LOCUS       Exported                7652 bp ds-DNA     circular SYN 09-JUN-2023
DEFINITION  pGEX-6P-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..959
                     /organism="pGEX-6P-1"
                     /mol_type="other DNA"
                     /label=source:pGEX-6P-1
                     /note="/locus_tag=source:pGEX-6P-1"
     source          3642..7652
                     /organism="pGEX-6P-1"
                     /mol_type="other DNA"
                     /label=source:pGEX-6P-1
                     /label=source:pGEX-6P-1(1)
                     /note="/locus_tag=source:pGEX-6P-1(1)"
     CDS             82..959
                     /codon_start=1
                     /locus_tag="ORF frame 2"
                     /label=ORF frame 2
                     /translation="MTA*FVSLKAHSRSG*CFLRRHHNGSGKYSEMSC*QLIIGSYNVW
                     NCERITISHRKQYSCPLY*VIGKLRALCNPLDFFWNILKKNMKSICMSAMKVINGETKS
                     LNWVWSFPIFLIILMVMLN*HSLWPSYVI*LTSTTCWVVVQKSVQRFQCLKERFWILDT
                     VFRELHIVKTLKLSKLIFLASYLKC*KCSKIVYVIKHI*MVIM*PILTSCCMTLLMLFY
                     TWTQCAWMRSQN*FVLKNVLKLSHKLIST*NPASI*HGLCRAGKPRLVVATILQNRIWK
                     FCSRGPWDPRN"
     promoter        184..212
                     /locus_tag="tac_promoter"
                     /label=tac_promoter
     misc_feature    224..246
                     /locus_tag="M13_pUC_rev_primer"
                     /label=M13_pUC_rev_primer
     gene            258..959
                     /gene="GST"
                     /locus_tag="GST"
                     /label=GST
     CDS             258..959
                     /codon_start=1
                     /locus_tag="ORF frame 3"
                     /label=ORF frame 3
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLE
                     VLFQGPLGSPEF"
     misc_feature    918..938
                     /locus_tag="precision"
                     /label=precision
                     /note="/translation=MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGD
                     KWRNK 
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI 
                     RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD 
                     VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK 
                     SDLEVLFQGPLGSPEFPGRLERPHRD*"
     misc_feature    954..959
                     /locus_tag="EcoR I"
                     /label=EcoR I
     misc_feature    960..3641
                     /locus_tag="Hs.MVP"
                     /label=Hs.MVP
     CDS             3642..7205
                     /codon_start=1
                     /locus_tag="ORF frame 2(1)"
                     /label=ORF frame 2
                     /label=ORF frame 2(1)
                     /translation="MAAS*LTDDLPRAFR**R*KPLTHAAPGDGHSLSVSGCREQTSPS
                     GRVSGCWRVSGRSHDPVT*R*RSV*FLKTKGPRDTPIFIG*CHDNNGFLDVRWHFSGKC
                     ARNPYLFIFLNTFKYVSAHETITLINASIILKKEEYEYSTFPCRPYSLFCGILPSCFCS
                     PRNAGESKRC*RSVGCTSGLHRTGSQQR*DP*EFSPRRTFSNDEHF*SSAMWRGIIPC*
                     RRARATRSPHTLFSE*LG*VLTSHRKASYGWHDSKRIMQCCHNHE**HCGQLTSDNDRR
                     TEGANRFFAQHGGSCNSP*SLGTGAE*SHTKRRA*HHDACSNGNNVAQTINWRTTYSSF
                     PATINRLDGGG*SCRTTSALGPSGWLVYC**IWSR*AWVSRYHCSTGARW*ALPYRSYL
                     HDGESGNYG*TK*TDR*DRCLTD*ALVTVRPSLLIYTLD*FKTSFLI*KDLGEDPF**S
                     HDQNPLT*VFVPLSVRPRRKDQRIFLRSFFSARNLLLANKKTTATSGGLFAGSRATNSF
                     SEGNWLQQSADTKYCPSSVAVVRPPLQELCSTAYIPRSANPVTSGCCQWR*VVSYRVGL
                     KTIVTG*GAAVGLNGGFVHTAQLGANDLHRTEIPTA*AMRKRHASRREKGGQVSGKRQG
                     RNRRAHEGASRGKRLVSL*SCRVSPPLT*ASIFVMLVRGAEPMEKRQQRGLFTVPGLLL
                     AFCSHVLSCVIP*FCG*PYYRL*VS*YRSPQPNDRAQRVSERGSGRAPDAVFSPYASVR
                     YFTPHKFRHHRMVQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSDRFPRGEP
                     GQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAVANCRGD*I
                     SRRSTGCQRGGVDGRTKRRRSL*SGGAQSSRATRQWADH*LSAG*PGCHCCGSCLH*CS
                     GVIS*CL*PDTHQQYYFLP*RRYATGRGASGRIGSPANRAVSGPIKFCLGASASGWLA*
                     ISHSQSNSADSGTGRRLECHVRFSTNHANAE*GHRSHCDAGCQRSDGAGRNARHYRVRA
                     ARWCGYLGSGIRRYRRQLMLYPAVNHHQTGFSPAGANQRGPLAATLSGPGGEGQSAVAR
                     LTGEKKNHPGAQYANRLSPRVGRFINAAGTTGFPTGKRAVSATQLM*VSSLIRHPRLYT
                     LCFRLVCCVEL*ADNNFTQETA"
     gene            3642..3660
                     /gene="GST"
                     /locus_tag="GST(1)"
                     /label=GST
                     /label=GST(1)
     CDS             3642..3660
                     /codon_start=1
                     /locus_tag="ORF frame 3(3)"
                     /label=ORF frame 3
                     /label=ORF frame 3(3)
                     /translation="MAAS*L"
     misc_feature    3642..3649
                     /locus_tag="Not I"
                     /label=Not I
     misc_feature    complement(3702..3724)
                     /locus_tag="pGEX_3_primer"
                     /label=pGEX_3_primer
                     /note="/translation=MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGD
                     KWRNK 
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI 
                     RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD 
                     VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK 
                     SDLEVLFQGPLGSPEFPGRLERPHRD*"
     promoter        3990..4018
                     /locus_tag="AmpR_promoter"
                     /label=AmpR_promoter
                     /note="/translation=MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGD
                     KWRNK 
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI 
                     RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD 
                     VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK 
                     SDLEVLFQGPLGSPEFPGRLERPHRD*"
     gene            4060..4920
                     /gene="Ampicillin"
                     /locus_tag="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGD
                     KWRNK 
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI 
                     RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD 
                     VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK 
                     SDLEVLFQGPLGSPEFPGRLERPHRD*"
     CDS             4060..4920
                     /codon_start=1
                     /locus_tag="ORF frame 3(1)"
                     /label=ORF frame 3
                     /label=ORF frame 3(1)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5075..5694
                     /locus_tag="pBR322_origin"
                     /label=pBR322_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    5992..7083
                     /locus_tag="lacI"
                     /label=lacI
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     CDS             6124..7083
                     /codon_start=1
                     /locus_tag="ORF frame 3(2)"
                     /label=ORF frame 3
                     /label=ORF frame 3(2)
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSR
                     ADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPA
                     LFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKY
                     LTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLR
                     VGADISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVS
                     LVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ"
     promoter        7132..7161
                     /locus_tag="lac_promoter"
                     /label=lac_promoter
                     /note="/translation=MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIV
                     AAIKS 
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV 
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW 
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE 
                     SGLRVGADISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ 
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     misc_feature    7175..7197
                     /locus_tag="M13_pUC_rev_primer(1)"
                     /label=M13_pUC_rev_primer
                     /label=M13_pUC_rev_primer(1)
                     /note="/translation=MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIV
                     AAIKS 
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV 
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW 
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE 
                     SGLRVGADISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ 
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     promoter        7196..7214
                     /locus_tag="M13_reverse_primer"
                     /label=M13_reverse_primer
                     /note="/translation=MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIV
                     AAIKS 
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV 
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW 
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE 
                     SGLRVGADISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ 
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     misc_feature    7223..7378
                     /locus_tag="lacZ_a"
                     /label=lacZ_a
                     /note="/translation=MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRN
                     SEEAR 
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT 
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGITLSTARCTNAS 
                     GVRQPSEAVVWLCRS*"
     promoter        complement(7226..7242)
                     /locus_tag="M13_forward20_primer"
                     /label=M13_forward20_primer
                     /note="/translation=MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRN
                     SEEAR 
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT 
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGITLSTARCTNAS 
                     GVRQPSEAVVWLCRS*"
     misc_feature    complement(7235..7257)
                     /locus_tag="M13_pUC_fwd_primer"
                     /label=M13_pUC_fwd_primer
                     /note="/translation=MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRN
                     SEEAR 
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT 
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGITLSTARCTNAS 
                     GVRQPSEAVVWLCRS*"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
      901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc ccggaattca
      961 tggcaactga agagttcatc atccgcatcc ccccatacca ctatatccat gtgctggacc
     1021 agaacagcaa cgtgtcccgt gtggaggtcg ggccaaagac ctacatccgg caggacaatg
     1081 agagggtact gtttgccccc atgcgcatgg tgaccgtccc cccacgtcac tactgcacag
     1141 tggccaaccc tgtgtctcgg gatgcccagg gcttggtgct gtttgatgtc acagggcaag
     1201 ttcggcttcg ccacgctgac ctcgagatcc ggctggccca ggaccccttc cccctgtacc
     1261 caggggaggt gctggaaaag gacatcacac ccctgcaggt ggttctgccc aacactgccc
     1321 tccatctaaa ggcgctgctt gattttgagg ataaagatgg agacaaggtg gtggcaggag
     1381 atgagtggct tttcgaggga cctggcacgt acatcccccg gaaggaagtg gaggtcgtgg
     1441 agatcattca ggccaccatc atcaggcaga accaggctct gcggctcagg gcccgcaagg
     1501 agtgctggga ccgggacggc aaggagaggg tgacagggga agaatggctg gtcaccacag
     1561 taggggcgta cctcccagcg gtgtttgagg aggttctgga tttggtggac gccgtcatcc
     1621 ttacggaaaa gacagccctg cacctccggg ctcggcggaa cttccgggac ttcaggggag
     1681 tgtcccgccg cactggggag gagtggctgg taacagtgca ggacacagag gcccacgtgc
     1741 cagatgtcca cgaggaggtg ctgggggttg tgcccatcac caccctgggc ccccacaact
     1801 actgcgtgat tctcgaccct gtcggaccgg atggcaagaa tcagctgggg cagaagcgcg
     1861 tggtcaaggg agagaagtct tttttcctcc agccaggaga gcagctggaa caaggcatcc
     1921 aggatgtgta tgtgctgtcg gagcagcagg ggctgctgct gagggccctg cagcccctgg
     1981 aggaggggga ggatgaggag aaggtctcac accaggctgg ggaccactgg ctcatccgcg
     2041 gacccctgga gtatgtgcca tctgccaaag tggaggtggt ggaggagcgc caggccatcc
     2101 ctctagacga gaacgagggc atctatgtgc aggatgtcaa gaccggaaag gtgcgcgctg
     2161 tgattggaag cacctacatg ctgacccagg acgaagtcct gtgggagaaa gagctgcctc
     2221 ccggggtgga ggagctgctg aacaaggggc aggaccctct ggcagacagg ggtgagaagg
     2281 acacagctaa gagcctccag cccttggcgc cccggaacaa gacccgtgtg gtcagctacc
     2341 gcgtgcccca caacgctgcg gtgcaggtgt acgactaccg agagaagcga gcccgcgtgg
     2401 tcttcgggcc tgagctggtg tcgctgggtc ctgaggagca gttcacagtg ttgtccctct
     2461 cagctgggcg gcccaagcgt ccccatgccc gccgtgcgct ctgcctgctg ctggggcctg
     2521 acttcttcac agacgtcatc accatcgaaa cggcggatca tgccaggctg caactgcagc
     2581 tggcctacaa ctggcacttt gaggtgaatg accggaagga cccccaagag acggccaagc
     2641 tcttttcagt gccagacttt gtaggtgatg cctgcaaagc catcgcatcc cgggtgcggg
     2701 gggccgtggc ctctgtcact ttcgatgact tccataagaa ctcagcccgc atcattcgca
     2761 ctgctgtctt tggctttgag acctcggaag cgaagggccc cgatggcatg gccctgccca
     2821 ggccccggga ccaggctgtc ttcccccaaa acgggctggt ggtcagcagt gtggacgtgc
     2881 agtcagtgga gcctgtggat cagaggaccc gggacgccct gcaacgcagc gtccagctgg
     2941 ccatcgagat caccaccaac tcccaggaag cggcggccaa gcatgaggct cagagactgg
     3001 agcaggaagc ccgcggccgg cttgagcggc agaagatcct ggaccagtca gaagccgaga
     3061 aagctcgcaa ggaacttttg gagctggagg ctctgagcat ggccgtggag agcaccggga
     3121 ctgccaaggc ggaggccgag tcccgtgcgg aggcagcccg gattgaggga gaagggtccg
     3181 tgctgcaggc caagctaaaa gcacaggcct tggccattga aacggaggct gagctccaga
     3241 gggtccagaa ggtccgagag ctggaactgg tctatgcccg ggcccagctg gagctggagg
     3301 tgagcaaggc tcagcagctg gctgaggtgg aggtgaagaa gttcaagcag atgacagagg
     3361 ccataggccc cagcaccatc agggaccttg ctgtggctgg gcctgagatg caggtaaaac
     3421 tgctccagtc cctgggcctg aaatcaaccc tcatcaccga tggctccact cccatcaacc
     3481 tcttcaacac agcctttggg ctgctgggga tggggcccga gggtcagccc ctgggcagaa
     3541 gggtggccag tgggcccagc cctggggagg ggatatcccc ccagtctgct caggcccctc
     3601 aagctcctgg agacaaccac gtggtgcctg tactgcgcta agcggccgca tcgtgactga
     3661 ctgacgatct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     3721 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     3781 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     3841 ggagtgtata attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     3901 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3961 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4021 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4081 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4141 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4201 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4261 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     4321 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4381 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4441 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4501 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4561 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     4621 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4681 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4741 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4801 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4861 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4921 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4981 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5041 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5101 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5161 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5221 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     5281 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5341 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5401 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5461 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5521 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5581 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5641 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5701 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5761 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     5821 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     5881 ctccttacgc atctgtgcgg tatttcacac cgcataaatt ccgacaccat cgaatggtgc
     5941 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat
     6001 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
     6061 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
     6121 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
     6181 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
     6241 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
     6301 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
     6361 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
     6421 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
     6481 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
     6541 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
     6601 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
     6661 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
     6721 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
     6781 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
     6841 tgttatatcc cgccgtcaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
     6901 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
     6961 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
     7021 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     7081 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     7141 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     7201 cagctatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     7261 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     7321 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     7381 ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc
     7441 ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc
     7501 ccatctacac caacgtaacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga
     7561 atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc
     7621 agacgcgaat tatttttgat ggcgttggaa tt
//
