LOCUS       pJAT23{dU6-3-attR3-Amp-ccdB-attR4-3XP3-MBP-GFPc-attR5-Chlor-SacB-attR6}        9512 bp    DNA     circular UNA 12-JUN-2023
DEFINITION  Golden Gate/Type IIS Cloning ligation product.
ACCESSION   urn.local.sternd.22l-gdru89o
VERSION     urn.local.sternd.22l-gdru89o
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..4770
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P859
                     p{dU6-3-GW1-Amp-ccdB-MCS-GW2-Chlor-SacB} extraction
                     (concatenated sequence 1)"
     CDS             411..1202
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /standard_name="SmR"
     rep_origin      1448..2036
     misc_feature    1820..1837
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ori-252 R"
     primer_bind     1937..1956
                     /note="pBR322 origin, forward primer"
                     /standard_name="pBR322ori-F"
     primer_bind     2026..2045
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7Cas9F"
     primer_bind     complement(2052..2071)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp-ori-7571F"
     misc_feature    2180..2209
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     2190..2207
                     /note="L4440 vector, forward primer"
                     /standard_name="L4440"
     regulatory      2239..2258
                     /note="Geneious type: promoter"
                     /note="SP6 RNA polymerase promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SP6"
     misc_feature    2300..2303
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2304..2333
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     regulatory      2334..2754
                     /note="Geneious type: promoter"
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
                     /standard_name="dU6-3 promoter"
     misc_feature    complement(2399..2409)
                     /note="Geneious type: Transcription Factor"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Hb"
     misc_feature    2755..2787
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    2756
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     misc_feature    2757
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     misc_feature    2758..2787
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2761..2884
                     /note="Geneious type: attR3"
                     /standard_name="attR3"
     regulatory      2907..2937
                     /note="Geneious type: promoter"
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacUV5"
     misc_feature    complement(2918..2929)
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     misc_feature    2976..2979
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2979
                     /note="Geneious type: Junction"
                     /standard_name="junction 2"
     misc_feature    2979
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     regulatory      2980..3084
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     gene            3085..3945
                     /standard_name="AmpR"
     primer_bind     complement(3125..3146)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AMP_5'_F"
     misc_feature    3331..3350
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_R"
     misc_feature    3804
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="T"
     primer_bind     complement(3817..3836)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp+_F"
     misc_feature    3909..3932
                     /note="Geneious type: primer_bind_reverse"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp_3'_R"
     primer_bind     complement(3922..3945)
                     /standard_name="966 R"
     misc_feature    3942..3945
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     3946..3967
                     /standard_name="1 F"
     misc_feature    3946..4585
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGT
                     TGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAAT
                     GAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
                     AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTG
                     AAATGCAATTTAAGGTTTATACTTACAAGCGCGAGTCCCGCTATCGCCTTTTCGTGGA
                     CGTCCAGTCAGACATTATCGACACGCCTGGTCGCCGTATGGTGATTCCATTAGCCAGC
                     GCACGCCTGTTAAGTGATAAGGTGTCGCGCGAGCTTTACCCAGTCGTCCATATTGGTG
                     ACGAGAGTTGGCGCATGATGACTACAGACATGGCTAGTGTACCTGTGTCGGTGATCGG
                     GGAAGAGGTCGCGGATTTAAGCCACCGTGAGAATGATATTAAGAATGCAATCAATCTC
                     ATGTTTTGGGGTATTTA"
     gene            4255..4587
                     /standard_name="ccdB"
     primer_bind     4446..4465
                     /standard_name="4446F"
     misc_feature    4585
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     misc_feature    complement(4625..4748)
                     /note="Geneious type: attR4"
                     /standard_name="attR4"
     primer_bind     4741..4760
                     /standard_name="4741F"
     misc_feature    4748
                     /note="Geneious type: Junction"
                     /standard_name="junction 2"
     misc_feature    4750
                     /note="Geneious type: Junction"
                     /standard_name="junction 2"
     misc_feature    join(4752..>4770,<6417..6435)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pGGAselect-Spect extraction (concatenated
                     sequence 2)"
     misc_feature    4752..4755
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    join(<4756..>4770,<6417..>6431)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pGGAselect PCR Product (concatenated
                     sequence 2)"
     misc_feature    4756..4759
                     /note="4 base BsaI overhang created during Golden Gate
                     assembly (BsaI site 320-325)"
                     /standard_name="BsaI overhang"
     misc_feature    join(4760..>4770,<6417..6427)
                     /note="DNA sequence removed and replaced during Golden
                     Gate assembly with BsaI"
                     /standard_name="Fragment removed by BsaI"
     misc_feature    4761..4766
                     /standard_name="BsaI"
     misc_feature    4767..6420
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="3XP3-MBP-GFPc-SV40 (concatenated sequence
                     2)"
     misc_feature    4767..4770
                     /note="4 base BbsI overhang created during Golden Gate
                     assembly (BbsI site 343-348)"
                     /standard_name="BbsI overhang"
     misc_feature    4767..4770
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     4771..4792
                     /standard_name="1 F"
     misc_feature    4771..4795
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    4796..5001
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="3xP3-hsp70 (concatenated sequence 1)"
     misc_feature    4796..5001
                     /standard_name="3xP3-hsp70"
     misc_feature    4800..4812
                     /note="Geneious type: dna binding region"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="P3"
     misc_feature    4816..4828
                     /note="Geneious type: dna binding region"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="P3"
     misc_feature    4831..4843
                     /note="Geneious type: dna binding region"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="P3"
     gene            5002..6171
                     /standard_name="MBP-GFPc"
     misc_feature    5007..6168
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="GATCGAGGAGGGCAAGCTGGTGATCTGGATCAACGGCGATAA
                     GGGCTACAACGGCCTGGCCGAGGTGGGCAAGAAGTTCGAGAAGGATACCGGCATCAAG
                     GTGACCGTGGAGCACCCCGATAAGCTGGAGGAGAAGTTCCCCCAGGTGGCCGCCACCG
                     GCGATGGCCCCGATATCATCTTCTGGGCCCACGATCGCTTCGGCGGCTACGCCCAGAG
                     CGGCCTGCTGGCCGAGATCACCCCCGATAAGGCCTTCCAGGATAAGCTGTACCCCTTC
                     ACCTGGGATGCCGTGCGCTACAACGGCAAGCTGATCGCCTACCCCATCGCCGTGGAGG
                     CCCTGAGCCTGATCTACAACAAGGATCTGCTGCCCAACCCCCCCAAGACCTGGGAGGA
                     GATCCCCGCCCTGGATAAGGAGCTGAAGGCCAAGGGCAAGAGCGCCCTGATGTTCAAC
                     CTGCAGGAGCCCTACTTCACCTGGCCCCTGATCGCCGCCGATGGCGGCTACGCCTTCA
                     AGTACGAGAACGGCAAGTACGATATCAAGGATGTGGGCGTGGATAACGCCGGCGCCAA
                     GGCCGGCCTGACCTTCCTGGTGGATCTGATCAAGAACAAGCACATGAACGCCGATACC
                     GATTACAGCATCGCCGAGGCCGCCTTCAACAAGGGCGAGACCGCCATGACCATCAACG
                     GCCCCTGGGCCTGGAGCAACATCGATACCAGCAAGGTGAACTACGGCGTGACCGTGCT
                     GCCCACCTTCAAGGGCCAGCCCAGCAAGCCCTTCGTGGGCGTGCTGAGCGCCGGCATC
                     AACGCCGCCAGCCCCAACAAGGAGCTGGCCAAGGAGTTCCTGGAGAACTACCTGCTGA
                     CCGATGAGGGCCTGGAGGCCGTGAACAAGGATAAGCCCCTGGGCGCCGTGGCCCTGAA
                     GAGCTACGAGGAGGAGCTGGTGAAGGATCCCCGCATCGCCGCCACCATGGAGAACGCC
                     CAGAAGGGCGAGATCATGCCCAACATCCCCCAGATGAGCGCCTTCTGGTACGCCGTGC
                     GCACCGCCGTGATCAACGCCGCCAGCGGCCGCCAGACCGTGGATGAGGATCTGAAGGA
                     TGCCCAGACCCGCATCACCAAGCAGTTCCGCGATCACATGGTGCTGCACGAGTACGTG
                     AACGCCGCCGGCATCACC"
     regulatory      6172..6416
                     /note="Geneious type: Terminator"
                     /standard_name="SV40_PA term"
     misc_feature    6172..6416
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="SV40_PA term (concatenated sequence 3)"
     primer_bind     complement(6396..6416)
                     /standard_name="1,646 R"
     misc_feature    6417..9512
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P859
                     p{dU6-3-GW1-Amp-ccdB-MCS-GW2-Chlor-SacB} extraction
                     (concatenated sequence 3)"
     misc_feature    complement(6417..6420)
                     /note="4 base BbsI overhang created during Golden Gate
                     assembly (BbsI site 372-377)"
                     /standard_name="BbsI overhang"
     misc_feature    6417..6420
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    complement(6421..6426)
                     /standard_name="BsaI"
     misc_feature    6420^6421
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="CGTCTCACCAT"
     misc_feature    complement(6428..6431)
                     /note="4 base BsaI overhang created during Golden Gate
                     assembly (BsaI site 395-400)"
                     /standard_name="BsaI overhang"
     misc_feature    6428
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     misc_feature    6429
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     misc_feature    6432..6555
                     /note="Geneious type: attR5"
                     /standard_name="attR5"
     misc_feature    6432..4755
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="P767
                     p{dU6-3-GW1-Amp-ccdB-Mhc-GFP-FRT-P3-DsRed-GW2-Chlor-SacB}
                     (concatenated sequence 1)"
     misc_feature    6432..6435
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    6651
                     /note="Geneious type: Junction"
                     /standard_name="junction 2"
     misc_feature    6651
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     gene            6662..7321
                     /gene="CAT/CamR"
                     /standard_name="CAT/CamR"
     misc_feature    6667..7319
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AAAAAAGATTACGGGTTATACTACCGTCGACATCAGCCAGTG
                     GCACCGTAAAGAACATTTCGAAGCGTTCCAGAGCGTCGCGCAGTGCACCTACAATCAG
                     ACCGTTCAGCTGGATATCACTGCTTTCCTGAAGACTGTTAAGAAAAACAAACACAAAT
                     TTTATCCGGCTTTTATCCACATTCTCGCTCGCCTGATGAACGCACACCCGGAATTCCG
                     TATGGCCATGAAAGATGGCGAGCTGGTAATTTGGGATTCCGTTCATCCGTGTTATACC
                     GTCTTTCACGAACAGACCGAGACGTTCTCTTCCCTGTGGTCTG"
     primer_bind     complement(6760..6779)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="10,588 R"
     misc_feature    7324..9196
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CGAGACGTTCTCTTCCCTGTGGTCTGAATATCACGATGACTT
                     CCGTCAATTCCTGCACATTTATTCTCAGGACGTTGCGTGCTATGGTGAGAATCTCGCT
                     TATTTTCCGAAGGGTTTCATTGAAAACATGTTCTTTGTTAGCGCCAACCCATGGGTGA
                     GCTTCACTTCTTTCGACCTGAACGTGGCGAACATGGACAACTTTTTCGCGCCGGTTTT
                     CACGATGGGCAAGTATTACACCCAGGGTGACAAGGTACTGATGCCGCTGGCAATCCAG
                     GTGCATCACGCGGTATGTGACGGCTTCCATGTCGGTCGCATGCTGAACGAGCTGCAGC
                     AATACTGTGATGAATGGCAGGGCGGTGCGTAAACGCGTGGATCCGGCTTACTAAAAGC
                     CAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGAT
                     ATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGAC
                     AGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTC
                     TGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGC
                     GGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTT
                     GCTGACGAGAACAGGGGCTGGTGAAATGCAATTTAAAGTTTATACCTACAAGCGCGAA
                     TCTCGCTACCGTCTGTTTGTAGACGTACAGTCTGATATCATTGATACGCCGGGCCGCC
                     GTATGGTTATTCCGCTGGCATCTGCACGTCTCCTGTCTGATAAAGTTAGCCGTGAACT
                     GTATCCGGTTGTACACATTGGCGACGAGTCTTGGCGCATGATGACGACTGACATGGCT
                     TCTGTCCCGGTTAGCGTCATTGGCGAGGAAGTCGCCGATCTGTCTCACCGTGAAAATG
                     ACATCAAAAATGCAATTAACCTGATGTTCTGGGGCATTTGATCCGGCTTACTAAAAGC
                     CAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGAT
                     ATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGAC
                     AGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTC
                     TGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGC
                     GGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTT
                     GCTGACGAGAACAGGGGCTGGTGAAATGCAATTTAAAGTTTATACCTACAAGCGCGAA
                     TCTCGCTACCGTCTGTTTGTAGACGTACAGTCTGATATCATTGATACGCCGGGCCGCC
                     GTATGGTTATTCCGCTGGCATCTGCACGTCTCCTGTCTGATAAAGTTAGCCGTGAACT
                     GTATCCGGTTGTACACATTGGCGACGAGTCTTGGCGCATGATGACGACTGACATGGCT
                     TCTGTCCCGGTTAGCGTCATTGGCGAGGAAGTCGCCGATCTGTCTCACCGTGAAAATG
                     ACATCAAAAATGCAATTAACCTGATGTTCTGGGGCATTTG"
     misc_feature    7326..7329
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      7330..7775
                     /note="Geneious type: promoter"
                     /note="Bacillus subtilis sacB (glycoside hydrolase family
                     68) promoter region"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB"
     gene            7776..9199
                     /note="counterselectable marker from Bacillus subtilis
                     that converts sucrose to levans"
                     /product="levansucrase"
                     /gene="sacB"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sacB CS"
     misc_feature    8167..8170
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    9197
                     /note="Geneious type: Junction"
                     /standard_name="junction 1"
     misc_feature    complement(9236..9359)
                     /note="Geneious type: attR6"
                     /standard_name="attR6"
     misc_feature    9360..9389
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    9360
                     /note="Geneious type: Junction"
                     /standard_name="junction 2"
     misc_feature    9362
                     /note="Geneious type: Junction"
                     /standard_name="junction 2"
     misc_feature    9390..9393
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     regulatory      complement(9452..9470)
                     /note="Geneious type: Promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7 prom"
     regulatory      complement(9453..9470)
                     /regulatory_class="promoter"
                     /note="T7 promoter"
                     /standard_name="T7 promoter"
     misc_feature    9483..9512
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
ORIGIN      
        1 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
       61 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
      121 tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
      181 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
      241 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
      301 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      361 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
      421 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
      481 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
      541 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
      601 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
      661 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
      721 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
      781 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
      841 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
      901 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
      961 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     1021 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1081 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     1141 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     1201 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     1261 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     1321 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca
     1381 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1441 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1501 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1561 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1621 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1681 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1741 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1801 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1861 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1921 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1981 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2041 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2101 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2161 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa atcgtcacct
     2221 gcaggaaggt ttaaacgcat ttaggtgaca ctatagaagt gtgtatcgct cgagggatcc
     2281 gaattcgaag tcttggtacg gaggggatcc gatcgacaag ccgaacatat gggttttttg
     2341 ctcacctgtg attgctccta ctcaaataca aaaacatcaa attttctgtc aataaagcat
     2401 atttatttat atttatttta caggaaagaa ttccttttaa agtgtatttt aacctataat
     2461 gaaaaacgat taaaaaaaat acataaaata attcgaaaat ttttgaatag cccaggttga
     2521 taaaaattca tttcatacgt tttataactt atgcccctaa gtattttttg accatagtgt
     2581 ttcaattcta cattaatttt acagagtaga atgaaacgcc acctactcag ccaagaggcg
     2641 aaaaggttag ctcgccaagc agagagggcg ccagtgctca ctacttttta taattctcaa
     2701 cttctttttc cagactcagt tcgtatatat agacctattt tcaatttaac gtcggggcct
     2761 caactttgta taataaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     2821 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     2881 tatgggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
     2941 ggattttgag ttaggatccg tcgagatttt caggagctac gcggaacccc tatttgttta
     3001 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3061 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3121 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3181 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3241 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3301 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3361 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3421 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3481 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3541 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3601 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3661 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3721 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3781 tctggagccg gtgagcgtgg gtcccgcggt atcattgcag cactggggcc agatggtaag
     3841 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3901 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaatggat ccggcttact
     3961 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     4021 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     4081 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     4141 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     4201 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     4261 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     4321 tatcgtctgt ttgtggatgt acagagtgat attatcgata caccgggccg tcgcatggtt
     4381 attccacttg cgtctgcgcg cttgctctca gataaagtct cccgtgaact ttacccggtg
     4441 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtt
     4501 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     4561 attaacctga tgttctgggg aatataatgt caggctccct tatacacagc cagtctgcac
     4621 gtcgcatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     4681 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa cttttctata
     4741 caaagttgtc cagctggagc gagaccggag caatggttaa ttcgagctcg cccggggatc
     4801 taattcaatt agagactaat tcaattagag ctaattcaat taggatccaa gcttatcgat
     4861 ttcgaaccct cgaccgccgg agtataaata gaggcgcttc gtctacggag cgacaattca
     4921 attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa acaagcgcag
     4981 ctgaacaagc taaacaatcg gatgaaaatt gaagagggca agttggtcat ctggattaat
     5041 ggagataagg gctataatgg actggcagag gttggaaaaa aatttgagaa ggatacaggc
     5101 attaaggtca cagttgagca tccagataag ttggaggaga agttcccgca ggtggctgcg
     5161 acgggtgacg gacccgacat catattctgg gctcatgacc gtttcggagg atacgcccag
     5221 tccggcttgt tggcggaaat tacccctgat aaagcttttc aggacaaact ctaccccttc
     5281 acctgggatg ctgtccgata caacggcaag ctcattgcat accccattgc agttgaagca
     5341 ctgagtctga tttacaataa agacctgttg ccgaatcctc cgaagacctg ggaggagatt
     5401 cccgctctgg acaaggagct gaaggctaag ggcaagtccg ccctgatgtt caacctgcaa
     5461 gagccgtact ttacttggcc cctgatcgca gccgacggcg gatacgcctt caaatacgag
     5521 aacggaaagt acgatataaa ggatgtcgga gtcgacaacg ccggcgctaa agcaggcctg
     5581 actttcctgg tggacctcat aaagaacaag catatgaatg ccgacaccga ttacagtata
     5641 gcggaagccg cgttcaacaa gggcgagact gccatgacaa taaacggacc ctgggcatgg
     5701 tccaacattg acacgagcaa ggtgaactac ggtgtaacgg tgttgcctac gttcaaggga
     5761 cagccgagta aaccattcgt tggcgttttg tcggctggaa ttaatgctgc tagtcccaat
     5821 aaggagttgg ctaaggagtt cttggagaac tacttgctga cagacgaggg cttggaggca
     5881 gtaaacaagg ataagcccct gggtgcggtt gccctcaagt cctatgaaga agagctcgta
     5941 aaagacccgc gtattgctgc gaccatggag aatgcccaga agggtgagat tatgcccaat
     6001 ataccccaga tgtccgcgtt ttggtatgca gttcgcaccg cggttataaa cgcagcctcg
     6061 ggacggcaga ccgtggatga ggacttgaaa gacgcccaga cacgcataac caaacagttt
     6121 cgcgaccata tggttctgca tgaatatgtc aacgcagcag gcattactta aagatcataa
     6181 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     6241 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     6301 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6361 attctagttg tggtttgtcc aaactcatca atgtatctta aagcttatcg atacgcccat
     6421 ggtctcacca tcaactttgt atacaaaagt tgaacgagaa acgtaaaatg atataaatat
     6481 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
     6541 tatccagtca ctatgggccg cattaggcac cccaggcttt acactttatg cttccggctc
     6601 gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa
     6661 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     6721 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     6781 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     6841 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
     6901 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     6961 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     7021 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     7081 gaatatgttt ttcgtgtcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     7141 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     7201 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     7261 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     7321 aacgcgtggc acatatacct gccgttcact attatttagt gaaatgagat attatgatat
     7381 tttctgaatt gtgattaaaa aggcaacttt atgcccatgc aacagaaact ataaaaaata
     7441 cagagaatga aaagaaacag atagattttt tagttcttta ggcccgtagt ctgcaaatcc
     7501 ttttatgatt ttctatcaaa caaaagagga aaatagacca gttgcaatcc aaacgagagt
     7561 ctaatagaat gaggtcgaaa agtaaatcgc gcgggtttgt tactgataaa gcaggcaaga
     7621 cctaaaatgt gtaaagggca aagtgtatac tttggcgtca ccccttacat attttaggtc
     7681 tttttttatt gtgcgtaact aacttgccat cttcaaacag gagggctgga agaagcagac
     7741 cgctaacaca gtacataaaa aaggagacat gaacgatgaa catcaaaaag tttgcaaaac
     7801 aagcaacagt attaaccttt actaccgcac tgctggcagg aggcgcaact caagcgtttg
     7861 cgaaagaaac gaaccaaaag ccatataagg aaacatacgg catttcccat attacacgcc
     7921 atgatatgct gcaaatccct gaacagcaaa aaaatgaaaa atatcaagtt cctgaattcg
     7981 attcgtccac aattaaaaat atctcttctg caaaaggcct ggacgtttgg gacagctggc
     8041 cattacaaaa cgctgacggc actgtcgcaa actatcacgg ctaccacatc gtctttgcat
     8101 tagccggaga tcctaaaaat gcggatgaca catcgattta catgttctat caaaaagtcg
     8161 gcgaaacttc tattgacagc tggaaaaacg ctggccgcgt ctttaaagac agcgacaaat
     8221 tcgatgcaaa tgattctatc ctaaaagacc aaacacaaga atggtcaggt tcagccacat
     8281 ttacatctga cggaaaaatc cgtttattct acactgattt ctccggtaaa cattacggca
     8341 aacaaacact gacaactgca caagttaacg tatcagcatc agacagctct ttgaacatca
     8401 acggtgtaga ggattataaa tcaatctttg acggtgacgg aaaaacgtat caaaatgtac
     8461 agcagttcat cgatgaaggc aactacagct caggcgacaa ccatacgctg agagatcctc
     8521 actacgtaga agataaaggc cacaaatact tagtatttga agcaaacact ggaactgaag
     8581 atggctacca aggcgaagaa tctttattta acaaagcata ctatggcaaa agcacatcat
     8641 tcttccgtca agaaagtcaa aaacttctgc aaagcgataa aaaacgcacg gctgagttag
     8701 caaacggcgc tctcggtatg attgagctaa acgatgatta cacactgaaa aaagtgatga
     8761 aaccgctgat tgcatctaac acagtaacag atgaaattga acgcgcgaac gtctttaaaa
     8821 tgaacggcaa atggtacctg ttcactgact cccgcggatc aaaaatgacg attgacggca
     8881 ttacgtctaa cgatatttac atgcttggtt atgtttctaa ttctttaact ggcccataca
     8941 agccgctgaa caaaactggc cttgtgttaa aaatggatct tgatcctaac gatgtaacct
     9001 ttacttactc acacttcgct gtacctcaag cgaaaggaaa caatgtcgtg attacaagct
     9061 atatgacaaa cagaggattc tacgcagaca aacaatcaac gtttgcgcca agcttcctgc
     9121 tgaacatcaa aggcaagaaa acatctgttg tcaaagacag catccttgaa caaggacaat
     9181 taacagttaa caaataaatg tcaggctccc ttatacacag ccagtctgca cgtcgcatag
     9241 tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt
     9301 taatatattg atatttatat cattttacgt ttctcgttca actttttaat acaaagttgc
     9361 ctccggaaca taatggtgca tctagatgcc cattcctgta gacttcttaa ttaagacgtc
     9421 agaattctcg aggcggccgc atgtgagtct ccctatagtg agtcgtatta atttcgcggg
     9481 cgcgtcagcg ggagagccgt tgtaaggcgg ca
//
