LOCUS       pHIT182        9513 bp ds-DNA     circular SYN 18-JUN-2023
DEFINITION  Bacterial expression of catalytically defective CSR-1a with 6xHis 
            and maltose binding protein tags for in vitro assays
ACCESSION   .
VERSION     .
KEYWORDS    pHIT182
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1
  AUTHORS   Aoki K, Moriguchi H, Yoshioka T, Okawa K, Tabara H
  TITLE     In vitro analyses of the production and activity of secondary small
            interfering RNAs in C. elegans
  JOURNAL   EMBO J. 2007 Dec 12;26(24):5007-19. doi: 10.1038/sj.emboj.7601910.
   PUBMED   18007599
COMMENT     
ORIGIN
        1 taatacgact cactatagGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT
       61 TGTTTAACTT TAAGAAGGAG ATATACCatg GGCAGCAGCC ATCATCATCA TCATCACAGC
      121 AGCGGCCTGG TGCCGCGCGG CAGCCATAAT atgaaaactg aagaaggtaa actggtaatc
      181 tggattaacg gcgataaagg ctataacggt ctcgctgaag tcggtaagaa attcgagaaa
      241 gataccggaa ttaaagtcac cgttgagcat ccggataaac tggaagagaa attcccacag
      301 gttgcggcaa ctggcgatgg ccctgacatt atcttctggg cacacgaccg ctttggtggc
      361 tacgctcaat ctggcctgtt ggctgaaatc accccggaca aagcgttcca ggacaagctg
      421 tatccgttta cctgggatgc cgtacgttac aacggcaagc tgattgctta cccgatcgct
      481 gttgaagcgt tatcgctgat ttataacaaa gatctgctgc cgaacccgcc aaaaacctgg
      541 gaagagatcc cggcgctgga taaagaactg aaagcgaaag gtaagagcgc gctgatgttc
      601 aacctgcaag aaccgtactt cacctggccg ctgattgctg ctgacggggg ttatgcgttc
      661 aagtatgaaa acggcaagta cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa
      721 gcgggtctga ccttcctggt tgacctgatt aaaaacaaac acatgaatgc agacaccgat
      781 tactccatcg cagaagctgc ctttaataaa ggcgaaacag cgatgaccat caacggcccg
      841 tgggcatggt ccaacatcga caccagcaaa gtgaattatg gtgtaacggt actgccgacc
      901 ttcaagggtc aaccatccaa accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc
      961 agtccgaaca aagagctggc aaaagagttc ctcgaaaact atctgctgac tgatgaaggt
     1021 ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag cgctgaagtc ttacgaggaa
     1081 gagttggcga aagatccacg tattgccgcc actatggaaa acgcccagaa aggtgaaatc
     1141 atgccgaaca tcccgcagat gtccgctttc tggtatgccg tgcgtactgc ggtgatcaac
     1201 gccgccagcg gtcgtcagac tgtcgatgaa gccctgaaag acgcgcagac tCATATGGCG
     1261 GCCGCGcagt cggataatgt cggtcgtggc cgtggaagcc gtggtggttc tcgtggaggt
     1321 agtggacgtg gtagaggacg tggaggatac ggagactcgt atcgtccgga tttagaaacg
     1381 agagaaaccc atgtaacgta tcaaggcaag gtaaagtatg aaggaggaga ccgtcatgat
     1441 ttcaactatg agaagacgcc aactggaagt tatcgtggct cgcatcgaga tggtcaggaa
     1501 ggatcgagag gcaaccgcgg atcattcaga ggtggacgag gaggttacga cagaagacgt
     1561 gatgatgaag aatcaaaagc agaaaatcgt tttggagggt acaatcgggg agcaaatggc
     1621 ggaaactatc gcgggagaac tggcgggagc ggcagaggtg gatcgaccca aaccactacc
     1681 gtggatccta aatataatgg attgatcttg gaaaattcag cagagccaaa gcttaaagga
     1741 cttaactctg acatgaatca gaagcaaaat ccaagacttg ccctgaacat cttcgggctt
     1801 gagctctctg agcgaacgat tttccggcat gttgtgcaaa tgaagctcat cgatcgtcag
     1861 cacaataagg agtatattct tacaacaatg agtgccagag gacgtggaaa ccgtgcaaca
     1921 aagcaaaaag ataactttat tcttcttgac atcctactca aacagtgggc cgcgaagaag
     1981 ggtcagcaga atcttcctgc attcgcgtac gacggtgcac agtctttatt cactttggag
     2041 ggaatcagct tgatggtcga tatcaagaaa gaagatgctc tcgaaatccc ggaactttcc
     2101 aattttctga aggattccat ctcgttcctc agcggcgatt tggagatctc ttgtgaaccg
     2161 gatctcgaga aaccgtcatt cgttcagacg gagcttaacg aatggagtga tccaagattc
     2221 tacgcttatt tggatattgt gacatcacag agtgccatta gaagcgaaag atatttgtcg
     2281 cagtcgaaag gtctctatgt ccacactcaa agcttggagg agcttcgcgt gaaatgggct
     2341 gttgcagcaa aaggaatcca caaaggatgc cgaatcgtcg gagcaaacgg acctcttcca
     2401 attctagaac tcgatcctca atcgactcag tactatgctt caatcccact cagtcagatg
     2461 ttgcagtacg catttcctcg tgattttccg ccaaaccgta tcgttaatcc aaatatgaaa
     2521 ctgcaaagag ccgtgaaact attgttgaag gatttaaaat gcaacccata ttacgatgac
     2581 aaacagattt gggctacgaa cactatcaca gtgtcggatg ttgactacaa cgcgccgaaa
     2641 gatccagaat tcagacaaaa gtatccaaat ctgaaattcc caatgcttcc tgcagttcaa
     2701 tgcggcactg gaccacacaa gcgcctcatg ccgctcgaat atttgaaggt tcttccttat
     2761 caatccattg accgccgtgt actcgaagag tttgagctta caccacgagc gaatgccccg
     2821 aacgaacgct ggtcaactct tcaaaaacac tacgatcaat ttggtttcaa cgatcaagtc
     2881 atgaaagatt ttggtgtcca aatctgcaat gatcctttca acaacgtcag tgagatcgac
     2941 ggtgaaagag tcctcgcgcc tagtgttgca tatgcagacc cggtacacgt ggatgatgag
     3001 aagcgtgact ggaaagcaca agacaaaaag tttgttacac ctgctacgat tgaccatttg
     3061 atgttcgttc tagtagcagg ttatactcga acttgggatg ctgattgcga tgcaaccaaa
     3121 tttgtcgcga aagcattcat gcagcgttgc aaggacaaag gaatgcacat tggcagttat
     3181 agcatggatc agcacaatgg cgaacgaggc agcgaaaatt tccttacttc ggtcttcaaa
     3241 aacttggtga ctcatccaaa ctaccgggat tcgtctttca ctccgttcgt cttgtttatt
     3301 tccgatgacg ttcctaacat tcatgagtgc ctcaaattcg aagagcgtat gagtgacatt
     3361 ccaacgcagc acgtacttct caaaaatgtc aaaaagatgc gtgacaacat cgaaaagaag
     3421 tctcaaggtg gaagaagagc atatgatttg actcttgaca atattgtgat gaaagccaac
     3481 attaaatgcg gtggacttaa ctacacagca gatattccaa gagatcttgc gtgctggaat
     3541 gaagtttcta ccttcgtaat tggaatggCc gttgctcatc ctgaccgaaa cgctgcgaga
     3601 gaaggaaatc catccaccgt cggactgtcc tgcaattctg ctgaaaaccc atactcgttc
     3661 attggagact tcttatacac cgatcctaga agagaggcga tccaggatga aattttgcga
     3721 aaattcactg accaaagcgt tcgaaacttt gccgaaattc gaggtttccc aaagaaagta
     3781 atcatcttcc gtgatggtgt ttcattcggt gaagagactg cggctctgaa ggaggtggaa
     3841 atcattgagc aaaccattaa gacagccgct aagtccatgg gccattctga ttatgctcca
     3901 aaagtattag ccattgttgt taaaaaacgt caccacactc gattctacgc caagggagga
     3961 catcacggga acatgccgat caatccgctt cccgatacat ctgttggtgg tgatattgcc
     4021 gaatatggaa aaagacaaat cttcatccaa gctttccggc cagtgcaagg tactgcaaag
     4081 gttccatcat tcttggttat tcgagatgat gaggaagttt cggacgaaca tgttgccaaa
     4141 atggtttgcg ccgtatgcag tcttcatcag cttgttaaca gtccaacatc catcccaact
     4201 ccagtttacg ttgctcatga gctcgccaag agaggaaccg ggctttacaa agcttatagg
     4261 ttcaagaatg gcgaactctt tgatgattgg gaaacgctca ccactcaact ctcctatagc
     4321 actctcgatc gactttcaaa agttcgagtt gtttagTCGA CAGGCCTGTC GAGCACCACC
     4381 ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG
     4441 CCACCGCTGA GCAATAActa gcataacccc ttggggcctc taaacgggtc ttgaggggtt
     4501 ttttgCTGAA AGGAGGAACT ATATCCGGAT TGGCGAATGG GACGCGCCCT GTAGCGGCGC
     4561 ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT
     4621 AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG
     4681 TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA
     4741 CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT
     4801 TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG
     4861 AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC
     4921 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT
     4981 ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG
     5041 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT
     5101 CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT
     5161 GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA
     5221 GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC
     5281 CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG
     5341 AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT
     5401 CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA
     5461 GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC
     5521 GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA
     5581 CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC
     5641 GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA
     5701 TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG
     5761 CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG
     5821 CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG
     5881 ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA
     5941 GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC
     6001 CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC
     6061 TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA
     6121 ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA
     6181 GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT
     6241 CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG
     6301 GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC
     6361 ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA
     6421 TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG
     6481 GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT
     6541 CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG
     6601 CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG
     6661 CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC
     6721 GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG
     6781 AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT
     6841 TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC
     6901 AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC
     6961 ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT
     7021 GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG
     7081 GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC
     7141 ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG
     7201 GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT
     7261 TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC
     7321 TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT
     7381 GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT
     7441 AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA
     7501 GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT
     7561 TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG
     7621 TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG
     7681 GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC
     7741 GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA
     7801 TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT
     7861 GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG
     7921 TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT
     7981 CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT
     8041 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG
     8101 CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA
     8161 GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC
     8221 GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA
     8281 TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC
     8341 GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA
     8401 GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA
     8461 TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT
     8521 TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG
     8581 CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT
     8641 GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA
     8701 CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA
     8761 TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC
     8821 CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA
     8881 TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA
     8941 GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG
     9001 CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA
     9061 CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG
     9121 GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA
     9181 AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC
     9241 ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT
     9301 GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG
     9361 CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG
     9421 GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG
     9481 GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT  
//
