2023
DEFINITION  Golden Gate/Type IIS Cloning ligation product.
ACCESSION   urn.local.sternd.21j-gdru6ew
VERSION     urn.local.sternd.21j-gdru6ew
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..4770
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P859
                     p{dU6-3-GW1-Amp-ccdB-MCS-GW2-Chlor-SacB} extraction
                     (concatenated sequence 1)"
     CDS             411..1202
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /standard_name="SmR"
     rep_origin      1448..2036
     misc_feature    1820..1837
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ori-252 R"
     primer_bind     1937..1956
                     /note="pBR322 origin, forward primer"
                     /standard_name="pBR322ori-F"
     primer_bind     2026..2045
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7Cas9F"
     primer_bind     complement(2052..2071)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp-ori-7571F"
     misc_feature    2180..2209
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     2190..2207
                     /note="L4440 vector, forward primer"
                     /standard_name="L4440"
     regulatory      2239..2258
                     /note="Geneious type: promoter"
                     /note="SP6 RNA polymerase promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SP6"
     misc_feature    2300..2303
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2304..2333
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     regulatory      2334..2754
                     /note="Geneious type: promoter"
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
                     /standard_name="dU6-3 promoter"
     misc_feature    complement(2399..2409)
                     /note="Geneious type: Transcription Factor"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Hb"
     misc_feature    2755..2787
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    2756
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     misc_feature    2757
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     misc_feature    2758..2787
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2761..2884
                     /note="Geneious type: attR3"
                     /standard_name="attR3"
     regulatory      2907..2937
                     /note="Geneious type: promoter"
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacUV5"
     misc_feature    complement(2918..2929)
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     misc_feature    2976..2979
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2979
                     /note="Geneious type: Junction"
                     /standard_name="junction 2"
     misc_feature    2979
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     regulatory      2980..3084
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     gene            3085..3945
                     /standard_name="AmpR"
     primer_bind     complement(3125..3146)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AMP_5'_F"
     misc_feature    3331..3350
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_R"
     misc_feature    3804
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="T"
     primer_bind     complement(3817..3836)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_F"
     misc_feature    3909..3932
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp_3'_R"
     primer_bind     complement(3922..3945)
                     /standard_name="966 R"
     misc_feature    3942..3945
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     3946..3967
                     /standard_name="1 F"
     misc_feature    3946..4585
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGT
                     TGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAAT
                     GAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
                     AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTG
                     AAATGCAATTTAAGGTTTATACTTACAAGCGCGAGTCCCGCTATCGCCTTTTCGTGGA
                     CGTCCAGTCAGACATTATCGACACGCCTGGTCGCCGTATGGTGATTCCATTAGCCAGC
                     GCACGCCTGTTAAGTGATAAGGTGTCGCGCGAGCTTTACCCAGTCGTCCATATTGGTG
                     ACGAGAGTTGGCGCATGATGACTACAGACATGGCTAGTGTACCTGTGTCGGTGATCGG
                     GGAAGAGGTCGCGGATTTAAGCCACCGTGAGAATGATATTAAGAATGCAATCAATCTC
                     ATGTTTTGGGGTATTTA"
     gene            4255..4587
                     /standard_name="ccdB"
     primer_bind     4446..4465
                     /standard_name="4446F"
     misc_feature    4585
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     misc_feature    complement(4625..4748)
                     /note="Geneious type: attR4"
                     /standard_name="attR4"
     primer_bind     4741..4760
                     /standard_name="4741F"
     misc_feature    4748
                     /note="Geneious type: Junction"
                     /standard_name="junction 2"
     misc_feature    4750
                     /note="Geneious type: Junction"
                     /standard_name="junction 2"
     misc_feature    join(4752..>4770,<7295..7313)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pGGAselect-Spect extraction (concatenated
                     sequence 2)"
     misc_feature    4752..4755
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    join(<4756..>4770,<7295..>7309)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pGGAselect PCR Product (concatenated
                     sequence 2)"
     misc_feature    4756..4759
                     /note="4 base BsaI overhang created during Golden Gate
                     assembly (BsaI site 320-325)"
                     /standard_name="BsaI overhang"
     misc_feature    join(4760..>4770,<7295..7305)
                     /note="DNA sequence removed and replaced during Golden
                     Gate assembly with BsaI"
                     /standard_name="Fragment removed by BsaI"
     misc_feature    4761..4766
                     /standard_name="BsaI"
     misc_feature    4767..5882
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="RC12b_pBac_ie1_m PCR Product (concatenated
                     sequence 2)"
     misc_feature    4767..4770
                     /note="4 base BbsI overhang created during Golden Gate
                     assembly (BbsI site 343-348)"
                     /standard_name="BbsI overhang"
     misc_feature    4767..4770
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     4771..4790
                     /standard_name="ie1-F"
     misc_feature    4783..5882
                     /locus_tag="ie1 promoter"
                     /note="Geneious type: Enhancer"
                     /standard_name="ie1"
     primer_bind     complement(5861..5882)
                     /standard_name="ie1-R"
     misc_feature    5879..7298
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="MBP-GFPc-SV40 extraction (concatenated
                     sequence 3)"
     misc_feature    5879..5882
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     gene            5883..7049
                     /standard_name="MBP-GFPc"
     misc_feature    <5883..>7049
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="MBP-GFPc-SV40 extraction (concatenated
                     sequence 3)"
     misc_feature    5904..7049
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CTGGTGATCTGGATCAACGGCGATAAGGGCTACAACGGCCTG
                     GCCGAGGTGGGCAAGAAGTTCGAGAAGGATACCGGCATCAAGGTGACCGTGGAGCACC
                     CCGATAAGCTGGAGGAGAAGTTCCCCCAGGTGGCCGCCACCGGCGATGGCCCCGATAT
                     CATCTTCTGGGCCCACGATCGCTTCGGCGGCTACGCCCAGAGCGGCCTGCTGGCCGAG
                     ATCACCCCCGATAAGGCCTTCCAGGATAAGCTGTACCCCTTCACCTGGGATGCCGTGC
                     GCTACAACGGCAAGCTGATCGCCTACCCCATCGCCGTGGAGGCCCTGAGCCTGATCTA
                     CAACAAGGATCTGCTGCCCAACCCCCCCAAGACCTGGGAGGAGATCCCCGCCCTGGAT
                     AAGGAGCTGAAGGCCAAGGGCAAGAGCGCCCTGATGTTCAACCTGCAGGAGCCCTACT
                     TCACCTGGCCCCTGATCGCCGCCGATGGCGGCTACGCCTTCAAGTACGAGAACGGCAA
                     GTACGATATCAAGGATGTGGGCGTGGATAACGCCGGCGCCAAGGCCGGCCTGACCTTC
                     CTGGTGGATCTGATCAAGAACAAGCACATGAACGCCGATACCGATTACAGCATCGCCG
                     AGGCCGCCTTCAACAAGGGCGAGACCGCCATGACCATCAACGGCCCCTGGGCCTGGAG
                     CAACATCGATACCAGCAAGGTGAACTACGGCGTGACCGTGCTGCCCACCTTCAAGGGC
                     CAGCCCAGCAAGCCCTTCGTGGGCGTGCTGAGCGCCGGCATCAACGCCGCCAGCCCCA
                     ACAAGGAGCTGGCCAAGGAGTTCCTGGAGAACTACCTGCTGACCGATGAGGGCCTGGA
                     GGCCGTGAACAAGGATAAGCCCCTGGGCGCCGTGGCCCTGAAGAGCTACGAGGAGGAG
                     CTGGTGAAGGATCCCCGCATCGCCGCCACCATGGAGAACGCCCAGAAGGGCGAGATCA
                     TGCCCAACATCCCCCAGATGAGCGCCTTCTGGTACGCCGTGCGCACCGCCGTGATCAA
                     CGCCGCCAGCGGCCGCCAGACCGTGGATGAGGATCTGAAGGATGCCCAGACCCGCATC
                     ACCAAGCAGTTCCGCGATCACATGGTGCTGCACGAGTACGTGAACGCCGCCGGCATCA
                     CCTAA"
     regulatory      7050..7294
                     /note="Geneious type: Terminator"
                     /standard_name="SV40_PA term"
     misc_feature    7050..7294
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="SV40_PA term (concatenated sequence 3)"
     primer_bind     complement(7274..7294)
                     /standard_name="1,415 R"
     misc_feature    7295..10390
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P859
                     p{dU6-3-GW1-Amp-ccdB-MCS-GW2-Chlor-SacB} extraction
                     (concatenated sequence 4)"
     misc_feature    complement(7295..7298)
                     /note="4 base BbsI overhang created during Golden Gate
                     assembly (BbsI site 372-377)"
                     /standard_name="BbsI overhang"
     misc_feature    7295..7298
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    complement(7299..7304)
                     /standard_name="BsaI"
     misc_feature    7298^7299
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="CGTCTCACCAT"
     misc_feature    complement(7306..7309)
                     /note="4 base BsaI overhang created during Golden Gate
                     assembly (BsaI site 395-400)"
                     /standard_name="BsaI overhang"
     misc_feature    7306
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     misc_feature    7307
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     misc_feature    7310..7433
                     /note="Geneious type: attR5"
                     /standard_name="attR5"
     misc_feature    7310..4755
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P767
                     p{dU6-3-GW1-Amp-ccdB-Mhc-GFP-FRT-P3-DsRed-GW2-Chlor-SacB}
                     (concatenated sequence 1)"
     misc_feature    7310..7313
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    7529
                     /note="Geneious type: Junction"
                     /standard_name="junction 2"
     misc_feature    7529
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     gene            7540..8199
                     /gene="CAT/CamR"
                     /standard_name="CAT/CamR"
     misc_feature    7545..8197
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AAAAAAGATTACGGGTTATACTACCGTCGACATCAGCCAGTG
                     GCACCGTAAAGAACATTTCGAAGCGTTCCAGAGCGTCGCGCAGTGCACCTACAATCAG
                     ACCGTTCAGCTGGATATCACTGCTTTCCTGAAGACTGTTAAGAAAAACAAACACAAAT
                     TTTATCCGGCTTTTATCCACATTCTCGCTCGCCTGATGAACGCACACCCGGAATTCCG
                     TATGGCCATGAAAGATGGCGAGCTGGTAATTTGGGATTCCGTTCATCCGTGTTATACC
                     GTCTTTCACGAACAGACCGAGACGTTCTCTTCCCTGTGGTCTG"
     primer_bind     complement(7638..7657)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="10,588 R"
     misc_feature    8202..10074
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CGAGACGTTCTCTTCCCTGTGGTCTGAATATCACGATGACTT
                     CCGTCAATTCCTGCACATTTATTCTCAGGACGTTGCGTGCTATGGTGAGAATCTCGCT
                     TATTTTCCGAAGGGTTTCATTGAAAACATGTTCTTTGTTAGCGCCAACCCATGGGTGA
                     GCTTCACTTCTTTCGACCTGAACGTGGCGAACATGGACAACTTTTTCGCGCCGGTTTT
                     CACGATGGGCAAGTATTACACCCAGGGTGACAAGGTACTGATGCCGCTGGCAATCCAG
                     GTGCATCACGCGGTATGTGACGGCTTCCATGTCGGTCGCATGCTGAACGAGCTGCAGC
                     AATACTGTGATGAATGGCAGGGCGGTGCGTAAACGCGTGGATCCGGCTTACTAAAAGC
                     CAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGAT
                     ATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGAC
                     AGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTC
                     TGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGC
                     GGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTT
                     GCTGACGAGAACAGGGGCTGGTGAAATGCAATTTAAAGTTTATACCTACAAGCGCGAA
                     TCTCGCTACCGTCTGTTTGTAGACGTACAGTCTGATATCATTGATACGCCGGGCCGCC
                     GTATGGTTATTCCGCTGGCATCTGCACGTCTCCTGTCTGATAAAGTTAGCCGTGAACT
                     GTATCCGGTTGTACACATTGGCGACGAGTCTTGGCGCATGATGACGACTGACATGGCT
                     TCTGTCCCGGTTAGCGTCATTGGCGAGGAAGTCGCCGATCTGTCTCACCGTGAAAATG
                     ACATCAAAAATGCAATTAACCTGATGTTCTGGGGCATTTGATCCGGCTTACTAAAAGC
                     CAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGAT
                     ATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGAC
                     AGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTC
                     TGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGC
                     GGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTT
                     GCTGACGAGAACAGGGGCTGGTGAAATGCAATTTAAAGTTTATACCTACAAGCGCGAA
                     TCTCGCTACCGTCTGTTTGTAGACGTACAGTCTGATATCATTGATACGCCGGGCCGCC
                     GTATGGTTATTCCGCTGGCATCTGCACGTCTCCTGTCTGATAAAGTTAGCCGTGAACT
                     GTATCCGGTTGTACACATTGGCGACGAGTCTTGGCGCATGATGACGACTGACATGGCT
                     TCTGTCCCGGTTAGCGTCATTGGCGAGGAAGTCGCCGATCTGTCTCACCGTGAAAATG
                     ACATCAAAAATGCAATTAACCTGATGTTCTGGGGCATTTG"
     misc_feature    8204..8207
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      8208..8653
                     /note="Geneious type: promoter"
                     /note="Bacillus subtilis sacB (glycoside hydrolase family
                     68) promoter region"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB"
     gene            8654..10077
                     /note="counterselectable marker from Bacillus subtilis
                     that converts sucrose to levans"
                     /product="levansucrase"
                     /gene="sacB"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB CS"
     misc_feature    9045..9048
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    10075
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     misc_feature    complement(10114..10237)
                     /note="Geneious type: attR6"
                     /standard_name="attR6"
     misc_feature    10238..10267
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    10238
                     /note="Geneious type: Junction"
                     /standard_name="junction 2"
     misc_feature    10240
                     /note="Geneious type: Junction"
                     /standard_name="junction 2"
     misc_feature    10268..10271
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      complement(10330..10348)
                     /note="Geneious type: Promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7 prom"
     regulatory      complement(10331..10348)
                     /regulatory_class="promoter"
                     /note="T7 promoter"
                     /standard_name="T7 promoter"
     misc_feature    10361..10390
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
ORIGIN      
        1 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
       61 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
      121 tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
      181 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
      241 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
      301 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      361 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
      421 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
      481 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
      541 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
      601 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
      661 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
      721 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
      781 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
      841 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
      901 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
      961 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     1021 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1081 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     1141 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     1201 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     1261 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     1321 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca
     1381 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1441 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1501 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1561 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1621 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1681 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1741 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1801 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1861 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1921 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1981 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2041 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2101 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2161 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa atcgtcacct
     2221 gcaggaaggt ttaaacgcat ttaggtgaca ctatagaagt gtgtatcgct cgagggatcc
     2281 gaattcgaag tcttggtacg gaggggatcc gatcgacaag ccgaacatat gggttttttg
     2341 ctcacctgtg attgctccta ctcaaataca aaaacatcaa attttctgtc aataaagcat
     2401 atttatttat atttatttta caggaaagaa ttccttttaa agtgtatttt aacctataat
     2461 gaaaaacgat taaaaaaaat acataaaata attcgaaaat ttttgaatag cccaggttga
     2521 taaaaattca tttcatacgt tttataactt atgcccctaa gtattttttg accatagtgt
     2581 ttcaattcta cattaatttt acagagtaga atgaaacgcc acctactcag ccaagaggcg
     2641 aaaaggttag ctcgccaagc agagagggcg ccagtgctca ctacttttta taattctcaa
     2701 cttctttttc cagactcagt tcgtatatat agacctattt tcaatttaac gtcggggcct
     2761 caactttgta taataaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     2821 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     2881 tatgggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
     2941 ggattttgag ttaggatccg tcgagatttt caggagctac gcggaacccc tatttgttta
     3001 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3061 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3121 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3181 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3241 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3301 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3361 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3421 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3481 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3541 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3601 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3661 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3721 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3781 tctggagccg gtgagcgtgg gtcccgcggt atcattgcag cactggggcc agatggtaag
     3841 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3901 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaatggat ccggcttact
     3961 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     4021 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     4081 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     4141 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     4201 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     4261 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     4321 tatcgtctgt ttgtggatgt acagagtgat attatcgata caccgggccg tcgcatggtt
     4381 attccacttg cgtctgcgcg cttgctctca gataaagtct cccgtgaact ttacccggtg
     4441 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtt
     4501 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     4561 attaacctga tgttctgggg aatataatgt caggctccct tatacacagc cagtctgcac
     4621 gtcgcatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     4681 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa cttttctata
     4741 caaagttgtc cagctggagc gagaccggag actagttgtt cgccgagctc ttacgcgccc
     4801 gcgtaaaaca caatcaagta tgagtcataa gctgatgtca tgttttgcac acggctcata
     4861 accgaactgg ctttacgagt agaattctac ttgtaacgca cgatcagtgg atgatgtcat
     4921 ttgtttttca aatcgagatg atgtcatgtt ttgcacacgg ctcataaact cgctttacga
     4981 gtagaattct acgtgtaacg cacgatcgat tgatgagtca tttgttttgc aatatgatat
     5041 catacaatat gactcatttg tttttcaaaa ccgaacttga tttacgggta gaattctact
     5101 tgtaaagcac aatcaaaaag atgatgtcat ttgtttttca aaactgaact cgctttacga
     5161 gtagaattct acgtgtaaaa cacaatcaag aaatgatgtc atttgttata aaaataaaag
     5221 ctgatgtcat gttttgcaca tggctcataa ctaaactcgc tttacgggta gaattctacg
     5281 cgcgtcgatg tctttgtgat gcgcgcgaca tttttgtagg ttattgataa aatgaacgga
     5341 tacgttgccc gacattatca ttaaatcctt ggcgtagaat ttgtcgggtc cattgtccgt
     5401 gtgcgctagc atgcccgtaa cggacctcgt acttttggct tcaaaggttt tgcgcacaga
     5461 caaaatgtgc cacacttgca gctctgcatg tgtgcgcgtt accacaaatc ccaacggcgc
     5521 agtgtacttg ttgtatgcaa ataaatctcg ataaaggcgc ggcgcgcgaa tgcagctgat
     5581 cacgtacgct cctcgtgttc cgttcaagga cggtgttatc gacctcagat taatgtttat
     5641 cggccgactg ttttcgtatc cgctcaccaa acgcgttttt gcattaacat tgtatgtcgg
     5701 cggatgttct atatctaatt tgaataaata aacgataacc gcgttggttt tagagggcat
     5761 aataaaagaa atattgttat cgtgttcgcc attagggcag tataaattga cgttcatgtt
     5821 ggatattgtt tcagttgcaa gttgacactg gcggcgacaa gatcgtgaac aaccaagtga
     5881 ccatgaagat cgaggagggc aagttggtca tctggattaa tggagataag ggctataatg
     5941 gactggcaga ggttggaaaa aaatttgaga aggatacagg cattaaggtc acagttgagc
     6001 atccagataa gttggaggag aagttcccgc aggtggctgc gacgggtgac ggacccgaca
     6061 tcatattctg ggctcatgac cgtttcggag gatacgccca gtccggcttg ttggcggaaa
     6121 ttacccctga taaagctttt caggacaaac tctacccctt cacctgggat gctgtccgat
     6181 acaacggcaa gctcattgca taccccattg cagttgaagc actgagtctg atttacaata
     6241 aagacctgtt gccgaatcct ccgaagacct gggaggagat tcccgctctg gacaaggagc
     6301 tgaaggctaa gggcaagtcc gccctgatgt tcaacctgca agagccgtac tttacttggc
     6361 ccctgatcgc agccgacggc ggatacgcct tcaaatacga gaacggaaag tacgatataa
     6421 aggatgtcgg agtcgacaac gccggcgcta aagcaggcct gactttcctg gtggacctca
     6481 taaagaacaa gcatatgaat gccgacaccg attacagtat agcggaagcc gcgttcaaca
     6541 agggcgagac tgccatgaca ataaacggac cctgggcatg gtccaacatt gacacgagca
     6601 aggtgaacta cggtgtaacg gtgttgccta cgttcaaggg acagccgagt aaaccattcg
     6661 ttggcgtttt gtcggctgga attaatgctg ctagtcccaa taaggagttg gctaaggagt
     6721 tcttggagaa ctacttgctg acagacgagg gcttggaggc agtaaacaag gataagcccc
     6781 tgggtgcggt tgccctcaag tcctatgaag aagagctcgt aaaagacccg cgtattgctg
     6841 cgaccatgga gaatgcccag aagggtgaga ttatgcccaa tataccccag atgtccgcgt
     6901 tttggtatgc agttcgcacc gcggttataa acgcagcctc gggacggcag accgtggatg
     6961 aggacttgaa agacgcccag acacgcataa ccaaacagtt tcgcgaccat atggttctgc
     7021 atgaatatgt caacgcagca ggcattacta gatcataatc agccatacca catttgtaga
     7081 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     7141 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7201 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7261 actcatcaat gtatcttaaa gcttatcgat acgcccatgg tctcaccatc aactttgtat
     7321 acaaaagttg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     7381 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atgggccgca
     7441 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt
     7501 taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
     7561 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
     7621 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
     7681 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
     7741 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt
     7801 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
     7861 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
     7921 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtgtcagcc
     7981 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
     8041 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
     8101 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa
     8161 ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggcac atatacctgc
     8221 cgttcactat tatttagtga aatgagatat tatgatattt tctgaattgt gattaaaaag
     8281 gcaactttat gcccatgcaa cagaaactat aaaaaataca gagaatgaaa agaaacagat
     8341 agatttttta gttctttagg cccgtagtct gcaaatcctt ttatgatttt ctatcaaaca
     8401 aaagaggaaa atagaccagt tgcaatccaa acgagagtct aatagaatga ggtcgaaaag
     8461 taaatcgcgc gggtttgtta ctgataaagc aggcaagacc taaaatgtgt aaagggcaaa
     8521 gtgtatactt tggcgtcacc ccttacatat tttaggtctt tttttattgt gcgtaactaa
     8581 cttgccatct tcaaacagga gggctggaag aagcagaccg ctaacacagt acataaaaaa
     8641 ggagacatga acgatgaaca tcaaaaagtt tgcaaaacaa gcaacagtat taacctttac
     8701 taccgcactg ctggcaggag gcgcaactca agcgtttgcg aaagaaacga accaaaagcc
     8761 atataaggaa acatacggca tttcccatat tacacgccat gatatgctgc aaatccctga
     8821 acagcaaaaa aatgaaaaat atcaagttcc tgaattcgat tcgtccacaa ttaaaaatat
     8881 ctcttctgca aaaggcctgg acgtttggga cagctggcca ttacaaaacg ctgacggcac
     8941 tgtcgcaaac tatcacggct accacatcgt ctttgcatta gccggagatc ctaaaaatgc
     9001 ggatgacaca tcgatttaca tgttctatca aaaagtcggc gaaacttcta ttgacagctg
     9061 gaaaaacgct ggccgcgtct ttaaagacag cgacaaattc gatgcaaatg attctatcct
     9121 aaaagaccaa acacaagaat ggtcaggttc agccacattt acatctgacg gaaaaatccg
     9181 tttattctac actgatttct ccggtaaaca ttacggcaaa caaacactga caactgcaca
     9241 agttaacgta tcagcatcag acagctcttt gaacatcaac ggtgtagagg attataaatc
     9301 aatctttgac ggtgacggaa aaacgtatca aaatgtacag cagttcatcg atgaaggcaa
     9361 ctacagctca ggcgacaacc atacgctgag agatcctcac tacgtagaag ataaaggcca
     9421 caaatactta gtatttgaag caaacactgg aactgaagat ggctaccaag gcgaagaatc
     9481 tttatttaac aaagcatact atggcaaaag cacatcattc ttccgtcaag aaagtcaaaa
     9541 acttctgcaa agcgataaaa aacgcacggc tgagttagca aacggcgctc tcggtatgat
     9601 tgagctaaac gatgattaca cactgaaaaa agtgatgaaa ccgctgattg catctaacac
     9661 agtaacagat gaaattgaac gcgcgaacgt ctttaaaatg aacggcaaat ggtacctgtt
     9721 cactgactcc cgcggatcaa aaatgacgat tgacggcatt acgtctaacg atatttacat
     9781 gcttggttat gtttctaatt ctttaactgg cccatacaag ccgctgaaca aaactggcct
     9841 tgtgttaaaa atggatcttg atcctaacga tgtaaccttt acttactcac acttcgctgt
     9901 acctcaagcg aaaggaaaca atgtcgtgat tacaagctat atgacaaaca gaggattcta
     9961 cgcagacaaa caatcaacgt ttgcgccaag cttcctgctg aacatcaaag gcaagaaaac
    10021 atctgttgtc aaagacagca tccttgaaca aggacaatta acagttaaca aataaatgtc
    10081 aggctccctt atacacagcc agtctgcacg tcgcatagtg actggatatg ttgtgtttta
    10141 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
    10201 ttttacgttt ctcgttcaac tttttaatac aaagttgcct ccggaacata atggtgcatc
    10261 tagatgccca ttcctgtaga cttcttaatt aagacgtcag aattctcgag gcggccgcat
    10321 gtgagtctcc ctatagtgag tcgtattaat ttcgcgggcg cgtcagcggg agagccgttg
    10381 taaggcggca
//
