LOCUS       pAAV_CMV_PDGFRbH        7406 bp    DNA        circular     11-OCT-2022
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     repeat_region   1..130
                     /vntifkey="34"
                     /locus_tag="5'-ITR"
                     /label="5'-ITR"
                     /ApEinfo_label="5'-ITR"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    811..4128
                     /locus_tag="pdgfrb coding"
                     /label="pdgfrb coding"
                     /ApEinfo_label="pdgfrb coding"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     repeat_region   complement(4466..4595)
                     /vntifkey="34"
                     /locus_tag="3'-ITR"
                     /label="3'-ITR"
                     /ApEinfo_label="3'-ITR"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        211..793
                     /vntifkey="29"
                     /locus_tag="CMV\promotor"
                     /label="CMV\promotor"
                     /ApEinfo_label="CMV\promotor"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    4165..4378
                     /vntifkey="25"
                     /locus_tag="BGH\pA"
                     /label="BGH\pA"
                     /ApEinfo_label="BGH\pA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             5359..6219
                     /vntifkey="4"
                     /locus_tag="Amp-R"
                     /label="Amp-R"
                     /ApEinfo_label="Amp-R"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(4614..4631)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     7364..7384
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      6314..6996
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             4702..4770
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    7336..7358
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2808..2810
                     /locus_tag="N666K"
                     /label="N666K"
                     /ApEinfo_label="N666K"
                     /ApEinfo_fwdcolor="#ff2f92"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcgccc ttaagctagc tagttattaa tagtaatcaa ttacggggtc
      241 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      301 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      361 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      421 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      481 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      541 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      601 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      661 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      721 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
      781 tttagtgaac cgtcagatcc tgcaGACACC ATGCGGCTTC CGGGTGCGAT GCCAGCTCTG
      841 GCCCTCAAAG GCGAGCTGCT GTTGCTGTCT CTCCTGTTAC TTCTGGAACC ACAGATCTCT
      901 CAGGGCCTGG TCGTCACACC CCCGGGGCCA GAGCTTGTCC TCAATGTCTC CAGCACCTTC
      961 GTTCTGACCT GCTCGGGTTC AGCTCCGGTG GTGTGGGAAC GGATGTCCCA GGAGCCCCCA
     1021 CAGGAAATGG CCAAGGCCCA GGATGGCACC TTCTCCAGCG TGCTCACACT GACCAACCTC
     1081 ACTGGGCTAG ACACGGGAGA ATACTTTTGC ACCCACAATG ACTCCCGTGG ACTGGAGACC
     1141 GATGAGCGGA AACGGCTCTA CATCTTTGTG CCAGATCCCA CCGTGGGCTT CCTCCCTAAT
     1201 GATGCCGAGG AACTATTCAT CTTTCTCACG GAAATAACTG AGATCACCAT TCCATGCCGA
     1261 GTAACAGACC CACAGCTGGT GGTGACACTG CACGAGAAGA AAGGGGACGT TGCACTGCCT
     1321 GTCCCCTATG ATCACCAACG TGGCTTTTCT GGTATCTTTG AGGACAGAAG CTACATCTGC
     1381 AAAACCACCA TTGGGGACAG GGAGGTGGAT TCTGATGCCT ACTATGTCTA CAGACTCCAG
     1441 GTGTCATCCA TCAACGTCTC TGTGAACGCA GTGCAGACTG TGGTCCGCCA GGGTGAGAAC
     1501 ATCACCCTCA TGTGCATTGT GATCGGGAAT GAGGTGGTCA ACTTCGAGTG GACATACCCC
     1561 CGCAAAGAAA GTGGGCGGCT GGTGGAGCCG GTGACTGACT TCCTCTTGGA TATGCCTTAC
     1621 CACATCCGCT CCATCCTGCA CATCCCCAGT GCCGAGTTAG AAGACTCGGG GACCTACACC
     1681 TGCAATGTGA CGGAGAGTGT GAATGACCAT CAGGATGAAA AGGCCATCAA CATCACCGTG
     1741 GTTGAGAGCG GCTACGTGCG GCTCCTGGGA GAGGTGGGCA CACTACAATT TGCTGAGCTG
     1801 CATCGGAGCC GGACACTGCA GGTAGTGTTC GAGGCCTACC CACCGCCCAC TGTCCTGTGG
     1861 TTCAAAGACA ACCGCACCCT GGGCGACTCC AGCGCTGGCG AAATCGCCCT GTCCACGCGC
     1921 AACGTGTCGG AGACCCGGTA TGTGTCAGAG CTGACACTGG TTCGCGTGAA GGTGGCAGAG
     1981 GCTGGCCACT ACACCATGCG GGCCTTCCAT GAGGATGCTG AGGTCCAGCT CTCCTTCCAG
     2041 CTACAGATCA ATGTCCCTGT CCGAGTGCTG GAGCTAAGTG AGAGCCACCC TGACAGTGGG
     2101 GAACAGACAG TCCGCTGTCG TGGCCGGGGC ATGCCCCAGC CGAACATCAT CTGGTCTGCC
     2161 TGCAGAGACC TCAAAAGGTG TCCACGTGAG CTGCCGCCCA CGCTGCTGGG GAACAGTTCC
     2221 GAAGAGGAGA GCCAGCTGGA GACTAACGTG ACGTACTGGG AGGAGGAGCA GGAGTTTGAG
     2281 GTGGTGAGCA CACTGCGTCT GCAGCACGTG GATCGGCCAC TGTCGGTGCG CTGCACGCTG
     2341 CGCAACGCTG TGGGCCAGGA CACGCAGGAG GTCATCGTGG TGCCACACTC CTTGCCCTTT
     2401 AAGGTGGTGG TGATCTCAGC CATCCTGGCC CTGGTGGTGC TCACCATCAT CTCCCTTATC
     2461 ATCCTCATCA TGCTTTGGCA GAAGAAGCCA CGTTACGAGA TCCGATGGAA GGTGATTGAG
     2521 TCTGTGAGCT CTGACGGCCA TGAGTACATC TACGTGGACC CCATGCAGCT GCCCTATGAC
     2581 TCCACGTGGG AGCTGCCGCG GGACCAGCTT GTGCTGGGAC GCACCCTCGG CTCTGGGGCC
     2641 TTTGGGCAGG TGGTGGAGGC CACGGCTCAT GGCCTGAGCC ATTCTCAGGC CACGATGAAA
     2701 GTGGCCGTCA AGATGCTTAA ATCCACAGCC CGCAGCAGTG AGAAGCAAGC CCTTATGTCG
     2761 GAGCTGAAGA TCATGAGTCA CCTTGGGCCC CACCTGAACG TGGTCAAcct GTTGGGGGCC
     2821 TGCACCAAAG GAGGACCCAT CTATATCATC ACTGAGTACT GCCGCTACGG AGACCTGGTG
     2881 GACTACCTGC ACCGCAACAA ACACACCTTC CTGCAGCACC ACTCCGACAA GCGCCGCCCG
     2941 CCCAGCGCGG AGCTCTACAG CAATGCTCTG CCCGTTGGGC TCCCCCTGCC CAGCCATGTG
     3001 TCCTTGACCG GGGAGAGCGA CGGTGGCTAC ATGGACATGA GCAAGGACGA GTCGGTGGAC
     3061 TATGTGCCCA TGCTGGACAT GAAAGGAGAC GTCAAATATG CAGACATCGA GTCCTCCAAC
     3121 TACATGGCCC CTTACGATAA CTACGTTCCC TCTGCCCCTG AGAGGACCTG CCGAGCAACT
     3181 TTGATCAACG AGTCTCCAGT GCTAAGCTAC ATGGACCTCG TGGGCTTCAG CTACCAGGTG
     3241 GCCAATGGCA TGGAGTTTCT GGCCTCCAAG AACTGCGTCC ACAGAGACCT GGCGGCTAGG
     3301 AACGTGCTCA TCTGTGAAGG CAAGCTGGTC AAGATCTGTG ACTTTGGCCT GGCTCGAGAC
     3361 ATCATGCGGG ACTCGAATTA CATCTCCAAA GGCAGCACCT TTTTGCCTTT AAAGTGGATG
     3421 GCTCCGGAGA GCATCTTCAA CAGCCTCTAC ACCACCCTGA GCGACGTGTG GTCCTTCGGG
     3481 ATCCTGCTCT GGGAGATCTT CACCTTGGGT GGCACCCCTT ACCCAGAGCT GCCCATGAAC
     3541 GAGCAGTTCT ACAATGCCAT CAAACGGGGT TACCGCATGG CCCAGCCTGC CCATGCCTCC
     3601 GACGAGATCT ATGAGATCAT GCAGAAGTGC TGGGAAGAGA AGTTTGAGAT TCGGCCCCCC
     3661 TTCTCCCAGC TGGTGCTGCT TCTCGAGAGA CTGTTGGGCG AAGGTTACAA AAAGAAGTAC
     3721 CAGCAGGTGG ATGAGGAGTT TCTGAGGAGT GACCACCCAG CCATCCTTCG GTCCCAGGCC
     3781 CGCTTGCCTG GGTTCCATGG CCTCCGATCT CCCCTGGACA CCAGCTCCGT CCTCTATACT
     3841 GCCGTGCAGC CCAATGAGGG TGACAACGAC TATATCATCC CCCTGCCTGA CCCCAAACCC
     3901 GAGGTTGCTG ACGAGGGCCC ACTGGAGGGT TCCCCCAGCC TAGCCAGCTC CACCCTGAAT
     3961 GAAGTCAACA CCTCCTCAAC CATCTCCTGT GACAGCCCCC TGGAGCCCCA GGACGAACCA
     4021 GAGCCAGAGC CCCAGCTTGA GCTCCAGGTG GAGCCGGAGC CAGAGCTGGA ACAGTTGCCG
     4081 GATTCGGGGT GCCCTGCGCC TCGGGCGGAA GCAGAGGATA GCTTCCTGTA CCCTTACGAC
     4141 GTGCCAGACT ACGCCTAGag atctcctcga ctgtgccttc tagttgccag ccatctgttg
     4201 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     4261 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     4321 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggact
     4381 cgagttaagg gcgaattccc gattaggatc ttcctagagc atggctacgt agataagtag
     4441 catggcgggt taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct
     4501 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
     4561 gcccgggcgg cctcagtgag cgagcgagcg cgcagcctta attaacctaa ttcactggcc
     4621 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     4681 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     4741 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     4801 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4861 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4921 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4981 cttgattagg gtgatggttc acgtagtggg ccatcgcccc gatagacggt ttttcgccct
     5041 ttgacgctgg agttcacgtt cctcaatagt ggactcttgt tccaaactgg aacaacactc
     5101 aaccctatct cggtctattc ttttgattta taagggattt ttccgatttc ggcctattgg
     5161 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     5221 ataatttcag gtggcatctt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5281 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5341 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5401 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5461 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaatag tggtaagatc
     5521 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5581 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5641 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5701 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5761 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5821 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5881 gagcgtgaca ccacgatgcc tgtagtaatg gtaacaacgt tgcgcaaact attaactggc
     5941 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6001 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6061 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6121 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6181 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6241 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6301 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6361 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6421 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6481 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     6541 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6601 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6661 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6721 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6781 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6841 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6901 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6961 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7021 tgcggttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7081 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7141 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7201 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7261 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7321 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7381 catgattacg ccagatttaa ttaagg   
//
