LOCUS       pHIT203        9188 bp ds-DNA     circular SYN 18-JUN-2023
DEFINITION  Bacterial expression of C04F12.1 with 6xHis and maltose binding 
            protein tags for in vitro assays
ACCESSION   .
VERSION     .
KEYWORDS    pHIT203
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1
  AUTHORS   Tabara H, Mitani S, Mochizuki M, Kohara Y, Nagata K
  TITLE     A small RNA system ensures accurate homologous pairing and unpaired
            silencing of meiotic chromosomes
  JOURNAL   EMBO J. 2023 Jun 1;42(11):e105002. doi: 10.15252/embj.2020105002.
   PUBMED   37078421
COMMENT     
ORIGIN
        1 taatacgact cactatagGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT
       61 TGTTTAACTT TAAGAAGGAG ATATACCatg GGCAGCAGCC ATCATCATCA TCATCACAGC
      121 AGCGGCCTGG TGCCGCGCGG CAGCCATAAT atgaaaactg aagaaggtaa actggtaatc
      181 tggattaacg gcgataaagg ctataacggt ctcgctgaag tcggtaagaa attcgagaaa
      241 gataccggaa ttaaagtcac cgttgagcat ccggataaac tggaagagaa attcccacag
      301 gttgcggcaa ctggcgatgg ccctgacatt atcttctggg cacacgaccg ctttggtggc
      361 tacgctcaat ctggcctgtt ggctgaaatc accccggaca aagcgttcca ggacaagctg
      421 tatccgttta cctgggatgc cgtacgttac aacggcaagc tgattgctta cccgatcgct
      481 gttgaagcgt tatcgctgat ttataacaaa gatctgctgc cgaacccgcc aaaaacctgg
      541 gaagagatcc cggcgctgga taaagaactg aaagcgaaag gtaagagcgc gctgatgttc
      601 aacctgcaag aaccgtactt cacctggccg ctgattgctg ctgacggggg ttatgcgttc
      661 aagtatgaaa acggcaagta cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa
      721 gcgggtctga ccttcctggt tgacctgatt aaaaacaaac acatgaatgc agacaccgat
      781 tactccatcg cagaagctgc ctttaataaa ggcgaaacag cgatgaccat caacggcccg
      841 tgggcatggt ccaacatcga caccagcaaa gtgaattatg gtgtaacggt actgccgacc
      901 ttcaagggtc aaccatccaa accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc
      961 agtccgaaca aagagctggc aaaagagttc ctcgaaaact atctgctgac tgatgaaggt
     1021 ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag cgctgaagtc ttacgaggaa
     1081 gagttggcga aagatccacg tattgccgcc actatggaaa acgcccagaa aggtgaaatc
     1141 atgccgaaca tcccgcagat gtccgctttc tggtatgccg tgcgtactgc ggtgatcaac
     1201 gccgccagcg gtcgtcagac tgtcgatgaa gccctgaaag acgcgcagac tCATATGGCG
     1261 GCCGCaaagg acgagcaatc agctccagta gttccaactc ctgaattgtc tcgtgtctct
     1321 gtttcctcca atcctccgac tccttctgca attgaaaaac ctgtccctct tcctgttcca
     1381 tcgaaaccat tgttcgagta cgaacctcca gcacaactgg aagctttggc taagaataag
     1441 cctgtgaaag tgacaaccaa ctcatacgaa cttgagttga agccaactcc tgtttttcgt
     1501 tacgacgtca gtgttctgaa gagctttgac gattcaacca gggatccaat tgagcttgct
     1561 ggctcgaaag gcggcgatcg tcagcggcaa gctgacttga cggaaatcgt gaaagttgct
     1621 ctcacattgg aaggccttgg aaatctgaac atcttctacg atggcgcagc gatgcttttc
     1681 acaactcaga aattcgaaac aaaaacggaa aagcagcaag caattttcat gccaattccg
     1741 tctgatcatc caaagctctc aacatctctc aaagaaagcc aattcggaag agcttccgga
     1801 aagttccacg tgtccatcga gttgaactcg aaacaatcgg aactaaatac aatcgatctt
     1861 ctcaatgaac gtctggattc agcagcttgt ccagtcactc agatggctca aatcgctctg
     1921 agtgcagagg ccaaacaaaa tggcttcatc attttggatg gaggacatga actcttcgat
     1981 cagaagaata caagaagaac aattggaaaa gatggagttg aggatatgga tggagtggct
     2041 gccagtataa aaatggctca aggaaataag aaaactggat ctgcacactt ggtcatggac
     2101 tacaaaaaga agcagttctt caagactggt ccattgaagg atttgataaa aggaatcaat
     2161 ccaaatcagc tccgttcata tctcaaaggt cttcgtgtcc acactacata ctctcatcaa
     2221 tcaatcttca ttgatggaat ttccaatgtc ccaatcggag aaattaaact tcccgatggt
     2281 actcctcttc ttgatgattg tgctcgagtt tccggaaagc ccaagagctt ttttgatgct
     2341 tccaagcctg cggttcaagt taaccagtac aacaaacggg aaggtagacg tatggtttat
     2401 agcttcccta tcgagaattt actggtaaaa ccaaaccaga aactgactca gaaccatgga
     2461 gaaccaccga gaagcatcaa gccggagctg agatttgatc tcataagaaa ggttggagag
     2521 tctgcaaagc ttctctctcc aaatcagaca ctgaaatcaa ttggtattgc gatcaatcca
     2581 gaaccgatcg ttgttgaagc tatgactgtg ccaaagccaa ccattctata caaagacgga
     2641 gcatttactt ctccagatct gttaaacaga acatcatggg acgttcaagc acgtaaccac
     2701 caatctctaa aaggcggatt tgtggaaccc atgaagatca acaagatctt gattctgtat
     2761 aatagtcaat cctacagtca agcaagtcca aaagaagcag ttgtcgaact gcaatcaatt
     2821 ctcagcaaaa aagccaaaga tgttggaatg acaattgggc aaattgatgt ggaagatctc
     2881 aatgatccgg atatcatcgg agcaattgaa aacaaaatga ccattctaaa aacgagccaa
     2941 gtcaagccaa ttgtgatcta cgccgatcat actagtgagg gtacccacag cactttgaag
     3001 cttcaggaac gattgtgtga ggtcataact caacaagtcg ctttagacaa atcattgaaa
     3061 agaacacctg gaaaagtgac agcaaacaac ttgttgatga agctcaatct gaagtatggt
     3121 ggcgtgaatc acaaagtgcg agtggacaat agcatttctc atctctgggg agacacttca
     3181 aacacactca tcatctcata tgatgtgtgc cattcttctg gaaaagtgta caagaaggat
     3241 gagatttgtg acgagccaag ctgcgttggt tttggattca atggaaccgc ttgccccgaa
     3301 gcattcattg gagatttcca ttaccagctc ccacgtcacg aacaagttga tgaacatctt
     3361 ctgaaattga gagccagatt catgctcaat cactatatta catctcgtaa gaagtatccc
     3421 caacaagtgg tcattttgcg cgacggagtc tctgaaggac aacacaaaat ggtcagaaac
     3481 gaagagttcc aggcgattaa acagagcatc atgaatgttt tcaatgaaaa gaaggcaaag
     3541 gctccgtctt ttgctcttct cgttgtcact aagcgtcatg ccaatcggct tttgattaaa
     3601 gatcagaatg gatatacgaa tgtcccaccg ttgacggcaa ttgaccaaac aattgtgaag
     3661 aagcaaggaa atgaagtcat ctttgtgtcc cattgtccac tcaatggtac cgcccaacca
     3721 attgtaatca atacattggt caacgatgga attttcaaga ccaatgatca attggtccag
     3781 ttcatcacag cactttgctg tgctcatcag aagtctacaa acatagtgtc tctcccagag
     3841 agtatctacg cagctgacga atacgccaaa agaggagccg atttgttcca gtcgtataaa
     3901 ctgaagcatc gagatcttcc aacgactacg atcgacgact cgacccagct cgactacgaa
     3961 aaaatcaccc taattctctg cttccaaacg agctctttca aaaataagag aattgcttaa
     4021 CCCGGGTCGA CTGCAGCGAT CGCTCGAGCA CCACCACCAC CACCACTGAG ATCCGGCTGC
     4081 TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AActagcata
     4141 accccttggg gcctctaaac gggtcttgag gggttttttg CTGAAAGGAG GAACTATATC
     4201 CGGATTGGCG AATGGGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT
     4261 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC
     4321 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT
     4381 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT
     4441 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC
     4501 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC
     4561 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG
     4621 ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGTTTACAAT TTCAGGTGGC
     4681 ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT
     4741 ATGTATCCGC TCATGAATTA ATTCTTAGAA AAACTCATCG AGCATCAAAT GAAACTGCAA
     4801 TTTATTCATA TCAGGATTAT CAATACCATA TTTTTGAAAA AGCCGTTTCT GTAATGAAGG
     4861 AGAAAACTCA CCGAGGCAGT TCCATAGGAT GGCAAGATCC TGGTATCGGT CTGCGATTCC
     4921 GACTCGTCCA ACATCAATAC AACCTATTAA TTTCCCCTCG TCAAAAATAA GGTTATCAAG
     4981 TGAGAAATCA CCATGAGTGA CGACTGAATC CGGTGAGAAT GGCAAAAGTT TATGCATTTC
     5041 TTTCCAGACT TGTTCAACAG GCCAGCCATT ACGCTCGTCA TCAAAATCAC TCGCATCAAC
     5101 CAAACCGTTA TTCATTCGTG ATTGCGCCTG AGCGAGACGA AATACGCGAT CGCTGTTAAA
     5161 AGGACAATTA CAAACAGGAA TCGAATGCAA CCGGCGCAGG AACACTGCCA GCGCATCAAC
     5221 AATATTTTCA CCTGAATCAG GATATTCTTC TAATACCTGG AATGCTGTTT TCCCGGGGAT
     5281 CGCAGTGGTG AGTAACCATG CATCATCAGG AGTACGGATA AAATGCTTGA TGGTCGGAAG
     5341 AGGCATAAAT TCCGTCAGCC AGTTTAGTCT GACCATCTCA TCTGTAACAT CATTGGCAAC
     5401 GCTACCTTTG CCATGTTTCA GAAACAACTC TGGCGCATCG GGCTTCCCAT ACAATCGATA
     5461 GATTGTCGCA CCTGATTGCC CGACATTATC GCGAGCCCAT TTATACCCAT ATAAATCAGC
     5521 ATCCATGTTG GAATTTAATC GCGGCCTAGA GCAAGACGTT TCCCGTTGAA TATGGCTCAT
     5581 AACACCCCTT GTATTACTGT TTATGTAAGC AGACAGTTTT ATTGTTCATG ACCAAAATCC
     5641 CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT
     5701 CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC
     5761 CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT
     5821 TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT
     5881 TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG
     5941 CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA
     6001 AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA
     6061 CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG
     6121 GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG
     6181 AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC
     6241 TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA
     6301 ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG
     6361 CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC
     6421 GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA
     6481 TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT
     6541 CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT
     6601 GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT
     6661 TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT
     6721 CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG
     6781 TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC
     6841 TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC
     6901 TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG
     6961 ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG
     7021 GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT
     7081 CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG
     7141 CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA
     7201 CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG
     7261 CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG
     7321 CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGG
     7381 GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG
     7441 ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC
     7501 GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT
     7561 CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC
     7621 GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG AGATCCCGGT
     7681 GCCTAATGAG TGAGCTAACT TACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG
     7741 GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG
     7801 CGTATTGGGC GCCAGGGTGG TTTTTCTTTT CACCAGTGAG ACGGGCAACA GCTGATTGCC
     7861 CTTCACCGCC TGGCCCTGAG AGAGTTGCAG CAAGCGGTCC ACGCTGGTTT GCCCCAGCAG
     7921 GCGAAAATCC TGTTTGATGG TGGTTAACGG CGGGATATAA CATGAGCTGT CTTCGGTATC
     7981 GTCGTATCCC ACTACCGAGA TATCCGCACC AACGCGCAGC CCGGACTCGG TAATGGCGCG
     8041 CATTGCGCCC AGCGCCATCT GATCGTTGGC AACCAGCATC GCAGTGGGAA CGATGCCCTC
     8101 ATTCAGCATT TGCATGGTTT GTTGAAAACC GGACATGGCA CTCCAGTCGC CTTCCCGTTC
     8161 CGCTATCGGC TGAATTTGAT TGCGAGTGAG ATATTTATGC CAGCCAGCCA GACGCAGACG
     8221 CGCCGAGACA GAACTTAATG GGCCCGCTAA CAGCGCGATT TGCTGGTGAC CCAATGCGAC
     8281 CAGATGCTCC ACGCCCAGTC GCGTACCGTC TTCATGGGAG AAAATAATAC TGTTGATGGG
     8341 TGTCTGGTCA GAGACATCAA GAAATAACGC CGGAACATTA GTGCAGGCAG CTTCCACAGC
     8401 AATGGCATCC TGGTCATCCA GCGGATAGTT AATGATCAGC CCACTGACGC GTTGCGCGAG
     8461 AAGATTGTGC ACCGCCGCTT TACAGGCTTC GACGCCGCTT CGTTCTACCA TCGACACCAC
     8521 CACGCTGGCA CCCAGTTGAT CGGCGCGAGA TTTAATCGCC GCGACAATTT GCGACGGCGC
     8581 GTGCAGGGCC AGACTGGAGG TGGCAACGCC AATCAGCAAC GACTGTTTGC CCGCCAGTTG
     8641 TTGTGCCACG CGGTTGGGAA TGTAATTCAG CTCCGCCATC GCCGCTTCCA CTTTTTCCCG
     8701 CGTTTTCGCA GAAACGTGGC TGGCCTGGTT CACCACGCGG GAAACGGTCT GATAAGAGAC
     8761 ACCGGCATAC TCTGCGACAT CGTATAACGT TACTGGTTTC ACATTCACCA CCCTGAATTG
     8821 ACTCTCTTCC GGGCGCTATC ATGCCATACC GCGAAAGGTT TTGCGCCATT CGATGGTGTC
     8881 CGGGATCTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT
     8941 TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA
     9001 GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA
     9061 AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC
     9121 CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC
     9181 CGCGAAAT     
//
