LOCUS       pJAT22{dU6-3-attR3-Amp-ccdB-attR4-3XP3-GFPn-attR5-Chlor-SacB-attR6}        8987 bp    DNA     circular UNA 12-JUN-2023
DEFINITION  Golden Gate/Type IIS Cloning ligation product.
ACCESSION   urn.local.sternd.22b-gdru7wy
VERSION     urn.local.sternd.22b-gdru7wy
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..4770
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P859
                     p{dU6-3-GW1-Amp-ccdB-MCS-GW2-Chlor-SacB} extraction
                     (concatenated sequence 1)"
     CDS             411..1202
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /standard_name="SmR"
     rep_origin      1448..2036
     misc_feature    1820..1837
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ori-252 R"
     primer_bind     1937..1956
                     /note="pBR322 origin, forward primer"
                     /standard_name="pBR322ori-F"
     primer_bind     2026..2045
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7Cas9F"
     primer_bind     complement(2052..2071)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp-ori-7571F"
     misc_feature    2180..2209
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     2190..2207
                     /note="L4440 vector, forward primer"
                     /standard_name="L4440"
     regulatory      2239..2258
                     /note="Geneious type: promoter"
                     /note="SP6 RNA polymerase promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SP6"
     misc_feature    2300..2303
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2304..2333
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     regulatory      2334..2754
                     /note="Geneious type: promoter"
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
                     /standard_name="dU6-3 promoter"
     misc_feature    complement(2399..2409)
                     /note="Geneious type: Transcription Factor"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Hb"
     misc_feature    2755..2787
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    2756
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     misc_feature    2757
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     misc_feature    2758..2787
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2761..2884
                     /note="Geneious type: attR3"
                     /standard_name="attR3"
     regulatory      2907..2937
                     /note="Geneious type: promoter"
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacUV5"
     misc_feature    complement(2918..2929)
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     misc_feature    2976..2979
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2979
                     /note="Geneious type: Junction"
                     /standard_name="junction 2"
     misc_feature    2979
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     regulatory      2980..3084
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     gene            3085..3945
                     /standard_name="AmpR"
     primer_bind     complement(3125..3146)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AMP_5'_F"
     misc_feature    3331..3350
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_R"
     misc_feature    3804
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="T"
     primer_bind     complement(3817..3836)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_F"
     misc_feature    3909..3932
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp_3'_R"
     primer_bind     complement(3922..3945)
                     /standard_name="966 R"
     misc_feature    3942..3945
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    3946..4585
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGT
                     TGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAAT
                     GAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
                     AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTG
                     AAATGCAATTTAAGGTTTATACTTACAAGCGCGAGTCCCGCTATCGCCTTTTCGTGGA
                     CGTCCAGTCAGACATTATCGACACGCCTGGTCGCCGTATGGTGATTCCATTAGCCAGC
                     GCACGCCTGTTAAGTGATAAGGTGTCGCGCGAGCTTTACCCAGTCGTCCATATTGGTG
                     ACGAGAGTTGGCGCATGATGACTACAGACATGGCTAGTGTACCTGTGTCGGTGATCGG
                     GGAAGAGGTCGCGGATTTAAGCCACCGTGAGAATGATATTAAGAATGCAATCAATCTC
                     ATGTTTTGGGGTATTTA"
     primer_bind     3946..3967
                     /standard_name="1 F"
     gene            4255..4587
                     /standard_name="ccdB"
     primer_bind     4446..4465
                     /standard_name="4446F"
     misc_feature    4585
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     misc_feature    complement(4625..4748)
                     /note="Geneious type: attR4"
                     /standard_name="attR4"
     primer_bind     4741..4760
                     /standard_name="4741F"
     misc_feature    4748
                     /note="Geneious type: Junction"
                     /standard_name="junction 2"
     misc_feature    4750
                     /note="Geneious type: Junction"
                     /standard_name="junction 2"
     misc_feature    join(4752..>4770,<5892..5910)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pGGAselect-Spect extraction (concatenated
                     sequence 2)"
     misc_feature    4752..4755
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    join(<4756..>4770,<5892..>5906)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pGGAselect PCR Product (concatenated
                     sequence 2)"
     misc_feature    4756..4759
                     /note="4 base BsaI overhang created during Golden Gate
                     assembly (BsaI site 320-325)"
                     /standard_name="BsaI overhang"
     misc_feature    join(4760..>4770,<5892..5902)
                     /note="DNA sequence removed and replaced during Golden
                     Gate assembly with BsaI"
                     /standard_name="Fragment removed by BsaI"
     misc_feature    4761..4766
                     /standard_name="BsaI"
     misc_feature    4767..5895
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="3XP3-GFPn-SV40 (concatenated sequence 2)"
     misc_feature    4767..4770
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    4767..4770
                     /note="4 base BbsI overhang created during Golden Gate
                     assembly (BbsI site 343-348)"
                     /standard_name="BbsI overhang"
     misc_feature    4771..4795
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     primer_bind     4771..4792
                     /standard_name="1 F"
     misc_feature    4796..5001
                     /standard_name="3xP3-hsp70"
     misc_feature    4796..5001
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="3xP3-hsp70 (concatenated sequence 1)"
     misc_feature    5002..5646
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="GFPn (back translated) (concatenated
                     sequence 2)"
     regulatory      5647..5891
                     /note="Geneious type: Terminator"
                     /standard_name="SV40_PA term"
     misc_feature    5647..5891
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="SV40_PA term (concatenated sequence 3)"
     primer_bind     complement(5871..5891)
                     /standard_name="1,121 R"
     misc_feature    5892..5895
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    5892..8987
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P859
                     p{dU6-3-GW1-Amp-ccdB-MCS-GW2-Chlor-SacB} extraction
                     (concatenated sequence 3)"
     misc_feature    complement(5892..5895)
                     /note="4 base BbsI overhang created during Golden Gate
                     assembly (BbsI site 372-377)"
                     /standard_name="BbsI overhang"
     misc_feature    5895^5896
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="CGTCTCACCAT"
     misc_feature    complement(5896..5901)
                     /standard_name="BsaI"
     misc_feature    complement(5903..5906)
                     /note="4 base BsaI overhang created during Golden Gate
                     assembly (BsaI site 395-400)"
                     /standard_name="BsaI overhang"
     misc_feature    5903
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     misc_feature    5904
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     misc_feature    5907..6030
                     /note="Geneious type: attR5"
                     /standard_name="attR5"
     misc_feature    5907..4755
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P767
                     p{dU6-3-GW1-Amp-ccdB-Mhc-GFP-FRT-P3-DsRed-GW2-Chlor-SacB}
                     (concatenated sequence 1)"
     misc_feature    5907..5910
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    6126
                     /note="Geneious type: Junction"
                     /standard_name="junction 2"
     misc_feature    6126
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     gene            6137..6796
                     /gene="CAT/CamR"
                     /standard_name="CAT/CamR"
     misc_feature    6142..6794
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AAAAAAGATTACGGGTTATACTACCGTCGACATCAGCCAGTG
                     GCACCGTAAAGAACATTTCGAAGCGTTCCAGAGCGTCGCGCAGTGCACCTACAATCAG
                     ACCGTTCAGCTGGATATCACTGCTTTCCTGAAGACTGTTAAGAAAAACAAACACAAAT
                     TTTATCCGGCTTTTATCCACATTCTCGCTCGCCTGATGAACGCACACCCGGAATTCCG
                     TATGGCCATGAAAGATGGCGAGCTGGTAATTTGGGATTCCGTTCATCCGTGTTATACC
                     GTCTTTCACGAACAGACCGAGACGTTCTCTTCCCTGTGGTCTG"
     primer_bind     complement(6235..6254)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="10,588 R"
     misc_feature    6799..8671
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CGAGACGTTCTCTTCCCTGTGGTCTGAATATCACGATGACTT
                     CCGTCAATTCCTGCACATTTATTCTCAGGACGTTGCGTGCTATGGTGAGAATCTCGCT
                     TATTTTCCGAAGGGTTTCATTGAAAACATGTTCTTTGTTAGCGCCAACCCATGGGTGA
                     GCTTCACTTCTTTCGACCTGAACGTGGCGAACATGGACAACTTTTTCGCGCCGGTTTT
                     CACGATGGGCAAGTATTACACCCAGGGTGACAAGGTACTGATGCCGCTGGCAATCCAG
                     GTGCATCACGCGGTATGTGACGGCTTCCATGTCGGTCGCATGCTGAACGAGCTGCAGC
                     AATACTGTGATGAATGGCAGGGCGGTGCGTAAACGCGTGGATCCGGCTTACTAAAAGC
                     CAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGAT
                     ATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGAC
                     AGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTC
                     TGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGC
                     GGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTT
                     GCTGACGAGAACAGGGGCTGGTGAAATGCAATTTAAAGTTTATACCTACAAGCGCGAA
                     TCTCGCTACCGTCTGTTTGTAGACGTACAGTCTGATATCATTGATACGCCGGGCCGCC
                     GTATGGTTATTCCGCTGGCATCTGCACGTCTCCTGTCTGATAAAGTTAGCCGTGAACT
                     GTATCCGGTTGTACACATTGGCGACGAGTCTTGGCGCATGATGACGACTGACATGGCT
                     TCTGTCCCGGTTAGCGTCATTGGCGAGGAAGTCGCCGATCTGTCTCACCGTGAAAATG
                     ACATCAAAAATGCAATTAACCTGATGTTCTGGGGCATTTGATCCGGCTTACTAAAAGC
                     CAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGAT
                     ATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGAC
                     AGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTC
                     TGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGC
                     GGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTT
                     GCTGACGAGAACAGGGGCTGGTGAAATGCAATTTAAAGTTTATACCTACAAGCGCGAA
                     TCTCGCTACCGTCTGTTTGTAGACGTACAGTCTGATATCATTGATACGCCGGGCCGCC
                     GTATGGTTATTCCGCTGGCATCTGCACGTCTCCTGTCTGATAAAGTTAGCCGTGAACT
                     GTATCCGGTTGTACACATTGGCGACGAGTCTTGGCGCATGATGACGACTGACATGGCT
                     TCTGTCCCGGTTAGCGTCATTGGCGAGGAAGTCGCCGATCTGTCTCACCGTGAAAATG
                     ACATCAAAAATGCAATTAACCTGATGTTCTGGGGCATTTG"
     misc_feature    6801..6804
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      6805..7250
                     /note="Geneious type: promoter"
                     /note="Bacillus subtilis sacB (glycoside hydrolase family
                     68) promoter region"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB"
     gene            7251..8674
                     /note="counterselectable marker from Bacillus subtilis
                     that converts sucrose to levans"
                     /product="levansucrase"
                     /gene="sacB"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB CS"
     misc_feature    7642..7645
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    8672
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     misc_feature    complement(8711..8834)
                     /note="Geneious type: attR6"
                     /standard_name="attR6"
     misc_feature    8835..8864
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    8835
                     /note="Geneious type: Junction"
                     /standard_name="junction 2"
     misc_feature    8837
                     /note="Geneious type: Junction"
                     /standard_name="junction 2"
     misc_feature    8865..8868
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      complement(8927..8945)
                     /note="Geneious type: Promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7 prom"
     regulatory      complement(8928..8945)
                     /regulatory_class="promoter"
                     /note="T7 promoter"
                     /standard_name="T7 promoter"
     misc_feature    8958..8987
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
ORIGIN      
        1 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
       61 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
      121 tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
      181 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
      241 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
      301 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      361 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
      421 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
      481 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
      541 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
      601 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
      661 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
      721 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
      781 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
      841 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
      901 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
      961 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     1021 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1081 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     1141 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     1201 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     1261 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     1321 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca
     1381 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1441 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1501 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1561 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1621 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1681 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1741 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1801 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1861 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1921 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1981 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2041 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2101 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2161 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa atcgtcacct
     2221 gcaggaaggt ttaaacgcat ttaggtgaca ctatagaagt gtgtatcgct cgagggatcc
     2281 gaattcgaag tcttggtacg gaggggatcc gatcgacaag ccgaacatat gggttttttg
     2341 ctcacctgtg attgctccta ctcaaataca aaaacatcaa attttctgtc aataaagcat
     2401 atttatttat atttatttta caggaaagaa ttccttttaa agtgtatttt aacctataat
     2461 gaaaaacgat taaaaaaaat acataaaata attcgaaaat ttttgaatag cccaggttga
     2521 taaaaattca tttcatacgt tttataactt atgcccctaa gtattttttg accatagtgt
     2581 ttcaattcta cattaatttt acagagtaga atgaaacgcc acctactcag ccaagaggcg
     2641 aaaaggttag ctcgccaagc agagagggcg ccagtgctca ctacttttta taattctcaa
     2701 cttctttttc cagactcagt tcgtatatat agacctattt tcaatttaac gtcggggcct
     2761 caactttgta taataaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     2821 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     2881 tatgggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
     2941 ggattttgag ttaggatccg tcgagatttt caggagctac gcggaacccc tatttgttta
     3001 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3061 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3121 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3181 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3241 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3301 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3361 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3421 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3481 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3541 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3601 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3661 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3721 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3781 tctggagccg gtgagcgtgg gtcccgcggt atcattgcag cactggggcc agatggtaag
     3841 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3901 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaatggat ccggcttact
     3961 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     4021 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     4081 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     4141 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     4201 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     4261 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     4321 tatcgtctgt ttgtggatgt acagagtgat attatcgata caccgggccg tcgcatggtt
     4381 attccacttg cgtctgcgcg cttgctctca gataaagtct cccgtgaact ttacccggtg
     4441 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtt
     4501 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     4561 attaacctga tgttctgggg aatataatgt caggctccct tatacacagc cagtctgcac
     4621 gtcgcatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     4681 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa cttttctata
     4741 caaagttgtc cagctggagc gagaccggag caatggttaa ttcgagctcg cccggggatc
     4801 taattcaatt agagactaat tcaattagag ctaattcaat taggatccaa gcttatcgat
     4861 ttcgaaccct cgaccgccgg agtataaata gaggcgcttc gtctacggag cgacaattca
     4921 attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa acaagcgcag
     4981 ctgaacaagc taaacaatcg gatgagcaag ggcgaggagc tgttcaccgg cgtggtgccc
     5041 atcctggtgg agctggatgg cgatgtgaac ggccacaagt tcagcgtgcg cggcgagggc
     5101 gagggcgatg ccaccatcgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     5161 cccgtgccct ggcccaccct ggtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     5221 taccccgatc acatgaagcg ccacgatttc ttcaagagcg ccatgcccga gggctacgtg
     5281 caggagcgca ccatcagctt caaggatgat ggcaagtaca agacccgcgc cgtggtgaag
     5341 ttcgagggcg ataccctggt gaaccgcatc gagctgaagg gcaccgattt caaggaggat
     5401 ggcaacatcc tgggccacaa gctggagtac aacttcaaca gccacaacgt gtacatcacc
     5461 gccgataagc agaagaacgg catcaaggcc aacttcaccg tgcgccacaa cgtggaggat
     5521 ggcagcgtgc agctggccga tcactaccag cagaacaccc ccatcggcga tggccccgtg
     5581 ctgctgcccg ataaccacta cctgagcacc cagaccgtgc tgagcaagga tcccaacgag
     5641 aagtaaagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     5701 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     5761 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5821 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaagct
     5881 tatcgatacg cccatggtct caccatcaac tttgtataca aaagttgaac gagaaacgta
     5941 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
     6001 ctgtaaaaca caacatatcc agtcactatg ggccgcatta ggcaccccag gctttacact
     6061 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg
     6121 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     6181 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     6241 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     6301 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
     6361 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     6421 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     6481 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     6541 taaagggttt attgagaata tgtttttcgt gtcagccaat ccctgggtga gtttcaccag
     6601 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     6661 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     6721 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     6781 gcagggcggg gcgtaaacgc gtggcacata tacctgccgt tcactattat ttagtgaaat
     6841 gagatattat gatattttct gaattgtgat taaaaaggca actttatgcc catgcaacag
     6901 aaactataaa aaatacagag aatgaaaaga aacagataga ttttttagtt ctttaggccc
     6961 gtagtctgca aatcctttta tgattttcta tcaaacaaaa gaggaaaata gaccagttgc
     7021 aatccaaacg agagtctaat agaatgaggt cgaaaagtaa atcgcgcggg tttgttactg
     7081 ataaagcagg caagacctaa aatgtgtaaa gggcaaagtg tatactttgg cgtcacccct
     7141 tacatatttt aggtcttttt ttattgtgcg taactaactt gccatcttca aacaggaggg
     7201 ctggaagaag cagaccgcta acacagtaca taaaaaagga gacatgaacg atgaacatca
     7261 aaaagtttgc aaaacaagca acagtattaa cctttactac cgcactgctg gcaggaggcg
     7321 caactcaagc gtttgcgaaa gaaacgaacc aaaagccata taaggaaaca tacggcattt
     7381 cccatattac acgccatgat atgctgcaaa tccctgaaca gcaaaaaaat gaaaaatatc
     7441 aagttcctga attcgattcg tccacaatta aaaatatctc ttctgcaaaa ggcctggacg
     7501 tttgggacag ctggccatta caaaacgctg acggcactgt cgcaaactat cacggctacc
     7561 acatcgtctt tgcattagcc ggagatccta aaaatgcgga tgacacatcg atttacatgt
     7621 tctatcaaaa agtcggcgaa acttctattg acagctggaa aaacgctggc cgcgtcttta
     7681 aagacagcga caaattcgat gcaaatgatt ctatcctaaa agaccaaaca caagaatggt
     7741 caggttcagc cacatttaca tctgacggaa aaatccgttt attctacact gatttctccg
     7801 gtaaacatta cggcaaacaa acactgacaa ctgcacaagt taacgtatca gcatcagaca
     7861 gctctttgaa catcaacggt gtagaggatt ataaatcaat ctttgacggt gacggaaaaa
     7921 cgtatcaaaa tgtacagcag ttcatcgatg aaggcaacta cagctcaggc gacaaccata
     7981 cgctgagaga tcctcactac gtagaagata aaggccacaa atacttagta tttgaagcaa
     8041 acactggaac tgaagatggc taccaaggcg aagaatcttt atttaacaaa gcatactatg
     8101 gcaaaagcac atcattcttc cgtcaagaaa gtcaaaaact tctgcaaagc gataaaaaac
     8161 gcacggctga gttagcaaac ggcgctctcg gtatgattga gctaaacgat gattacacac
     8221 tgaaaaaagt gatgaaaccg ctgattgcat ctaacacagt aacagatgaa attgaacgcg
     8281 cgaacgtctt taaaatgaac ggcaaatggt acctgttcac tgactcccgc ggatcaaaaa
     8341 tgacgattga cggcattacg tctaacgata tttacatgct tggttatgtt tctaattctt
     8401 taactggccc atacaagccg ctgaacaaaa ctggccttgt gttaaaaatg gatcttgatc
     8461 ctaacgatgt aacctttact tactcacact tcgctgtacc tcaagcgaaa ggaaacaatg
     8521 tcgtgattac aagctatatg acaaacagag gattctacgc agacaaacaa tcaacgtttg
     8581 cgccaagctt cctgctgaac atcaaaggca agaaaacatc tgttgtcaaa gacagcatcc
     8641 ttgaacaagg acaattaaca gttaacaaat aaatgtcagg ctcccttata cacagccagt
     8701 ctgcacgtcg catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     8761 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt
     8821 ttaatacaaa gttgcctccg gaacataatg gtgcatctag atgcccattc ctgtagactt
     8881 cttaattaag acgtcagaat tctcgaggcg gccgcatgtg agtctcccta tagtgagtcg
     8941 tattaatttc gcgggcgcgt cagcgggaga gccgttgtaa ggcggca
//
