LOCUS       Exported                7601 bp ds-DNA     circular SYN 09-JUN-2023
DEFINITION  pGEX-6P-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..959
                     /organism="pGEX-6P-1"
                     /mol_type="other DNA"
                     /label=source:pGEX-6P-1
                     /note="/locus_tag=source:pGEX-6P-1"
     source          3591..7601
                     /organism="pGEX-6P-1"
                     /mol_type="other DNA"
                     /label=source:pGEX-6P-1
                     /label=source:pGEX-6P-1(1)
                     /note="/locus_tag=source:pGEX-6P-1(1)"
     CDS             82..959
                     /codon_start=1
                     /locus_tag="ORF frame 2"
                     /label=ORF frame 2
                     /translation="MTA*FVSLKAHSRSG*CFLRRHHNGSGKYSEMSC*QLIIGSYNVW
                     NCERITISHRKQYSCPLY*VIGKLRALCNPLDFFWNILKKNMKSICMSAMKVINGETKS
                     LNWVWSFPIFLIILMVMLN*HSLWPSYVI*LTSTTCWVVVQKSVQRFQCLKERFWILDT
                     VFRELHIVKTLKLSKLIFLASYLKC*KCSKIVYVIKHI*MVIM*PILTSCCMTLLMLFY
                     TWTQCAWMRSQN*FVLKNVLKLSHKLIST*NPASI*HGLCRAGKPRLVVATILQNRIWK
                     FCSRGPWDPRN"
     promoter        184..212
                     /locus_tag="tac_promoter"
                     /label=tac_promoter
     misc_feature    224..246
                     /locus_tag="M13_pUC_rev_primer"
                     /label=M13_pUC_rev_primer
     gene            258..959
                     /gene="GST"
                     /locus_tag="GST"
                     /label=GST
     CDS             258..959
                     /codon_start=1
                     /locus_tag="ORF frame 3"
                     /label=ORF frame 3
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLE
                     VLFQGPLGSPEF"
     misc_feature    918..938
                     /locus_tag="precision"
                     /label=precision
                     /note="/translation=MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGD
                     KWRNK 
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI 
                     RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD 
                     VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK 
                     SDLEVLFQGPLGSPEFPGRLERPHRD*"
     misc_feature    954..959
                     /locus_tag="EcoR I"
                     /label=EcoR I
     misc_feature    960..3590
                     /locus_tag="Hs.MVP"
                     /label=Hs.MVP
     CDS             3591..7154
                     /codon_start=1
                     /locus_tag="ORF frame 2(1)"
                     /label=ORF frame 2
                     /label=ORF frame 2(1)
                     /translation="MAAS*LTDDLPRAFR**R*KPLTHAAPGDGHSLSVSGCREQTSPS
                     GRVSGCWRVSGRSHDPVT*R*RSV*FLKTKGPRDTPIFIG*CHDNNGFLDVRWHFSGKC
                     ARNPYLFIFLNTFKYVSAHETITLINASIILKKEEYEYSTFPCRPYSLFCGILPSCFCS
                     PRNAGESKRC*RSVGCTSGLHRTGSQQR*DP*EFSPRRTFSNDEHF*SSAMWRGIIPC*
                     RRARATRSPHTLFSE*LG*VLTSHRKASYGWHDSKRIMQCCHNHE**HCGQLTSDNDRR
                     TEGANRFFAQHGGSCNSP*SLGTGAE*SHTKRRA*HHDACSNGNNVAQTINWRTTYSSF
                     PATINRLDGGG*SCRTTSALGPSGWLVYC**IWSR*AWVSRYHCSTGARW*ALPYRSYL
                     HDGESGNYG*TK*TDR*DRCLTD*ALVTVRPSLLIYTLD*FKTSFLI*KDLGEDPF**S
                     HDQNPLT*VFVPLSVRPRRKDQRIFLRSFFSARNLLLANKKTTATSGGLFAGSRATNSF
                     SEGNWLQQSADTKYCPSSVAVVRPPLQELCSTAYIPRSANPVTSGCCQWR*VVSYRVGL
                     KTIVTG*GAAVGLNGGFVHTAQLGANDLHRTEIPTA*AMRKRHASRREKGGQVSGKRQG
                     RNRRAHEGASRGKRLVSL*SCRVSPPLT*ASIFVMLVRGAEPMEKRQQRGLFTVPGLLL
                     AFCSHVLSCVIP*FCG*PYYRL*VS*YRSPQPNDRAQRVSERGSGRAPDAVFSPYASVR
                     YFTPHKFRHHRMVQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSDRFPRGEP
                     GQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAVANCRGD*I
                     SRRSTGCQRGGVDGRTKRRRSL*SGGAQSSRATRQWADH*LSAG*PGCHCCGSCLH*CS
                     GVIS*CL*PDTHQQYYFLP*RRYATGRGASGRIGSPANRAVSGPIKFCLGASASGWLA*
                     ISHSQSNSADSGTGRRLECHVRFSTNHANAE*GHRSHCDAGCQRSDGAGRNARHYRVRA
                     ARWCGYLGSGIRRYRRQLMLYPAVNHHQTGFSPAGANQRGPLAATLSGPGGEGQSAVAR
                     LTGEKKNHPGAQYANRLSPRVGRFINAAGTTGFPTGKRAVSATQLM*VSSLIRHPRLYT
                     LCFRLVCCVEL*ADNNFTQETA"
     gene            3591..3609
                     /gene="GST"
                     /locus_tag="GST(1)"
                     /label=GST
                     /label=GST(1)
     CDS             3591..3609
                     /codon_start=1
                     /locus_tag="ORF frame 3(3)"
                     /label=ORF frame 3
                     /label=ORF frame 3(3)
                     /translation="MAAS*L"
     misc_feature    3591..3598
                     /locus_tag="Not I"
                     /label=Not I
     misc_feature    complement(3651..3673)
                     /locus_tag="pGEX_3_primer"
                     /label=pGEX_3_primer
                     /note="/translation=MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGD
                     KWRNK 
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI 
                     RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD 
                     VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK 
                     SDLEVLFQGPLGSPEFPGRLERPHRD*"
     promoter        3939..3967
                     /locus_tag="AmpR_promoter"
                     /label=AmpR_promoter
                     /note="/translation=MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGD
                     KWRNK 
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI 
                     RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD 
                     VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK 
                     SDLEVLFQGPLGSPEFPGRLERPHRD*"
     gene            4009..4869
                     /gene="Ampicillin"
                     /locus_tag="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGD
                     KWRNK 
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI 
                     RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD 
                     VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK 
                     SDLEVLFQGPLGSPEFPGRLERPHRD*"
     CDS             4009..4869
                     /codon_start=1
                     /locus_tag="ORF frame 3(1)"
                     /label=ORF frame 3
                     /label=ORF frame 3(1)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5024..5643
                     /locus_tag="pBR322_origin"
                     /label=pBR322_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    5941..7032
                     /locus_tag="lacI"
                     /label=lacI
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     CDS             6073..7032
                     /codon_start=1
                     /locus_tag="ORF frame 3(2)"
                     /label=ORF frame 3
                     /label=ORF frame 3(2)
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSR
                     ADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPA
                     LFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKY
                     LTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLR
                     VGADISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVS
                     LVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ"
     promoter        7081..7110
                     /locus_tag="lac_promoter"
                     /label=lac_promoter
                     /note="/translation=MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIV
                     AAIKS 
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV 
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW 
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE 
                     SGLRVGADISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ 
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     misc_feature    7124..7146
                     /locus_tag="M13_pUC_rev_primer(1)"
                     /label=M13_pUC_rev_primer
                     /label=M13_pUC_rev_primer(1)
                     /note="/translation=MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIV
                     AAIKS 
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV 
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW 
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE 
                     SGLRVGADISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ 
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     promoter        7145..7163
                     /locus_tag="M13_reverse_primer"
                     /label=M13_reverse_primer
                     /note="/translation=MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIV
                     AAIKS 
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV 
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW 
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE 
                     SGLRVGADISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ 
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     misc_feature    7172..7327
                     /locus_tag="lacZ_a"
                     /label=lacZ_a
                     /note="/translation=MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRN
                     SEEAR 
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT 
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGITLSTARCTNAS 
                     GVRQPSEAVVWLCRS*"
     promoter        complement(7175..7191)
                     /locus_tag="M13_forward20_primer"
                     /label=M13_forward20_primer
                     /note="/translation=MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRN
                     SEEAR 
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT 
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGITLSTARCTNAS 
                     GVRQPSEAVVWLCRS*"
     misc_feature    complement(7184..7206)
                     /locus_tag="M13_pUC_fwd_primer"
                     /label=M13_pUC_fwd_primer
                     /note="/translation=MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRN
                     SEEAR 
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT 
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGITLSTARCTNAS 
                     GVRQPSEAVVWLCRS*"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
      901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc ccggaattca
      961 tggcaactga agagttcatc atccgcatcc ccccatacca ctatatccat gtgctggacc
     1021 agaacagcaa cgtgtcccgt gtggaggtcg ggccaaagac ctacatccgg caggacaatg
     1081 agagggtact gtttgccccc atgcgcatgg tgaccgtccc cccacgtcac tactgcacag
     1141 tggccaaccc tgtgtctcgg gatgcccagg gcttggtgct gtttgatgtc acagggcaag
     1201 ttcggcttcg ccacgctgac ctcgagatcc ggctggccca ggaccccttc cccctgtacc
     1261 caggggaggt gctggaaaag gacatcacac ccctgcaggt ggttctgccc aacactgccc
     1321 tccatctaaa ggcgctgctt gattttgagg ataaagatgg agacaaggtg gtggcaggag
     1381 atgagtggct tttcgaggga cctggcacgt acatcccccg gaaggaagtg gaggtcgtgg
     1441 agatcattca ggccaccatc atcaggcaga accaggctct gcggctcagg gcccgcaagg
     1501 agtgctggga ccgggacggc aaggagaggg tgacagggga agaatggctg gtcaccacag
     1561 taggggcgta cctcccagcg gtgtttgagg aggttctgga tttggtggac gccgtcatcc
     1621 ttacggaaaa gacagccctg cacctccggg ctcggcggaa cttccgggac ttcaggggag
     1681 tgtcccgccg cactggggag gagtggctgg taacagtgca ggacacagag gcccacgtgc
     1741 cagatgtcca cgaggaggtg ctgggggttg tgcccatcac caccctgggc ccccacaact
     1801 actgcgtgat tctcgaccct gtcggaccgg atggcaagaa tcagctgggg cagaagcgcg
     1861 tggtcaaggg agagaagtct tttttcctcc agccaggaga gcagctggaa caaggcatcc
     1921 aggatgtgta tgtgctgtcg gagcagcagg ggctgctgct gagggccctg cagcccctgg
     1981 aggaggggga ggatgaggag aaggtctcac accaggctgg ggaccactgg ctcatccgcg
     2041 gacccctgga gtatgtgcca tctgccaaag tggaggtggt ggaggagcgc caggccatcc
     2101 ctctagacga gaacgagggc atctatgtgc aggatgtcaa gaccggaaag gtgcgcgctg
     2161 tgattggaag cacctacatg ctgacccagg acgaagtcct gtgggagaaa gagctgcctc
     2221 ccggggtgga ggagctgctg aacaaggggc aggaccctct ggcagacagg ggtgagaagg
     2281 acacagctaa gagcctccag cccttggcgc cccggaacaa gacccgtgtg gtcagctacc
     2341 gcgtgcccca caacgctgcg gtgcaggtgt acgactaccg agagaagcga gcccgcgtgg
     2401 tcttcgggcc tgagctggtg tcgctgggtc ctgaggagca gttcacagtg ttgtccctct
     2461 cagctgggcg gcccaagcgt ccccatgccc gccgtgcgct ctgcctgctg ctggggcctg
     2521 acttcttcac agacgtcatc accatcgaaa cggcggatca tgccaggctg caactgcagc
     2581 tggcctacaa ctggcacttt gaggtgaatg accggaagga cccccaagag acggccaagc
     2641 tcttttcagt gccagacttt gtaggtgatg cctgcaaagc catcgcatcc cgggtgcggg
     2701 gggccgtggc ctctgtcact ttcgatgact tccataagaa ctcagcccgc atcattcgca
     2761 ctgctgtctt tggctttgac caggctgtct tcccccaaaa cgggctggtg gtcagcagtg
     2821 tggacgtgca gtcagtggag cctgtggatc agaggacccg ggacgccctg caacgcagcg
     2881 tccagctggc catcgagatc accaccaact cccaggaagc ggcggccaag catgaggctc
     2941 agagactgga gcaggaagcc cgcggccggc ttgagcggca gaagatcctg gaccagtcag
     3001 aagccgagaa agctcgcaag gaacttttgg agctggaggc tctgagcatg gccgtggaga
     3061 gcaccgggac tgccaaggcg gaggccgagt cccgtgcgga ggcagcccgg attgagggag
     3121 aagggtccgt gctgcaggcc aagctaaaag cacaggcctt ggccattgaa acggaggctg
     3181 agctccagag ggtccagaag gtccgagagc tggaactggt ctatgcccgg gcccagctgg
     3241 agctggaggt gagcaaggct cagcagctgg ctgaggtgga ggtgaagaag ttcaagcaga
     3301 tgacagaggc cataggcccc agcaccatca gggaccttgc tgtggctggg cctgagatgc
     3361 aggtaaaact gctccagtcc ctgggcctga aatcaaccct catcaccgat ggctccactc
     3421 ccatcaacct cttcaacaca gcctttgggc tgctggggat ggggcccgag ggtcagcccc
     3481 tgggcagaag ggtggccagt gggcccagcc ctggggaggg gatatccccc cagtctgctc
     3541 aggcccctca agctcctgga gacaaccacg tggtgcctgt actgcgctaa gcggccgcat
     3601 cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     3661 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     3721 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     3781 agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     3841 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     3901 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3961 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4021 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4081 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4141 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4201 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     4261 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4321 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4381 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4441 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4501 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     4561 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4621 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4681 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4741 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4801 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4861 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4921 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4981 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5041 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5101 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5161 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     5221 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5281 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5341 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5401 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5461 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5521 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5581 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5641 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5701 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5761 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     5821 cggtattttc tccttacgca tctgtgcggt atttcacacc gcataaattc cgacaccatc
     5881 gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg
     5941 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
     6001 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
     6061 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
     6121 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
     6181 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
     6241 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
     6301 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
     6361 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
     6421 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
     6481 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
     6541 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
     6601 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
     6661 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
     6721 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
     6781 gacagctcat gttatatccc gccgtcaacc accatcaaac aggattttcg cctgctgggg
     6841 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
     6901 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     6961 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     7021 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     7081 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     7141 cacaggaaac agctatgacc atgattacgg attcactggc cgtcgtttta caacgtcgtg
     7201 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     7261 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     7321 atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg
     7381 agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt
     7441 acgatgcgcc catctacacc aacgtaacct atcccattac ggtcaatccg ccgtttgttc
     7501 ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac
     7561 aggaaggcca gacgcgaatt atttttgatg gcgttggaat t
//
