LOCUS       Exported               11907 bp DNA     circular SYN 25-JAN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11907)
  AUTHORS   locusliu
  TITLE     Direct Submission
  JOURNAL   Exported Jun 18, 2023 from SnapGene Viewer 6.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(699..776)
                     /gene="lacI"
                     /label=lacI promoter
                     /note="color: #ffffff; direction: LEFT"
     promoter        1085..1103
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    1104..1128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     RBS             1143..1165
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
                     /note="color: #a6acb3"
     CDS             1173..1205
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /note="color: #cc99b2"
                     /translation="MASMTGGQQMG"
     CDS             1215..1241
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /note="color: #993366"
                     /translation="PAAKKKKLD"
     CDS             1287..1307
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             1308..5408
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /note="color: #993366"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(4071..4121)
                     /label=D10-PE-F2-3p
                     /note="color: black; sequence: 
                     catccgggagtccaggatctgtgccacgtgctttgtgatctgccgggtttc; added:
                     2023-01-25"
     CDS             5451..5477
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             5499..5519
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     misc_feature    5544..7586
                     /label=M-MLV
                     /note="color: #0000ff"
     primer_bind     6136..6159
                     /label=RT-233-435-seq
                     /note="color: black; sequence: tgtttaacgaggccctgcaccgcg; 
                     added: 2023-01-24"
     CDS             6210..6212
                     /codon_start=1
                     /label=V233M
                     /note="color: #993366"
                     /translation="V"
     misc_feature    6846..6848
                     /label=L435k
                     /note="color: #ff0000"
     CDS             7632..7652
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             7665..7712
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7719..7736
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     terminator      7803..7850
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      7887..8342
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     8003..8021
                     /label=D10-PE-F2-3p
                     /note="color: black; sequence: 
                     catccgggagtccaggatctgtgccacgtgctttgtgatctgccgggtttc; added:
                     2023-01-25"
     promoter        8369..8473
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             8474..9334
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8474 .. 8542 / #ccffcc / signal sequence
                        2: 8543 .. 9334 / #ccffcc
                     Cleavage site after base 8542"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9505..10093
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     misc_feature    10279..10421
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     CDS             complement(10523..10714)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /note="color: #993366"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(11523..11907,1..698))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #993366"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
       61 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
      121 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
      181 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
      241 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
      301 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
      361 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
      421 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
      481 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
      541 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
      601 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
      661 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
      721 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
      781 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
      841 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
      901 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
      961 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     1021 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     1081 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
     1141 attttgttta actttaagaa ggagatatac atatggctag catgactggt ggacagcaaa
     1201 tgggtcgcgc cgctccggca gctaagaaaa agaaactgga tggcagcgtc gacaaacgga
     1261 cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtcgac aagaagtaca
     1321 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
     1381 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     1441 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
     1501 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     1561 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     1621 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     1681 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     1741 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     1801 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     1861 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     1921 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagaaagctg gaaaatctga
     1981 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     2041 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     2101 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     2161 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     2221 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     2281 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     2341 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     2401 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     2461 ccgaggaact gctcgtgaag ctgaagagag aggacctgct gcggaagcag cggaccttcg
     2521 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     2581 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     2641 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     2701 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     2761 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     2821 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     2881 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     2941 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     3001 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     3061 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     3121 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     3181 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     3241 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     3301 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     3361 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     3421 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     3481 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     3541 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     3601 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     3661 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     3721 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     3781 aggaactgga catcaaccgg ctgtccgact acgatgtgga cgctatcgtg cctcagagct
     3841 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     3901 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     3961 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     4021 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     4081 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     4141 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     4201 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     4261 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     4321 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     4381 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     4441 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     4501 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     4561 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     4621 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     4681 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     4741 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     4801 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     4861 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     4921 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     4981 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     5041 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     5101 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     5161 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     5221 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     5281 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     5341 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     5401 gaggtgactc cggcggaagc tctggtggca gcaagcggac cgccggctct tacccatacg
     5461 atgttccaga ttacgctgac ggctctgaat tcgagagccc taagaagaaa agaaaggtga
     5521 gcggaggctc tagcggcgga agcaccctga acattgaaga cgagtataga ctgcatgaaa
     5581 caagcaagga acccgacgtg tccctgggct ccacctggct gtccgacttt ccccaggcct
     5641 gggccgagac aggaggaatg ggcctggccg tgcggcaggc acccctgatc atccctctga
     5701 aggccacctc tacacccgtg agcatcaagc agtaccctat gtctcaggag gccagactgg
     5761 gcatcaagcc tcacatccag aggctgctgg accagggcat cctggtgcca tgccagagcc
     5821 cctggaacac accactgctg cccgtgaaga agccaggcac caatgactat agacccgtgc
     5881 aggatctgag agaggtgaac aagagggtgg aggatatcca ccccaccgtg cccaaccctt
     5941 acaatctgct gtccggcctg cccccttctc accagtggta tacagtgctg gacctgaagg
     6001 atgccttctt ttgtctgaga ctgcacccta ccagccagcc actgttcgcc tttgagtgga
     6061 gggaccctga gatgggcatc tctggccagc tgacctggac acgcctgcct cagggcttca
     6121 agaatagccc aacactgttt aacgaggccc tgcaccgcga cctggcagat ttccggatcc
     6181 agcacccaga tctgatcctg ctgcagtacg tggacgatct gctgctggcc gccaccagcg
     6241 agctggattg ccagcaggga acacgcgccc tgctgcagac cctgggaaac ctgggatata
     6301 gggcatccgc caagaaggcc cagatctgtc agaagcaggt gaagtacctg ggctatctgc
     6361 tgaaggaggg ccagagatgg ctgacagagg ccaggaagga gacagtgatg ggccagccaa
     6421 cacccaagac cccaagacag ctgagggagt tcctgggcaa agcaggattt tgcaggctgt
     6481 tcatcccagg attcgcagag atggcagcac ctctgtaccc actgaccaag ccgggcaccc
     6541 tgtttaattg gggccctgac cagcagaagg cctatcagga gatcaagcag gccctgctga
     6601 cagcaccagc cctgggcctg ccagacctga ccaagccttt cgagctgttt gtggatgaga
     6661 agcagggcta cgccaagggc gtgctgaccc agaagctggg accatggaga cggcccgtgg
     6721 cctatctgtc caagaagctg gacccagtgg cagcaggatg gccaccatgc ctgaggatgg
     6781 tggcagcaat cgccgtgctg acaaaggatg ccggcaagct gaccatggga cagccactgg
     6841 tcatcctggc accacacgca gtggaggccc tggtgaagca gcctccagat cgctggctgt
     6901 ctaacgcccg gatgacacac taccaggccc tgctgctgga caccgatcgc gtgcagtttg
     6961 gccctgtggt ggccctgaat ccagccaccc tgctgcctct gccagaggag ggcctgcagc
     7021 acaactgtct ggacatcctg gcagaggcac acggaacaag gccagacctg accgatcagc
     7081 ccctgcctga cgccgatcac acatggtata ccgatggaag ctccctgctg caggagggcc
     7141 agaggaaggc aggagcagca gtgaccacag agacagaagt gatctgggcc aaggccctgc
     7201 cagcaggcac atccgcccag cgggccgagc tgatcgccct gacccaggcc ctgaagatgg
     7261 ccgagggcaa gaagctgaac gtgtacacag actccagata tgccttcgcc accgcacaca
     7321 tccacggaga gatctacagg cgccggggct ggctgacctc tgagggcaag gagatcaaga
     7381 acaaggatga gatcctggcc ctgctgaagg ccctgtttct gcccaagcgg ctgagcatca
     7441 tccactgtcc tggacaccag aagggacact ccgccgaggc aaggggcaat cggatggccg
     7501 accaggccgc cagaaaggct gctattactg aaactcccga cacttccact ctgctgattg
     7561 aaaactcctc cccttctggc ggctcaaccg gtggtggtcc cgggggtggt gcggccgcag
     7621 gcagcggaag ccctaagaaa aaacgaaaag ttggcagcgg aagcaaaagg ccggcggcca
     7681 cgaaaaaggc cggccaggca aaaaagaaaa agctcgagca ccaccaccac caccactgag
     7741 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     7801 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
     7861 gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     7921 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7981 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8041 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8101 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8161 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8221 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     8281 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     8341 ttcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     8401 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     8461 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     8521 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     8581 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     8641 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     8701 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     8761 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     8821 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     8881 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     8941 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     9001 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     9061 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     9121 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     9181 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     9241 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     9301 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     9361 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     9421 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9481 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     9541 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     9601 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     9661 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9721 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     9781 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     9841 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     9901 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     9961 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    10021 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    10081 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    10141 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    10201 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    10261 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
    10321 acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
    10381 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
    10441 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
    10501 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
    10561 agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
    10621 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg
    10681 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc
    10741 tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg
    10801 atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc
    10861 ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag
    10921 gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg
    10981 gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg
    11041 ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg
    11101 attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga
    11161 gcacgatcat gcgcacccgt ggggccgcca tgccggcgat aatggcctgc ttctcgccga
    11221 aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata
    11281 ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga
    11341 cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg
    11401 cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca
    11461 agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc
    11521 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    11581 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
    11641 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
    11701 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
    11761 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc
    11821 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
    11881 atcgcagtgg gaacgatgcc ctcattc
//
