LOCUS       pJT87_v2                9571 bp    DNA     circular UNK 19-DEC-2023
DEFINITION  .
ACCESSION   <unknown
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="ori"
     CDS             771..864
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="8.679593721144968"
                     /fragment="True"
                     /other="CDS"
                     /label="lacI"
     protein_bind    complement(877..898)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
                     /label="CAP binding site"
     promoter        913..943
                     /note="pLannotate"
                     /database="addgene"
                     /identity="96.875"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="lac promoter"
     protein_bind    complement(947..969)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="95.83333333333334"
                     /fragment="False"
                     /other="protein bind"
                     /label="lac operator"
     promoter        1012..1030
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="T3 promoter"
     misc_feature    complement(1060..1896)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
                     /label="HA-R"
     CDS             2017..2056
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="3.6934441366574333"
                     /fragment="True"
                     /other="CDS"
                     /label="lacI"
     protein_bind    complement(2069..2090)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
                     /label="CAP binding site"
     promoter        2105..2135
                     /note="pLannotate"
                     /database="addgene"
                     /identity="96.875"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="lac promoter"
     protein_bind    complement(2139..2161)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="95.83333333333334"
                     /fragment="False"
                     /other="protein bind"
                     /label="lac operator"
     polyA_site      complement(2544..2599)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA site"
                     /label="-beta-globin poly(A) signal"
     protein_bind    2754..2778
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
                     /label="attB2"
     protein_bind    complement(4238..4260)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="92.0"
                     /fragment="True"
                     /other="protein bind"
                     /label="attB1"
     intron          complement(4318..5331)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="99.704"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
                     /label="chimeric intron"
     promoter        complement(5338..5617)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="98.233"
                     /match_length="100.71174377224199"
                     /fragment="False"
                     /other="promoter"
                     /label="chicken beta-actin promoter"
     enhancer        complement(5619..5985)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="96.83377308707124"
                     /fragment="False"
                     /other="enhancer"
                     /label="CMV enhancer"
     promoter        complement(6032..6050)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="T7 promoter"
     CDS             6053..6129
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="2.5040650406504064"
                     /fragment="True"
                     /other="CDS"
                     /label="lacZ"
     protein_bind    complement(6155..6175)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
                     /label="attB4"
     misc_feature    complement(6847..6872)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
                     /label="SA"
     misc_feature    complement(6951..7754)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
                     /label="HA-L"
     promoter        complement(7769..7787)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="T7 promoter"
     CDS             7795..7953
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="5.170731707317073"
                     /fragment="True"
                     /other="CDS"
                     /label="lacZ"
     rep_origin      7955..8410
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="f1 ori"
     promoter        8436..8540
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="AmpR promoter"
     CDS             8541..9401
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="AmpR"
ORIGIN      
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcca ccgcggtgga gagcagagcc aggaacccct
     1081 gtagggaagg ggcaggagag ccaggggcat gagatggtgg acgaggaagg gggacaggga
     1141 agcctgagcg cctctcctgg gcttgccaag gactcaaacc cagaagccca gagcagggcc
     1201 ttagggaagc gggaccctgc tctgggcgga ggaatatgtc ccagatagca ctggggactc
     1261 tttaaggaaa gaaggatgga gaaagagaaa gggagtagag gcggccacga cctggtgaac
     1321 acctaggacg caccattctc acaaagggag ttttccacac ggacaccccc ctcctcacca
     1381 cagccctgcc aggacggggc tggctactgg ccttatctca caggtaaaac tgacgcacgg
     1441 aggaacaata taaattgggg actagaaagg tgaagagcca aagttagaac tcaggaccaa
     1501 cttattctga ttttgttttt ccaaactgct tctcctcttg ggaagtgtaa ggaagctgca
     1561 gcaccaggat cagtgaaacg caccagacag ccgcgtcaga gcagctcagg ttctgggaga
     1621 gggtagcgca gggtggccac tgagaaccgg gcaggtcacg catccccccc ttccctccca
     1681 ccccctgcca agctctccct cccaggatcc tctctggctc catcgtaagc aaaccttaga
     1741 ggttctggca aggagagaga tggctccagg aaatgggggt gtgtcaccag ataaggaatc
     1801 tgcctaacag gaggtggggg ttagacccaa tatcaggaga ctaggaagga ggaggcctaa
     1861 ggatggggct tttctgtcac caatcctgtc cctagtagcg gccgcgtcga cgtttaaacc
     1921 tcgagattac cctgttatcc ctattaatta aggatcttaa aaacattata caatactaca
     1981 agcataaaaa cgcaccaagc ttagtactgt cgatccgctg gcacgacagg tttcccgact
     2041 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     2101 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     2161 ttcacacagg aaacagctat gaccatgatt acgccaagct tgggctgcag gtcgagggat
     2221 ctccataaga gaagagggac agctatgact gggagtagtc aggagaggag gaaaaatctg
     2281 gctagtaaaa catgtaagga aaattttagg gatgttaaag aaaaaaataa cacaaaacaa
     2341 aatataaaaa aaatctaacc tcaagtcaag gcttttctat ggaataagga atggacagca
     2401 gggggctgtt tcatatactg atgacctctt tatagccaac ctttgttcat ggcagccagc
     2461 atatgggcat atgttgccaa actctaaacc aaatactcat tctgatgttt taaatgattt
     2521 gccctcccat atgtccttcc gagtgagaga cacaaaaaat tccaacacac tattgcaatg
     2581 aaaataaatt tcctttatta gccagaagtc agatgctcaa ggggcttcat gatgtcccca
     2641 taatttttgg cagagggaaa aagatctcag tggtatttgt gagccagggc attggccaca
     2701 ccagccacca ccttctgata ggcagcctgc acctgaggag tgaattcacg cgtaccactt
     2761 tgtacaagaa agctgggtat cacaggctga aggtgtattc gatgtcaatg tatttgtctt
     2821 tgctcagggt aattttcttg atcagagtct tgaacacttc atgcatatct ttttcctcgt
     2881 ccagcattga aaaagcctcc ttaattttct tttcctcctt gtttcgcttg ttctggtctg
     2941 tcagcttcag cagggccagt tcctgctctt tgatttcatt ctcgaagttc aggtcgcgct
     3001 ttgaaaacat ttctttatca atcaggccct cgacgtacag gtcgatcagc ttttctttct
     3061 tcactttcag cagcttaatc tgtttcttga tctcggtact cttgtcattc cgtttctgat
     3121 gcatctttgg gttaaagtgg atttccagct ctttttcctg ctgcttcagt ccgtagatga
     3181 tggcctcgcg aatatcgtcg tagtgtgcag gatcgaaatt cacgcagcgg ttaaacctga
     3241 tataattgga gcacttcatg tacacccact ctttcttgga ccccttagca tacctgccgc
     3301 tgtatttggc agtgataggt tctccacaat gcttgcagaa catcatcccc cgaaactcgt
     3361 ttcgagtgtc ctgttttgtt ttcttttttg atgcgcgatc actattgaca gcgttccaca
     3421 gctccttact cacgattgta ggatagtggt cttcaatgat tgtcagcctc tccttggggg
     3481 tgtacactct ctttttcttg ccatcgactt tcacagtctt ataactattc aggaccaggt
     3541 tccccttgta tgtctgattt ttcaggatct tgcccactgt ggtatgagac cacagctggc
     3601 cgaacttggt ataagttcct ttgtcattca gaatgtttgc gattttcttg aacccatagc
     3661 ccttgtagct cagatcaaaa atctctctca cgatctcagc ttctttctcg ttaatgacca
     3721 gtttcttatc gatcacgtcg tatcccagcc cgggctttcc gataaactcc ccgcgccttg
     3781 ctttagcctg catggctgca gagacggaca cgctaatggt ctttggcagc tgagcggcga
     3841 acatggcaaa catttcgaat ttgatatcat tcccgccctc atacagagag tcgtagtttt
     3901 cctcaatagt caccagccgg atgccatgtc caatcaggat ttccttaatc tccagggcat
     3961 ctctcaggtc ccgtgccagt cggtggatag acttgaagac cactgtatcc agttcatttc
     4021 tccgggcttt ctccagaatc agctgcatag cgtgcctctc cagccatgcg gttccggaga
     4081 ttccatcgtc aaagtacacg gcattggggt cccattcata cccgttcttc tccagccagt
     4141 atctgcaaat atcgatctgg ttttcgacgg aagagacctg ctcatcccta tctgtgctga
     4201 ctctgacgta gactgcgtat ttcatggtgg caggcctcct gcttttttgt acaaacttgg
     4261 gtcgaattct ttgccaaaat gatgagacag cacaataacc agcacgttgc ccaggagctg
     4321 taggaaaaag aagaaggcat gaacatggtt agcagaggct ctagagccgc cggtcacacg
     4381 ccagaagccg aaccccgccc tgccccgtcc cccccgaagg cagccgtccc cccgcggaca
     4441 gccccgaggc tggagaggga gaaggggacg gcggcgcggc gacgcacgaa ggccctcccc
     4501 gcccatttcc ttcctgccgg cgccgcaccg cttcgccccg cgcccgctag agggggtgcg
     4561 gcggcgcctc ccagatttcg gctccgcaca gatttgggac aaaggaagtc cctgcgccct
     4621 ctcgcacgat taccataaaa ggcaatggct gcggctcgcc gcgcctcgac agccgccggc
     4681 gctccggggg ccgccgcgcc cctcccccga gccctccccg gcccgaggcg gccccgcccc
     4741 gcccggcacc cccacctgcc gccacccccc gcccggcacg gcgagccccg cgccacgccc
     4801 cgtacggagc cccgcacccg aagccgggcc gtgctcagca actcggggag gggggtgcag
     4861 ggggggttgc agcccgaccg acgcgcccac accccctgct caccccccca cgcacacacc
     4921 ccgcccgcag cctttgttcc cctcgcagcc ccccccgcac cgcggggcac cgcccccggc
     4981 cgcgctcccc tcgcgcacac tgcggagcgc acaaagcccc gcgccgcgcc cgcagcgctc
     5041 acagccgccg ggcagcgcgg agccgcacgc ggcgctcccc acgcacacac acacgcacgc
     5101 accccccgag ccgctccccc cgcacaaagg gccctcccgg agcccctcaa ggctttcacg
     5161 cagccacaga aaagaaacaa gccgtcatta aaccaagcgc taattacagc ccggaggaga
     5221 agggccgtcc cgcccgctca cctgtgggag taacgcggtc agtcagagcc ggggcgggcg
     5281 gcgcgaggcg gcggcggagc ggggcacggg gcgaaggcaa cgcagcgact cgactcccgc
     5341 ccgccgcgcg cttcgctttt tatagggccg ccgccgccgc cgcctcgcca taaaaggaaa
     5401 ctttcggagc gcgccgctct gattggctgc cgccgcacct ctccgcctcg ccccgccccg
     5461 cccctcgccc cgccccgccc cgcctggcgc gcgccccccc cccccccccg cccccatcgc
     5521 tgcacaaaat aattaaaaaa taaataaata caaaattggg ggtggggagg ggggggggag
     5581 atggggagag tgaagcagaa cgtggggctc acctcgacca tggtaatagc gatgactaat
     5641 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     5701 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     5761 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     5821 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     5881 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     5941 ctatgaacta atgaccccgt aattgattac tattaataac tagtccaccg cggtggagct
     6001 cgcgcgctac gtaggcctag ctccaattcg ccctatagtg agtcgtatta caattcactg
     6061 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     6121 gcagcacatt taattaactc gagaaattcg gagccaactt ttctatacaa agttgatatc
     6181 actagcgtta acgtcgacat cgatacggta ccgagttggc gcgcctggga gctcacgggg
     6241 acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc
     6301 agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag
     6361 cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt
     6421 ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaagc tttaggctga
     6481 aagagagatt tagaatgaca ggcgcgcctg ggagctcacg gggacagccc cctcccaaag
     6541 cccccaggga tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc
     6601 tccgctccgg tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg
     6661 ggcacgggga aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc
     6721 ctgcagacac ctggggggat acggggaaaa agctttaggc tgaaagagag atttagaatg
     6781 acaggcgcgc caactcgaat tcattgcaga ctttcactgg taccatcgat gcaattgtct
     6841 cgaggccctg tgggaggaag agaagaggtc agaagcttgg cacagtcaat gaagtttgaa
     6901 aaccaataag agctcggtaa accggttagg gataacaggg taataagctt gccccactgt
     6961 ggggtggagg ggacagataa aagtacccag aaccagagcc acattaaccg gccctgggaa
     7021 tataaggtgg tcccagctcg gggacacagg atccctggag gcagcaaaca tgctgtcctg
     7081 aagtggacat aggggcccgg gttggaggaa gaagactagc tgagctctcg gacccctgga
     7141 agatgccatg acagggggct ggaagagcta gcacagacta gagaggtaag gggggtaggg
     7201 gagctgccca aatgaaagga gtgagaggtg acccgaatcc acaggagaac ggggtgtcca
     7261 ggcaaagaaa gcaagaggat ggagaggtgg ctaaagccag ggagacgggg tactttgggg
     7321 ttgtccagaa aaacggtgat gatgcaggcc tacaagaagg ggaggcggga cgcaagggag
     7381 acatccgtcg gagaaggcca tcctaagaaa cgagagatgg cacaggcccc agaaggagaa
     7441 ggaaaaggga acccagcgag tgaagacggc atggggttgg gtgagggagg agagatgccc
     7501 ggagaggacc cagacacggg gaggatccgc tcagaggaca tcacgtggtg cagcgccgag
     7561 aaggaagtgc tccggaaaga gcatccttgg gcagcaacac agcagagagc aaggggaaga
     7621 gggagtggag gaagacggaa cctgaaggag gcggcaggga aggatctggg ccagccgtag
     7681 aggtgaccca ggccacaagc tgcagacaga aagcggcaca ggcccagggg agagaatgct
     7741 ggtcagagaa agcaggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     7801 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     7861 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     7921 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     7981 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     8041 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     8101 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     8161 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     8221 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     8281 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     8341 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     8401 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     8461 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     8521 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     8581 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     8641 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     8701 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8761 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     8821 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8881 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8941 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     9001 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     9061 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     9121 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     9181 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     9241 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     9301 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     9361 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     9421 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     9481 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     9541 cagaccccgt agaaaagatc aaaggatctt c
//
